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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Samples were mildly fixed with 4% PFA for 4 min before immunostaining using anti SARS-CoV-2 Spike Protein S1 (Cat# PA5-114447, ThermoFisher Scientific) and anti-human IgG (Fc)-Alexa 488 (Cat# 109-545-170 ...
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bioRxiv - Neuroscience 2024Quote: ... sham and uPA-CBP were anesthetized (4% isoflurane, 2 liters of oxygen) and transcardial perfusion fixed with 4% paraformaldehyde (PFA) (Cat #50-980-494, Fisher Scientific) in PBS 1x until stiff ...
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bioRxiv - Developmental Biology 2024Quote: ... with a Dionex IonPac AG11-HC-4 μm guard column (2 mm i.d × 50 mm, 4 μm particle size, Thermo Fisher Scientific) and a Dionex IonPac AS11-HC-4 μm column (2 mm i.d × 250 mm ...
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bioRxiv - Developmental Biology 2024Quote: ... and a Dionex IonPac AS11-HC-4 μm column (2 mm i.d × 250 mm, 4 μm particle size, Thermo Fisher Scientific) coupled to a Q Exactive mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... with 4 μg of a plasmid mixture (1:1:4 THR:RXR:TRE-Luc) in 200 μL Opti-MEM (Invitrogen, 11058-021) and added to the cells ...
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bioRxiv - Cell Biology 2021Quote: ... Rev: 5’-TCATTGAGACACCATTTGTC-3’ were cloned into pCR™4-TOPO® TA vector using the TOPO-TA cloning kit (Thermo Fisher 450030) and sequence verified.
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bioRxiv - Cell Biology 2024Quote: ... or luciferase siRNA (5’ CGUACGCGGAAUACUUCGA 3’, control) were transfected in above RPE1 cells using 4 µl of lipofectamine siRNAmax (Thermo Fisher Scientific, 13778075) and 10 µM of siRNA in 2 ml of cell culture media ...
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bioRxiv - Microbiology 2022Quote: ... incubated for 10 min in the dark at room temperature with FM 4-64FX (3 μg ml-1) (ThermoFisher). Cells were applied to a 5% agarose pad containing M9 minimal media with glucose (0.4%) ...
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bioRxiv - Genetics 2022Quote: Immunoprecipitations were performed using 1 μg of Anti-Trimethyl-Histone-3-Lys-4 (-Thermo Fisher Scientific; catalog# PA5-17420) overnight at 4 ◦ C ...
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bioRxiv - Neuroscience 2022Quote: 100 to 150 3-dpf zebrafish larvae were bathed in Yo-Pro-1 (4 µM, 30 min) (Invitrogen, Y3603), then washed 3 times in blue water to label lateral line hair cells ...
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bioRxiv - Immunology 2023Quote: ... and packaging vector psPAX2 were mixed in a 4:3:1 ratio in OPTI-MEM (Thermo Fisher Scientific, 31985070) and PEI (Polysciences ...
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bioRxiv - Molecular Biology 2020Quote: ... siRNA (4): s35234 and siRNA (5): s35235 (Silencer Select, ThermoFisher Scientific, USA); EMC2 s18670 and EMC5 s41131 (Silencer Select ...
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bioRxiv - Immunology 2021Quote: ... 2.5-5 µg was loaded onto a NuPAGE 4-12% gel (ThermoFisher) and run at 100V for 2-3 h ...
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bioRxiv - Bioengineering 2023Quote: Samples were loaded with 5 μM Fluo-4 AM (ThermoFisher Scientific, Germany) in CM for 30 min in a cell culture incubator (37 °C and humidified 5% CO2) ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 ng/ml IL-4 (Thermo Fisher Scientific, Cat#14-8041-62) and 1 ng/ml TGF-β1 (Cell Signaling Technology ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... After 4-5 minutes 30 µM SCH-23390 (Fisher Scientific Cat#: 092550) in ACSF was applied for 1 minute ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and passaged every 4-5 days using StemPro Accutase (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2024Quote: ... and incubated overnight at 4° C with 5 mM MANT-GDP (Invitrogen). Excess nucleotide was removed using a GE FPLC using a HighPrep 26/10 column into a buffer containing 20 mM HEPES (pH 8.0) ...
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bioRxiv - Cell Biology 2024Quote: ... 4-5×106 cells were resuspended in 100 μL of DMEM (Invitrogen) and 4 μg of plasmid DNA ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were centrifuged at 17,800 × g for 5 min at 4°C and supernatants were mixed with NuPAGE LDS Sample Buffer (4×) buffer (Thermo Fisher Scientific) containing 1% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were centrifuged at 20,800 × g for 5 min at 4°C and supernatants were mixed with NuPAGE LDS Sample Buffer (4×) buffer (Thermo Fisher Scientific). For SDS-PAGE ...
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bioRxiv - Immunology 2024Quote: 50 μg exosomes were incubated with 5 μm aldehyde/sulfate latex beads (4% w/v, 4 µm, Invitrogen™, Massachusetts, United States) in a 100 μl final volume of DPBS and incubated for 15 min on a shaker at RT ...
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bioRxiv - Biochemistry 2020Quote: ... and 4 μg ml−1 Blasticidin (Thermo Fisher), single cell colonies were collected and subsequently expanded ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-Ki67 (ThermoFisher, 4-5698-82, 1:100), anti-Actinin (Cell Signaling ...
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bioRxiv - Bioengineering 2020Quote: ... 4’-6-diamidino-1-phenylindole (DAPI, Life Technologies) was applied at 1 μg/mL for 90 minutes to stain the nuclei and the samples were washed and mounted with AquaPoly/Mount (Polysciences) ...
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bioRxiv - Cell Biology 2022Quote: ... ERCC 1:4 × 107 dilution (Ambion, cat: 4456740) in 384-well plates prior to library preparation.
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bioRxiv - Genomics 2021Quote: ... 1 mL of hexane (Fisher Scientific; H303-4) followed by 1 mL of deionized water was added to each sample ...
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bioRxiv - Genomics 2023Quote: ... 4 µL DNase I (ThermoFisher, 1 unit/µL), 4 µL RNase A (ThermoFisher ...
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bioRxiv - Bioengineering 2024Quote: ... and 4 μM ethidum homodimer-1 (Invitrogen™) in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-spectrin beta-4 (1:100, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... 4 μM ethidium homodimer-1 (EthD1, ThermoFisher, E1169), and 0.02 mg/ml deoxyribonuclease I ...
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bioRxiv - Bioengineering 2024Quote: ... and 4 μM Ethidum homodimer-1 (Invitrogen™) in PBS for 30 min at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... anti-claudin 4 (1:100, Invitrogen 36-4800), anti-laminin (1:250 ...
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bioRxiv - Cell Biology 2024Quote: ... diluted 1:4 in 1X DPBS (ThermoFisher, 14190144), 0.05 μL of 40 μM ASO diluted in 4.165 μL Opti-MEM was combined with 4.165 μL Lipofectamine RNAiMAX diluted in 0.25 μL Opti-MEM ...
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bioRxiv - Cell Biology 2024Quote: ... diluted 1:4 in 1X DPBS (ThermoFisher, 14190144). All 5eU-seq experiments were performed on MCF10A cells.
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bioRxiv - Plant Biology 2022Quote: ... A second round of reverse transcription was initiated with the addition of 4 µL of reverse transcription mix (4:2:1:1 of First Strand Buffer, 0.1 M DTT, RNaseOUT, SuperScript II; ThermoFisher Scientific) to each sample then incubated at 25□ for 10 min ...
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bioRxiv - Genomics 2022Quote: ... resuspended in 1 mL NSB with 1:20 dilution of 0.25 mg/ml 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen D1306) and FACS sorted ...
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bioRxiv - Biophysics 2023Quote: ... the samples were added with a DNA-binding dye 4’,6-diamidino-2-phenylindole (DAPI, 1 μg mL-1 in PBS, Invitrogen) along with the secondary antibody solution ...
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bioRxiv - Bioengineering 2023Quote: ... RPMI 1640 containing 2 μM calcein AM and 4 μM EthD-1 along with 1 mg/mL DAPI (ThermoFisher, 62248) was added to the 384-deep well plate (80 μL/well) ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were added with a DNA-binding dye 4′,6-diamidino-2-phenylindole (DAPI, 1 µg mL-1 in PBS, Invitrogen) and phalloidin conjugated Alexa Fluor dye (Invitrogen ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA labeling was performed using PBS containing 1 µg/ml 4′,6-diamidino-2-phenylindole (DAPI) (1:10000) (Invitrogen, D1306). After 3 additional washes with PBS ...
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bioRxiv - Bioengineering 2024Quote: ... and 4 µM ethidium homodimer-1 (EthD-1, Invitrogen, CA) for 45 min in an incubator and then imaged using the LSM 880 Zeiss confocal microscope ...
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bioRxiv - Biochemistry 2024Quote: ... with 4 µM Ethidium-Homodimer 1 (EthD-1) (Thermofisher, #E1169) and 2 µM Calcein-AM ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was mixed in a 4:1 ratio with 4-methylvaleric acid (Thermo Fisher catalogue number AAA1540506) in 6% v/v phosphoric acid and copper sulfate pentahydrate ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed 4 × 5 min with PBS before incubating with secondary antibodies (1:1000 Alexa fluorophores, ThermoFisher) in blocking solution for 1 hr at room temperature ...
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In vivo imaging of the kinetics of microglial self-renewal and maturation in the adult visual cortexbioRxiv - Neuroscience 2020Quote: ... clone G-A-5) followed by a secondary antibody solution (4 h, RT, AlexaFluor 594, 1:500, Invitrogen), mounted and coverslipped ...
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bioRxiv - Developmental Biology 2022Quote: ... centrifuged at 500xg for 5 min at 4°C and resuspended in 1% BSA in PBS (AM2616, Invitrogen) containing 200 U/μl RNase inhibitor (3335402001 ...
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bioRxiv - Physiology 2022Quote: ... the fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG; 6.83 μg/kg BW, Invitrogen) dissolved in 50% dextrose (1 g/kg BW ...
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bioRxiv - Immunology 2024Quote: ... Cells were then incubated for 30min at 37°C with 150µM of 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose (ThermoFisher).
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bioRxiv - Immunology 2021Quote: ... was added as the secondary antibody at a 1:2000 dilution for 1 h at 37C, followed by adding TMB (3, 3, 5, 5’-tetramethylbenzidine) peroxidase substrate (Thermo Scientific) for about 15 min ...