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Citations for LifeSensors :
1 - 30 of 30 citations for Ubiquitin Associated Protein 2 Like UBAP2L Antibody HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... The plasmid containing the gene for human γS WT was engineered into the pE-SUMO (Small Ubiquitin-like Modifier) vector containing a N-terminal 6XHis tagged fusion protein (LifeSensors Inc., Malvern, PA). The γS N14D and γS N76D were generated via site-directed mutagenesis using QuikChangeXL Kit (Agilent Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... 2-10 µM of ubiquitin (LifeSensors si201), 10 µg of isolated ribosomes ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 papain-like protease (DB604) was purchased from Lifesensors (Malvern, PA). HCoV-HKU1 coronavirus nucleocapsid protein ...
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bioRxiv - Biochemistry 2021Quote: ... WT ubiquitin was produced in-house while single lysine ubiquitin and K0 ubiquitin were obtained from LifeSensors (Cat. # SI210).
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bioRxiv - Cell Biology 2021Quote: ... VU101: Anti-ubiquitin Antibody (VU-0101, 1:1000) was purchased from LifeSensors (PA, USA). Anti-mouse HRP conjugate (W4021 ...
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bioRxiv - Cell Biology 2024Quote: ... Ubiquitinated proteins were pulled down from yeast lysates using Tandem Ubiquitin Binding Entities (TUBEs) (LifeSensors, UM402) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... anti-Ubiquitin (1:200; LifeSensors, AB120), and anti-Vimentin (1:400 ...
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bioRxiv - Cell Biology 2022Quote: Total Poly-ubiquitinated proteins were isolated using a LifeSensors Tandem Ubiquitin Binding Entities (TUBEs) Kit (LifeSensors, UM411M) and a modified protocol ...
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bioRxiv - Cell Biology 2023Quote: ... and ubiquitin (Vu1, LifeSensors, catalog no. VU101). Images were acquired using a Gbox imaging system (Syngene ...
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bioRxiv - Molecular Biology 2022Quote: ... Immunoblotting was performed using the following primary antibodies: anti-ubiquitin (1:10,000; LifeSensors; MAb; clone VU-1), anti-K48 (1:10,000 ...
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bioRxiv - Biochemistry 2022Quote: ... Ubiquitin transfer from HOIP RBR to the substrate (TAMRA-ubiquitin) was performed on ice and induced by addition of 4 µM fluorescent TAMRA-ubiquitin (LifeSensors SI270T ...
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bioRxiv - Cancer Biology 2021Quote: ... Ubiquitin reagent TUBE1 (UM101) was obtained from LifeSensors, Inc ...
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bioRxiv - Cell Biology 2020Quote: ... and immunoblotting was performed using the following primary antibodies: anti-ubiquitin (1:10,000; LifeSensors; MAb; clone VU-1), anti-K48 (1:10,000 ...
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bioRxiv - Cell Biology 2022Quote: ... anti-ubiquitin (1:10,000; LifeSensors; Mab; clone VU-1), anti-K63 (1:1,000 ...
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bioRxiv - Immunology 2020Quote: ... K63-linked and linear tetra-ubiquitin were obtained from LifeSensors. Caspase 8 siRNA was from Dharmacon ...
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bioRxiv - Genetics 2020Quote: Tandem Ubiquitin Binding entities (TUBE)-based mass spectrometry was performed by LifeSensors. The company provided the number of peptides detected ...
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bioRxiv - Cell Biology 2020Quote: ... The pull-down of ubiquitinated proteins from protein extracts was performed with TUBE 2 agarose beads (LifeSensors), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and then incubated with 30 μl of K63-Tandem Ubiquitin Binding Entity (TUBE)-Agarose (LifeSensors, UM401), or 2.4 μg of K63-TUBE-Biotin (LifeSensors ...
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bioRxiv - Molecular Biology 2023Quote: ... 600 µg of WCE was mixed with 2 µg of α-FLAG antibody in a total volume of 400 µL WCE buffer that had been mock treated or treated with 3 µg of K48ub or K63ub linkage specific Tandem Ubiquitin Binding Entities (TUBEs) purchased from LifeSensors. Samples were rotated at 4°C for a minimum of two hours before adding Protein A agarose beads and rotating at 4°C for an additional one hour to capture the immune complexes ...
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bioRxiv - Neuroscience 2022Quote: ... 2 mg of total protein extract was incubated with 100 μL of pre-equilibrated Agarose-TUBEs (LifeSensors), overnight at 4°C on a rocking platform ...
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bioRxiv - Biochemistry 2022Quote: ... one with a 50:50 mix of the tanespimycin and mock-treated HT29 lysates (1 mg total protein) in the presence of control magnetic beads without any conjugated antibody (LifeSensors, catalog no. UM400M) (‘–IgG’) ...
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bioRxiv - Microbiology 2020Quote: ... was added to sample 2 (DUBPAN) and 2 μl of OTUB1 (LifeSensors, Cat#: DB201) was added to sample 3 (DUBK48) ...
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bioRxiv - Plant Biology 2023Quote: ... Arabidopsis KAI2 protein was expressed as a 6× His-SUMO fusion protein using the expression vector pSUMO (LifeSensors). His-SUMO-KAI2 was isolated from by Ni-NTA resin and the eluted His-SUMO KAI2 was further separated by anion-exchange ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... 40 μl of Agarose-TUBEs 2 (UM402, LifeSensors) previously equilibrated in JS buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... 1mg protein was added to 20μl of TUBE1 agarose (LifeSensors) and incubated for 2 hours at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Cold TBST-washed pan-selective TUBE 2 beads (LifeSensors #UM-0402M) were incubated with neuron lysates overnight at 4°C with rotation ...
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bioRxiv - Cell Biology 2020Quote: Ubiquitinated proteins were analysed using TUBE2 (Agarose, 30 µl per sample, UM402, LifeSensors), TUBE1 (magnetic beads ...
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bioRxiv - Neuroscience 2022Quote: ... 850 µg protein was incubated with 80 µl equilibrated TUBE1 magnetic beads (LifeSensors) at 4 degrees Celsius for 2 hours ...
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bioRxiv - Immunology 2021Quote: ... mice were injected intraperitoneally with 25 µg recombinant SARS-CoV-2 S2 (CV2006; LifeSensors) in monophosphoryl lipid A adjuvant (Sigma Adjuvant System ...
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bioRxiv - Biophysics 2020Quote: ... SARS CoV-2 Mpro gene was subcloned from pET29a(+) to pE-SUMO vector according to manufacturer’s protocol (LifeSensors Inc, Malvern PA). pE-SUMO plasmid with SARS CoV-2 Main protease gene (Mpro ...