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Citations for Thermo Fisher :
3501 - 3550 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 2 µg DNA was mixed with 6 µL of Fugene HD in 100 µL of Opti-MEM (Thermo Fisher Scientific) and incubated at room temperature for 30 min ...
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bioRxiv - Biophysics 2022Quote: ... Purified Syb2 (29–96, S61C) was labeled with 6× molar excess BODIPY FL N-(2-aminoethyl)-maleimide (BDPY) (Molecular Probes) and SN25 FL (1–206 ...
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bioRxiv - Molecular Biology 2022Quote: ... Staining of nuclei with 4ʹ,6-diamidino-2-phenylindole (DAPI) was performed with mounting solution (ProLong Diamond Antifade Mountant with DAPI (ThermoFisher)) or 50% glycerol in 1×PBS.
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bioRxiv - Biophysics 2022Quote: ... Fractions containing S15 were consolidated and concentrated to ∼2 mg/mL using a 6 mL 3K molecular weight cutoff centrifugal concentrator (ThermoFisher), before being aliquoted and flash-frozen in liquid nitrogen for long-term storage at -80°C.
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bioRxiv - Neuroscience 2022Quote: ... CHO cells were plated at 1 x 106 cells/well in a 6-well plate and cultured for 24 hr in 2 ml of F12 medium (Gibco) supplemented with 10% FBS and 1% Glutamax (Gibco ...
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bioRxiv - Biochemistry 2023Quote: Doxycycline-inducible C2C12 cells were seeded for most treatments at 0.1X106 cells per well in 6-well plates with 2 ml of DMEM [high glucose DMEM with pyruvate (Gibco, 12800), 3.7 g/L NaHCO3 ...
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bioRxiv - Biophysics 2023Quote: ... Adherent semiconfluent HEK293SG cells cultured in DMEM:F12 medium supplemented with 10% FBS were transfected in 6-well plates with 2 μg/well of plasmid DNA complexed with Lipofectamine 2000 reagent (Invitrogen). The cells were incubated for 48 hrs at 37 °C under 7% CO2 ...
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bioRxiv - Plant Biology 2023Quote: ... Protein pellets were resuspended in urea buffer (6 M urea, 2 M thiourea, 100 mM ammonium bicarbonate, pH 8.0) and quantified using EZQ (Invitrogen/Life Technologies).
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bioRxiv - Cell Biology 2023Quote: The ROS dye assay was performed using Di(Acetoxymethyl Ester) (6-Carboxy-2’,7’-Dichlorodihydrofluorescein Diacetate) ROS dye (Invitrogen, D23844), a fluorogenic dye that is converted to 6-CarboxyFluorescein ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells at ∼50% confluency in 6-well plates were incubated with 2 mL Opti-MEM-I medium (Gibco, 31985062) supplied with 6 µL Lipofectamine 2000 transfection reagent (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Cells were transfected in 6-well plates with 2 µg of the indicated constructs in the presence of Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µg DNA was mixed with 6 µL of FuGENE HD in 100 µL of Opti-MEM (Thermo Fisher Scientific) and incubated at RT for 10 min before being added to HeLa cells grown in 35-mm glass-bottomed dishes (AGC Technology Solutions ...
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bioRxiv - Neuroscience 2024Quote: ... with real time quantitative gene expression determined using using Taqman probles (Table 2) and analysed by the Quant Studio 6 Flex Real-Time PCR System (Invitrogen).
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bioRxiv - Neuroscience 2024Quote: ... The cells were washed 6 times with PBS for 2 min and incubated with Alexa Fluor 568- (1:500 dilution, Invitrogen), Alexa Fluor 488- (1:500 dilution ...
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bioRxiv - Bioengineering 2024Quote: ... Hepatic endoderm specification was initiated on differentiation days 6 ─ 10 using RMPI supplemented with 2% B27 and insulin (ThermoFisher, #17504044), 10ng/ml FGF-2 ...
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bioRxiv - Microbiology 2024Quote: ... 1,000,000 EGI-1 or HepG2 cells were plated in 2 mL complete DMEM media in 6-well Nunclon Delta-treated plates (ThermoFisher) and incubated at 37 °C with 5% CO2 overnight ...
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bioRxiv - Bioengineering 2024Quote: ... Cell nuclei and actin cytoskeleton were stained using 4ʹ,6-diamidino-2-phenylindole dihydrochloride (DAPI; D1306, Invitrogen, Thermo Fisher Scientific) and Alexa Fluor 633 phalloidin (A22284 ...
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bioRxiv - Bioengineering 2024Quote: ... Cell nuclei and actin cytoskeleton were stained using 4ʹ,6-diamidino-2-phenylindole dihydrochloride (DAPI; D1306, Invitrogen, Thermo Fisher Scientific) and Alexa Fluor 633 phalloidin (A22284 ...
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bioRxiv - Cancer Biology 2024Quote: IMR-5/75 cells were plated in duplicates on a 6-well plate in 2 mL complete RPMI phenol red-free media (Gibco). One day after seeding ...
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bioRxiv - Genetics 2024Quote: 293T cells were seeded into 48- or 6-well plates and transfected respectively with 2 µL or 7.5 µL of Lipofectamine 3000 (ThermoFisher Scientific) using 750 ng or 3 µg of either ABE-GFP or BE4-GFP base editor-expressing plasmid ...
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bioRxiv - Cell Biology 2020Quote: ... BN-PAGE was performed using NativePAGE 3-12% or 4-16% Bis-Tris gels and associated running buffer (Invitrogen). The cathode buffer was supplemented with 0.002% (w/v ...
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bioRxiv - Immunology 2021Quote: ... Excess liquid was blotted for 3 seconds with a force of -4 using a Vitrobot Mark IV (Thermo Fisher) and plunge frozen into liquid ethane ...
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bioRxiv - Neuroscience 2020Quote: ... third instar larvae were dissected and stimulated with a HL-3 solution of 90 mM K+/0.5 mM Ca2+/4 μM fixable FM1-43 (Invitrogen) for 1 min ...
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bioRxiv - Genomics 2020Quote: ... Two TaqMan probes targeting the MLLT3 exon 3-4 and exon 9-10 splice junctions (ThermoFisher, Hs00971092_m1 and Hs00971099_m1) were used with cDNA diluted 1:5 in dH2O to perform qPCR for six replicates per sample on an Applied Biosystems Quant Studio 6 Flex ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were loaded with culture medium containing 3 μM of the fluorescent calcium indicator Fluo-4/AM (Life Technologies) and Pluronic F-127 (0.2% w/v ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibody incubation was performed for 3-4 days and DiD (Thermo Fisher Scientific-Molecular Probes L7781; 1µg/ml) labeling was added from the second day to show the whole brain morphology ...
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bioRxiv - Synthetic Biology 2021Quote: N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (NBD-PE) was supplied by Thermo Fisher Scientific Inc ...
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bioRxiv - Microbiology 2021Quote: ... Cell monolayers were then stained with 3 mL of overlay containing a 1:1 mixture of 1.2% oxoid agar with 4% neutral red (Gibco) and 2X DMEM with 2% (vol/vol ...
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bioRxiv - Microbiology 2022Quote: ... incubated for 10 min in the dark at room temperature with FM 4-64FX (3 μg ml-1) (ThermoFisher). Cells were applied to a 5% agarose pad containing M9 minimal media with glucose (0.4%) ...
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bioRxiv - Cancer Biology 2022Quote: ... RT reactions were performed with 3-4 µg total RNA using oligo-dT and SuperScript III RT (ThermoFisher Scientific). QPCR was performed as previously described [20] ...
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bioRxiv - Genetics 2022Quote: Immunoprecipitations were performed using 1 μg of Anti-Trimethyl-Histone-3-Lys-4 (-Thermo Fisher Scientific; catalog# PA5-17420) overnight at 4 ◦ C ...
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bioRxiv - Molecular Biology 2020Quote: ... heated at 95°C for 3 min and seprated on a Novex WedgeWell 4-12% Tris-Glycine Gel (Invitrogen). Proteins were transferred to an PVDF Transfer Membrane (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... After 3 days the overlay was removed and cell monolayers were fixed with 4% formaldehyde (28906, Thermo Fisher Scientific) at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Uptake of fatty acids was quantified after incubation with 1uM 4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic acid (Bodipy-FL C16, Thermo Fisher) at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... or 5 µM BODIPY™ FL C5-Ceramide (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosine) (Invitrogen, D3521) together with 5 µM delipidated BSA (as a delivery carrier ...
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bioRxiv - Cell Biology 2021Quote: ... WT and SIRT1 KO mESCs cultured in serum-free ESGRO medium or serum-containing M10 medium were incubated with 5 µM BODIPY™ FL C5-Sphingomyelin (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosyl Phosphocholine) (Invitrogen, D3522) or 5 µM BODIPY™ FL C5-Ceramide (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosine ...
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bioRxiv - Neuroscience 2022Quote: 100 to 150 3-dpf zebrafish larvae were bathed in Yo-Pro-1 (4 µM, 30 min) (Invitrogen, Y3603), then washed 3 times in blue water to label lateral line hair cells ...
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bioRxiv - Neuroscience 2022Quote: ... The craniotomy was then sealed with two glass coverslips (3 mm and 4 mm diameter, Thermo Fisher Scientific, UK) glued to one another using optical adhesive (Norland ...
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bioRxiv - Physiology 2022Quote: ... Cells were fixed with ice-cold 4% paraformaldehyde solution and cell nuclei were stained with To-Pro-3 (Invitrogen). Samples were mounted with Mowiol (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: BODIPY FL C16 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic Acid, Invitrogen D3821) was used to assess the ability of 3T3s to transport fatty acids into the cell from their surroundings ...
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bioRxiv - Biochemistry 2023Quote: ... SDS-PAGE was carried out on either NuPAGE 4-12% Bis-Tris or 3-8% Tris-Acetate gels (Invitrogen). Western blotting analysis was carried out according to standard protocols with the antibodies listed in Table S5.
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bioRxiv - Biochemistry 2022Quote: ... Acly-/- MEFs were maintained at <80% confluence (passaged every 3-4 days) in high glucose DMEM (Gibco #11965-084) with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... cells at passage 3-4 were seeded in to black walled clear bottomed 96 well plates (Pierce, ThermoFisher Scientific) at a density of 7,500 cells per well ...
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bioRxiv - Immunology 2023Quote: ... and packaging vector psPAX2 were mixed in a 4:3:1 ratio in OPTI-MEM (Thermo Fisher Scientific, 31985070) and PEI (Polysciences ...
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bioRxiv - Biochemistry 2023Quote: ... BODIPY-CE (cholesteryl 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3- dodecanoate) was obtained from Life Technologies. Lipids (with the exception of NBD-LPC ...
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bioRxiv - Biochemistry 2023Quote: ... incubated for 30 min at 4 °C and analyzed on 3-12% gradient Bis-Tris native gels (Life Technologies) at 4 °C ...
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bioRxiv - Genomics 2023Quote: ... 3-4 days post-nucleofection (depending on cell confluency) selection was started using 400 ng/mL Puromycin (ThermoFisher A1113802) in FM (PFM) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were passaged every 3-4 days during the log phase of growth with Trypsin/EDTA solution (Gibco, R001100) following a PBS wash ...
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bioRxiv - Neuroscience 2024Quote: ... Mouse cochleae were fixed in 4% PFA for 3 hours and microdissected in cold 20% RNAlater (Thermo Fisher Scientific) in PBS ...
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bioRxiv - Immunology 2024Quote: ... The transduced cells were further cultured for 3 days under antibiotic selection pressure (4 μg/mL puromycin (Thermo Fisher)) ...