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Citations for Thermo Fisher :
3451 - 3500 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... the skin samples were washed 2-3 times with buffer saline (1X PBS; Gibco, Thermo Fisher Scientific). The adipose tissue and associated blood vessels were removed ...
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bioRxiv - Genomics 2020Quote: ... the skin samples were washed 2-3 times with buffer saline (1X PBS; Gibco, Thermo Fisher Scientific). The adipose tissue and associated blood vessels were removed ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were routinely passaged after 2-3 days or after reaching ∼80 % confluency using TrypLE Express (Gibco) for the detachment of cells from culture flasks ...
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bioRxiv - Immunology 2022Quote: ... 2 μm paraffin-embedded kidney sections were incubated with an anti-cleaved caspase-3 antibody (ThermoFisher Scientific). After incubation with goat anti-rabbit-HRP secondary antibody (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... in the reverse phase using a preconcentration column (75 μm DI × 2 cm, 3 μm, Thermo Scientific) and an analytical column (Acclaim PepMap C18 ...
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bioRxiv - Genetics 2022Quote: ... Cells were passaged at 70-80% confluence every 2-3 days using Trypsin 0.25% EDTA (Life Technologies). mESCs were plated at a density of 1 x 105 cells/ml ...
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bioRxiv - Microbiology 2022Quote: PBMCs were thawed according to standard procedure and rested 2-3 h in IMDM (Gibco/ThermoFisher Scientific) containing 5% human AB serum (SAB ...
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bioRxiv - Microbiology 2022Quote: PBMCs were thawed according to standard procedure and rested 2-3 h in IMDM (Gibco/ThermoFisher Scientific) containing 5% human AB serum (SAB ...
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bioRxiv - Microbiology 2022Quote: Anti-ORF8 mAb (#1-3-2) was coupled to aldehyde/sulfate latex beads (Thermo Fisher Scientific A37304). The antibody was mixed with the latex beads and shaken at room temperature overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated for 2–3 minutes (mins) at room temperature in 0.05% (v/v) trypsin (Gibco 25300-054), and tapped to release ...
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bioRxiv - Molecular Biology 2023Quote: ... An Acclaim PepMap 100 trap column (75 μm × 2 cm, C18, 3 μm, 100 Å, Thermo Scientific) was used together with an analytical PepMap RSLC C18 column (150 μm × 15 cm ...
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bioRxiv - Cell Biology 2023Quote: ... after the first fast centrifugation step platelets were incubated with 3 µM Fura-2 AM (#F1221, Invitrogen) and 0.2% Pluronic F-127 (#P3000MP ...
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bioRxiv - Biochemistry 2023Quote: ... cells were incubated with 1.2mM 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT, Thermo Fisher Scientific) solution ...
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bioRxiv - Systems Biology 2023Quote: Total RNAs (2–3 μg) were extracted from tobacco leaves using PureLink Plant RNA Reagent (Invitrogen, #12322012) and used for cDNA synthesis with M-MLV Reverse Transcriptase (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... The supernatant was collected on day 3 and analyzed by human IL-2 ELISA Kit (Thermo Fisher) according to the manufacturer’s protocol.
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bioRxiv - Genomics 2024Quote: ... A 2-3 cm2 skin biopsy harvested and transferred into NMR fibroblast medium: alphaMEM (Gibco™ 12571063) containing 15% FBS (Cytiva ...
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bioRxiv - Cancer Biology 2024Quote: Proliferation assays were performed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Thermo Fisher Scientific) assay ...
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bioRxiv - Immunology 2024Quote: Jurkat cells were loaded with 3 µM Fura-2 AM and 0.05% Pluronic®F-127 (Invitrogen) in imaging buffer ...
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bioRxiv - Cell Biology 2024Quote: ... or F-12K medium (Caisson; Hela-S3 and PC-3) supplemented with 2 mM L-glutamine (Gibco), 100 U/mL penicillin ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of plasmid was used to transfect 750,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5 μL; Thermo Fisher Scientific) in OptiMEM (400 μL ...
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bioRxiv - Molecular Biology 2020Quote: DR-U2OS and -HCT116 cells and EJ-U2OS and -HCT116 reporter cells were plated in 6 well plates and transfected with 5 μL Lipofectamine RNAiMAX transfection reagent (Thermo Fisher) and siRNAs (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: Spinal cord tissue from stage HH 25-26 (Day 5-6) chick embryos (Venkateshwara hatcheries Ltd.) were dissected out in L-15 medium (Gibco, HiMedia) with added 1x penstrep (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... HAP1 cells with a low passage number were seeded into 6 well microtiter plates at a density of 5 × 105 cells/well in IMDM medium (#21980032, ThermoFisher Scientific) containing 10 % FBS (Sigma Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... The medium was changed every day and cells were passaged every 5-6 days using PBS without Calcium and Magnesium (Life Technologies).
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bioRxiv - Biochemistry 2022Quote: ... 5 μl of each binding reaction was loaded directly onto a pre-run 6% acrylamide DNA retardation gel (Thermo Fisher Scientific) and electrophoresed at ≤20 V cm−1 at 4 °C ...
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bioRxiv - Genomics 2022Quote: ... 2.6 μL nuclease-free water and 5 μL cDNA template on the ABI Prism 7500 Fast Sequence Detection System (Life Technologies). Thermo-cycling conditions were as follows ...
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bioRxiv - Cancer Biology 2021Quote: ... The fluorescent reaction was measured every 40 sec for 8 min and subsequent every 5 min for 6 times using a microplate reader (Thermo Scientific) with enzyme kinetics model ...
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bioRxiv - Molecular Biology 2022Quote: ... were transfected using Lipofectamine 3000 kit (7.5 µl Lipo 3000 and 5 µl P3000 reagents per 6-well; Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2022Quote: ICabs A4 and B7 were labelled with NHS-Rhodamine (5/6-carboxy-tetramethyl-rhodamine succinimidyl ester, ThermoFisher Scientific (catalogue no. 46406)) as described by the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... 5×105 cells were seeded in 6 cm plates and transfected with siRNAs using Lipofectamine™ RNAiMAX Transfection Reagent (#13778030, Invitrogen) as per manufacturer protocol ...
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bioRxiv - Genetics 2022Quote: ... Quantitative expression analysis of Nos1ap was performed using mouse-specific TaqMan Gene Expression assay (Mm01290688_m1; mapping to exons 5 and 6) (Applied Biosystems, MA). Real-time qPCR was performed on a 7900HT Fast Real-Time PCR System or QuantStudio 5 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genetics 2022Quote: ... ~1 × 105 hTSC were seeded onto 6 well plates coated with either 5–10 μg/mL collagen IV (10376931, Fisher Scientific) or 0.5 μg/mL iMatrix 511 (NP892-011 ...
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bioRxiv - Molecular Biology 2022Quote: ... The products were analyzed in a denaturing 6% polyacrylamide gel with a 5’-labeled RiboRuler Low Range RNA Ladder (Thermo Scientific) as size markers ...
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bioRxiv - Immunology 2022Quote: ... 100 μg of HDM or OVA were reconstituted in PBS with 0.1 M sodium bicarbonate at 1 mg/ml and mixed with 18 μg Texas Red-succinimidyl ester or 36 μg 5,(6)-TAMRA-succinimidyl ester (Life Technologies), respectively ...
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bioRxiv - Cancer Biology 2024Quote: ... Human embryonic stem cell line H9 was cultured on the 6-well plate coated with the Vitronectin (5 μg/ml) and in the Essential 8 Flex medium (both Thermo Fisher). Cells were split every 7 days using 5-minute incubation with 0.5 mM EDTA-PBS (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... and stained the same day of the experiment with the fluorescent dye 5(6)-TAMRA/SE™ (Thermo Fisher Scientific, C1171) at a final concentration of 1 μM (as in Wehrl et al ...
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bioRxiv - Genetics 2023Quote: ... Cultures were passaged every 5-6 days as small clumps by dissociation with a buffer containing 1 mg per ml Collagenase IV (ThermoFisher Scientific), 0.025% Trypsin (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... The injected individuals were kept at 18°C for 5 to 8 days in 6-well-plates (Nunc multidish no. 150239, Thermo Scientific) and then cultured at 22°C until sexual maturity ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.5 x 106 KELLY or 5 x 105 TET21N cells were transfected on a 6-well plate using 7.5 µl Lipofectamine RNAiMAX (Thermo Fisher Scientific) and 50 pmol siRNA according to the manufactureŕs instructions ...
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bioRxiv - Genomics 2022Quote: ... two-thirds of the cells were collected for single-cell sequencing and the remaining cells were re-plated on 6-well plates coated with 5 μg/cm2 Type I rat collagen (Gibco, A1048301). Two additional samples were collected on Days 11 and 21 for single-cell sequencing ...
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bioRxiv - Biophysics 2023Quote: ... 400 μg of calmodulin and myosin-5 expression vectors (at a 1:6 ratio) were mixed with 15 ml FreeStyle 293 media (Thermo Fisher) and 1.2 ml of PEI (Polysciences ...
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bioRxiv - Cancer Biology 2023Quote: ... 3×105 cells were seeded in a 6-well plate and transfected 24h later with 5 µg pX459 plasmid DNA using Lipofectamine 3000 (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were plated at a density of 1.5 × 10^5 per well on 6-well plates in Neurobasal media (Gibco 21103-049) containing B27 supplement (Gibco 17504-044) ...
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bioRxiv - Cell Biology 2024Quote: ... 2.5×10^5 cells per well were cultured overnight in a 6-well plate and surface labeled with NHS-LC biotin (Thermofisher#21335) at a concentration of 0.2 mg/ml in PBS for 20 minutes on ice ...
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bioRxiv - Microbiology 2024Quote: ... The presence of genomic SARS-CoV-2 RNA in these samples was evaluated by one-step RT-qPCR in a final volume of 5 μl per reaction in 384-well PCR plates using a thermocycler (QuantStudio 6 thermocycler; Applied Biosystems). The primers used targeted N-region viral gene ...
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bioRxiv - Immunology 2024Quote: ... B cells were isolated from spleens of C57BL/6 mice (MojoSort Mouse Pan B cells, 480052) and labeled with CellTrace violet (5 µM; Thermofisher, C34557) for 20 min at 37°C ...
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bioRxiv - Genetics 2021Quote: Live myofibers in the 6-well plate were stained with 2 µL of 5mg/mL of Hoechst (Molecular Probes, H1399) in 2 mL of 1X PBS for 5 minutes in a 37 °C with 5% CO2 incubator ...
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bioRxiv - Cell Biology 2021Quote: ... on 12 or 15 mm coverslips in a 6-well plate and 10 nM siRNA-1 or -2 was transfected using RNAi MAX reagents (Invitrogen) according the manufacturer protocol ...
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bioRxiv - Microbiology 2021Quote: ... for 6 min and seeding cells at a density of 2×106 cells/T75 flask (Nunc; Thermo Fisher Scientific, Singapore).
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bioRxiv - Microbiology 2021Quote: ... for 6 min and seeding cells at a density of 2×106 cells/T75 flask (Nunc; Thermo Fisher Scientific, Singapore).