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1 - 12 of 12 citations for 8 CHLORO 2 THIEN 2 YLQUINOLINE 4 CARBOXYLIC ACID since 2019

• Viral-mediated ubiquitination impacts interactions of host proteins with viral RNA and promotes viral RNA processing

  Christin Herrmann, et al., bioRxiv - Microbiology 2020

  Quote: ... was added to sample 2 (DUBPAN) and 2 μl of OTUB1 (LifeSensors, Cat#: DB201) was added to sample 3 (DUBK48) ...
• Redox sensitive E2 Rad6 controls cellular response to oxidative stress via K63 ubiquitination of ribosomes

  Vanessa Simões, et al., bioRxiv - Cell Biology 2021

  Quote: ... 2-10 µM of ubiquitin (LifeSensors si201), 10 µg of isolated ribosomes ...
• The ubiquitin ligase Hecw controls oogenesis and neuronal homeostasis by promoting the liquid state of ribonucleoprotein particles

  Valentina Fajner, et al., bioRxiv - Animal Behavior and Cognition 2020

  Quote: ... 40 μl of Agarose-TUBEs 2 (UM402, LifeSensors) previously equilibrated in JS buffer ...
• The Hao-Fountain syndrome protein USP7 regulates neuronal connectivity in the brain via a novel p53-independent ubiquitin signaling pathway

  Hao Chen, et al., bioRxiv - Neuroscience 2023

  Quote: ... Cold TBST-washed pan-selective TUBE 2 beads (LifeSensors #UM-0402M) were incubated with neuron lysates overnight at 4°C with rotation ...
• Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin

  Yisong Qian, et al., bioRxiv - Immunology 2021

  Quote: ... SARS-CoV-2 papain-like protease (DB604) was purchased from Lifesensors (Malvern, PA). HCoV-HKU1 coronavirus nucleocapsid protein ...
• Broad human and animal coronavirus neutralisation by SARS-CoV-2 S2-targeted vaccination

  Kevin W. Ng, et al., bioRxiv - Immunology 2021

  Quote: ... mice were injected intraperitoneally with 25 µg recombinant SARS-CoV-2 S2 (CV2006; LifeSensors) in monophosphoryl lipid A adjuvant (Sigma Adjuvant System ...
• An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis

  Molly M. Wilson, et al., bioRxiv - Cell Biology 2020

  Quote: ... The pull-down of ubiquitinated proteins from protein extracts was performed with TUBE 2 agarose beads (LifeSensors), following the manufacturer’s instructions ...
• Regulation of neuronal mRNA splicing and Tau isoform ratio by ATXN3 through deubiquitylation of splicing factors

  Andreia Neves-Carvalho, et al., bioRxiv - Neuroscience 2022

  Quote: ... 2 mg of total protein extract was incubated with 100 μL of pre-equilibrated Agarose-TUBEs (LifeSensors), overnight at 4°C on a rocking platform ...
• Structure and inhibition of the SARS-CoV-2 main protease reveals strategy for developing dual inhibitors against M^pro and cathepsin L

  Michael Dominic Sacco, et al., bioRxiv - Biophysics 2020

  Quote: ... SARS CoV-2 Mpro gene was subcloned from pET29a(+) to pE-SUMO vector according to manufacturer’s protocol (LifeSensors Inc, Malvern PA). pE-SUMO plasmid with SARS CoV-2 Main protease gene (Mpro ...
• Ca²⁺-dependent liquid-liquid phase separation underlies intracellular Ca²⁺ stores

  Joshua E. Mayfield, et al., bioRxiv - Biochemistry 2021

  Quote: ... FAM20C (amino acids 93-584) was subcloned into a modified pI-secSUMOstar vector (LifeSensors, Malvern, PA) in which the original SUMO tag was replaced by a MBP tag and tobacco etch virus (TEV ...
• Hyperconcentrated Mucus Unifies Submucosal Gland and Superficial Airway Dysfunction in Cystic Fibrosis

  Takafumi Kato, et al., bioRxiv - Pathology 2021

  Quote: ... PRR4 cDNA without a signal peptide sequence (corresponding to amino acids 17 to 134) was cloned into the pM-secSUMOstar Vector (7121, LifeSensors). SUMOstar-PRR4 vectors were transfected into Expi293 cells (1 mg of DNA per liter of transfection ...
• The unifying catalytic mechanism of the RING-between-RING E3 ligase family

  Xiangyi S. Wang, et al., bioRxiv - Biochemistry 2022

  Quote: ... Ubiquitin transfer from HOIP RBR to the substrate (TAMRA-ubiquitin) was performed on ice and induced by addition of 4 µM fluorescent TAMRA-ubiquitin (LifeSensors SI270T ...