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Citations for Thermo Fisher :
1 - 29 of 29 citations for Complement anaphylatoxin C5a since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... assays were run using the Complement C5a Human ELISA Kit (Invitrogen). For sample preparation ...
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bioRxiv - Biochemistry 2023Quote: ... C3a and C5a constructs were all cloned into pFastbac1 vector (ThermoFisher) and expressed in Sf9 insect cells as secreted proteins using baculovirus infection system ...
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Cryptococcus neoformans capsule regrowth experiments reveal dynamics of enlargement and architecturebioRxiv - Microbiology 2021Quote: ... or 20% complement (Fisher Scientific #642831) and placed on the slide portion of the dish ...
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bioRxiv - Pathology 2020Quote: ... complement volume with Nuclease-Free Water (Thermofisher). PCR reaction conditions ...
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bioRxiv - Immunology 2020Quote: ... C5a was removed using a spin column with 30 kDa cut-off (Thermo Fisher).
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bioRxiv - Neuroscience 2019Quote: ... and complement factor D (ThermoFisher-Invitrogen, LaFayette, CO). The mean value for all determinations of CD81 in each assay group was set at 1.00 ...
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bioRxiv - Neuroscience 2019Quote: ... and complement factor D (ThermoFisher-Invitrogen, LaFayette, CO). The mean value for all determinations of CD81 in each assay group was set at 1.00 ...
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bioRxiv - Immunology 2022Quote: ... and transfected into E1-complement HEK293A cells (ThermoFisher, R705-07) for the adenovirus packaging and generation ...
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Cryptococcus neoformans capsule regrowth experiments reveal dynamics of enlargement and architecturebioRxiv - Microbiology 2021Quote: ... or 1:50 goat anti-Complement C3 polyclonal—FITC (Invitrogen). H99 cells with either fluorescently conjugated anti-IgM mAb ...
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bioRxiv - Microbiology 2022Quote: ... we used the following antibodies: α-complement C3 (primary: mAb 11H9, ThermoFisher catalog no ...
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bioRxiv - Molecular Biology 2022Quote: ... in which the FBS was replaced with 2% B27 complement (Life Technologies).
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bioRxiv - Molecular Biology 2024Quote: Complement DNA (cDNA) synthesis was performed using TaqMan® Reverse Transcription Reagents (Life Technologies) and analyzed by quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Immunology 2021Quote: ... and stained with an anti-Guinea Pig Complement C3 FITC (polyclonal, ThermoFisher Scientific, Waltham, MA). Cells were then fixed with 4% formaldehyde solution and fluorescence was evaluated on a LSRII flow cytometer (BD Bioscience ...
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bioRxiv - Cell Biology 2021Quote: ... 10% fetal bovine serum complement deactivated at 56°C for 30 minutes (FBS; Gibco, 11573397;) and incubated at 27°C ...
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bioRxiv - Microbiology 2022Quote: ... and stained with an anti-guinea pig complement C3 FITC (polyclonal, ThermoFisher Scientific, Waltham, MA). Cells were fixed with 4% formaldehyde solution and fluorescence was evaluated on a LSRII (BD Bioscience ...
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bioRxiv - Neuroscience 2023Quote: ... Complement C1q A Chain (C1qa) and glyceraldehyde-3-phosphate dehydrogenase (Gapdh) as endogenous control (Life Technologies). Each sample was run in triplicate in 20 μl of reaction volume using TaqMan Universal PCR Master Mix according to the manufacturer’s instructions (Life Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... an 18-mer DNA oligonucleotide containing the ETV5 promoter (GGTTATGAATGAAAAACC) and its reverse complement were purchased from ThermoFisher with HPLC purification ...
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bioRxiv - Microbiology 2021Quote: Complement-labelled bacteria (~5 × 107 bacteria/ml) were incubated with 2.5 μM of Sytox Blue Dead Cell stain (Thermofisher). Samples were diluted to ~2.5 × 106 bacteria/ml in RPMI-HSA and subsequently analyzed on a MACSquant VYB (Miltenyi Biotech ...
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bioRxiv - Biophysics 2019Quote: ... were all purchased from Bio-Rad Laboratories (Hercules, CA, USA). Complement C3b monoclonal antibody (catalog no. MA1-40155, lot no. SH2428445) was purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Immunology 2022Quote: ... Deposition of immunoglobulin (IgG) and complement component 3 (C3) in the kidney tissue were stained with PE labeled goat anti-mouse IgG (Invitrogen) and FITC labeled rat anti-mouse C3 (Cedarlane ...
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Sex and species associated differences in Complement-mediated immunity in Humans and Rhesus macaquesbioRxiv - Immunology 2023Quote: ... Complement-mediated lysis was measured by staining with live/ dead fixable aqua dead cell marker (Life Technologies, Catalogue no. L34957) prior to fixation ...
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bioRxiv - Genomics 2020Quote: ... Both the template strand and its reverse complement were labeled using the Pierce Biotin 3’ End Labeling Kit (Thermo Fisher Scientific) and the complementary oligonucleotides were annealed ...
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bioRxiv - Microbiology 2020Quote: ... the plasmid complement of each isolated clone was determined using primer pairs previously described (77) and DreamTaq Green DNA Polymerase (ThermoFisher Scientific). Each clone was confirmed to contain all B31-specific plasmids ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated O/N at 4°C with gentle agitation with 1:2000 goat anti-human complement C3 antibody (Thermo Scientific) diluted in 1X TBS/0,5% Tween/3% BSA ...
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bioRxiv - Genomics 2023Quote: ... The sections were blocked with anti-donkey serum 5% and labelled with anti-Complement C3 (Catalog #PA1-29715 from Life Technologies/ThermoFisher) and TNFRSF1B (Catalog #MA5-31661 from Life Technologies/ThermoFisher) ...
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bioRxiv - Biophysics 2022Quote: ... The cell culture medium (RPMI 1640) and complements (10% FBS, 1% Hepes 1M, 1% Glutamax, 1% Pen/Strep) were obtained from Gibco (Life technologies). Cells were monthly tested for the presence of mycoplasma.
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bioRxiv - Genomics 2023Quote: ... The sections were blocked with anti-donkey serum 5% and labelled with anti-Complement C3 (Catalog #PA1-29715 from Life Technologies/ThermoFisher) and TNFRSF1B (Catalog #MA5-31661 from Life Technologies/ThermoFisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... and their reversed complement using the Sequest algorithm (Ver28) embedded in an in-house-developed software suite88 or Proteome Discoverer™ Software (Thermo Fisher Scientific). MS1 mass tolerance was set to be 50 ppm ...
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bioRxiv - Microbiology 2022Quote: ... we used the following antibodies: α-complement C3 (primary: mAb 11H9, ThermoFisher catalog no. MA1-40046; secondary: Mouse anti-Rat IgG2a, FITC, Thermofisher catalog no. 11-4817-82), α-surfactant protein D (primary ...