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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for 6 Benzyloxy 1H indole 2 boronic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% formic acid in acetonitrile) on an EASY-Spray column ES801 (50 µm ID × 15 cm, 2 µm; Thermo Scientific) mounted on a DPV ion source (New Objective ...
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bioRxiv - Biochemistry 2024Quote: ... was labeled with the fluorescent probe 5-((((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (1,5-IAEDANS from Molecular Probes) as previously described (17 ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... Cells were trypsinised and resuspended in FACS buffer (1ξ Ca2+ and Mg2+ free PBS, 2 mM ethylenediaminetetraacetic acid (Life Technologies), 2% FBS Bovogen) ...
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bioRxiv - Cancer Biology 2024Quote: Peptides were reconstituted with 2% formic acid and analyzed in triplicates on an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific) coupled to an UltiMate 3000 UHPLC system (Thermo Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... were grown in high-glucose DMEM with supplements (1% penicillin/streptomycin, 10% FBS, 1% Non-essential Amino Acids [NEAA; Wako], 55 µM 2-Mercaptoethanol [Gibco]). Mouse embryonic stem (ES ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were resuspended in 2% acetonitrile in 0.1% formic acid and loaded at 5 μL/minute onto a PepMap C18 trap column (Thermo Scientific; 5 μm 100 Å particle size ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were then dissolved in 2% ACN and 0.1% formic acid and analyzed on a Thermo Scientific Q Exactive Mass Spectrometer (ThermoFisher Scientific). The MS was operated in data-dependent mode ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA and RNA products at various points of the iTP-seq protocol were analyzed on 9% acrylamide (19:1) TBE (90 mM Tris, 90 mM boric acid, and 2 mM EDTA) gels and stained with SyBR Gold (Invitrogen). Inverse toeprints were excised from 12% acrylamide TBE gels using a clean scalpel ...
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bioRxiv - Physiology 2024Quote: ... fractions were resuspended in 2% acetonitrile and 0.1% formic acid and separated on an Easy nLC 1200 (Thermo Fisher Scientific) and a 35 cm long ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 µM × 250 mm) in Solvent A (98% water, 2%acetonitrile, 0.1% formic acid) via a Nanospray Flex Ion-Source (Thermo Scientific) at a voltage of 1.9 kV with a flow rate of 0.7 µL/min in Solvent A (98% water/2%acetonitrile/0.1% formic acid) ...
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bioRxiv - Microbiology 2024Quote: ... For buffered growth mDMEM-DMP was supplemented with 100 mM of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Gibco 15630056).
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bioRxiv - Physiology 2024Quote: ... hearts were loaded with 1 mg/ml Rhod-2 AM in 0.5 mL of DMSO containing 10% pluronic acid (Molecular Probes) followed by 50 µL 1 mg/ml RH237 in DMSO (Molecular Probes) ...
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bioRxiv - Microbiology 2024Quote: ... 750 mM aminocaproic acid, 2 mg/ml lysozyme, 1 mM EDTA, 1.43 mM BME, 1x EDTA-free Pierce Protease Inhibitor [Thermo Scientific]) and incubated 20 min on ice ...
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bioRxiv - Molecular Biology 2024Quote: ... from day 1-2 Sprague-Dawley rats and maintained in DMEM (L-glutamine, Sodium Pyruvate, Non-essential amino acids; Gibco) supplemented with 2% FBS (Gibco ...
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bioRxiv - Biophysics 2024Quote: We monitored the change in absorbance over time of 5,5’-dithiobis-(2-nitrobenzoic acid) (DTNB, Ellman’s reagent, Thermo Fisher Scientific). Various concentrations (100 -1000 μM ...
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bioRxiv - Bioengineering 2024Quote: ... with the pellet re-suspended in 0.1 M (2-(N-morpholino)ethanesulfonic acid) (MES) buffer at pH 5.5 (Fisher Scientific 15442958). This process was repeated four times ...
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bioRxiv - Cancer Biology 2024Quote: ... WiT49 + sgNTC-2 cells were transfected with 10 µg/ml polyinosinic-polycytidylic acid (Poly I:C) with Lipofectamine 3000 (Invitrogen L3000015). The next day ...
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bioRxiv - Cell Biology 2024Quote: ... or BODIPY 558/568 C12 (C12) (4,4-Difluoro-5-(2-Thienyl)-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (ThermoFisher, #D3835) were complexed with fatty acid-free bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 1h with NeuroTrace 530/615 Red Fluorescent Nissl Stain (1:500, ThermoFisher Scientific) on a shaker at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then harvested and stained for 1h at room temperature with 8 μM CCF4-AM (Invitrogen) in EM medium supplemented with 2.5 μM probenecid ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then incubated for 1h with anti-rabbit Alexa Fluor 568 secondary antibodies (Molecular Probes-Invitrogen) diluted at 1:500 ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then incubated for 1h with anti-rabbit Alexa Fluor 568 secondary antibodies (Molecular Probes-Invitrogen) diluted at 1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... submitted to a Matrigel bath for 1h and media changed from neuroinduction media [1% N2 supplement (Gibco), 1% GlutaMAX (Life Technologies) ...
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bioRxiv - Neuroscience 2022Quote: ... at 200V for 1h for αBK and Bis-Tris 4-12% polyacrylamide gradient gels (Thermo Fisher NP0323) in MES-SDS running buffer (Thermo Fisher B0002 ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed HUVEC were blocked for 1h at RT in blocking solution (5% FBS, 2X antibiotic-antimycotic (Gibco), 0.1% sodium azide (s2002-100G ...
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bioRxiv - Molecular Biology 2023Quote: ... Separated proteins were transferred to nitrocellulose membrane (Amersham) 1h at 120V using 1x NuPAGE Transfer Buffer (Invitrogen) supplemented with 10% methanol ...
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bioRxiv - Microbiology 2022Quote: ... for 1h at RT following 30 min of incubation with secondary antibody (1:200, A11034, Life Technologies) and nuclei were visualized by using DAPI (Sigma Aldrich).
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bioRxiv - Molecular Biology 2023Quote: ... washed (4 x 300 µL PBST) and blocked for 1h at RT with 1% BSA (Fisher Scientific) in PBS solution ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody was detected after probing for 1h with HRP-linked rabbit anti-mouse IgG (AB_228307, Thermofisher) or goat anti-rabbit IgG (AB_228341 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked for 1h and probed with antibodies: BHLHE40 1:500 (Thermo Fisher Scientific, PA5-83044), BHLHE41 1:500 (Biorbyt ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... for 1h and then with Alexa Fluor® 488 or 647 secondary antibody (1/250, Life Technologies) supplemented with 2% donkey serum for 1h.
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bioRxiv - Genomics 2024Quote: ... Medium was furthermore supplemented with 250 nM Rapalog 1h before harvest and 50 µm Biotin (Invitrogen, B20656) 30 min before harvest ...
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bioRxiv - Molecular Biology 2024Quote: ... After overnight reaction at 37°C and 4U Turbo DNAse treatment 1h at 37°C (Invitrogen #AM2238), RNA molecules have been purified by TRIzol and Isopropanol precipitation ...
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bioRxiv - Cell Biology 2021Quote: ... and a mix of amino-acids (1X MEM Non-Essential Amino Acids and 1X MEM Amino Acids, both manufactured by Gibco R). For strains and plasmid construction cells were grown in LB ...
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bioRxiv - Cell Biology 2024Quote: ... a mix of amino acids (1× MEM Non-Essential Amino Acids and 1× MEM Essential Amino Acids, both manufactured by Gibco™) was added with the glucose ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed twice with 1X PBS and stained with either DAPI (4’,6-diamidino-2-phenylindole, from Life Technologies), for the visualization of the nucleus ...
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bioRxiv - Cell Biology 2020Quote: We seeded 2×105 cells / well into 6 well dishes and replaced media with 5% KSR (Knockout Serum Replacement) (Gibco) containing media the next day ...
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bioRxiv - Cell Biology 2020Quote: ... collected into a new tube and stained with 4′,6-diamidino-2-phenylindole (DAPI) (∼5 µg/ml, Molecular Probes # D1306). Round coverslips (Ø12 mm ...
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bioRxiv - Genetics 2021Quote: Live myofibers in the 6-well plate were stained with 2 µL of 5mg/mL of Hoechst (Molecular Probes, H1399) in 2 mL of 1X PBS for 5 minutes in a 37 °C with 5% CO2 incubator ...
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bioRxiv - Microbiology 2021Quote: ... Slides were washed for 3 × 5 mins in PBS after each antibody step and in the penultimate wash 2μg/ml 4’,6-diamidino-2-phenylindole (DAPI) (Molecular Probes) was added ...
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Therapy-induced lipid uptake and remodeling underpin ferroptosis hypersensitivity in prostate cancerbioRxiv - Cancer Biology 2020Quote: ... DNA and F-actin were counterstained for 20 min in the dark with 1 μL/ml 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen) and Alexa Fluor 647 phalloidin (Thermo Fisher) ...
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bioRxiv - Neuroscience 2020Quote: ... The coverslips were then mounted on slides using prolong Gold mounting medium with DAPI (4’,6-diamidino-2-phenylindole) (Invitrogen). RGCs immunohistochemistry was performed as described earlier 36–39 using SOX2 ...
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bioRxiv - Bioengineering 2020Quote: ... Primary fibroblast passages 2–6 were achieved in culture in 37°C in a 5% CO2 incubator with DMEM High Glucose (Gibco) with 10% FBS to 80% confluence in 100 mm Petri dishes ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed 5-6 times over 2 hours in PBT at room temperature and incubated with secondary antibodies (Invitrogen) diluted in NGS-PBT for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... in PBS for two hours at room temperature followed by 0.5 μg/mL of 4′,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific) in PBS for five minutes at room temperature prior to mounting.
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bioRxiv - Cell Biology 2021Quote: ... 2X SSC) for 5 min and counter stained with 1 μg/ml DAPI (4’,6-diamidino-2-phenylindole; Life Technologies). Coverslips were mounted in imaging buffer (3.7 μg/μl glucose oxidase and 1U catalase in equilibration buffer ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were washed three times with PBS and nuclei counterstained with 1 µg/ml 4’,6-diamidino-2-phenylindole (DAPI) (#D3571, Invitrogen) in PBS for 30 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... on 12 or 15 mm coverslips in a 6-well plate and 10 nM siRNA-1 or -2 was transfected using RNAi MAX reagents (Invitrogen) according the manufacturer protocol ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were counterstained with DAPI (4’,6-diamidino-2-phenylinodole) and mounted onto glass slides (Superfrost Plus; Fisher Scientific) with Vectashield mounting medium (H-1200 ...
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bioRxiv - Neuroscience 2021Quote: Hair cell nuclei were specifically labeled by briefly exposing free-swimming larvae to 4’,6-Diamidino-2-Phenylindole (DAPI; Invitrogen/ThermoFisher) diluted to 2.5 μg/ml in E3 ...