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Citations for Seikagaku :
1 - 18 of 18 citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... After adding 5 to 10 µl Zymolyase 20T (Seikagaku, Tokyo, Japan, 25 mg/ml) and 5 to 10 µl lyzing enzyme (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... The volume of concentrated samples was adjusted to 70 ul and 3 ul of Chondroitinase ABC (1.4 U/ml Stock solution, containing BSA, Seikagaku) was added to each sample ...
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bioRxiv - Biochemistry 2021Quote: ... before resuspending in 6.7 ml per g wet weight in MP2 (20 mM KPi buffer pH 7.4, 1.2 M sorbitol, 3 mg per g wet weight zymolyase from Seikagaku Biobusiness) and incubated at 30°C for 60 min ...
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bioRxiv - Biochemistry 2022Quote: ... yeast cells were resuspended in 6.7 ml per g wet weight MP2 buffer (20 mM KPi buffer pH 7.4, 1.2 M sorbitol, 3 mg per g wet weight zymolyase from Seikagaku Biobusiness) and incubated for 1 h at 30°C ...
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bioRxiv - Biochemistry 2023Quote: ... yeast cells were resuspended in 6.7 ml per g wet weight MP2 buffer (20 mM KPi buffer pH 7.4, 1.2 M sorbitol, 3 mg per g wet weight zymolyase 20T from Seikagaku Biobusiness) and incubated for 1 h at 30°C ...
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bioRxiv - Genetics 2019Quote: ... 45 mM EDTA) and resuspended at 5 × 107 cells with 10 mg/ml zymolyase-20T (Seikagaku Biobusiness) and 50 mg/ml lysing enzyme (Sigma) ...
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bioRxiv - Synthetic Biology 2020Quote: ... we added 20 µl of a 2 mg/ml Zymolyase stock solution (SEIKAGAKU, 20 U/mg) to the cell suspension and incubated it for 1h at 37°C for cell wall digestion ...
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bioRxiv - Neuroscience 2020Quote: ... 0.5 μg of AMAC-conjugated disaccharide samples and standard disaccharides (hyaluronic acid – HA, non-sulfated chondroitin - C0S, C4S, C6S; Seikagaku) were separated on a 30% polyacrylamide gel in Tris Glycine buffer for 30-40 min.
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated in 98 μl Sorbitol buffer + 2 μl of 2.5 mg/ml Zymolyase 100T (SEIKAGAKU CORPORATION) for 90 min (wild type ...
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bioRxiv - Microbiology 2022Quote: ... cells were collected by centrifugation at 5,000 x g for 5 min and treated with two units of Zymolyase (Seikagaku Corporation, Japan) for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mU heparinase III (Seikagaku Corp., Tokyo, Japan), 31.3 mM sodium acetate ...
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bioRxiv - Cell Biology 2022Quote: ... containing 1 mU heparinase I (Seikagaku Corp., Tokyo, Japan), 1 mU heparinase II (Iduron ...
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bioRxiv - Molecular Biology 2023Quote: ... VCAN (1:200, Mouse Anti-Versican Antibody 2B1, Seikagaku), and ELN (1:400 ...
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bioRxiv - Molecular Biology 2023Quote: ... DCN (1:1500, Mouse Anti-Dermatan Sulfate Proteoglycan Antibody 6B6, Seikagaku), VCAN (1:200 ...
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bioRxiv - Cell Biology 2021Quote: ... Then cells were treated for 30min with 0.5 mg ml-1 Zymolyase 100T (Seikagaku Co., Tokyo) in ZK buffer at room temperature ...
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bioRxiv - Genetics 2019Quote: Cells were fixed with 1/10 volume of 37% formaldehyde (Wako) and treated with 10 μg/ml Zymolyase 100T (Seikagaku) for 1.5 h ...
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bioRxiv - Immunology 2021Quote: ... The endotoxin level of HMGN1 (< 1 endotoxin unit per mg) was assessed by Endospecy ES-50M Kit (Seikagaku Corporation, Japan). The protein sequence of HMGN1 was identified by Applied Biosystems Procise 492 HT (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... were suspended in 20 mL ice-cold 50 mM Tris–HCl (pH 7.4) buffer containing 1 mg/mL lysozyme (Seikagaku Corporation, 100940) and 1× protease inhibitor cocktail (Nacalai Tesque ...