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Citations for Thermo Fisher :
1 - 16 of 16 citations for pET28a DsRed2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: The vectors pET28a (Invitrogen), pHStag (GenBank accession number ...
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bioRxiv - Microbiology 2024Quote: Plasmids of pET28a-phop or pET28a-phop derivative were first transformed into BL21(DE3) (Invitrogen, Inc.). Then ...
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bioRxiv - Biochemistry 2021Quote: ... cloned into a pET28a expression vector (Invitrogen), using NdeI and XhoI restriction sites ...
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bioRxiv - Biochemistry 2020Quote: ... was initially cloned into the pET28a vector (Invitrogen) as described (15) ...
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bioRxiv - Biochemistry 2023Quote: Plasmids of pET28a-his TEV-glnR or pET28a-his TEV-glnR derivative was firstly transformed into expression strain BL21(DE3) (Invitrogen, Inc.). then single colonies of the positive transformants were inoculated and amplified with 5 L LB broth supplemented with 50 μg/ml kanamycin at 37 °C with shaking ...
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bioRxiv - Microbiology 2020Quote: ... while bslO gene was cloned in pET28a vector (Invitrogen). The resulting plasmids encodes His6 tagged fusion proteins ...
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bioRxiv - Cell Biology 2022Quote: ... 2007) and DsRed2-Mito (Tanaka Bio) plasmids using a ratio of 7:3 using Lipofectamine LTX Plus (ThermoFisher, 15338100) for 5 hours ...
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bioRxiv - Developmental Biology 2021Quote: The construct bactin2:loxP-DsRed2-loxP-eGFP-T2A-wt1a was generated by Gateway cloning (MultiSite Gateway Three-Fragment Vector Construction Kit; Invitrogen). As destination vector pDestTol2pA2 was used ...
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bioRxiv - Biochemistry 2020Quote: ... METTL5 ORF cDNA was cloned into pHAGE or pET28a expression vector (Invitrogen), respectively ...
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bioRxiv - Microbiology 2023Quote: IL-33 (residues 112–270) and its R169A mutant were cloned into vector pET28a (Invitrogen) and were expressed with a TEV protease cleavable N-terminal His6 tag ...
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bioRxiv - Molecular Biology 2024Quote: After the pET28a-roGFP2-GPX fusion expression plasmids were transformed into Escherichia coli BL21 (DE3) (Fisher Scientific, Loughborough, UK), a pre-culture of 5 mL volume was grown at 37 °C overnight ...
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bioRxiv - Neuroscience 2020Quote: The cDNA sequence corresponding to amino acids 1-192 of rab26 was amplified by PCR and cloned into the pET28a (Invitrogen) vector for protein expression ...
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bioRxiv - Microbiology 2024Quote: ... Transgenbiotech) and the pET28a expression vector (obtained with the EasyPure Plasmid MiniPrep Kit) were digested with the NcoI (Thermo Fisher) and XhoI (Thermo Fisher ...
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bioRxiv - Biochemistry 2020Quote: ... soluble form of HO2 spanning residues 1-248 (the core of the protein) was expressed from the pET28a vector in BL21(DE) cells (Life Technologies) and purified by Ni-NTA– agarose affinity chromatography (Qiagen ...
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bioRxiv - Plant Biology 2022Quote: ... and KTI3 genes in pDonr207 were subcloned into a Gateway compatible pET28a destination vector via a LR® Gateway cloning kit (Thermo Fisher Scientific) (Earley ...
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bioRxiv - Biophysics 2021Quote: ... The PCR product was inserted into the pET28a(+) vector using NdeI and SalI restriction enzymes and T4 DNA ligase (Thermo Fisher Scientific, Waltham, MA) and subsequently transformed into chemically competent DH5α E ...