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Citations for LifeSensors :
1 - 39 of 39 citations for 7 7R 7 Amino 5 azaspiro 2.4 hept 5 yl 8 chloro 6 fluoro 1 1S 2R 2 fluorocyclopropyl 1 4 dihydro 4 oxo 3 quinolinecarboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... 5 (LifeSensors) FRET-K48 diubiquitin ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM β-Mercaptoethanol and 5% Glycerol] containing SUMO protease (LifeSensors) in order to cleave the SUMO tag and generate Pol κ in the native form ...
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bioRxiv - Biochemistry 2021Quote: ... FAM20C (amino acids 93-584) was subcloned into a modified pI-secSUMOstar vector (LifeSensors, Malvern, PA) in which the original SUMO tag was replaced by a MBP tag and tobacco etch virus (TEV ...
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bioRxiv - Biochemistry 2022Quote: ... 5 mM β-Mercaptoethanol and 5% Glycerol) in the presence of SUMO protease (LifeSensors) to remove the SUMO tag to generate native PolH ...
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bioRxiv - Cell Biology 2021Quote: ... or 2.4 μg of K63-TUBE-Biotin (LifeSensors, UM304) or M1-TUBE-Biotin (LifeSensors ...
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bioRxiv - Cell Biology 2019Quote: ... and 5 μM 1,10-phenanthroline (LifeSensors) were added in the lysis buffer for ubiquitin detection by western blotting ...
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bioRxiv - Pathology 2021Quote: ... PRR4 cDNA without a signal peptide sequence (corresponding to amino acids 17 to 134) was cloned into the pM-secSUMOstar Vector (7121, LifeSensors). SUMOstar-PRR4 vectors were transfected into Expi293 cells (1 mg of DNA per liter of transfection ...
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bioRxiv - Microbiology 2020Quote: ... 5 mM 1,10-phenanthroline (LifeSensors, Cat#: SI9649), and 1 μl/ml Benzonase (Sigma-Alrich) ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µg/mL PR-619 (SI9619, LifeSensors), 20 µM MG132 (474790 ...
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bioRxiv - Neuroscience 2024Quote: ... and 5 mM 1,10-phenanthroline (o-PA) (100X, #SI9649, LifeSensors). The total protein content of the lysates was determined by BCA protein assay (#23227 ...
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bioRxiv - Cell Biology 2022Quote: ... anti-ubiquitin (1:10,000; LifeSensors; Mab; clone VU-1), anti-K63 (1:1,000 ...
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bioRxiv - Biochemistry 2022Quote: ... Ubiquitin transfer from HOIP RBR to the substrate (TAMRA-ubiquitin) was performed on ice and induced by addition of 4 µM fluorescent TAMRA-ubiquitin (LifeSensors SI270T ...
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bioRxiv - Cell Biology 2023Quote: ... anti-Ubiquitin (1:200; LifeSensors, AB120), and anti-Vimentin (1:400 ...
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bioRxiv - Molecular Biology 2022Quote: ... Immunoblotting was performed using the following primary antibodies: anti-ubiquitin (1:10,000; LifeSensors; MAb; clone VU-1), anti-K48 (1:10,000 ...
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bioRxiv - Cell Biology 2020Quote: ... and immunoblotting was performed using the following primary antibodies: anti-ubiquitin (1:10,000; LifeSensors; MAb; clone VU-1), anti-K48 (1:10,000 ...
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bioRxiv - Microbiology 2020Quote: ... 1 μl of USP2 (LifeSensors, Cat#: DB501) was added to sample 2 (DUBPAN ...
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bioRxiv - Microbiology 2020Quote: ... was added to sample 2 (DUBPAN) and 2 μl of OTUB1 (LifeSensors, Cat#: DB201) was added to sample 3 (DUBK48) ...
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bioRxiv - Microbiology 2021Quote: ... It was cloned into pE-SUMOpro-3 plasmid (LifeSensors Inc.) between Nco I and Xho I restriction sites ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubating with either 2.5 µl (∼25 units) of SUMOstar Protease 1 (LifeSensors) for constructs in SUMOstar vectors or with SUMO Protease (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: The PCR product was cloned into pE-SUMOpro-3 plasmid (LifeSensors Inc) between Bsa I and Xho I restriction sites ...
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bioRxiv - Cell Biology 2021Quote: ... 2-10 µM of ubiquitin (LifeSensors si201), 10 µg of isolated ribosomes ...
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bioRxiv - Cell Biology 2021Quote: ... VU101: Anti-ubiquitin Antibody (VU-0101, 1:1000) was purchased from LifeSensors (PA, USA). Anti-mouse HRP conjugate (W4021 ...
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bioRxiv - Microbiology 2021Quote: ... The synthetic DNA fragments were cloned into pE-SUMOpro-3 plasmid (LifeSensors Inc.) between Kif I and Xho I sites ...
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bioRxiv - Plant Biology 2023Quote: ... Arabidopsis KAI2 protein was expressed as a 6× His-SUMO fusion protein using the expression vector pSUMO (LifeSensors). His-SUMO-KAI2 was isolated from by Ni-NTA resin and the eluted His-SUMO KAI2 was further separated by anion-exchange ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... 40 μl of Agarose-TUBEs 2 (UM402, LifeSensors) previously equilibrated in JS buffer ...
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bioRxiv - Biochemistry 2022Quote: ... coli strain BL21 (DE3) with an N-terminal (His)6-SUMO tag from a pE-SUMO-pro expression vector (LifeSensors). Cells were grown in 2YT media at 24°C to an OD600 of 0.8 and then induced with 0.1 mM isopropyl β-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Neuroscience 2023Quote: ... Cold TBST-washed pan-selective TUBE 2 beads (LifeSensors #UM-0402M) were incubated with neuron lysates overnight at 4°C with rotation ...
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bioRxiv - Biochemistry 2020Quote: ... cells were harvested and resuspended in TUBE lysis buffer (50mM Tris-HCl, pH 7.5, 0.15M NaCl, 1mM EDTA, 1% NP-40, 10% glycerol, published by LifeSensors, Inc.) with 1X protease inhibitor cocktail (Bimake ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 papain-like protease (DB604) was purchased from Lifesensors (Malvern, PA). HCoV-HKU1 coronavirus nucleocapsid protein ...
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bioRxiv - Biochemistry 2021Quote: ... 125 nM USP51–835 was added and the plate was incubated at room temperature for 1 hour before the addition of 200 nM IQF Ub2K48 (LifeSensors, DU4802). Following a 1-minute centrifugation at 250 g ...
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bioRxiv - Molecular Biology 2023Quote: ... 600 µg of WCE was mixed with 2 µg of α-FLAG antibody in a total volume of 400 µL WCE buffer that had been mock treated or treated with 3 µg of K48ub or K63ub linkage specific Tandem Ubiquitin Binding Entities (TUBEs) purchased from LifeSensors. Samples were rotated at 4°C for a minimum of two hours before adding Protein A agarose beads and rotating at 4°C for an additional one hour to capture the immune complexes ...
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bioRxiv - Immunology 2021Quote: ... mice were injected intraperitoneally with 25 µg recombinant SARS-CoV-2 S2 (CV2006; LifeSensors) in monophosphoryl lipid A adjuvant (Sigma Adjuvant System ...
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bioRxiv - Molecular Biology 2021Quote: ... The synthetic gene blocks encoding mutant nsp1 were ordered from Integrated DNA Technologies and cloned into pE-SUMOpro-3 plasmid (LifeSensors Inc) between Eco RI and Xho I restriction sites ...
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bioRxiv - Microbiology 2020Quote: ... LIM4 aa 213-280) were obtained as synthetic DNA fragments from IDT and cloned into pE-SUMOpro-3 plasmid (LifeSensors Inc.) as fusions with SUMO.
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bioRxiv - Microbiology 2020Quote: ... of strain 181/25 was expressed as a fusion with Twin-Strep-tag (GSWSHPQFEKGGGSGGGSGGGSWSHPQFEK) from pE-SUMOpro-3 plasmid (LifeSensors Inc.) in E ...
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bioRxiv - Biochemistry 2022Quote: ... one with a 50:50 mix of the tanespimycin and mock-treated HT29 lysates (1 mg total protein) in the presence of control magnetic beads without any conjugated antibody (LifeSensors, catalog no. UM400M) (‘–IgG’) ...
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bioRxiv - Cell Biology 2020Quote: ... The pull-down of ubiquitinated proteins from protein extracts was performed with TUBE 2 agarose beads (LifeSensors), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... 2 mg of total protein extract was incubated with 100 μL of pre-equilibrated Agarose-TUBEs (LifeSensors), overnight at 4°C on a rocking platform ...
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bioRxiv - Biophysics 2020Quote: ... SARS CoV-2 Mpro gene was subcloned from pET29a(+) to pE-SUMO vector according to manufacturer’s protocol (LifeSensors Inc, Malvern PA). pE-SUMO plasmid with SARS CoV-2 Main protease gene (Mpro ...