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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for Erythromycin 90 95% Erythromycin A N N Dimethyl 13C2 ~90% 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... The hiPSC were passaged at 70-90% confluency by washing with DPBS and then adding Accutase cell dissociation reagent (ThermoFisher A1110501) and incubating at 37 °C for 4 mins ...
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bioRxiv - Developmental Biology 2022Quote: ... The hiPSC were passaged at 70-90% confluency by washing with DPBS and then adding Accutase cell dissociation reagent (ThermoFisher A1110501) and incubating at 37 °C for 4 mins ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were grown in 150 mm dishes to ∼90% confluence then washed with sterile PBS and detached (Gibco TrypLE Express #LTS12604021). Cells used for all experiments were ≤9 passages from stock and tested negative for mycoplasma contamination (Agilent MycoSensor QPCR Assay Kit ...
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bioRxiv - Cancer Biology 2021Quote: ... the tryptic peptides were separated by increasing concentrations of 80% ACN/0.1% formic acid at a flow of 250 nL/min for 90 min and analyzed with a QExactive HF mass spectrometer (Thermo Scientific). The instrument was operated in the data dependent acquisition mode to automatically switch between full scan MS and MS/MS acquisition ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA of N-terminal Flag-tagged wild-type human eIF4A1 was amplified by PCR and subcloned into the pLenti-CMV-Puro-DEST (w118-1) vector 90 using the Gateway cloning system (Thermo Fisher). The TIMMDC1 ...
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bioRxiv - Developmental Biology 2022Quote: ... hESCs are obtained from WiCell (RRID:CVCL_9772) maintained and split from around 90% confluence with 1x versene (Fisher Scientific 15-040-066) for 5 minutes at RT and then split with 1:8 ratio into 6 well plates pre-coated with 1:200 diluted geltrex in DMEM/F12 medium (Thermo Scientific A1413201 ...
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bioRxiv - Developmental Biology 2022Quote: ... The hiPSC were passaged at 70-90% confluency by washing with DPBS and then adding Accutase cell dissociation reagent (ThermoFisher A1110501) and incubating at 37 °C for 4 mins ...
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bioRxiv - Microbiology 2019Quote: Cells were cultured in 6-well plate to 90% confluency and total RNA was extracted by Trizol (Life Technologies, Carlsbad, CA) and RT-qPCR was performed following the instruction of the manufacturer ...
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bioRxiv - Bioengineering 2019Quote: ... HEK-293T cells were grown to 70-90% confluency in Dulbecco’s modified Eagle medium containing 10% fetal bovine serum (Thermo Fisher Scientific), washed with PBS ...
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bioRxiv - Bioengineering 2019Quote: ... Luria Bertani (LB) broth (#BP1426-500) and tissue culture polystyrene 96-well plates (#07-200-90) were purchased from Fisher Scientific, and bioluminescent ilux pGEX(- ...
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bioRxiv - Immunology 2021Quote: ... MoDCs (50,000/well) were loaded for 60–90 min at 37◦C with peptide and labelled with Cell Trace Violet (Thermo Fisher) to distinguish them from T cells prior to co-culturing with 50,000 T cells/well in a 96 well plate for 24 h ...
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bioRxiv - Immunology 2020Quote: ... MoDCs (50,000/well) were loaded for 60–90 min at 37°C with peptide and labelled with Cell Trace Violet (Thermo Fisher) to distinguish them from T cells prior to co-culturing with 50,000 T cells/well in a 96 well plate for 24 h ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell differentiation was induced when cells reached around 90% confluence by replacing the proliferation medium with 99% (v/v) high glucose DMEM (Gibco, UK), 1% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... The fruit were dried off and two fruit were placed into each sterile 90 mm triple-vented petri dish bottom or lid (Thermo Scientific). The petri dish lids and bottoms with fruit were placed on trays sterilised with 70% ethanol ...
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bioRxiv - Biochemistry 2019Quote: At 90% confluency the cells were aspirated in serum free RPMI 1640 GlutaMAX medium with 1% Penicilin/streptomycin (Invitrogen # 15070-063), 1mM sodium pyruvate (sigma Aldrich #S8636 ...
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bioRxiv - Genomics 2021Quote: ... cells were cultured on gelatin-coated 6-wells in 60% BRL conditioned medium to 70-90% confluency and transfected with Lipofectamin3000 (Invitrogen L3000008) according to the supplier protocol with 2 μg donor vector and 1 μg Cas9/guide vector ...
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bioRxiv - Neuroscience 2020Quote: ... Peripheral blood monocytes (PBMCs) were collected and resuspended in freezing media (90% v/v fetal bovine serum (FBS, Thermofisher, CA, USA) supplemented with 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HK-2 was seeded in 6 well plates (1 × 106 cells/well) or 96 well plates (1 × 105 cells/well) and cultured in 90% RPMI1640 medium (Gibco, USA) supplemented with 100 U/mL penicillin ...
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bioRxiv - Biochemistry 2021Quote: Cells were transfected at 90% confluency in 60 mm dishes and using 5 µg DNA and lipofectamine 2000 (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Size exclusion fractions enriched in crosslinked peptides were combined into two mass spectrometry samples and each one was acquired over a 90 minute UPLC gradient acquiring sequential HCD and EThcD product ion spectra on a Fusion Lumos mass spectrometer (Thermo Scientific). Peaklists were generated using PAVA for each dissociation method and searched with Protein Prospector 6.3.23 ...
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bioRxiv - Developmental Biology 2021Quote: ... Peptides were eluted along a 90 min gradient from 6 to 40% Buffer B (see StageTip purification) with the EASY□nLC 1,200 system (Thermo Fisher Scientific). Measurement was done on an Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... Reporter plasmids (2 μg) were transfected into HEK-293 cells in 6-well plates at ∼60-90% confluency using Lipofectamine 3000 (ThermoFisher Scientific). Cells were harvested after 1 day by aspirating the media and resuspending the cells in 1 mL Trizol reagent (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... was denatured for 10 minutes at 90°C and loaded onto a Bolt BT Plus 4-12% gel (Invitrogen, Carlsbad, CA). After electrophoresis ...
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bioRxiv - Biochemistry 2022Quote: ... Between 2 to 3 million cells were used per electroporation and were resuspended in 90 μl of buffer R (Thermo Fisher). Proteins were spun down at 16700 g for 10 minutes and then diluted with buffer R in a 1:1 ratio ...
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bioRxiv - Bioengineering 2022Quote: Human fetal lung fibroblast (Institute for Medical Research-90, IMR90, ATCC) cells were cultured in Minimum essential medium (MEM, 1×) (Invitrogen, USA) containing 10% fetal bovine serum (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... at 300 nL/min over 90 minutes and eluted through a nanospray interface into a Q-Exactive Orbitrap mass spectrometer (Thermofisher Scientific). Qualitative and label-free quantification was performed using Proteome Discoverer 2.3 37 ...
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bioRxiv - Immunology 2022Quote: ... The media was then removed from each well and replaced with 90 μL fresh media and 10 μL PrestoBlue reagent (ThermoFisher Scientific) and incubated at 37°C and 5% CO2 for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... Phage DNA integrity was checked using agarose gel electrophoresis (1 % agarose gel in 1× TAE buffer; 70 V, 90 min) against a GeneRuler 1 kb ladder (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... blocked again for 2h (10% CAS-Block / 90% PBT) and incubated overnight at 4°C with secondary antibody (1:250, Alexa-Fluor-594, ThermoFisher A32742) diluted in 100% CAS-Block ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were then incubated for 90 min at room temperature with secondary antibodies (1:500, Alexa Fluor 568, Alexa Fluor 488, Life Technologies). After three rinses ...
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bioRxiv - Physiology 2022Quote: Production of NO was measured in HUVEC exposed to BPS for 30 or 90 min by loading with a DAF-FM Diacetate probe (Invitrogen, D23844) for 45 min at 37°C and quantifying on a SpectraMax M2 plate reader or flow cytometer ...
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bioRxiv - Molecular Biology 2022Quote: IP samples were eluted with 40 µl 1xLDS buffer adjusted to 10 mM DTT (5 min, 90°C) and separated on 4-12% Bis-Tris NuPAGE gels (Thermo Fisher) followed by colloidal coomassie staining (Instant Blue ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant containing the cells was filtered through 70-μm strainers into a tube containing 90% isotonic Percoll (GE Healthcare, #17-0891-01, in 10□×□PBS pH 7.2 (Gibco, #70013032). The final volume was topped up with DMEM/F12 (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were multiplexed and analyzed by LC-MS/MS using a 90-minute gradient on Orbitrap Eclipse (Thermo Fisher, Waltham, USA) with a method using CID-MSA for the identification of MS2 and a real-time search algorithm to quantify reporters in MS3 ...
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bioRxiv - Microbiology 2023Quote: ... Growth medium on HEK293T cells at 90% confluency in 6 well plates was replaced with Opti-MEM reduced serum medium (Thermo Fisher) and cells in each well were transfected with 250ng each of 8 pDUAL reverse genetics plasmids ...
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bioRxiv - Cancer Biology 2023Quote: ... standard curve analysis was carried out to ensure that the amplification efficiency of PCR for each primer pair was within the prescribed range of 90-110% (Applied Biosystems). Quantitative PCR (qPCR ...
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bioRxiv - Immunology 2023Quote: NPK-C1 cells at 70-90% confluence or MC38 cells at 50-75% confluence were harvested with 0.05% trypsin (Gibco, Gaithersburg, MD), washed with PBS ...
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bioRxiv - Genetics 2023Quote: ... primary myoblasts were labelled for 90 minutes and processed with EdU Click-iT™ EdU Cell Proliferation Kit (C10337, ThermoFisher, GmbH). Percentage of EdU+ nuclei was determined after normalizing the EdU+ nuclei to DAPI stained nuclei.
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bioRxiv - Microbiology 2023Quote: ... Cells at 80∼90% confluence were transfected with 200 ng of pCAGGS derivates using Lipofectamine® 2000 (11668027, Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... or 90 pmol of SBP-tagged eIF4A1 protein was incubated with 30 μl of Dynabeads M-270 Streptavidin (Thermo Fisher Scientific), which had been preequilibrated with equilibration buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... cell concentration and viability (80-90%, see fig. S1B) of each cell suspension were determined using 0.4% trypan blue (Gibco™: 15250061). Cells were washed and resuspended in 1x PBS (calcium and magnesium free ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then blocked with a 5% (v/v) FBS solution and incubated for 90 min in a 1:200 phalloidin–Alexa Fluor 594 (Life Technologies) diluted in a 1% (v/v ...
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bioRxiv - Genomics 2024Quote: ... to a final volume of 200 μL and then incubated 90 min at 37°C with 6 μL of RNase cocktail (Ambion, AM2286), followed by 150 minutes at 55°C with Proteinase K (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... The viral media overlayed on the sorbitol cushion was applied to a Sorvall WX-90 ultracentrifuge and SureSpin 630 rotor (Thermo Scientific) at 20,000 rpm for 1.5 hours at 18°C ...
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bioRxiv - Systems Biology 2024Quote: ... using a 98 min gradient with a 250 nl/min flow rate of increasing Buffer B (90% ACN, 0.1% FA) concentration (from 2% to 60%) on a High Performance Liquid Chromatography (HPLC) system (Thermo Fisher Scientific) and analyzed on a Q Exactive Plus instrument (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... at concentrations ranging from 1.6-16 mM for 90 minutes before being detached from the surface by treatment with 0.25 % trypsin-EDTA (Thermo Fisher Scientific). The detached cells were then centrifuged at 350 x g for 5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... were formed by incubating 90 pmol sgRNA and 10 pmol Cas9-2NLS in Buffer R (Component of Neon Transfection System Kit; Thermo Scientific) at room temperature for 10 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... reaction were applied to grids for 60 seconds at room temperature and 90% humidity in a Vitrobot Mark IV (Thermo Fisher). Grids were double-side plotted with force 0 for 5 seconds and immediately plunge frozen in liquid ethane ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were eluted from the column using a linear acetonitrile gradient from 2 to 30% acetonitrile over 90 minutes followed by high and low organic washes for another 20 minutes into an LTQ XL mass spectrometer (Thermo Scientific) via a nanospray source with the spray voltage set to 1.8kV and the ion transfer capillary set at 180°C ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... BJ-hTERT and IMR-90 cells were cultured in DMEM supplemented with 10% FBS and 2 mM L-glutamine (Gibco, 25030081). All media were supplemented with penicillin (100 U/ml)–streptomycin (100 μg/ml ...