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Citations for Seikagaku :
1 - 17 of 17 citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The volume of concentrated samples was adjusted to 70 ul and 3 ul of Chondroitinase ABC (1.4 U/ml Stock solution, containing BSA, Seikagaku) was added to each sample ...
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bioRxiv - Biochemistry 2021Quote: ... before resuspending in 6.7 ml per g wet weight in MP2 (20 mM KPi buffer pH 7.4, 1.2 M sorbitol, 3 mg per g wet weight zymolyase from Seikagaku Biobusiness) and incubated at 30°C for 60 min ...
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bioRxiv - Biochemistry 2022Quote: ... yeast cells were resuspended in 6.7 ml per g wet weight MP2 buffer (20 mM KPi buffer pH 7.4, 1.2 M sorbitol, 3 mg per g wet weight zymolyase from Seikagaku Biobusiness) and incubated for 1 h at 30°C ...
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bioRxiv - Biochemistry 2023Quote: ... yeast cells were resuspended in 6.7 ml per g wet weight MP2 buffer (20 mM KPi buffer pH 7.4, 1.2 M sorbitol, 3 mg per g wet weight zymolyase 20T from Seikagaku Biobusiness) and incubated for 1 h at 30°C ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended in 200 μl of ice-cold washing buffer and subsequently cell wall was digested by incubating 100 μl of cells with 0.4 mg/ml of Zymolyase 100T (SEIKAGAKU CORPORATION) at 30 °C for 5-15 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Spheroblasting of cells was done with Zymolyase 100T (Seikagaku). Cells expressing GFP-fused proteins were resuspended in PBS containing 4 % formaldehyde and dropped on polyline coated slides ...
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bioRxiv - Microbiology 2022Quote: ... cells were treated with 50 mg/mL zymolyase 20T (Seikagaku Corporation, Japan) in SB buffer for 30 min at 30 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... the resuspended cells were mixed with 15 mg of Zymolyase 100T (Seikagaku Corp.) that was dissolved in 30 ml 1 M sorbitol ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed cells were treated with 50 µg/mL of Zymolyase 20T (Seikagaku Inc., Tokyo Japan) for 30 min to remove the yeast cell wall ...
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bioRxiv - Cell Biology 2020Quote: ... then yeast cell walls were digested by adding 100 µL Zymolyase (10 mg/mL, Seikagaku) for 1 hour at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Then cells were treated for 30min with 0.5 mg ml-1 Zymolyase 100T (Seikagaku Co., Tokyo) in ZK buffer at room temperature ...
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bioRxiv - Genetics 2019Quote: ... 45 mM EDTA) and resuspended at 5 × 107 cells with 10 mg/ml zymolyase-20T (Seikagaku Biobusiness) and 50 mg/ml lysing enzyme (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated in 98 μl Sorbitol buffer + 2 μl of 2.5 mg/ml Zymolyase 100T (SEIKAGAKU CORPORATION) for 90 min (wild type ...
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bioRxiv - Biochemistry 2020Quote: ... Spheroplasts were prepared as described (Daum et al., 1982) using Zymolyase 100T (Seikagaku; 0.5 mg/g cells wet weight). The spheroplasts were resuspended in ice-cold D-buffer (0.6 M sorbitol ...
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bioRxiv - Cell Biology 2022Quote: ... pH 5.8 the cells were resuspended in the same buffer containing 0.25 mg/ml Zymolyase 20T (Seikagaku Corp., Tokyo) and incubated for 2 h at 30 °C ...
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bioRxiv - Microbiology 2021Quote: ... washed with sorbitol buffer (1M sorbitol, 0.1M NaH2PO4, pH 8.0) and the cell wall was digested with Zymolyase 20T (Seikagaku Biobusiness Corporation) for about 35 min at 37° C ...
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bioRxiv - Genetics 2019Quote: Cells were fixed with 1/10 volume of 37% formaldehyde (Wako) and treated with 10 μg/ml Zymolyase 100T (Seikagaku) for 1.5 h ...