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Citations for Click Chemistry Tools :
1 - 47 of 47 citations for Adenovirus Type 5 Particles CMV GFP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... MCF10A cells were treated with 1 mM 5-ethynyl uridine (5-EU; Click Chemistry Tools 1261) for 1 h to label nascent RNA as in (Bryant et al ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 mM BTTA (Click Chemistry Tools) and 50 nM of Picolyl Azide AF488 (Click Chemistry Tools ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 mM THPTA (Click Chemistry Tools), 20 mM Sodium L-Ascorbate (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Biotin-alkyne (#1266-5) and TAMRA-alkyne (#1255-5) were purchased from Click Chemistry Tools (Scottsdale, AZ). O-GlcNAc Chemoenzymatic labeling using the Click-IT™ kit were carried out as recommended by the manufacturer (ThermoFisher) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Ligands (GFP and mCherry) were modified using trans-Cyclooctene (TCO)- PEG4-NHS Ester (Click Chemistry Tools) to generate GFP-TCO and mCherry-TCO ...
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bioRxiv - Bioengineering 2022Quote: ... and 5-days post-surgery along with 5-Ethynyl-2′-deoxyuridine (EdU, Click Chemistry Tools, 3 mg/kg) every day (n = 4-5/group) ...
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bioRxiv - Cell Biology 2023Quote: ... cells are incubated overnight in culture medium with 5 μM of 5-ethynyl 2’-deoxyuridine (EdU, #1149, Click Chemistry Tools) diluted from a 10 mM stock in DMSO ...
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bioRxiv - Cell Biology 2023Quote: ... 200 mM 5-EU (#1261, Click Chemistry Tools) in DMSO is diluted to 1 mM in culture medium and incubated with cells overnight.
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bioRxiv - Biochemistry 2022Quote: ... 100 uM Biotin-Azide (1167-5, Click Chemistry Tools) and incubated for another 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... 400 µM 5-Ethynyl Uridine (5EU) (Click Chemistry Tools) was added for 1 h as indicated ...
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bioRxiv - Bioengineering 2021Quote: ... 5 mM tris(3-hydroxypropyltriazolylmethyl)amine (THPTA; Click Chemistry Tools), 2 mM copper sulfate ...
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bioRxiv - Neuroscience 2022Quote: ... 5 mM Biotin-PEG4-Alkyne (Click Chemistry Tools, Cat#TA105100) and 50 mM TCEP (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... and 5-ethynyldeoxyuridine (EdU, Sigma-Aldrich or Click Chemistry Tools) was at 10mM in DMSO ...
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bioRxiv - Neuroscience 2022Quote: ... and 5 µM AF 594 picolyl azide (Click Chemistry Tools)) for 30 minutes in the dark ...
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bioRxiv - Molecular Biology 2024Quote: ... and 5-ethynyldeoxyuridine (EdU, Sigma-Aldrich or Click Chemistry Tools) was at 10mM in DMSO ...
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bioRxiv - Genetics 2022Quote: ... 5-ethynyl-2’-deoxyuridine (EdU) (Click Chemistry Tools 1381, Scottsdale, AZ) was added to a final concentration of 10 uM ...
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bioRxiv - Microbiology 2022Quote: ... then incubated with 500 μM 5-EU (Click Chemistry Tools #1261) diluted in complete media for 2 hrs ...
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bioRxiv - Neuroscience 2022Quote: ... 20 µM 5-Ethynyl-2′-deoxyuridine (EdU; Click Chemistry Tools 1149) was added to the cell culture media for 30 minutes to label NSCs in S-phase ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μL of 100 mM BTTAA (Click Chemistry Tools, Scottsdale, AZ) in DMSO ...
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bioRxiv - Biochemistry 2023Quote: ... Click reagents Azide-Fluor 545 (Click Chemistry Tools, Inc. AZ109-5), Copper (II ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 μl of 2 mM biotin-alkyne-tag (Click Chemistry Tools, 1266), and 5 μl of 200 mM CuSO4 were added in the specified order to 5 ml of PBS (pH 7.8) ...
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bioRxiv - Cell Biology 2020Quote: ... and 50 mM MB-Fluor 595 azide (Click Chemistry Tools, cat. 1169-5) for click-chemistry labeling (13 ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 μl DBCO-S-S-biotin (Click Chemistry Tools, 10 mM stock in DMSO) was directly added to the reaction mixture and incubated overnight at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 μl DBCO-S-S-biotin (Click Chemistry Tools, 10 mM stock in DMSO) was directly added to the reaction mixture and incubated overnight at 37°C ...
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bioRxiv - Biophysics 2024Quote: ... Cells were incubated with 5 μM picolyl azide dye (Click Chemistry Tools, Scottsdale, AZ) for 10 minutes at room temperature.
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bioRxiv - Physiology 2023Quote: ... Cells selectively labeled with 5 μM picolyl azide dye (Click Chemistry Tools, Scottsdale, AZ) were incubated for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... 5% CO2 in HibA medium supplemented either with 4mM L-azidohomoalanine (AHA; Click Chemistry Tools) or 4mM L-methionine as control ...
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bioRxiv - Developmental Biology 2022Quote: ... and then media was changed to contain DRB with 5-ethynyluridine (EU) (Click Chemistry Tools, 1261 and Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... AF 488 picolyl azide and 5-ethynyl-2’-deoxycytidine (EdC) (Click Chemistry Tools, Scottsdale, AZ) were used for click chemistry according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: Alkynyl myristic acid (YnMyr, CAS No. 82909-47-5) was obtained from Click Chemistry Tools. Azo biotin-azide (CAS No ...
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bioRxiv - Microbiology 2023Quote: ... AlexaFlour 488 picolyl azide and 5-ethynyl-2’-deoxycytidine (EdC) (Click Chemistry Tools, Scottsdale, AZ) were used for click-chemistry according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... Sections were submerged in a 5 µM Cy3 Picolyl Azide dye (Click Chemistry Tools, Scottsdale, AZ) click staining solution (Hatzenpichler and Orphan ...
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bioRxiv - Microbiology 2022Quote: ... 5-EU was labeled in permeabilized cells using the Click & Go Kit (Click Chemistry Tools #1263) with 2 μM Alexa Fluor 488 azide (Click Chemistry tools #1275 ...
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bioRxiv - Genomics 2023Quote: ... The modified DNA was added to 1µl [1.0mM] Disulfide Biotin DBCO (Click Chemistry Tools A112-5) and incubated at 37°C for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... beads (now containing bound protein) were washed and incubated with 5 μM DBCO-488 (Click Chemistry Tools) for 30 min at RT or overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... and the click reaction was initiation by addition of 2.75µl of click chemistry master mix (0.5µl of 5mM azido-rhodamine or tetramethylrhodamine-5-carbonyl azide [Click Chemistry Tools, Scottsdale ...
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bioRxiv - Cell Biology 2024Quote: ... Click-labeling of nascent proteins was performed with 8.3 µM dibenzocyclooctyne (DBCO)−488 (Click Chemistry Tools Catalog #1278-5) in PBS for 30 minutes at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... brucei cells (0.7-0.9 × 106 cells/ml) were incubated with 150 µM 5-ethynyl-2’-deoxyuridine (EdU) (Click Chemistry Tools) for 3 hrs before genomic DNA was isolated ...
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bioRxiv - Microbiology 2021Quote: ... One mg of cell lysate was resuspended in 445µl 1x SDS buffer with 1x EDTA-free protease inhibitors and incubated for 1.5 hours with 55µl of click reaction master-mix consisting of 10µl of 5mM diazo biotin azide (Click Chemistry Tools), 10µl of 50mM TCEP ...
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bioRxiv - Neuroscience 2020Quote: ... Atxn2Q100/+ and HttQ111/+ mice in the 5.5- and 12-month treatment cohorts were IP injected with 180 mpk 5-ethynyl uridine (Click Chemistry Tools) 5 hours prior to sacrifice via lethal IP injection of at least 250 mpk sodium pentobarbital containing euthanasia solution ...
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bioRxiv - Biochemistry 2022Quote: ... RPA-DBD-A4AZP or RPA-DBD-D4AZP (∼4 mM in 5 ml storage buffer) was mixed with 1.5-fold molar excess DBCO-Cy5 or DBCO-Cy3 (Click Chemistry Tools Inc.) and incubated for 2 hours at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... with an azide or alkyne Alexa Fluor 488/594 (AF 594 Alkyne, 1297-1 or AF 488 Picolyl Azide 1276-5, Click Chemistry Tools, USA) for 1 hour in the dark ...
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bioRxiv - Molecular Biology 2021Quote: ... RPAAZP (∼ 4 µM in 5 mL of storage buffer) was mixed with 1.5-fold molar excess DBCO-MB543 (Click Chemistry Tools Inc., AZ) or DBCO-Cy3 ...
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bioRxiv - Genetics 2024Quote: ... permeabilized with 0.5% TritonX-100 for 15 minutes at room temperature and subjected to click labeling reaction using AFDye 488 Azide (Click Chemistry Tools, #1314). The AFDye 488-labeled cells were analyzed by flow cytometry using the FITC channel.
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bioRxiv - Genomics 2022Quote: 100 µl of 100 µM oligo barcode (IDT) in PBS and 10 µl of 10 mM azide-NHS (Click Chemistry Tools cat# 1251-5) in DMSO were mixed at room temperature for two hours ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Antibodies were functionalized by reacting with a 5-fold molar excess of dibenzocyclooctyne-PEG4-NHS ester (DBCO-PEG4-NHS) (Click Chemistry Tools, Scottsdale, AZ) for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Azide-modified antibody was first purified from the sortase reaction mixture using an Amicon 100kDa MWCO centrifugal filter and then reacted with a 5-fold excess of DBCO-AlexaFluor647 (Click Chemistry Tools, Scottsdale, AZ). Fluorescently labeled antibody was purified using centrifugal filtration ...