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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for Erythromycin 90 95% Erythromycin A N N Dimethyl 13C2 ~90% 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... Membrane stain FM5-95 (final concentration of 5 μg/mL; Molecular Probes) and 0.5 mM IPTG were added to the solidified LB ...
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bioRxiv - Microbiology 2022Quote: ... extracted Chl a using DMSO (65 °C, 90 min) and then estimated Chl a content by spectrophotometry (665 and 750 nm; Genesys 20, Thermo Scientific, Waltham, MA), as in Caesar et al ...
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bioRxiv - Biophysics 2022Quote: Oocyte surface protein samples were denatured for 5 min at 90 °C in LDS sample buffer containing reducing agent (50 mM DTT) (NuPAGE®, Thermo Fisher Scientific). Protein samples (10-120 μg ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were run on 6% TBE gel in 1 x TBE (150 V, 90 min, 4°C) stained with SYBR Gold (Thermo Fisher Scientific. S11494) and InstantBlue Protein Stain (Expedeon).
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bioRxiv - Neuroscience 2021Quote: ... and slides were incubated for 90 min at room temperature with Goat secondary antibody to guinea pig IgG - Alexa Fluor 568 (1:200, Invitrogen, Waltham, MA, USA), goat secondary antibody to Rabbit IgG - H&L (Cy5 ...
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bioRxiv - Microbiology 2020Quote: ... the lysate was placed on ice for 5 min to cool down and 10 μL of the lysate was added to 90 μL RIPA buffer (Thermo Scientific, Waltham, MA) for determination of protein concentration ...
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bioRxiv - Microbiology 2020Quote: ... After 90 s this solution is removed and the cells are quickly washed with 37°C phosphate-buffered saline (PBS, Thermo Fisher, MA, USA). The second solution of 3 % GA in Citrate-buffered saline (CBS ...
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bioRxiv - Developmental Biology 2020Quote: ... the sections were washed twice in PBS and incubated at RT for 90 min with Alexa Fluor 488-conjugated goat anti-rabbit IgG (ThermoFisher Scientific, A-11008) and 594-conjugated goat anti-mouse IgG (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 90 min at room temperature with goat anti-mouse Alexa Fluor 594 (Cat. No. A11032, 1:250; ThermoFisher Scientific, Waltham, MA) and either of goat anti-rat Alexa Fluor 488 (Cat ...
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bioRxiv - Cancer Biology 2021Quote: ... to 125°C for 30 seconds and 90°C for 10 seconds in citrate (pH = 6.0, Thermo Scientific cat. no. Ap-9003-500) for Ascl1 and Zfp36l1 staining ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were cultured until 70-90% confluency at which point cells were dissociated from culture vessels using 0.05% Trypsin-EDTA (Thermo Fisher Scientific; Waltman, MA) and reseeded onto culture vessels for subsequent passaging ...
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bioRxiv - Cell Biology 2021Quote: ... the sections were washed twice in PBS and incubated at RT for 90 min with Alexa Fluor 488-conjugated goat anti-rabbit IgG (ThermoFisher Scientific, A-11008) secondary antibodies at a dilution of 1:2000 in PBS ...
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bioRxiv - Microbiology 2022Quote: ... two loopfuls of bacteria were taken from 3 different streaked colonies that grew without added inducer and inoculated directly in 90 μl phosphate-buffered saline (PBS) (Thermo Fisher Scientific 14200075). For genes expressed in E ...
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bioRxiv - Genomics 2022Quote: ... PBMCs were removed from liquid nitrogen storage and quickly thawed in 37◦C water bath before diluting with 6 ml of warm starvation media (90% RPMI 1640, 10% CS-FBS, 0.1% Gentamycin) and counting using Trypan blue staining on Countess II (Life Technologies Corporation, Bothell, WA). Cells were subsequently centrifuged at 400×g for 10 minutes and resuspended in culture medium at 2 × 106 cells/ml ...
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bioRxiv - Immunology 2022Quote: ... Cells with approximately 80–90% confluency were transfected with 1 μg of IVT ChulaCov19 using Lipofectamine™ MessengerMax™ (Invitrogen, Carlsbad, CA, USA) according to the manufacture’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... subunits was added to cell layers that were 70-90% confluent using Lipofectamine 2000 transfection reagent (catalog #11668-027, ThermoFisher Scientific, Waltham, MA) following manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... They were grown at 37 ◦C in an atmosphere of 5% CO2 until 90 % confluence in complete DMEM culture medium (from GIBCO BRL; Life Technologies, Paisley, Scotland) supplemented with 1% glutamine ...
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bioRxiv - Plant Biology 2023Quote: ... Tissue was grounded in liquid nitrogen and combined with 500 μl of pre-chilled 90% methanol (A452-1, Fisher Scientific, Hampton, NH, USA) vortexed and finally incubated for 4h on ice ...
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bioRxiv - Pathology 2023Quote: Slides were incubated at 90°C for 20 minutes in High pH IHC Antigen Retrieval Solution (Thermo Fisher Scientific Catalog Number 00-4956) and allowed to cool to room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... The iPSCs were passaged every 4 - 5 days when it reached 80 - 90% confluency using the StemPro® EZPassage™ disposable stem cell passaging tool (23181-010, Life Technologies). The cells were passaged as a small colony with a split ratio of 1:6 and cultured by changing the medium every day ...
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bioRxiv - Evolutionary Biology 2023Quote: ... muscarius was isolated from Mycotal (Koppert, NL) and spread on Sabouraud dextrose agar (SDA) in 90 mm triple vented Petri dishes (Fisher Scientific, Loughborough, UK). Isolation and production of conidia involved incubation in darkness at 23±1 °C on SDA plates for 10-14 days ...
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bioRxiv - Cell Biology 2023Quote: ... Pericytes were passaged at 60%-90% confluence by washing with Dulbecco’s phosphate buffered saline without magnesium or calcium (DPBS-/-, ThermoFisher Scientific, cat.#14190-144) prior to treatment with TrypLE Express (Thermofisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... All cells were counted by the following method: 10 µL of cell suspension from samples was added to 90 µL 0.04% Trypan blue in PBS (Gibco™; Ref. 15250-061) for a 1:10 dilution ...
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bioRxiv - Bioengineering 2024Quote: ... The iPSCs were passaged every 4 - 5 days when it reached 80 - 90% confluency using the StemPro® EZPassage™ disposable stem cell passaging tool (23181-010, Life Technologies). The cells were passaged as small clumps with a 1:6 split ratio and cultured with daily medium change ...
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bioRxiv - Developmental Biology 2024Quote: ... POI-Halo organoids were seeded in 10-20 μL 90% Matrigel droplets into 8-well Labteks II #1.5 (Nunc, cat.# 12-565-338) and overlaid with 500 μL IntestiCult ...
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bioRxiv - Cell Biology 2019Quote: ... Chambers were then flushed with 25 ug/ml neutravidin (Invitrogen, #A-2666) in assay buffer containing 200 ug/ml kappa-casein and instantly rinsed with assay buffer followed by warm up to RT ...
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bioRxiv - Systems Biology 2021Quote: ... both cell types were stained with Hoechst (10 ug/ml, Life Technologies) to pick cells in G0/G1 phase ...
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bioRxiv - Cancer Biology 2020Quote: ... Transfected cells were selected with zeocin (300 ug/ml; Gibco/Life Technologies) and sorted for double positive cells (anti-HA-AF488 and anti-EGFR-AF647 ...
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bioRxiv - Cancer Biology 2020Quote: ... Transfected cells were selected with zeocin (300 ug/ml; Gibco/Life Technologies) and sorted for double positive cells (anti-HA-AF488 and anti-EGFR-AF647 ...
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bioRxiv - Cancer Biology 2022Quote: ... heparin salt (4 ug/ml) and pen/strep (1% v/v) (Invitrogen) was used to culture the spheroids ...
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bioRxiv - Biophysics 2022Quote: ... and HCS CellMask™ Deep Red Stain (2.5 ug/ml, ThermoFisher, #H32721). Staining with the Golgi dye from the Golgi Staining Kit was performed following the instructions of the provider ...
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bioRxiv - Cell Biology 2021Quote: ... Hoechst 33528 (1-10 ug/mL) or Syto DeepRed (1:1000, ThermoFisher) were used for counterstaining DNA.
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bioRxiv - Genomics 2023Quote: ... 1 ug/mL Laminin mouse protein (Thermo Fisher Scientific; Cat. No. 23017015), 10 nM ROCK inhibitor ...
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bioRxiv - Genomics 2023Quote: ... 1 ug/mL concentration of puromycin (GIBCO/Thermo Fisher Scientific, Cat. No.A1113803) was added to cells to select for successfully transfected cells over a period of 4 days.
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bioRxiv - Genetics 2023Quote: ... and selected infected cells with 2 ug/ml puromycin (Thermo Fisher Scientific). Cells were maintained in 0.5 ug/ml puromycin for the duration of the experiment to maintain gRNA expression ...
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bioRxiv - Systems Biology 2023Quote: ... the cells were selected using 1 ug/ml of puromycin dihydrochloride (ThermoFisher), then grew until 80-90 % confluency ...
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bioRxiv - Cell Biology 2024Quote: ... iSLK-BAC16 derivatives were also maintained in 10 ug/mL blasticidin (Gibco). For lytic infection ...
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bioRxiv - Biophysics 2024Quote: ... 20 ug/ml BSA) using Zeba 5K desalting spin columns (ThermoFisher Scientific). The variants were subjected to oxidation by mixing σ70 variant at 2.5 μM final concentration with hydrogen peroxide at 5 mM final concentration in buffer R ...
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bioRxiv - Genetics 2021Quote: ... using a Leica M205C stereomicroscope (190-240X magnification) and collected directly into sterile 0.2 mL PCR flat-cap tubes containing Prep-n-GoTM Lysis buffer (ThermoFisher Scientific, Carlsbad, CA, USA). This was done via a sterile tungsten needle and water-soluble 3MTM adhesive ...
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bioRxiv - Plant Biology 2021Quote: ... A 0.5 g sample of the collected tissues were ground under liquid nitrogen and suspended in 1 ml of co-IP extraction buffer (1× PBS, 1% n-dodecyl-β-d-maltoside (Invitrogen, Carlsbad, CA), protease inhibitor cocktail cOmplete™ (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 ng/mL (n = 3) and 10 ng/mL (n = 3) treatment were subjected to albumin depletion according to the manufacturer’s instructions (Thermo Fisher Scientific, Loughborough, UK). Individual samples were digested with trypsin (2.5µg trypsin ...
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bioRxiv - Microbiology 2021Quote: ... PM-WCPV-S) or N peptide pool (Meltenyi, 130-126-698) in the presence of 1 µg/mL anti-CD28 (Invitrogen, 14-0281-86) for co-stimulation for 6 hours ...
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bioRxiv - Biophysics 2019Quote: ... Cleaved peptides were precipitated from ice-cold methyl tert-butyl ether (MTBE; Thermofisher Acros Organics, Geel, Belgium). After washing and collection by centrifugation crude peptides were dissolved in 20% (v/v ...
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bioRxiv - Biophysics 2019Quote: ... Cleaved peptides were precipitated from ice-cold methyl tert-butyl ether (MTBE; Thermofisher Acros Organics, Geel, Belgium). After washing and collection by centrifugation crude peptides were dissolved in 20% (v/v ...
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bioRxiv - Bioengineering 2021Quote: ... in dimethyl sulfoxide (Thermo Fisher) at a 40-molar excess of D-biotin to streptavidin and incubation for 30 minutes while shaking at 600 RPM.
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bioRxiv - Genetics 2021Quote: ... Primary antibodies used were: 1:1000 mouse anti-SARS/SARS-CoV-2 N (ThermoFisher Scientific; ...
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bioRxiv - Physiology 2022Quote: ... 1,5 mM sodium pyruvate (catalog n°11360-070, Gibco, Thermofisher Scientific, Waltham, MA, USA) and pCPT-cAMPC (catalog n°C3912 ...
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bioRxiv - Molecular Biology 2020Quote: ... HCT116 cells were transfected with pCDNA-N-FLAG-SUPT5H-variants using Lipofectamine 3000 (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... were cultured in GlutaMAX containing Dulbecco’s Modified Eagle Medium (DMEM) (Invitrogen, cat n°31966047). The HAP1 wild type and CRISPR/Cas9 edited human knockout cell lines obtained from Horizon Genomics GmbH (Vienna ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA/transfection reagent complexes in 500 μl Opti-MEM (Invitrogen, cat n°51985-042) were allowed to form by mixing and incubation at room temperature for 10 min ...