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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... brain sections were counterstained with the fluorescent nuclear dye 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Scientific, #D3571) and mounted with Mowiol (Polysciences ...
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bioRxiv - Pathology 2024Quote: ... Slides were embedded with Prolong Diamond Antifade Mountant containing 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific) to stain nuclei.
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bioRxiv - Bioengineering 2024Quote: ... Samples were washed in PBS and stained with 4’,6-diamidino-2-phenylindole (DAPI) (Thermo Scientific™, USA) for 5 minutes and Alexa 594-phalloidin (Invitrogen™ ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were again washed three times with PBS and mounted with 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen) for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... cell nuclei were stained with 1 µg 4’,6-Diamidino-2-Phenylindole (DAPI, Invitrogen, Thermo Fisher Scientific, D1306) for 15 min in a humidified incubator at 37 °C and 5% CO2 ...
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bioRxiv - Bioengineering 2024Quote: ... and cell nuclei were stained for 10 min with 4′,6- diamidino-2-phenylindole (DAPI; Thermo Fisher, USA). Finally ...
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bioRxiv - Cancer Biology 2024Quote: ... cell nuclei were stained with 1 µg 4’,6-Diamidino-2-Phenylindole (DAPI, Invitrogen, Thermo Fisher Scientific, D1306) for 15 min in a humidified incubator at 37 °C and 5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... The medium was changed every 2-3 days and organoids were passaged every 2-4 weeks by dissociation with 1 ml of TrypLE Express (Life Technologies) at 37°C for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... MAN0017058 by Invitrogen (pages 5 and 6). A non-targeting sgRNA that does not recognize any sequence in the human genome was used as a negative control (Invitrogen Cat# ...
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bioRxiv - Microbiology 2021Quote: ... 1 mL aliquots of the BLEB enrichments were serially diluted to 10-3 in phosphate buffered saline (PBS; 1M; potassium chloride [Fischer Chemical], potassium phosphate [Acros Organics] ...
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bioRxiv - Microbiology 2021Quote: ... and 40 µg/mL X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside; Thermo Scientific). Plates were incubated at room temperature for 48-72 h ...
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bioRxiv - Bioengineering 2020Quote: Before staining with either 5-bromo-4-chloro-3’-indolyphosphate and nitro-blue tetrazolium (BCIP/NBT, ThermoFisher) or Alizarin Red S (ARS ...
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bioRxiv - Microbiology 2024Quote: ... containing 100 mg/ml of X-gal (5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Thermofisher) to confirm CFU/ml counts.
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bioRxiv - Bioengineering 2020Quote: ... 2.5 μM of CellTracker™ Orange 5-(and-6)-(((4-chloromethyl)benzoyl)amino)tetramethyl-rhodamine CMTMR (Molecular Probes, C2927) was used to label iNeuron cells ...
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bioRxiv - Microbiology 2022Quote: ... A 4’-5-diamidina-2-phenylindole (DAPI)-containing mounting medium was used (ThermoFisher, cat. 62248). Images were acquired using a spectral Zeiss LSM800 confocal microscope or Confocal Zeiss LSM980 - AiryScan and analyzed with Fiji software (version 2.0.0) ...
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bioRxiv - Cell Biology 2021Quote: ... A 4’-5-diamidina-2-phenylindole (DAPI)-containing mounting medium was used (ThermoFisher, cat. 62248). Images were acquired using a spectral Zeiss LSM800 confocal microscope and analyzed with Fiji software (version 2.0.0) ...
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bioRxiv - Molecular Biology 2023Quote: ... FUCCI positive cells were cultured and washed once with phosphate-buffered saline (PBS, 300 x g / 5 min) and fixed with 4% methanol-free formaldehyde (Thermo Fisher Scientific, 28906) for 20 min at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... Each sample was incubated with 50 µm of 2’,7’-Bis-(2- carboxyethyl)-5-(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM; Molecular Probes, Eugene, OR) at 28°C ...
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bioRxiv - Cell Biology 2023Quote: ... or 5’-TCAAGGTCCCTGATAATTATGGCGA-3’) or scrambled siRNA (non-targeting control) using 6 μL Lipofectamine™ RNAiMAX (Invitrogen™ - Cat.# 13778075). After 24 h ...
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bioRxiv - Biophysics 2023Quote: ... 7-Diethylamino-3-(4’-Maleimidylphenyl)-4-Methylcoumarin (CPM) (Invitrogen. USA), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC ...
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bioRxiv - Bioengineering 2020Quote: ... 4’-6-diamidino-1-phenylindole (DAPI, Life Technologies) was applied at 1 μg/mL for 90 minutes to stain the nuclei and the samples were washed and mounted with AquaPoly/Mount (Polysciences) ...
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bioRxiv - Microbiology 2022Quote: ... 300 μl of the induced samples were centrifuged at 8,000xg for 2 minutes at 25 °C and resuspended in 5 μl of Dulbecco’s phosphate-buffered saline (DPBS) (Thermo Fisher Scientific 14200075), supplemented with 1 mg/ml membrane stain FM1-43 for bacteria and FM4-64 for yeast (Thermo Fisher Scientific T35356 and T13320 ...
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bioRxiv - Cell Biology 2024Quote: ... Recombinant wt and mutant αSyn were added to freshly prepared and sterile filtered electroporation buffer (EPB, 100 mM sodium phosphate, 5 mM KCl, 15 mM MgCl2, 15 mM HEPES, 2 mM ATP (Thermo Fisher #R0441), 2 mM reduced glutathione (Sigma # G4251 ...
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bioRxiv - Microbiology 2021Quote: ... 40 U/ml creatine phosphokinase and 4 mM creatine phosphate (Thermo Scientific), 2% (w/w ...
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bioRxiv - Neuroscience 2022Quote: ... cells were washed three times with 4°C phosphate-buffered saline (Gibco) and sulfosuccinimidyl-2-(biotinamido)ethyl-1,3-dithiopropionate-biotin (sulfo-NHS-SS-biotin ...
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bioRxiv - Microbiology 2021Quote: ... Paraformaldehyde (4% final concentration) in 1x phosphate buffered saline (PBS, Invitrogen, 10010023) was then added to the plates to fix the cells ...
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bioRxiv - Neuroscience 2024Quote: ... followed by cold 10% phosphate buffered formalin (#SF100-4, Thermo Fisher Scientific) for fixation ...
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bioRxiv - Bioengineering 2024Quote: ... and a lentiviral transfer plasmid (2:3:4 ratio by mass, 3 µg total plasmid) using Lipofectamine 3000 (Thermo Scientific cat. no. L3000015). Media was exchanged after 6 hours and viral supernatant was harvested 48 hours after transfection and filtered through 0.45 μm cellulose-acetate filters (VWR cat ...
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bioRxiv - Cell Biology 2022Quote: ... Guide RNA primer sets A (KS40 5’-ACCGACCACAGAAACTGCGACTAG -3’ and KS41 5’-AACCTAGTCGCAGTTTCTGTGGTC-3’) and B (KS42 5’-CCGTCCCACTGGCACCGCTTTATG-3’ and KS43 5’-AAACATAAAGCGGTGCCAGTGGGA-3’) were phosphorylated with T4 polynucleotide kinase (ThermoFisher) at 37°C for 30 min and annealed by cooling down from 85°C to 25°C at 0.1°C/sec ...
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bioRxiv - Neuroscience 2021Quote: ... DiIC18(3) dye (6 mg; Invitrogen, Carlsbad, CA, USA) was dissolved in 99.5% methylene chloride (300 μL ...
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bioRxiv - Biochemistry 2023Quote: ... NaHCO3 and 150 μL acetone containing 4 mg/mL 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide (L-FDAA, Thermo Scientific) was added ...
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bioRxiv - Developmental Biology 2022Quote: ... After 3 washes with Ca2+-Mg2+ free Dulbecco Phosphate Buffered Saline (DPBS, Gibco), wells were blocked with 5% non-fat dry milk in Tris Buffered Saline (TBS ...
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bioRxiv - Cell Biology 2020Quote: ... VIM (Hs00958111_m1) and glyceraldeyde-3-phosphate dehydrogenase (GAPDH) (Hs02758991_g1) were purchased from ThermoFisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and rinsed 3 times with Dulbecco’s phosphate buffer saline (D-PBS) (Thermo Fisher Scientific ...
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MK2 deficiency decreases mortality during the inflammatory phase after myocardial infarction in micebioRxiv - Physiology 2023Quote: ... Mouse monoclonal antibodies against GAPDH (glyceraldehyde 3-phosphate dehydrogenase) (# 4300) were from Ambion. Secondary antibodies conjugated with horseradish peroxidase were from Jackson ImmunoResearch Laboratories ...
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bioRxiv - Immunology 2023Quote: ... A rodent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) primer and probe set (Applied Biosystems) were included in each reaction to normalize the DNA added ...
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bioRxiv - Microbiology 2023Quote: ... the cellular glyceraldehyde 3-phosphate dehydrogenase (GADPH) with SuperScriptTM III Reverse Transcriptase (Invitrogen) and quantified using SYBR-Green (Roche ...
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bioRxiv - Genomics 2024Quote: ... 3) We did the optional wash with Phosphate Buffered Saline (PBS; Invitrogen, AM9625) containing 0.01% BSA (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... we used 15 μL of a mixture of 4’,6-diamidin-2-phenylindole (4 μg mL−1) in SlowFade Gold Antifade Mounting medium (both Thermo Fisher Scientific, Waltham, MA, USA). All solutions and buffers for virus-targeted genomeFISH experiments were prepared with molecular grade water (Carl Roth ...
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bioRxiv - Immunology 2021Quote: ... Samples from Biosafety Level 3 were inactivated for 2 hours at RT with 4% paraformaldehyde (ThermoFisher Scientific) after extracellular and intracellular staining.
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bioRxiv - Microbiology 2022Quote: ... adding 3 mL HPLC grade methanol and 2 mL HPLC grade chloroform (C297-4, Thermo Fisher Scientific), then shaking at 22°C for 1 hr ...
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bioRxiv - Genomics 2023Quote: ... and CRISPRmap library plasmid (2:3:4 ratio by mass) using Lipofectamine 3000 (Thermo Fisher Scientific L3000001) in lentiviral packaging medium (Opti-MEM (Thermo Fisher Scientific 31-985-070 ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were mounted on ProLong Gold with 4’,6’-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific, Waltham, MA, USA) and imaged using a Delta-Vision II microscope system with an Olympus IX-71 inverted microscope using a 100x objective A ...
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bioRxiv - Molecular Biology 2020Quote: ... Slides were mounted in ProLong™ Gold Antifade Mountant containing 4’,6’-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific). Images were acquired at 100x magnification using a Nikon Eclipse Ti fluorescence microscope fitted with a Hamamatsu C11440 digital camera ...
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bioRxiv - Microbiology 2020Quote: ... Finally samples were counter stained for nucleus with 1 μM DAPI (4′, 6-diamidino-2′-phenylindoldihydrochloride; Thermo Fisher Scientific). Zeiss inverted Axio Observer fluorescent microscope (Zeiss ...
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bioRxiv - Immunology 2021Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole and were embedded with ProLong Gold mounting medium (Life Technologies). ImageJ/Fiji software (National Institutes of Health ...
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bioRxiv - Neuroscience 2020Quote: Human sections were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) and coverslips were mounted using Prolong Gold (Invitrogen) for imaging ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were mounted in ProLong Gold antifade reagent supplemented with 4′,6-diamidino-2-phenylindole (DAPI; Molecular Probes). Conventional immunocytochemical staining was done to quantify the activated PAK1 and actin in oAβ and IPA-3 treated cells using phospho-PAK1 (Thr423)/PAK2 (Thr402 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Chromosomes were counterstained for 20 minutes with 1 μg/ml DAPI (4’,6-diamidino-2-phenylindole; ThermoFisher Scientific, D3571) in 2x SSC ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then washed twice with PBS and stained with 4′,6-diamidino-2-phenylindole (DAPI, Thermo Scientific #D1306) diluted 1:200 in 0.5% BSA in PBS ...