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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... A 4:1 composite medium of Dulbecco’s minimum essential medium (DMEM, Gibco, Invitrogen, NY, USA) and Medium 199 ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissues were then stained with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, D1306, 1:5000) for five minutes to visualize DNA ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated in 4′,6-diamidino-2-phenylindole (DAPI) (1:1000) (Invitrogen, Carlsbad, CA, USA) for 10 minutes and washed with PBS again ...
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bioRxiv - Biochemistry 2022Quote: ... for 1 hour at 4°C and analyzed on an Attune Flow Cytometer (ThermoFisher Scientific).
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bioRxiv - Bioengineering 2023Quote: ... cell media was removed and 1 ml of 4 µm Calcein AM stain (Fisher Scientific) in PBS was added before being left to incubate for 30 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were then counterstained with 4’,6-diamidino-2-phenylindole (DAPI) (D1306, 1:10,000, Invitrogen) diluted in 1X PBST (1:10,000) ...
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bioRxiv - Bioengineering 2023Quote: ... we add 1 mL of 4% w/v paraformaldehyde (Thermo Scientific, Cat. No. AA47392-9M) to the apical side of the Transwell® and incubate the culture at room temperature (RT ...
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bioRxiv - Bioengineering 2023Quote: ... followed by incubation with 2 μM calcein-AM and 4 μM ethidium homodimer-1 (Invitrogen) for 30 min to examine viability ...
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bioRxiv - Bioengineering 2023Quote: ... 1% penicillin-streptomycin and 4 mM L-glutamine (all three from Gibco, Thermo Fisher Scientific). Cells were plated at a density of 120,000 per dish either on high precision #1.5 25 mm coverslips (Marienfeld ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 4 mg ml-1 collagenase IV (Thermo Fisher Scientific, Catalog no. 17104-019) and DNAse I (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... 1% penicillin-streptomycin and 4 mM L-glutamine (all three from Gibco, Thermo Fisher Scientific). Cells were plated at a density of 120,000 per dish either on high precision #1.5 25 mm coverslips (Marienfeld ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were seeded in a 4-chamber glass bottom dish (D35C4-20-1-N, Invitrogen) for confocal imaging or in a 60 mm × 15 mm dish (705001 ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1-20 µg of protein was loaded onto 4-12% NuPage BisTris gels (Invitrogen) for electrophoresis ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei were stained with DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate, 1:3000; #D3571, Invitrogen) then mounted on glass slides with Mowiol ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... PBS (pH 7.4) containing 2X ethidium homodimer-1 (4 μM; #L3224B, Thermo Fisher Scientific, USA) was added to the existing medium in each well at a 1:1 ratio ...
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bioRxiv - Microbiology 2024Quote: ... AF647-conjugated donkey anti-sheep IgG (Thermo Fisher, A-21448, 1:500, 4 µg/mL), CF568-conjugated donkey anti-goat IgG (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... AF647-conjugated donkey anti-rabbit IgG (Thermo Fisher, A-31573, 1:500, 4 µg/mL), AF647-conjugated donkey anti-sheep IgG (Thermo Fisher ...
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bioRxiv - Systems Biology 2024Quote: ... diluted with Advanced DMEM/F12 medium using a 4:1 ratio (Thermofisher Scientific, Cat # 12634) in Corning™ 3527 24-well plates (VWR International Gmbh ...
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bioRxiv - Bioengineering 2024Quote: ... and Alexa Fluor 647 (4 µg/mL, A21244, Invitrogen and 1:500, #4410, Cell Signaling). To stain for nucleus and cytoskeleton ...
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bioRxiv - Molecular Biology 2024Quote: ... and 4’,6-diamidino-2-phenylindole (DAPI) for nuclear staining (H3570; Invitrogen; dilution 1:200), diluted in phosphate-buffered saline (PBS) ...
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bioRxiv - Immunology 2024Quote: ... Cells were resuspended in 100µL of 1:4 Fixation/Permeabilization concentrate (Invitrogen 00-5123-43) in Fixation/Permeabilization diluent (Invitrogen 00-5223-56 ...
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bioRxiv - Biophysics 2024Quote: ... to visualize actin filaments and with 4’-6-diamidino-2-phenylindole (DAPI, Invitrogen, 1:200) for the nucleus ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated with primary antibody 4-HNE-adduct antibody (Invitrogen, #MA5-27570, 1:500) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... slices were fixed for 1 h in PBS with 4% PFA and Streptavidin conjugated to Alexa 647 (1:500, ThermoFisher Scientific) was applied during secondary incubation following blocking and permeabilization.
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bioRxiv - Immunology 2021Quote: 293T cells were co-transfected with pHDM-Wuhan-Hu-1 and pMAX-GFP plasmids at a 4:1 ratio by lipofectamine 3000 (Thermo fisher). 293T cell pool cotransfected with pHEF-VsVg and pMAX-GFP plasmids at a 4:1 ratio was used as a negative control ...
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bioRxiv - Cell Biology 2020Quote: ... the pooled anion exchange fractions were phosphorylated in vitro overnight at 4 °C with 1 mM ATP and 1 μg of PKR(252-551)-GST enzyme (Thermo Scientific) per mg of eIF2α ...
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bioRxiv - Immunology 2021Quote: ... 5×105 murine or human T cells have been stimulated at 1:1 ratio in 48well plate in presence of Sulfate Latex 4% w/v 5µm Beads (Molecular Probes S37227). For each experiment ...
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bioRxiv - Microbiology 2021Quote: ... antibody 1:3000 in 1% PBS/BSA at 4°C followed by secondary antibody conjugated to Alexa Fluor 555 (Thermo Fisher) at 1:500 in PBS/BSA 1% was performed ...
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bioRxiv - Biochemistry 2022Quote: ... pH 7.4/50 mM KOAc/2 mM EDTA, pH 8/1 mM DTT/1 mM PMSF/1x Phosphatase inhibitor cocktail, Thermo Scientific), 0.3 g of glass beads (acid washed ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with primary antibody (D1R: anti-D1R [mouse] 1:1500, Novus Biologicals, 48 hrs at 4°C; Th: anti-Th [rabbit] 1:1000, ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was brought up to 30 mL with Buffer K and nutated for 1 hr at 4°C with 1 mL bed volume of Buffer K-equilibrated His-Pur cobalt resin (Thermo Scientific), adding 30 uL fresh PI ...
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bioRxiv - Microbiology 2021Quote: ... and incubated with primary antibodies (1% BSA/PBS-T, overnight at 4°C) at the following dilutions: mouse anti-SAG1 (1:1000, Thermo Scientific) and rat anti-HA (1:1000 ...
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bioRxiv - Physiology 2020Quote: ... primary adipocytes were attached to poly-L-lysine (25-100 μg ml-1) coated coverslips and incubated with the Ca2+ fluorophore Fluo-4 AM (1 μM; Molecular Probes) in modified Hank’s solution for 1 hour at 21-23°C in the dark ...
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bioRxiv - Biochemistry 2020Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes).
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was removed and the RPE sheets/fragments were resuspended in 4 ml RPE media (1:1, DMEM3: Ham’s F12 medium (GIBCO 31330-038), 10% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... samples were washed in block six times for 1 hour and incubated in secondary antibodies conjugated to fluorescent dyes diluted 1:250 in block with added 4’,6-diamidino-2-phenylindole (DAPI; 1:500 dilution of 1 mg/ml stock, Thermo Fisher) for 2 nights at 4°C.
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bioRxiv - Cell Biology 2022Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes). Cells were imaged using a DeltaVision Ultra microscope with a 60X objective (NA = 1.42) ...
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bioRxiv - Neuroscience 2022Quote: ... Whole muscles were washed in 0.3% PBST for 5 hours then incubated in secondary antibodies for 48h at 4°C on rotating incubator (AlexaFluor 568, 1:200, Invitrogen, A11041; AlexaFluor 647, 1:200, Invitrogen, A21236). Whole muscles were dehydrated in a series of 25% ...
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bioRxiv - Cancer Biology 2022Quote: ... at 1:250 dilution overnight at 4°C and anti-mouse AlexaFluor-555 secondary antibody (1:1000, ThermoFisher Scientific, #A-21424) for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... cDNA was diluted 1:3 or 1:4 before mixing with Power SYBR Green PCR Master Mix (CAS: 4368702, Applied Biosystems) and primer pairs (Table S4B) ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated at 4°C for 18 hours with primary antibodies: E-Cad (13-1700, Thermo Fisher, HECD-1, 1:100) and CK-14 (RB-9020 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Lot 134874), and 4-(2-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, purity ≥ 99.%, CAT #BP310-1, Lot 052975) were from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2024Quote: ... 1:600) antibody was incubated at 4°C overnight followed by secondary antibody anti-rabbit Alexa 488 (ThermoFisher Scientific, 1:600) for 1h at RT ...
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bioRxiv - Cell Biology 2023Quote: ... Frozen rat ovarian follicles were homogenized in 400 µL lysis buffer (100 mM HEPES, pH 8, 4% SDS, 1 mM EDTA, and 1× Halt protease/phosphatase inhibitor cocktail (ThermoFisher Scientific) using Zirconia/glass beads in a bead beating grinder (FastPrep24 ...
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bioRxiv - Immunology 2023Quote: ... 20 ng ml-1 GM-CSF (Biozym) or X38-Ag8 supernatant (1%) and from day 5 on additionally with 10 ng ml-1 IL-4 (Life technologies). At day 7 ...
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bioRxiv - Biophysics 2023Quote: ... blocked and imaged as described under section triple-color immunostainings but were incubated with mouse anti-AnkG (#AB_10675130, Neuromab, 1:200) overnight at 4°C and subsequently with donkey anti-mouse AF647 (#A31571, Invitrogen, 1:800) for 1h.
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bioRxiv - Molecular Biology 2023Quote: ... or anti-NIPBL rabbit antibodies followed by 1 h incubation at 4 °C on a rotating wheel with 40 μl of a 1:1 mix of Dynabead protein A (10001D, Life Technologies) and G (10003D ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were washed and suspended with 1 mL of 4% FBS/PBS containing DAPI (1:2000; Thermo Fisher Scientific, 62248) and DRAQ5 (1:5000 ...
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bioRxiv - Biochemistry 2023Quote: ... containing 1 mM EDTA and 1 mM dithiothreitol were reacted with a 4-fold concentration of Protein Thermal Shift Dye (Life Technologies) in a 20 μl reaction volume ...
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bioRxiv - Neuroscience 2023Quote: ... 4) with secondary Abs anti-mouse or rabbit IgG conjugated Alexa 488 (1:400) or Alexa 568 (1:200) (Life Technologies).