Labshake search
Citations for Click Chemistry Tools :
1 - 50 of 50 citations for Semicarbazide Hydrochloride Sem 13C 99%; 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... and 100 μg/mL cycloheximide and incubated with 100 μM L-azidohomoalanine (AHA; Click Chemistry Tools) and 10 μM methionine-free amino acid mixture (Promega) ...
-
bioRxiv - Molecular Biology 2024Quote: ... 25 µl of methanol-preserved cells (∼30’000 cells in total) were mixed with 1 mM NHS-TCO (Click Chemistry Tools) and incubated in the dark at 21 °C for 5 minutes ...
-
bioRxiv - Cell Biology 2024Quote: ... BTTAA (Click chemistry tools, 1236-100); CalFluor 647 Azide(Click chemistry tools ...
-
bioRxiv - Molecular Biology 2022Quote: ... 2 mM THPTA (Click Chemistry Tools 1010-100), and 100 µM copper sulfate (Fisher Scientific AC197722500) ...
-
bioRxiv - Biochemistry 2021Quote: ... 100 μM BTTAA (Click Chemistry Tools, #1236-500), 50 μM CuSO4 and 2.5mM freshly prepared sodium ascorbate at 25°C for 2 h ...
-
bioRxiv - Biochemistry 2022Quote: ... 100 uM Biotin-Azide (1167-5, Click Chemistry Tools) and incubated for another 1 h ...
-
bioRxiv - Genomics 2022Quote: 100 µl of 100 µM oligo barcode (IDT) in PBS and 10 µl of 10 mM azide-NHS (Click Chemistry Tools cat# 1251-5) in DMSO were mixed at room temperature for two hours ...
-
bioRxiv - Systems Biology 2020Quote: ... SEAC were incubated with EdU (1149-100, Click Chemistry Tools) the night before for 12 hours ...
-
bioRxiv - Immunology 2022Quote: ... DBCO-PEG4-NHS (Click chemistry tools, 100 mM in DMSO) and dye-NHS (10 mM in DMSO ...
-
bioRxiv - Cell Biology 2023Quote: ... 5 μL of 100 mM BTTAA (Click Chemistry Tools, Scottsdale, AZ) in DMSO ...
-
bioRxiv - Biochemistry 2022Quote: ... and 100× molar equivalents of DBCO-AF647 (Click Chemistry Tools, 1302-1) in DMSO were added and the reaction was mixed at 500 rpm in the dark for 2 hours rt ...
-
bioRxiv - Molecular Biology 2020Quote: ... The lysates were reacted with 100 μM azide-PEG3-biotin (Click Chemistry Tools), 500 μM CuSO4 ...
-
bioRxiv - Bioengineering 2022Quote: ... poly(ethylene glycol) methyl ether DBCO (20 kDa, A120-100, Click Chemistry Tools), poly(ethylene glycol ...
-
bioRxiv - Microbiology 2021Quote: ... Samples were subsequently incubated with 100 μM diazo biotin azide (azo-tag; Click Chemistry Tools), 1 mM tris(2-carboxyethyl)phosphine (TCEP ...
-
bioRxiv - Microbiology 2021Quote: ... 15 mL portions of each sample were resuspended in 15 mL of incubation media amended with 1 mM homopropargylglycine (HPG; Click Chemistry Tools, USA) (HPG-positive microcosm) ...
-
bioRxiv - Immunology 2020Quote: ... 8 μL of 100 mM TCO-PEG4-NHS in DMSO (10 mM final; Click Chemistry Tools, A137), pH 8.5 was rotated at room temperature for 15 min ...
-
bioRxiv - Biophysics 2023Quote: ... 50ul of 1mg/ml of BSA-Azide (Click chemistry tools, 1535) was incubated with 15ul of 100uM (5’ ...
-
bioRxiv - Microbiology 2023Quote: ... Bacteria were pulse-labeled for 60 min by supplementing 1 mM L-AHA (Click Chemistry Tools, 1066-100) or L-methionine (Sigma ...
-
bioRxiv - Cell Biology 2023Quote: ... were dissolved in dry DMF (100 µL) in a plastic screw cap tube and 3-azidopropyl-1-amine (3.7 μL, 37.2 μmol, Click Chemistry Tools) followed by DIEA (13 μL ...
-
bioRxiv - Cell Biology 2020Quote: ... via copper(I)-catalyzed azide-alkyne 1,3-dipolar cycloaddition (CuAAC) “click” reaction as follows: 100 mM CuSO4 and 50 mM BTTAA (#1236, Click Chemistry Tools) were premixed at 1:5 ratio ...
-
bioRxiv - Bioengineering 2022Quote: ... 30 μL of 200 μM amine-ended biotinylated oligos was incubated with 100 mM photocleavable DBCO-NHS ester (Click Chemistry Tools) at 1 ...
-
bioRxiv - Bioengineering 2021Quote: ... while 30 μL of 200 μM cDNAs were incubated with 1.2 μL of 100 mM UV-cleavable DBCO-NHS ester (20 equivalents to cDNAs; Click Chemistry Tools) in 30% DMF for 4 h ...
-
bioRxiv - Neuroscience 2022Quote: ... adherent cells or tissue sections were permeabilized for 20 minutes in 0.5% Triton X-100 and incubated in the dark for 30 minutes with copper-dependent click reaction mixture (Click Chemistry Tools #1350) to label EdU with Azide-biotin (Click Chemistry Tools #1265 ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were permeabilized for 20 minutes in 0.5% Triton X-100 and incubated in the dark for 30 minutes with copper-dependent click reaction mixture (Click Chemistry Tools #1350) to label EdU directly with fluorophores (Azide-488 or –647) ...
-
bioRxiv - Biochemistry 2023Quote: ... the substrate was diluted to 100 μM and incubated for 10 minutes at room temperature with 200 μM SulfoCy5 (for stopped-flow measurements, Click Chemistry Tools) or LD555 (for smFRET measurements) ...
-
bioRxiv - Developmental Biology 2022Quote: ... The click reaction was performed for 1 hour in click staining solution (100 mM Tris pH 8.5, 1 mM CuSO4, 2 mM AZDye-488 Azide (Click Chemistry Tools, 1275-1), and 100 mM ascorbic acid) ...
-
bioRxiv - Biophysics 2023Quote: ... Azide-PEG4-NHS ester (Cat# AZ103-100) and Sulfo-DBCO NHS ester (Cat# A124-10) were purchased from Click Chemistry Tools (Scottsdale, AZ). Alexa 488 Mouse Anti-ZAP70 (pY319)/Syk (Y352 ...
-
bioRxiv - Genetics 2024Quote: ... permeabilized with 0.5% TritonX-100 for 15 minutes at room temperature and subjected to click labeling reaction using AFDye 488 Azide (Click Chemistry Tools, #1314). The AFDye 488-labeled cells were analyzed by flow cytometry using the FITC channel.
-
bioRxiv - Bioengineering 2024Quote: ... dicysteines resuspended at a 40 mM concentration were prereacted with 100 µM of AlexaFluor 488 maleimide (1:400 dye:peptide) (Click Chemistry Tools; Scottsdale, AZ) for 15 minutes ...
-
bioRxiv - Molecular Biology 2021Quote: The Click reaction was then performed by incubating cells with 100 µL Click buffer (100 µM copper sulfate, 2 mM THPTA, 10 mM fresh sodium ascorbate, 10 µM Cy5 picolyl azide (Click Chemistry Tools 1171-1)) for 1 hour at 37°C in the dark ...
-
bioRxiv - Biochemistry 2020Quote: ... Lysates containing 50 μg of protein were then incubated for 2 h with shaking at 37°C with 100 μM TAMRA azide (Click Chemistry Tools Scottsdale, Az), 800 μM copper(II ...
-
bioRxiv - Cell Biology 2022Quote: ... were transfected with pcDNA3.1-SARS CoV-2-Spike-C9 and incubated overnight in DMEM supplemented with 10% (v/v) charcoal-stripped FBS with or without 100 μM of clickable fatty acid analogs (Click Chemistry Tools, Scottsdale, AZ) coupled to albumin ...
-
bioRxiv - Molecular Biology 2022Quote: ... in situ click reactions were performed by incubating cells with 100 µL Click buffer (100 µM copper sulfate, 2 mM THPTA, 10 mM fresh sodium ascorbate, 10 µM Cy3 picolyl azide (Click Chemistry Tools 1178-1)) for 1 hour at 37°C in the dark ...
-
bioRxiv - Biochemistry 2023Quote: ... 1 g lysate (500 µL) were reacted with click reagents to give final concentrations as follows: 100 µM of diazo biotin-azide (Click Chemistry Tools, Scottsdale, AZ), 800 µM copper (II ...
-
bioRxiv - Biochemistry 2023Quote: ... 100 μg of lysate were reacted with click reagents to give final concentrations as follows: 100 µM of diazo biotin-azide (Click Chemistry Tools, Scottsdale, AZ), 800 µM copper (II ...
-
bioRxiv - Biochemistry 2023Quote: ... 7.5 µL ‘click chemistry master mix’ was added to each sample to give final concentrations of 100 µM of Cy5-azide (Click Chemistry Tools, Scottsdale, AZ), 800 µM copper (II ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: PTH peptides with C terminal Cys residues purified by HPLC were incubated with 3 molar equivalents of DBCO-maleimide (Click Chemistry Tools #A108-100) and the DBCO-peptide products purified by HPLC ...
-
bioRxiv - Immunology 2024Quote: ... SAv-drug conjugation reactions were next incubated for 1 hour with 100 μL azide agarose slurry with gentle mixing (50 μL packed resin; Click Chemistry Tools/Vector Laboratories), after which the resin was removed using Spin-X columns (Corning) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and 44 µL of a 1:1 mixture of BTTAA (Click Chemistry Tools, 77.4 mg mL-1 PBS) and copper sulfate pentahydrate (7.4 mg mL-1 water) ...
-
bioRxiv - Molecular Biology 2021Quote: ... brucei cells (0.7-0.9 × 106 cells/ml) were incubated with 150 µM 5-ethynyl-2’-deoxyuridine (EdU) (Click Chemistry Tools) for 3 hrs before genomic DNA was isolated ...
-
bioRxiv - Developmental Biology 2020Quote: ... ESC were treated with DOX (different concentrations in the range from 150–600 ng/ml) or 500 µM auxin as indicated or incubate with 10µM EdU (Click Chemistry Tools) for 30 minutes before fixation with 4% PFA in PBS for 10 minutes at room temperature ...
-
bioRxiv - Bioengineering 2021Quote: ... 50 μL of 1 mg/mL antibodies were reacted with 1 μL of 10 mM UV-cleavable azido-NHS ester (30 equivalents to antibodies; Click Chemistry Tools) for 2 h ...
-
bioRxiv - Biochemistry 2022Quote: ... RPA-DBD-A4AZP or RPA-DBD-D4AZP (∼4 mM in 5 ml storage buffer) was mixed with 1.5-fold molar excess DBCO-Cy5 or DBCO-Cy3 (Click Chemistry Tools Inc.) and incubated for 2 hours at 4 °C ...
-
bioRxiv - Microbiology 2023Quote: ... and then resuspended to OD600 of 0.6 in 1 mL PBS + 1 μM AZDye 488 DBCO (Click chemistry tools, cat# 1278-1). Cells were covered with aluminum foil and rocked for 1 hour ...
-
bioRxiv - Biochemistry 2023Quote: 10 μM of RPA4AZP (0.5 mL) was mixed with 2X molar excess of the dibenzocyclooctyne-MB543 (DBCO-MB543; Click Chemistry Tools Inc.) dye and incubated at 4 °C for 1.5 h with gentle rocking in dark ...
-
bioRxiv - Molecular Biology 2021Quote: ... RPAAZP (∼ 4 µM in 5 mL of storage buffer) was mixed with 1.5-fold molar excess DBCO-MB543 (Click Chemistry Tools Inc., AZ) or DBCO-Cy3 ...
-
bioRxiv - Biochemistry 2023Quote: ... 330 μL TCEP (14.0 mg/mL in H2O) and 160 μL of either heavy or light isoDTB tags (4 mg in DMSO, Click Chemistry Tools, 1565). The samples were then treated with 120 μL of the heavy (DMSO treated ...
-
bioRxiv - Biochemistry 2024Quote: ... 165 µL TCEP (14.0 mg/mL in H2O) and 160 µL of either heavy or light isoDTB tags (4 mg in DMSO, Click Chemistry Tools, 1565). The samples were then treated with 120 µL of the heavy (DMSO treated ...
-
bioRxiv - Cancer Biology 2024Quote: ... protein lysates from OSU-CLL cells (1e6 cells/mL) were clicked to TAMRA biotin-azide and incubated with streptavidin agarose resin (Click Chemistry Tools; Scottsdale, AZ) to isolate biotin-alkyne-tagged proteins ...
-
bioRxiv - Cancer Biology 2023Quote: ... Monoclonal antibodies at 1 mg mL-1 in 1X PBS were incubated with DBCO-PEG5-NHS Ester linker (A102P, Click Chemistry Tools, Scottsdale, AZ, USA) at a 4:1 molar ratio of crosslinker to antibody for at least 2 h at room temperature ...