Labshake search
Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for Erythromycin 90 95% Erythromycin A N N Dimethyl 13C2 ~90% 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... in six-well feeder-free culture dishes coated with 5 μg/mL vitronectin (VTN-N; Thermo Fisher Scientific). When seeding the cells ...
-
bioRxiv - Cell Biology 2021Quote: ... Western blots were carried out using iBind with 1 ug/ml primary antibodies and 1 ug/ml secondary antibodies following iBind protocol (Invitrogen/ThermoFisher).
-
bioRxiv - Physiology 2020Quote: ... and incubated with 100 mM 6-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (6-NBDG) (Life Technologies) in 10 nM Tris/HEPES buffer containing 150 mM KCl or 150 mM NaCl for 30 minutes at 37 °C ...
-
bioRxiv - Molecular Biology 2023Quote: ... and once with 1X PBS for 5 min each before blocking with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, ThermoFisher) in NHS-acetate buffer (0.1M NaP ...
-
bioRxiv - Bioengineering 2023Quote: ... They were cultured on 100-mm Petri dishes coated with human VTN-N truncated recombinant protein (Gibco, Thermo Fisher Scientific). The hiPSCs obtained from Thermo Fisher Scientific were maintained in Essential 8 Medium (Gibco ...
-
bioRxiv - Bioengineering 2023Quote: ... They were cultured on 100-mm Petri dishes coated with human VTN-N truncated recombinant protein (Gibco, Thermo Fisher Scientific). The hiPSCs obtained from Thermo Fisher Scientific were maintained in Essential 8 Medium (Gibco ...
-
bioRxiv - Cancer Biology 2023Quote: ... 0.1M Tris-base in DEPC H2O) and 1X PBS for 5 min each before being blocked using 100 mM N-succinimidyl acetate (NHS-acetate, ThermoFisher) in NHS-acetate buffer (0.1M NaP ...
-
bioRxiv - Molecular Biology 2021Quote: ... 95 mL of filtered ES FBS (Gibco 16141-061), 5.9 mL of antibiotics (Hyclone SV30079.01) ...
-
bioRxiv - Biochemistry 2022Quote: ... 10 mM HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) and 1% (v/v) penicillin/streptomycin (P/S) (all from Thermo Fisher Scientific, Waltham, MA, USA). Then ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... MTBE (≥99.5%) and methanol (≥99.8%) for HPLC analysis were obtained from Fisher Scientific GmbH and acetone for extraction and standard dilutions from Carl Roth GmbH (≥ 99.9%) ...
-
bioRxiv - Cell Biology 2020Quote: Near-confluent (80-90%) MEFs were transfected with 150 nM lamellipodin or Rac1 siRNAs using Lipofectamine 2000 reagent (Invitrogen) in reduced serum Opti-MEM as previously described [1 ...
-
bioRxiv - Microbiology 2021Quote: ... Radiolabeled RNA was incubated 5 min at 90°C with alkaline buffer or 5 min at 37°C with ribonuclease T1 (0.1 U; Ambion) to generate the Alkaline (OH ...
-
bioRxiv - Neuroscience 2020Quote: ... The medium was changed every day and cells were passaged at 80%–90% confluency using TrypLE Express Enzyme (Gibco) supplemented with 10 μM Y-27632 (Stem Cell Technologies ...
-
bioRxiv - Microbiology 2020Quote: Stock solutions were diluted in DMSO and 2µL of the resulting solutions plus 148µL of a bacterial suspension standardized to an OD600 of ∼ 0.1 and diluted 1:500 in 10:90 was added to a 96 well plate (Nunc) in triplicate ...
-
bioRxiv - Neuroscience 2020Quote: ... third instar larvae were dissected and stimulated with a HL-3 solution of 90 mM K+/0.5 mM Ca2+/4 μM fixable FM1-43 (Invitrogen) for 1 min ...
-
bioRxiv - Synthetic Biology 2020Quote: ... the cells were diluted 1:10 (10 μL into 90 μL) in phosphate-buffered saline (PBS) (Gibco,18912-014) containing 2 mg/mL kanamycin to halt protein translation and incubated at room temperature for 1 hour to allow for maturation of the YFP before performing flow cytometry.
-
bioRxiv - Cell Biology 2019Quote: ... boiled for 5 min at 90°C and loaded on NuPAGE Novex 4–12% Bis-Tris Protein Gels (Invitrogen). Gels were then fixed for 30 min at RT in fixing solution (40% [v/v] methanol ...
-
bioRxiv - Cancer Biology 2019Quote: The immortalised non-tumorigenic human epithelial cell line MCF12A (ATCC, CRL-10782),90 were cultured in DMEM/F12 (Gibco) supplemented with 5% horse serum (Sigma) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Samples were reconstituted in 90 µl chloroform and incubated with 10 µl MethPrepII (Thermo Fisher Scientific, Waltham, MA, USA) for 20 min at room temperature.
-
bioRxiv - Microbiology 2021Quote: ... supplemented with 10% fetal bovine serum (Genclone) and passaged at 80-90% confluence with 0.05% trypsin-EDTA (25300-062, Gibco). All cell lines were routinely tested for the presence of mycoplasma ...
-
bioRxiv - Molecular Biology 2021Quote: ... Upon 70-90% confluency iPSCs were washed 3 times with PBS and subsequently passaged in colonies using 0.5 mM EDTA (ThermoFisher) in PBS for 5 minutes at room temperature ...
-
bioRxiv - Developmental Biology 2022Quote: ... After multiple 5-min washes in PBS and 90-min incubation with AlexaFluor-conjugated secondary Ab as above (Invitrogen) at room temperature ...
-
bioRxiv - Neuroscience 2021Quote: ... The samples were transferred to nitrocellulose with a semi-dry transfer system (Life Technologies Inc.: 65V/gel, 90 min). Membranes were blocked for 30 min with 5% milk in PBS containing 0.1% Tween 20 at 25 °C ...
-
Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... The membrane was UV crosslinked (120 mjoules/cm2) using a Stratalinker Spectroline™ XL-1500 (ThermoFisher,11-992-90). Probe was labeled with T4 PNK (NEB M0201S ...
-
bioRxiv - Microbiology 2021Quote: ... cell lysates and virions pelleted through 20% sucrose (14,000xg for 90 minutes at 4°C) were separated on a NuPage 4-12% Bis-Tris Gel (Invitrogen) and subsequently blotted onto a nitrocellulose membrane ...
-
bioRxiv - Microbiology 2022Quote: ... and TMPRSS2/pLX304 or ACE2/pLJM1 (1.64 pmol; gifts from Sean Whelan) 90 µL lipofectamine 3000 (Thermo Scientific L3000001), 1ul psPAX2 (1.3 pmol ...
-
bioRxiv - Microbiology 2022Quote: ... at 90°C for 10 min before being run on a 4-12% acrylamide denaturing gel (ThermoFisher, #NP0323 NuPAGE). Proteins were then transferred onto a nitrocellulose membrane (ThermoFisher ...
-
bioRxiv - Immunology 2022Quote: ... Samples were denatured at 90°C for 10min in the presence of 4x LDS Sample Buffer (NP0007, Invitrogen, USA) and 10x Sample Reducing Agent (B0009 ...
-
bioRxiv - Cell Biology 2019Quote: ... At 90% confluence the cells were transfected with 30 nmol/L siRNA using Lipofectamine RNAi MAX (# 13778, Invitrogen, MA) in OptiMEM according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2019Quote: ... supernatant was removed and cells re-suspended in 90 µl of Schneider’s medium containing 20% fetal calf serum (Gibco). 30 μl drops were placed on cover slips ...
-
bioRxiv - Cell Biology 2019Quote: ... and incubated for 5min at 90°C before loading on pre-cast 4-12% Bis-Tris acrylamide gel (Invitrogen). Subsequently ...
-
bioRxiv - Plant Biology 2020Quote: ... Samples were extracted in 90% aqueous methanol (Sigma-Aldrich HPLC Plus grade methanol and Fisher Scientific Optima™ water) using 10 μL solvent per mg of fresh-frozen tissue ...
-
bioRxiv - Physiology 2021Quote: ... culture medium of cells at 90% confluence was replaced with DMEM containing 2% horse serum (26050070, Gibco, NY, USA). Cells were cultured in the differentiation medium for another 5 days until formation of myotubes.
-
bioRxiv - Molecular Biology 2019Quote: Total RNA was isolated from 10 cm2 of cells grown standardly to 80-90% confluence with TRIzol reagent (Invitrogen), following the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2021Quote: ... Single-cell suspensions <40μm were cryogenically stored in 5μM StemMACS™ Y27632 90% heat-inactivated fetal bovine serum (Gibco)/10% dimethyl sulfoxide (Invitrogen) ...
-
bioRxiv - Biochemistry 2021Quote: ... Samples were run at 4 °C for 90 min and then stained 30 min with SYBR™ Gold (ThermoFisher). Gels were imaged on a fluorescence Typhoon imager with the excitation and emission set to 480 nm and 520 nm ...
-
bioRxiv - Cancer Biology 2019Quote: ... Media was changed every 3 to 4 days and cells were passaged at nearly 90% confluence using 0.25% trypsin (Gibco). B16F0 cells were verified for mycoplasma contamination at Charles River Laboratories for cell line testing ...
-
bioRxiv - Cancer Biology 2020Quote: ... Any remaining tumor tissue was deposited into freezing media consisting of 90% heat-inactivated fetal bovine serum (FBS) (Invitrogen) and 10% dimethyl sulfoxide (Sigma-Aldrich) ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... and transferred to into 150 µl ice-cold Schneider’s Drosophila medium and fetal bovine serum (SDM+FBS, 90:10 V/V; both ThermoFisher). After centrifugation at 250x g for 2 min ...
-
bioRxiv - Immunology 2020Quote: ... or cell preservation tubes (CPT; Becton Dickinson) according to the manufacturer’s instructions and cryopreserved using 90% fetal bovine serum (Gibco) 10% DMSO (Sigma Aldrich ...
-
A stable reference human transcriptome and proteome as a standard for reproducible omics experimentsbioRxiv - Cell Biology 2022Quote: Cells of each generation were cultured to 80–90% confluency and treated with 0.25% trypsin-EDTA (Gibco, 25200056, USA), centrifuged at room temperature at 300 xg for 5 min ...
-
bioRxiv - Cell Biology 2022Quote: ... purchased from IDT) into MEFs and IMR-90 at 50-70% density was achieved using Lipofectamine RNAiMax (Invitrogen #1847641) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: HT1080 were cells grown to 90% confluency and transfected with the DsiRNAs using Lipofectamine RNAiMAX (ThermoFisher Scientific, Waltham, Massachusetts) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... mRNA encapsulation was about 90% (measured by RiboGreen assay – Quant-iT™ RiboGreen™ RNA Reagent, Thermo Fisher Scientific).
-
bioRxiv - Microbiology 2022Quote: ... A spleen was removed from the animal and collected in media (90% RPMI 1640 (Gibco Cat. 11875093, Waltham, MA) + 10% FBS (VWR Cat ...
-
bioRxiv - Immunology 2022Quote: ... PBMCs were suspended in freezing media (90% (v/v) foetal bovine serum (FBS; Gibco/Thermo Fischer Scientific, Waltham, MA) and 10% (v/v ...
-
bioRxiv - Immunology 2022Quote: HEK 293T cells were maintained between 10% and 90% confluency at 37°C/5% CO2 in DMEM (Life Technologies) supplemented with 10% foetal bovine serum (Sigma) ...
-
bioRxiv - Cell Biology 2022Quote: ... Cell lysates were incubated overnight with 90 μL of Dynabeads® MyOne™ Streptavidin C1 (Invitrogen, Carlsbad, CA, USA) on a shaker at 4ºC ...
-
bioRxiv - Biophysics 2024Quote: Transfection was performed on 70%-90% confluent tsA-201 cells using the lipofectamine 3000 Kit (Invitrogen, Carlsbad, CA, #L30000080), following previously established protocols47,74 ...
-
bioRxiv - Physiology 2023Quote: Transfection was performed on 70%-90% confluent tsA-201 cells using the lipofectamine 3000 Kit (Invitrogen, Carlsbad, CA, #L30000080), following previously established protocols43,70 ...