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Citations for Click Chemistry Tools :
1 - 47 of 47 citations for Rat Corticosteroid 11 Beta Dehydrogenase Isozyme 2 HSD11B2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... were dissolved in dry DMF (200 µL) in a plastic screw cap tube and 3-azidopropyl-1-amine (11 μL, 110 μmol, Click Chemistry Tools) followed by DIEA (38 μL ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM THPTA (Click Chemistry Tools), and 100 µM copper sulfate ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM biotin azide (Click Chemistry Tools), 10 mM sodium ascorbate made fresh (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mM THPTA (Click Chemistry Tools 1010-100), and 100 µM copper sulfate (Fisher Scientific AC197722500) ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 μM Cy3 azide (Click Chemistry Tools AZ119-1), and 100 mM ascorbic acid freshly prepared and diluted in Gomori buffer (pH 7.4) ...
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bioRxiv - Genetics 2022Quote: ... 5-ethynyl-2’-deoxyuridine (EdU) (Click Chemistry Tools 1381, Scottsdale, AZ) was added to a final concentration of 10 uM ...
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bioRxiv - Microbiology 2022Quote: ... with 2 μM Alexa Fluor 488 azide (Click Chemistry tools #1275) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... 20 µM 5-Ethynyl-2′-deoxyuridine (EdU; Click Chemistry Tools 1149) was added to the cell culture media for 30 minutes to label NSCs in S-phase ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 μl of 2 mM biotin-alkyne-tag (Click Chemistry Tools, 1266), and 5 μl of 200 mM CuSO4 were added in the specified order to 5 ml of PBS (pH 7.8) ...
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bioRxiv - Biophysics 2023Quote: ... for further conjugation with DBCO-PEG4-NHS (Click Chemistry Tools, A134-2) via succinimidyl ester chemistry ...
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bioRxiv - Microbiology 2022Quote: ... AF 488 picolyl azide and 5-ethynyl-2’-deoxycytidine (EdC) (Click Chemistry Tools, Scottsdale, AZ) were used for click chemistry according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... AlexaFlour 488 picolyl azide and 5-ethynyl-2’-deoxycytidine (EdC) (Click Chemistry Tools, Scottsdale, AZ) were used for click-chemistry according to the manufacturer’s instructions.
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bioRxiv - Biophysics 2024Quote: ... IEDDA reaction was performed by applying 1-2 μM tetrazine dye (Click Chemistry Tools, Scottsdale, AZ) to the culture medium and incubating it with the cells at 37°C for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... In every replicate 1 – 2 mg BTD-labeled lysate was clicked to Az800 (AzDye 800 – Click Chemistry tools) as described above ...
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bioRxiv - Bioengineering 2022Quote: ... and 5-days post-surgery along with 5-Ethynyl-2′-deoxyuridine (EdU, Click Chemistry Tools, 3 mg/kg) every day (n = 4-5/group) ...
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bioRxiv - Bioengineering 2022Quote: ... and Click-&-Go Cell Reaction Buffer Kit (Click Chemistry Tools), and 3 ...
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bioRxiv - Microbiology 2023Quote: ... using the Click-&-Go protein enrichment kit (Click Chemistry Tools) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... brucei cells (0.7-0.9 × 106 cells/ml) were incubated with 150 µM 5-ethynyl-2’-deoxyuridine (EdU) (Click Chemistry Tools) for 3 hrs before genomic DNA was isolated ...
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bioRxiv - Cell Biology 2023Quote: ... cells are incubated overnight in culture medium with 5 μM of 5-ethynyl 2’-deoxyuridine (EdU, #1149, Click Chemistry Tools) diluted from a 10 mM stock in DMSO ...
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bioRxiv - Bioengineering 2020Quote: ... cytokines were reacted with a commercial 2-nitrobenzyl linker displaying both NHS ester and DBCO substituents (1160-10, Click Chemistry Tools) followed by addition of poly(ethylene glycol ...
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bioRxiv - Biochemistry 2019Quote: ... artificial amino acid-containing base was incubated at room temperature with 150 µM 5,5′-dithiobis-2-nitrobenzoic acid for 10 minutes before chilling on ice and adding 300 µM DBCO-Cy3 (Click Chemistry Tools) and incubating at 4°C overnight ...
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bioRxiv - Plant Biology 2023Quote: ... 44.5 μL of labelled proteins were incubated with 2 μM Cy5 Picolyl Azide (Click Chemistry Tools, 50 μM stock in DMSO) or Fluorescein Picolyl Azide (Click Chemistry Tools ...
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bioRxiv - Plant Biology 2024Quote: ... 44.5 μl of labelled proteins were treated with 2 μM Cy5 Picolyl Azide (Click Chemistry Tools, 50 μM stock in DMSO) or Fluorescein Picolyl Azide (Click Chemistry Tools ...
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bioRxiv - Developmental Biology 2021Quote: ... stained using the CalFluor 647 Azide Kit (Click Chemistry Tools, Cat#: 1372), and imaged (see “Fluorescent tissue imaging/fluorescence image analysis”).
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bioRxiv - Immunology 2020Quote: ... A 500 μL labeling reaction containing 0.5 mg of ovalbumin in 1X BBS and 50 μL of 2 mM mTz-PEG4-NHS in DMSO (0.2 mM final; Click Chemistry Tools, 1069), pH 8.5 was rotated at 4 °C overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... The click reaction was performed for 1 hour in click staining solution (100 mM Tris pH 8.5, 1 mM CuSO4, 2 mM AZDye-488 Azide (Click Chemistry Tools, 1275-1), and 100 mM ascorbic acid) ...
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bioRxiv - Bioengineering 2023Quote: 7-(Diethylamino)-4- (hydroxymethyl)coumarin (DEAC-OH) was previously synthesized in-house (Method S3) and conjugated to 2-azidoacetic acid (Click Chemistry tools, 1081), 3-azidopropionic acid (Synthonix ...
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bioRxiv - Physiology 2023Quote: ... strain-promoted inverse electron-demand Diels-Alder cycloaddition (SPIEDAC) was performed by adding 1-2 μM tetrazine dye (Click Chemistry Tools, Scottsdale, AZ) to the culture medium and incubating it with the cells at 37°C for 30 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... Alkyne-conjugated biotin and Protein Reaction Buffer kit were from Click Chemistry Tools.
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bioRxiv - Bioengineering 2020Quote: ... or Click-&-Go™ Plus 647 Imaging Kit (Click Chemistry Tools, Scottsdale, AZ) preceding application of antibody staining solutions ...
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bioRxiv - Molecular Biology 2021Quote: The Click reaction was then performed by incubating cells with 100 µL Click buffer (100 µM copper sulfate, 2 mM THPTA, 10 mM fresh sodium ascorbate, 10 µM Cy5 picolyl azide (Click Chemistry Tools 1171-1)) for 1 hour at 37°C in the dark ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates containing 50 μg of protein were then incubated for 2 h with shaking at 37°C with 100 μM TAMRA azide (Click Chemistry Tools Scottsdale, Az), 800 μM copper(II ...
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bioRxiv - Molecular Biology 2022Quote: ... in situ click reactions were performed by incubating cells with 100 µL Click buffer (100 µM copper sulfate, 2 mM THPTA, 10 mM fresh sodium ascorbate, 10 µM Cy3 picolyl azide (Click Chemistry Tools 1178-1)) for 1 hour at 37°C in the dark ...
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bioRxiv - Cell Biology 2021Quote: ... was conducted using the Click-&-Go Protein Reaction Buffer Kit (Click Chemistry Tools 1262) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... sections were click reacted using a Click&Go EdU 488 imaging kit (Click Chemistry Tools, #1324) according to manufacturer instructions before proceeding with subsequent immunolabeling ...
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bioRxiv - Microbiology 2022Quote: ... 5-EU was labeled in permeabilized cells using the Click & Go Kit (Click Chemistry Tools #1263) with 2 μM Alexa Fluor 488 azide (Click Chemistry tools #1275 ...
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bioRxiv - Biochemistry 2020Quote: ... Enrichment of alkyne-modified proteins was conducted using the Click-&-Go Protein Enrichment Kit (Click Chemistry Tools) and the protocols thereof ...
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bioRxiv - Cell Biology 2020Quote: ... The Click-iT reaction was performed with the Click-&-Go Protein Reaction Buffer Kit (Click Chemistry Tools) and AF488 alkyne (Click Chemistry Tools ...
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bioRxiv - Microbiology 2020Quote: ... to AHA containing proteins was carried out using Click-&-Go™ Protein Reaction Buffer Kit (Click Chemistry Tools) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... EdU was labelled using the 647 azide plus kit (Click-&-Go Plus EdU 647, Cat#1353, Click Chemistry Tools) according to the manufacturers protocol ...
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bioRxiv - Bioengineering 2023Quote: ... Sectioned gels were stained for EdU using a 647 fluorescent EdU kit (Click-&-Go EdU 647, Click Chemistry tools) per the manufacturer’s directions ...
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bioRxiv - Cell Biology 2024Quote: ... incorporated EdU was labeled using the Click-&-Go Plus EdU Cell Proliferation Kit for Imaging (Click Chemistry tools Catalog #1314) and imaged with a Leica THUNDER microscope ...
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bioRxiv - Biochemistry 2022Quote: ... An on bead click chemistry reaction was accomplished using 100 µl Click-&-Go click chemistry reaction buffer kit and 40 µM azide-biotin as per the manufacturer’s instructions (Click Chemistry Tools), and incubated in the dark at room temperature for 30 mins with gentle agitation ...
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bioRxiv - Physiology 2023Quote: ... Enriched proteins were eluted for mass spectrometry analysis according to the Click-&-GoTM Dde Protein Enrichment Kit (Click Chemistry Tools, 1153).
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bioRxiv - Neuroscience 2020Quote: ... the neurons were subjected to the FUNCAT reaction for 2 hours where the newly-synthesized AHA incorporated proteins were tagged with an alkyne-fluorophore Alexa-Fluor 555 through click reaction (C10269, CLICK-iT cell reaction buffer kit, Click Chemistry Tools). After 3 washes with TBS50T (0.1%) ...
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bioRxiv - Cell Biology 2021Quote: ... and labeled with 0.5 µM AF488-Picolyl Azide using the Click-&-Go Cell Reaction Buffer kit according to the manufacturers’ instructions (all reagents from Click Chemistry Tools). Cells were subsequently immunolabeled for TDP-43 as described above.
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bioRxiv - Neuroscience 2020Quote: ... the neurons were subjected to the FUNCAT reaction for 2 hours where the newly-synthesized AHA incorporated proteins were tagged with an alkyne-fluorophore Alexa-Fluor 555 through click reaction (C10269, CLICK-iT cell reaction buffer kit, Click Chemistry Tools). After 3 washes with TBS50t ...