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Citations for Click Chemistry Tools :
1 - 44 of 44 citations for 26S Proteasome Non ATPase Regulatory Subunit 10 PSMD10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... 50 μL of 1 mg/mL antibodies were reacted with 1 μL of 10 mM UV-cleavable azido-NHS ester (30 equivalents to antibodies; Click Chemistry Tools) for 2 h ...
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bioRxiv - Bioengineering 2022Quote: ... 50 μL of antibodies were reacted with 10 mM photocleavable azido-NHS ester (Click Chemistry Tools) at 1 ...
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bioRxiv - Genomics 2022Quote: ... 10 µL of 10 mM DBCO-NHS (Click Chemistry Tools cat# A134-10) was then added to the antibodies and allowed to rotate at room temperature for one hour 26 ...
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bioRxiv - Neuroscience 2021Quote: ... 10 μM AFDye 488 Azide (Click Chemistry Tools), and 100 mM ascorbic acid in the dark at room temperature for 30 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... NHS-PEG-TCO ester (A137-10, Click Chemistry Tools), poly(ethylene glycol ...
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bioRxiv - Molecular Biology 2021Quote: The Click reaction was then performed by incubating cells with 100 µL Click buffer (100 µM copper sulfate, 2 mM THPTA, 10 mM fresh sodium ascorbate, 10 µM Cy5 picolyl azide (Click Chemistry Tools 1171-1)) for 1 hour at 37°C in the dark ...
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bioRxiv - Genomics 2022Quote: 100 µl of 100 µM oligo barcode (IDT) in PBS and 10 µl of 10 mM azide-NHS (Click Chemistry Tools cat# 1251-5) in DMSO were mixed at room temperature for two hours ...
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bioRxiv - Molecular Biology 2022Quote: ... in situ click reactions were performed by incubating cells with 100 µL Click buffer (100 µM copper sulfate, 2 mM THPTA, 10 mM fresh sodium ascorbate, 10 µM Cy3 picolyl azide (Click Chemistry Tools 1178-1)) for 1 hour at 37°C in the dark ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1.5 µl of 10 mM Azide-PEG4-Biotin (Click Chemistry Tools), the click reaction was conducted for 60 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Antibodies were conjugated to mTz-PEG4-NHS (Click chemistry tools) at first in 1x BBS ...
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bioRxiv - Bioengineering 2021Quote: ... Cy5.5-NHS ester (purchased from Click Chemistry Tools, for Lys-cy5.5 conjugate 10), or DBCO–Cy5.5 (purchased from Click Chemistry Tools ...
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bioRxiv - Molecular Biology 2023Quote: ... were incubated with 10 mM biotin azide (probe condition; Click Chemistry Tools 1265) or 10 mM desthio-biotin (no probe condition ...
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bioRxiv - Bioengineering 2023Quote: ... in PBS and incubated in 10 μM AZDye 488 DBCO (Click Chemistry Tools) for 30 min at 37 °C ...
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bioRxiv - Bioengineering 2024Quote: ... gels were soaked in 10 µM Cy5-N3 (Click Chemistry Tools; Scottsdale, AZ) for 2 hours at 37 °C to block any unreacted BCN groups ...
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bioRxiv - Bioengineering 2024Quote: ... gels were soaked in 10 µM Cy5-N3 (Click Chemistry Tools; Scottsdale, AZ) for 2 hours at 37 °C to label the SPAAC network and then washed 3 x 1 hr in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 μl DBCO-S-S-biotin (Click Chemistry Tools, 10 mM stock in DMSO) was directly added to the reaction mixture and incubated overnight at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 μl DBCO-S-S-biotin (Click Chemistry Tools, 10 mM stock in DMSO) was directly added to the reaction mixture and incubated overnight at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibody was modified with DBCO-Sulfo-NHS ester (Click Chemistry Tools, #A124) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... SpoIVA was labelled with 10-fold molar excess of trans-cyclooctene-PEG3-Maleimide (Click Chemistry Tools) overnight at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... they were reacted with 10:1 Methyltetrazine-sulfo-N-Hydroxysuccinimide ester (Met-NHS) (Click Chemistry Tools):Peptides for an hour at room temperature in Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Immunology 2020Quote: ... 8 μL of 100 mM TCO-PEG4-NHS in DMSO (10 mM final; Click Chemistry Tools, A137), pH 8.5 was rotated at room temperature for 15 min ...
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bioRxiv - Bioengineering 2022Quote: ... 50 μL antibodies were then modified with UV-cleavable azido-NHS ester (Click Chemistry Tools) at 1:15 molar ratio for 2 h ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies were incubated with DBCO-PEG5-NHS Ester linker (Click Chemistry Tools, cat. no. A102P) at a 4:1 molar ratio linker:antibody for 2 h at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... is combined with DBCO-Peg5-NHS Ester (linker; 10 mM in DMSO; Click Chemistry Tools Cat: 1378531-80-6) in 60 molar excess (50 mg of Rabbit IgG and 4.6 mg of linker) ...
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bioRxiv - Neuroscience 2024Quote: ... were submerged in dimethyl sulfoxide (DMSO) with 10 mM dibenzocyclooctyne-PEG-amine (DBCO-PEG4-amine; Click Chemistry Tools, A103P) overnight at room temperature ...
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bioRxiv - Synthetic Biology 2023Quote: ... and dibenzocyclooctyne-sulfo-N-hydroxysuccinimide ester (DBCO-Sulfo-NHS ester, Click Chemistry Tools, prepared as 10 mM stock in DMSO) at a 2500:1:1000 molar ratio of dye:particle:DBCO ...
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bioRxiv - Bioengineering 2024Quote: ... 40 µL of 10 mM Cy7-NHS Ester (#1115) or AZDye 488-NHS Ester (#1338) (both from Click Chemistry Tools) in DMSO was added to the solution dropwise in dark at 4 °C ...
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bioRxiv - Bioengineering 2020Quote: ... cytokines were reacted with a commercial 2-nitrobenzyl linker displaying both NHS ester and DBCO substituents (1160-10, Click Chemistry Tools) followed by addition of poly(ethylene glycol ...
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bioRxiv - Biochemistry 2019Quote: ... artificial amino acid-containing base was incubated at room temperature with 150 µM 5,5′-dithiobis-2-nitrobenzoic acid for 10 minutes before chilling on ice and adding 300 µM DBCO-Cy3 (Click Chemistry Tools) and incubating at 4°C overnight ...
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bioRxiv - Molecular Biology 2022Quote: Cells were washed 3X with PBS/1%FBS (pH 7.4) followed by treatment with 10 μM CalFluor 647 Azide (Click Chemistry Tools), premixed BTTAA-CuSO4 complex ([BTTAA]:[CuSO4]=2:1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed 3X with PBS/1%FBS (pH 7.4) followed by treatment with 10 μM CalFluor 647 Azide (Click Chemistry Tools), premixed BTTAA-CuSO4 complex ([BTTAA]:[CuSO4]=2:1 ...
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bioRxiv - Molecular Biology 2024Quote: ... The reaction was terminated by quenching with a 10 mM Tris-HCl [pH 8.0] buffer supplemented with 50 µM methyltetrazine-DBCO (Click Chemistry Tools). The methanol-preserved cells conjugated with ClickTags were rehydrated following Supplementary Protocol 3.
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bioRxiv - Molecular Biology 2019Quote: Biotin was attached to metabolically labeled RNAs in a 10–20 μl reaction with 4 mM biotin disulfide azide (Click Chemistry Tools), 5 mM CuSO4 (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: HEK293A cells with or without TEAD overexpression was pretreated with DMSO or TM2 in medium with 10% dialyzed fetal bovine serum (DFBS) for 8 hand labeled by Alkynyl Palmitic acid (1165, Click Chemistry Tools) for another 16 h ...
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bioRxiv - Biochemistry 2023Quote: 10 μM of RPA4AZP (0.5 mL) was mixed with 2X molar excess of the dibenzocyclooctyne-MB543 (DBCO-MB543; Click Chemistry Tools Inc.) dye and incubated at 4 °C for 1.5 h with gentle rocking in dark ...
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bioRxiv - Biochemistry 2023Quote: ... was added to the purified base after concentrating and incubated for 10 min on ice before addition of 300 μM dybenzocyclooctane (DBCO)-Cy3 (Click Chemistry Tools) or LD655 (Lumidyne Technologies) ...
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bioRxiv - Biochemistry 2023Quote: ... the substrate was diluted to 100 μM and incubated for 10 minutes at room temperature with 200 μM SulfoCy5 (for stopped-flow measurements, Click Chemistry Tools) or LD555 (for smFRET measurements) ...
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bioRxiv - Biophysics 2023Quote: ... Azide-PEG4-NHS ester (Cat# AZ103-100) and Sulfo-DBCO NHS ester (Cat# A124-10) were purchased from Click Chemistry Tools (Scottsdale, AZ). Alexa 488 Mouse Anti-ZAP70 (pY319)/Syk (Y352 ...
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bioRxiv - Cell Biology 2022Quote: ... were transfected with pcDNA3.1-SARS CoV-2-Spike-C9 and incubated overnight in DMEM supplemented with 10% (v/v) charcoal-stripped FBS with or without 100 μM of clickable fatty acid analogs (Click Chemistry Tools, Scottsdale, AZ) coupled to albumin ...
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bioRxiv - Synthetic Biology 2023Quote: ... Antibodies were then co-incubated with a 20-fold molar excess of NHS ester: PC-biotin-NHS ester (Click Chemistry Tools), Biotin-PEG2-NHS ester (BroadPharma) ...
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bioRxiv - Immunology 2024Quote: ... Successful attachment of azide groups to antibodies was confirmed by a test conjugation with DBCO-AZDye (Click Chemistry Tools/Vector Laboratories) followed by detection of fluor-conjugated antibodies using UltraComp eBeads (ThermoFisher) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Antibodies were functionalized by reacting with a 5-fold molar excess of dibenzocyclooctyne-PEG4-NHS ester (DBCO-PEG4-NHS) (Click Chemistry Tools, Scottsdale, AZ) for 30 minutes at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... sequences were modified with dibenzocyclooctyne (DBCO) for click chemistry reaction with antibody azides by incubation with dibenzocyclooctyne (DBCO)-sulfo-N-Hydroxysuccinimide (NHS)-ester (Click Chemistry Tools cat. no. A124) at a 1:1000 molar ratio for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Monoclonal antibodies at 1 mg mL-1 in 1X PBS were incubated with DBCO-PEG5-NHS Ester linker (A102P, Click Chemistry Tools, Scottsdale, AZ, USA) at a 4:1 molar ratio of crosslinker to antibody for at least 2 h at room temperature ...