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Citations for Click Chemistry Tools :
1 - 24 of 24 citations for 192 IgG Mouse Monoclonal Cy3 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Site-specific labeling with Cy3 and Cy5 fluorophores was done by diluting Cy3 and Cy5 alkyne dyes (Click Chemistry Tools) to the final concentration of 40 µM each in the extracellular buffer and incubating for 15 minutes in the incubator ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 μM Cy3 azide (Click Chemistry Tools AZ119-1), and 100 mM ascorbic acid freshly prepared and diluted in Gomori buffer (pH 7.4) ...
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bioRxiv - Biochemistry 2023Quote: Cy3 and Cy5 maleimide were purchased from Click Chemistry Tools. S6-Cy5/L9-Cy3 ribosomes were prepared by partial reconstitution of ΔS6-30S and ΔL9-50S subunits with S6-41Cys-Cy5 and L11-11Cys-Cy3 proteins as previously described [13 ...
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bioRxiv - Biophysics 2022Quote: ... Stock solutions were made as follows: Cy3 and Cy5 alkyne dyes (Click Chemistry Tools) 10 mM in DMSO ...
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bioRxiv - Microbiology 2020Quote: ... Sections were submerged in a 5 µM Cy3 Picolyl Azide dye (Click Chemistry Tools, Scottsdale, AZ) click staining solution (Hatzenpichler and Orphan ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were labeled with 20μM EdU (Click Chemistry Tools) for 20 min and EdU was clicked to a mixture of Alexa488 and biotin azides at molar ratio of 1 to 50 ...
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bioRxiv - Cell Biology 2022Quote: ... Pex5 was labeled with AF dye 647 (Click Chemistry Tools) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... artificial amino acid-containing base was incubated at room temperature with 150 µM 5,5′-dithiobis-2-nitrobenzoic acid for 10 minutes before chilling on ice and adding 300 µM DBCO-Cy3 (Click Chemistry Tools) and incubating at 4°C overnight ...
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bioRxiv - Biochemistry 2022Quote: ... RPA-DBD-A4AZP or RPA-DBD-D4AZP (∼4 mM in 5 ml storage buffer) was mixed with 1.5-fold molar excess DBCO-Cy5 or DBCO-Cy3 (Click Chemistry Tools Inc.) and incubated for 2 hours at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... was added to the purified base after concentrating and incubated for 10 min on ice before addition of 300 μM dybenzocyclooctane (DBCO)-Cy3 (Click Chemistry Tools) or LD655 (Lumidyne Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... cultures were labeled with 40 μM of AHA for 30 min (Click Chemistry Tools). At the end of the labeling period ...
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bioRxiv - Physiology 2023Quote: ... Cells selectively labeled with 5 μM picolyl azide dye (Click Chemistry Tools, Scottsdale, AZ) were incubated for 10 minutes at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... All fluorophores used to generate fluorescently labeled proteins were purchased from Click Chemistry Tools. Cy3-labeled ...
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bioRxiv - Molecular Biology 2022Quote: ... in situ click reactions were performed by incubating cells with 100 µL Click buffer (100 µM copper sulfate, 2 mM THPTA, 10 mM fresh sodium ascorbate, 10 µM Cy3 picolyl azide (Click Chemistry Tools 1178-1)) for 1 hour at 37°C in the dark ...
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Chemoproteomics of microbiota metabolites reveals small-molecule agonists for orphan receptor GPRC5AbioRxiv - Biochemistry 2021Quote: ... cell lysates prepared as above were labeled with azido-PEG3-biotin (Click Chemistry Tools, AZ104), incubated with high-capacity neutravidin beads (Thermo Scientific Pierce) ...
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bioRxiv - Microbiology 2022Quote: ... 5-EU was labeled in permeabilized cells using the Click & Go Kit (Click Chemistry Tools #1263) with 2 μM Alexa Fluor 488 azide (Click Chemistry tools #1275 ...
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bioRxiv - Cell Biology 2022Quote: ... In every replicate 1 – 2 mg BTD-labeled lysate was clicked to Az800 (AzDye 800 – Click Chemistry tools) as described above ...
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bioRxiv - Microbiology 2023Quote: ... Bacteria were pulse-labeled for 60 min by supplementing 1 mM L-AHA (Click Chemistry Tools, 1066-100) or L-methionine (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... 600 mM NaCl and then the azido-sialic acids were labeled with 30 μM WS DBCO-biotin (Click Chemistry Tools) at room temperature for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... incorporated EdU was labeled using the Click-&-Go Plus EdU Cell Proliferation Kit for Imaging (Click Chemistry tools Catalog #1314) and imaged with a Leica THUNDER microscope ...
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bioRxiv - Molecular Biology 2019Quote: Biotin was attached to metabolically labeled RNAs in a 10–20 μl reaction with 4 mM biotin disulfide azide (Click Chemistry Tools), 5 mM CuSO4 (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: HEK293A cells with or without TEAD overexpression was pretreated with DMSO or TM2 in medium with 10% dialyzed fetal bovine serum (DFBS) for 8 hand labeled by Alkynyl Palmitic acid (1165, Click Chemistry Tools) for another 16 h ...
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bioRxiv - Microbiology 2022Quote: ... L-AHA-containing proteins were labeled with Cy7 by strain-promoted azide-alkyne click-chemistry employing 1 μM dibenzylcyclooctyne-Cy7 (DBCO-Cy7) (Click Chemistry Tools, USA, Scottsdale). After methanol-chloroform extraction ...
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bioRxiv - Cancer Biology 2023Quote: ... Monoclonal antibodies at 1 mg mL-1 in 1X PBS were incubated with DBCO-PEG5-NHS Ester linker (A102P, Click Chemistry Tools, Scottsdale, AZ, USA) at a 4:1 molar ratio of crosslinker to antibody for at least 2 h at room temperature ...