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Citations for Thermo Fisher :
1 - 45 of 45 citations for Bordetella pertussis Filamentous Hemagglutinin FHA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Pertussis toxin (Invitrogen, cat# PHZ1174) was prepared as a 100 ng/mL solution in water ...
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bioRxiv - Physiology 2019Quote: ... for filamentous actin staining and TOPRO3® (Thermofisher) for nuclei staining ...
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bioRxiv - Microbiology 2021Quote: ... plenti6 FHA-V5 vector was modified from plenti6-V5 gateway vector (Thermo Fisher scientific V49610) by Gibson cloning ...
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bioRxiv - Microbiology 2019Quote: ... Filamentous actin was stained with fluorescently conjugated phalloidin (Invitrogen, USA) according to the manufacturer protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... Filamentous Actin was stained with Phalloidin-Rhodamine (Molecular Probes, R415).
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bioRxiv - Microbiology 2023Quote: ... Pertussis toxin (PTx) was purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... or mouse monoclonal antibody against hemagglutinin (Thermo Scientific cat # 26183) (1:5000 dilution) ...
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bioRxiv - Cell Biology 2022Quote: ... Filamentous actin was labeled with Oregon Green 488 Phalloidin (Cat# O7466, Invitrogen) or alernatively Rhodamine Phalloidin (Cat# R415 ...
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bioRxiv - Cell Biology 2023Quote: ... Filamentous actin was stained with Alexa 647-conjugated phalloidin (1:500; Invitrogen). Embryos were mounted in ProLong Gold (Molecular Probes ...
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bioRxiv - Cancer Biology 2022Quote: ... filamentous actin and nuclei were stained using Alexa Fluor™ 488 Phalloidin (Invitrogen) and Hoechst 33342 (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... Filamentous (F)-actin was labelled with directly conjugated Alexa Fluor 568 Phalloidin (ThermoFisher Scientific). The DNA-binding dye Hoescht 33258 (Molecular Probes ...
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bioRxiv - Developmental Biology 2020Quote: ... Filamentous actin was detected using phalloidin (1:50; Thermo Fisher Scientific, Walham, MA; #A12379). Labeling with WGA-555 (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... Filamentous actin (F-actin) was stained using 0.1 mM rhodamine-conjugated phalloidin (Molecular Probes). TUNEL was performed using the In Situ Cell Death Detection Kit (Roche).
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bioRxiv - Developmental Biology 2020Quote: ... Rhodamine phalloidin (Phalloidin 555) was used to visualize filamentous actin (1:300, Molecular Probes). Preparations were mounted with ProLong Gold with DAPI (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... and pertussis toxin (600 ng/ml final concentration, Invitrogen Cat# PHZ1174). Latrunculin-A and IPA-3 were reconstituted in Dimethyl Sulfoxide (DMSO) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and filamentous actin was labeled using AlexaFluor 488-conjugated phalloidin (1:25, Molecular Probes A12379). For in situ hybridization ...
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bioRxiv - Cell Biology 2022Quote: ... Filamentous actin (F-actin) was labelled using phalloidin Alexa-Fluor 568 (1:200, Life Technologies). All antibodies used in this study have previously been extensively used and validated for immunofluorescence studies.
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bioRxiv - Bioengineering 2022Quote: ... To visualize filamentous actin Alexa Fluor 647 conjugated phalloidin was used (Invitrogen, dilution 1:4000). Hoechst was used to visualize nuclei ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Where appropriate cells were treated with pertussis toxin (PTX, Thermo Fisher, UK) at a range concentration of 2 pg/ml to 200 ng/ml or cholera toxin (CTX ...
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bioRxiv - Neuroscience 2023Quote: ... mice were injected with 200 ng of pertussis toxin intraperitoneally (PHZ1174, Thermofisher). EAE clinical signs and weight were monitored daily from 7 days post-immunisation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... before labeling filamentous actin and nuclei with AlexaFluor 488-conjugated phalloidin (1:25, Molecular Probes A12379) and the fluorescent dye 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Microbiology 2023Quote: ... Strep-tag (NBP2-41073; Novusbio) or influenza virus hemagglutinin tag (2-2.2.14; Thermo Scientific). After washing with PBS with 1% BSA ...
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Volumetric Compression Shifts Rho GTPase Balance and Induces Mechanobiological Cell State TransitionbioRxiv - Biophysics 2023Quote: ... and a dye-conjugated antibody anti-hemagglutinin (HA) tag-Dylight 488 (26183-D488, Invitrogen). The secondary antibodies used in this study are ...
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bioRxiv - Neuroscience 2020Quote: ... the mice were injected with 200ng pertussis toxin (Thermo Fisher Scientific;Beijing, China). After 48h ...
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bioRxiv - Molecular Biology 2024Quote: ... T cells were treated with either 20nM Pertussis Toxin (PHZ1174, Gibco Life Technologies) or 1µM AMD-3100 (S8030 ...
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bioRxiv - Molecular Biology 2024Quote: ... T cells were treated with either 20nM Pertussis Toxin (PHZ1174, Gibco Life Technologies) or 1µM AMD-3100 (S8030 ...
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bioRxiv - Cell Biology 2021Quote: Mouse hemagglutinin (HA) tagged Lphn2 construct was synthesized by GeneArt Gene Synthesis (Thermo Fisher Scientific) and cloned into the Gateway system pENTR221 vector (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... Filamentous actin (F-actin) was visualized using phalloidin conjugated with Alexa Fluor 488 (Cat# A12379, Invitrogen/Thermo Scientific). Globular monomeric actin (G-actin ...
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bioRxiv - Cell Biology 2019Quote: ... Filamentous actin (F-actin) was visualized using phalloidin conjugated with Alexa Fluor 488 (Cat# A12379, Invitrogen/Thermo Scientific). Globular monomeric actin (G-actin ...
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bioRxiv - Developmental Biology 2022Quote: ... Filamentous actin was stained with phalloidin conjugated to Alexa Fluor® 594 or 647 (1:1000; Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... Filamentous actin (f-actin) was stained using 1 U per construct of Alexa Fluor 488 Phalloidin (Invitrogen A12379) in HBSS for 1 hour ...
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bioRxiv - Zoology 2022Quote: ... and filamentous actin (F-actin) was stained using Alexa Fluor® 546 Phalloidin (Thermo Fisher Scientific, Waltham, MA, USA). Microscopic observations were performed using an Olympus BX53 microscope ...
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bioRxiv - Cell Biology 2019Quote: ... Filamentous actin was visualized with Alexa fluor 488- or Alexa fluor 594 -phalloidin (for 30 min, Invitrogen, Molecular probes).
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bioRxiv - Cell Biology 2019Quote: ... Filamentous actin was visualized with Alexa fluor 488- or Alexa fluor 594 -phalloidin (for 30 min, Invitrogen, Molecular probes).
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bioRxiv - Microbiology 2022Quote: ... to label filamentous actin followed by DAPI nucleic acid stain (DAPI dihydrochloride, 300 nM in PBS, Invitrogen, United Kingdom). Filter membranes were washed then mounted with ProLong Glass antifade mountant (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Cells were incubated with antibody against anti-hemagglutinin mouse IgG monoclonal conjugate Alexa Fluor 488 conjugated (Invitrogen) for 1h ...
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bioRxiv - Microbiology 2021Quote: The storage medium of the filamentous bacterial fraction was replaced by Dulbecco’s Phosphate Buffered Saline liquid (DPBS, Thermo Fisher Scientific) with 1.5% ultra-low gelling temperature agarose A5030 (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2019Quote: ... GenBank accession number BC057894.1) with an N-terminal hemagglutinin (HA) tag were reverse transcribed with SuperScript III Reverse Transcriptase (Invitrogen) and PCR-amplified using Phusion high-fidelity DNA polymerase (New England Biolabs ...
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bioRxiv - Immunology 2020Quote: ... and 1µg/ml biotinylated recombinant hemagglutinin (rHA) (Whittle et al., 2014) conjugated to streptavidin APC (cat. no: S868, Invitrogen). To exclude dead cells ...
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bioRxiv - Cell Biology 2020Quote: ... Filamentous actin was labeled with Alexa Fluor®647 Phalloidin and Alexa Fluor®488 (Invitrogen, catalog #A22287, and #A12379, 1:200), and cell nuclei were labeled with DAPI (catalog #D9542 ...
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bioRxiv - Cancer Biology 2022Quote: ... Nuclei were counterstained with DAPI (4’,6-diamidino-2-phenylindole) and filamentous actin with Alexa Fluor 488-conjugated phalloidin (Thermo Fisher Scientific).
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bioRxiv - Immunology 2022Quote: ... MBP peptide NPVVHFFKNIVTPR and influenza hemagglutinin PRFVKQNTLRLAT peptide were labeled with Alexa Fluor 488 (Alexa488) tetrafluorophenyl ester (Invitrogen, Carlsbad, CA) and used as probe peptides for DP4.1 ...
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bioRxiv - Microbiology 2020Quote: ... Gene specific primers with cloning sites for H3N2 neuraminidase or hemagglutinin were used to create cDNA via a one-step RT-PCR reaction (SuperScript III-Platinum Taq mix, ThermoFisher Scientific). The cDNA products were cut with appropriate restriction enzymes ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant neuraminidase and recombinant hemagglutinin were biotinylated in a 20:1 molar ratio of biotin to antigen with EZ-Link NHS-Biotin (ThermoFisher, 20217) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... Hooke Laboratories adjusted concentrations by lot) followed by the intraperitoneal injection of pertussis toxin (Hooke Laboratories, included in the induction kits) 1x PBS (Gibco, 10010023) on day 0 and day 1 (200-225 ng per animal ...