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Citations for Thermo Fisher :
1 - 41 of 41 citations for Norgestimate d3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: Chemical competent BL21(D3) cells (Invitrogen) were transformed with selected pPE2-Fab plasmids and grown on LBagar/Kanamycin/1% glucose plates ...
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bioRxiv - Cell Biology 2024Quote: ... for HCMEC/D3 or TrypLETM (12563011, ThermoFisher) for HBMVECs and iCE-BECs ...
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bioRxiv - Cell Biology 2024Quote: cyclin D3 (1:1000, Thermo Fisher Scientific MA5-12717)
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bioRxiv - Cancer Biology 2021Quote: ... 1:2000 Cyclin D3 from Thermo Fisher (Waltham, MA, USA), 1:1000 Cyclin D2 from Proteintech (Rosemont ...
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bioRxiv - Neuroscience 2024Quote: ... Texas Red™-conjugated dextran (TxR-d3, 3 kDa; ThermoFisher) at 0.05% in filtered artificial CSF (Tocris) ...
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bioRxiv - Neuroscience 2024Quote: ... for hCMEC/D3 cells or Geltrex matrix (ThermoFisher Scientific #A1413201). Cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized with 0.25% Triton-X in PBS for 10 minutes prior to antibody incubation ...
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bioRxiv - Neuroscience 2021Quote: ... NSC media was switched to “D3” differentiation media (DMEM/F12 Gibco #11330032 ...
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bioRxiv - Cell Biology 2021Quote: ... HBVP and hCMEC/D3 were detached by 0.05% trypsin/EDTA (ThermoFisher Scientific) and resuspended in warm OM ...
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bioRxiv - Neuroscience 2023Quote: ... Texas Red™-conjugated dextran (TxR-d3, 3 kDa; ThermoFisher Scientific, UK) at 0.05% in filtered artificial CSF (Tocris ...
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bioRxiv - Neuroscience 2022Quote: ... Transfections of hCMEC/D3 cell monolayers were performed using Lipofectamine reagent (Invitrogen, CA) as per manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... The hCMEC/D3 cells were detached with Trypsin-EDTA (TrypLE Express 1x, Gibco, Denmark) and passaged ...
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bioRxiv - Cell Biology 2021Quote: Whole cell lysates from hCMEC/D3 were prepared in RIPA lysis buffer (ThermoFisher Scientific) supplemented with cOmplet Protease Inhibitor Cocktail (Roche) ...
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bioRxiv - Bioengineering 2022Quote: ... total proteins from hCMEC/D3 cells were extracted using RIPA buffer (89900, Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... 6xHis-D3 Arg996Gln data collections were performed in a Titan Krios microscope (Thermo Fisher) at 0.83 Å/pix and 300 kV acceleration voltage using a K2 Summit 4k x 4k detector (Gatan) ...
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bioRxiv - Cancer Biology 2024Quote: HCMEC/D3 and GBM cell lysates were collected in RIPA Buffer (Thermo Fisher Scientific) supplemented with 1x protease/phosphatase inhibitor cocktail (Cell Signaling) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Electrophoresis was performed in an Owl™ D3-14 electrophoresis box (Thermo Scientific, MA, USA) containing 1X TBE buffer for 45 min at 130 V delivered from an Owl™ EC 300 XL power supply (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... NSC media was switched to “D3” differentiation media (DMEM/F12 Gibco #11330032; 0.5X B27+ supplement Gibco #17504-044 ...
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bioRxiv - Neuroscience 2020Quote: ... All cultureware used for hCMEC/D3 cells was coated with rat tail collagen type I (Gibco) diluted in phosphate buffered saline (PBS ...
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bioRxiv - Physiology 2024Quote: ... Transfection of hCMEC/D3 with the miRNA mimics was achieved using Lipofectamine RNAiMAX transfection reagent (ThermoFisher). Then ...
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bioRxiv - Cell Biology 2020Quote: ... The mouse macrophage cell line RAW264.7 clone D3 was cultured in DMEM containing L-glutamine (Life Technologies), 10% heat-inactivated FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... Murine ESCs (D3) and murine iPSC lines were maintained on mitomycin-treated MEFs in DMEM glutamax (Gibco) that contained 15% fetal bovine serum (Eurobio) ...
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bioRxiv - Cell Biology 2021Quote: ... D3 was cultured in DMEM/F12 (3:1) medium supplemented with 10% fetal bovine serum (Thermo Fisher Scientific), 8 ng/mL Cholera Toxin (CELL technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... 3000 Plp1+ cells from each group (control, D1, D3, D7) were collected into 96-well plates (Life Technologies) coated with BSA (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... NSC media was switched to “D3” differentiation media (DMEM/F12 Gibco #11330032; 0.5X B27+ supplement Gibco #17504-044, 1X N2 supplement Gibco #17502-048 ...
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bioRxiv - Neuroscience 2020Quote: ... hCMEC/D3s were washed three times with room temperature (RT) PBS and lysed using Pierce RIPA buffer (Thermo Scientific) containing 1x Halt protease inhibitor cocktail (Thermo Scientific ...
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bioRxiv - Systems Biology 2022Quote: ... at a density of 0.25 x 106 cells/cm2 and cultured for three (d3) or six (d6) days in DMEM/F12 (Gibco) containing 10% FBS and 0.05 mg/ml gentamycin (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... D3 cells were cultured in DMEM/F12 (3:1) medium supplemented with 10% fetal bovine serum (Thermo Fisher Scientific), 8 ng/mL Cholera Toxin (CELL technologies) ...
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bioRxiv - Cell Biology 2024Quote: ... The hcMEC / D3 cells were transfected with HBA1 RNAi plasmid mixed with LipofectamineTM 3000 and P3000TM (Invitrogen / Thermo Fisher) in advance for 24 hours ...
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bioRxiv - Cell Biology 2024Quote: ... The hcMEC / D3 cells were transfected with HBA1 RNAi plasmid mixed with LipofectamineTM 3000 and P3000TM (Invitrogen / Thermo Fisher) in advance for 24 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Both the LentiCRISPRv2-sgRNA plasmid and the ss donor oligonucleotides were transfected into D3 mouse ESC line using Lipofectamine 3000 (Invitrogen). Transfected cells were selected with puromycin (1.5 μg/ml ...
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bioRxiv - Biochemistry 2021Quote: ... Both the LentiCRISPRv2-sgRNA plasmid and the ss donor oligonucleotides were transfected into D3 mouse ES cell line using lipofectamine 3000 (Invitrogen). Transfected cells were selected with puromycin (1.5 μg/ml ...
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bioRxiv - Physiology 2024Quote: Cells from the human brain microvascular EC line hCMEC/D3 [37] were seeded on collagen I-coated 35 mm tissue culture dishes (Nunc) and grown in EGM2-MV (Lonza).
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bioRxiv - Neuroscience 2024Quote: ... n=15 full larvae) or extracted from hCMEC/D3 cells seeded in 12 well/plates using a standard TRIzol (Invitrogen) method ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Both of the in vitro systems depend on the application of three-dimensional embryoid body cultures derived from either mouse embryonic stem cells (mESC, ES-D3, ATCC, CRL-1934) or human episomal induced pluripotent stem cells (hiPSC, Gibco, A18945). The teratogenicity determination of the mEST is based on the half maximal inhibition of differentiation into beating cardiomyocytes (ID50 ...
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bioRxiv - Neuroscience 2021Quote: ... or with a pool of two different USP30 siRNAs (D1: 5’-CAAAUUACCTGCCGCACAA-3’; D3, 5’-ACAGGAUGCUCACGAAUUA-3’, Dharmacon; siUSP30) by using Lipofectamine RNAiMAX (Thermo Scientific) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: Clathrin and caveolin-1 knockdown in polarized hCMEC/D3 cell monolayers was performed using siRNA kit containing RNAi mix (Invitrogen, CA) and reduced serum medium ...
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bioRxiv - Neuroscience 2022Quote: ... GFP and non-GFP K44-negative-dominant dynamin transfections of hCMEC/D3 cell monolayers were performed using Lipofectamine reagent (Invitrogen, CA) as per manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... The nuclear pellet was washed with 1X wash buffer C and then once with shearing buffer D3 with Halt protease inhibitor cocktail (Thermo scientific)] ...
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bioRxiv - Developmental Biology 2024Quote: ... NMP-L cells were replated at end of D3 and cultured for 8 to 9 days and transfected using Lipofectamine 3000 kit (Thermo Fisher) following manufacturer’s instructions with a combination of piggyBac plasmids (pm-eGFP + HypBase or pm-mKate +H2B-mEos3.2 + HypBase ...
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bioRxiv - Genetics 2024Quote: ... The reactions were then loaded onto a 1% agarose gel and electrophoresed in an OwlTM D3-14 electrophoresis box (Thermo Scientific, MA, USA) containing 1X TBE buffer for 30 min at 180 V delivered from an OwlTM EC 300 XL power supply (Thermo Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The reactions were then loaded onto a 1% agarose gel and electrophoresed in an Owl™ D3-14 electrophoresis box (Thermo Scientific, MA, USA) containing 1X TBE buffer for 30 min at 180 V delivered from an Owl™ EC 300 XL power supply (Thermo Scientific ...