Labshake search
Citations for Seikagaku :
1 - 19 of 19 citations for 6H Dibenzo a g quinolizinium 5 8 13 13a tetrahydro 2 9 dihydroxy 3 10 dimethoxy 7 methyl 7S 13aS since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... before resuspending in 6.7 ml per g wet weight in MP2 (20 mM KPi buffer pH 7.4, 1.2 M sorbitol, 3 mg per g wet weight zymolyase from Seikagaku Biobusiness) and incubated at 30°C for 60 min ...
-
bioRxiv - Biochemistry 2022Quote: ... yeast cells were resuspended in 6.7 ml per g wet weight MP2 buffer (20 mM KPi buffer pH 7.4, 1.2 M sorbitol, 3 mg per g wet weight zymolyase from Seikagaku Biobusiness) and incubated for 1 h at 30°C ...
-
bioRxiv - Biochemistry 2023Quote: ... yeast cells were resuspended in 6.7 ml per g wet weight MP2 buffer (20 mM KPi buffer pH 7.4, 1.2 M sorbitol, 3 mg per g wet weight zymolyase 20T from Seikagaku Biobusiness) and incubated for 1 h at 30°C ...
-
bioRxiv - Genomics 2021Quote: ... After adding 5 to 10 µl Zymolyase 20T (Seikagaku, Tokyo, Japan, 25 mg/ml) and 5 to 10 µl lyzing enzyme (Sigma ...
-
bioRxiv - Microbiology 2022Quote: ... cells were collected by centrifugation at 5,000 x g for 5 min and treated with two units of Zymolyase (Seikagaku Corporation, Japan) for 30 min at 37 °C ...
-
bioRxiv - Genetics 2019Quote: ... 45 mM EDTA) and resuspended at 5 × 107 cells with 10 mg/ml zymolyase-20T (Seikagaku Biobusiness) and 50 mg/ml lysing enzyme (Sigma) ...
-
bioRxiv - Neuroscience 2022Quote: ... ChondroitinaseABC (10 U/ml) (Seikagaku Corporation), was first diluted 10-fold and then 1/2000 of it was added to the medium to a final concentration of 0.5 mU/ml in the culture medium (Neurobasal supplemented with B-27).
-
bioRxiv - Biochemistry 2020Quote: ... Spheroplasts were prepared as described (Daum et al., 1982) using Zymolyase 100T (Seikagaku; 0.5 mg/g cells wet weight). The spheroplasts were resuspended in ice-cold D-buffer (0.6 M sorbitol ...
-
bioRxiv - Biochemistry 2020Quote: ... The cell was digested in MP2 buffer (1.2M sorbitol, 20mM KH2PO4 pH 7.4) supplemented with 3mg zymolyase/g wet weight (Seikagaku Corporation) for 1 h at 30°C ...
-
bioRxiv - Cell Biology 2019Quote: ... clone 9 and clone 10 was purified using the Qiagen genomic DNA kit (Genomic-tip 20/G) using 30 OD equivalents of material following the manufacturer’s instructions after zymolyase treatment (Seikagaku). A PCR-free library was generated from 10 µg of gDNA and sequenced at the Beijing Genomics Institute (Hong Kong ...
-
bioRxiv - Genetics 2019Quote: Cells were fixed with 1/10 volume of 37% formaldehyde (Wako) and treated with 10 μg/ml Zymolyase 100T (Seikagaku) for 1.5 h ...
-
bioRxiv - Cell Biology 2020Quote: ... heparitinase I at 10 mU/mL (Seikagaku, 100704), chondroitinase ABC at 100 mU/mL (Sigma Aldrich ...
-
bioRxiv - Biochemistry 2021Quote: ... The volume of concentrated samples was adjusted to 70 ul and 3 ul of Chondroitinase ABC (1.4 U/ml Stock solution, containing BSA, Seikagaku) was added to each sample ...
-
bioRxiv - Genomics 2021Quote: ... 10 mM EDTA (pH 8.0) containing 0.125 mg of Zymolyase 20T (Seikagaku) at 37 °C ...
-
bioRxiv - Synthetic Biology 2020Quote: ... we added 20 µl of a 2 mg/ml Zymolyase stock solution (SEIKAGAKU, 20 U/mg) to the cell suspension and incubated it for 1h at 37°C for cell wall digestion ...
-
bioRxiv - Cell Biology 2020Quote: ... then yeast cell walls were digested by adding 100 µL Zymolyase (10 mg/mL, Seikagaku) for 1 hour at RT ...
-
bioRxiv - Neuroscience 2020Quote: ... 0.5 μg of AMAC-conjugated disaccharide samples and standard disaccharides (hyaluronic acid – HA, non-sulfated chondroitin - C0S, C4S, C6S; Seikagaku) were separated on a 30% polyacrylamide gel in Tris Glycine buffer for 30-40 min.
-
bioRxiv - Cell Biology 2022Quote: ... Cells were incubated in 98 μl Sorbitol buffer + 2 μl of 2.5 mg/ml Zymolyase 100T (SEIKAGAKU CORPORATION) for 90 min (wild type ...
-
bioRxiv - Cell Biology 2021Quote: ... The cells were resuspended in 500 μl SP buffer with 0.1% β-mercaptoethanol and 10 μl of 20 mg/ml Zymoylase-20T (Seikagaku). The cells were incubated with soft rotation for 20 min at room temperature (RT) ...