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Citations for Cell Signaling Technology :
1 - 33 of 33 citations for Water RNase Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... We subsequently treated with RNAse (Cell Signaling) and stained with propidium iodide (PI ...
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bioRxiv - Neuroscience 2019Quote: ... and EDTA-free protease inhibitors (Cell Signaling). The lysate was syringed three times through a 23G needle on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... The Propidium Iodide (PI)/RNase Staining Solution was purchased from Cell Signaling. Cells were exposed to cisplatin or MMC for 24 hours prior to the BrdU pulse ...
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bioRxiv - Cancer Biology 2023Quote: ... They were stained with Propidium Iodine (PI/RNase staining-Cell Signaling Technologies), then sorted using a FACS Celeste machine ...
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bioRxiv - Physiology 2020Quote: ... deionised water and a protease/phosphatase inhibitor cocktail (Cell Signaling Technology (CST), Danvers ...
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bioRxiv - Cancer Biology 2023Quote: ... formaldehyde (16% concentrate stock methanol-free, Cell Signaling Technology, 12606) was directly added to the media to a final concentration of 1% and incubated for 15 min ...
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bioRxiv - Genomics 2022Quote: ... brought to 100 μL with ultrapure water and 5 μL PMSF (200 mM, Cell Signaling) were added and incubated for 10 minutes at rt to block Proteinase K activity ...
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bioRxiv - Systems Biology 2019Quote: ... Antibodies in carrier-free buffer were purchased from Cell Signaling Technologies: EGFR pY1068 (clone 1H12 ...
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bioRxiv - Genomics 2019Quote: ... followed by blocking in 1x Animal-Free Block (Cell Signaling Technologies) for 40 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... then blocked in 1X Animal-Free Blocking Solution (Cell Signaling Technologies, 15019) for 30min ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then blocked with Animal-Free Blocking solution (Cell Signaling Technology) for 1h at room temperature ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were then stained with 100 µg/mL propidium iodide with RNase A (Cell Signaling Technology, catalog # 4087) for 1 hour to visualize DNA content.
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bioRxiv - Cell Biology 2021Quote: ... EDTA-free protease inhibitors) and incubated in 10 µg H3K9me3 antibody (1369, Cell Signaling) overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were fixed in ice-cold 70% ethanol and stained with propidium iodide (50 μg/mL)/RNase (100 μg/mL) and rabbit-anti-phospho-HistoneH3-antibody (#9701, Cell Signaling) and goat-anti-rabbit-Alexa-488-conjugated secondary antibody (#R37116 ...
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bioRxiv - Cancer Biology 2023Quote: ... Digestion was stopped by adding 1× stop buffer (digitonin solution, RNase A, and 50 pg S. cerevisiae spike-in DNA (Cell Signaling Technology) for normalization ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were fixed in ice-cold 70% ethanol for at least 30 minutes and were then labeled with propidium iodide (PI)/RNase staining solution (#4087, Cell Signaling Technology) and further incubated for 15 minutes at 37°C ...
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bioRxiv - Pathology 2022Quote: ... tissues were incubated for 30 minutes in Animal free Blocking solution (Cell Signaling 15019S, 1:5) and then with primary antibodies at 4°C ON ...
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bioRxiv - Molecular Biology 2020Quote: ... incubated with serum free media with primary antibody anti-ACE2 (1:100, Cell signaling, 4355S, USA), anti-CTNNB1 (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... the free-floating coronal sections were incubated with 10% normal goat serum (catalog #5425, Cell Signaling) in Tris-HCl-buffered saline ...
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bioRxiv - Biochemistry 2022Quote: ... The cultures were thawed on ice water and 1 mM phenylmethylsulfonyl fluoride [PMSF] with 0.1x protease inhibitor cocktail (Cell Signaling Technology) were added ...
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bioRxiv - Genetics 2020Quote: ... 500 μl of PI (Propidium Iodide) /RNase solution was added and incubated with the cells for 20 min at room temperature (Cell Signaling Technology, 4087). The cell suspension was then applied onto FACS tubes with cell strainer cap (Corning ...
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bioRxiv - Bioengineering 2020Quote: ... each gel was rinsed briefly in deionized water and then stored for >12 hours in 1X tris-buffered saline solution with Tween® (TBST, prepared from Cell Signaling Technology 9997S 10X stock and MilliQ ultrapure water ...
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bioRxiv - Cancer Biology 2021Quote: ... Coverslips were rinsed three times for 5 min in PBS and briefly rinsed in distilled water prior to mounting in Prolong® Gold Antifade Reagent with DAPI (Cell signaling #8961). All Images were acquired using a Zeiss LSM-780 confocal microscope and processed using ZEISS ZEN 3.2 (blue edition ...
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bioRxiv - Cancer Biology 2020Quote: ... The fixed cells were centrifuged and the pellets obtained were collected in flow cytometry tubes and stained with preformulated PI/RNase solution (Cell Signaling Technology, catalog number: 4087s) for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies were added at 1:250 dilution in 1X Animal-Free Blocking Solution (Cell Signaling Technology, 15019L) to the apical side of each Transwell® for an overnight incubation at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... The gel was then rinsed briefly with deionized water and stored in Tris-buffered saline with Tween 20 (TBST, Cell Signaling Technology, Danvers, MA) overnight to remove excess oil and residual antigen-retrieval buffer.
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bioRxiv - Neuroscience 2023Quote: ... Free floating slices were stained for SA-β-gal per the manufacturer’s protocol (Cell Signaling Technology Danvers, MA, Cat#9860). Each slice was rinsed with 1X PBS then transferred to 1X Fixative Solution for 10-15 minutes followed by rinsing twice with 1X PBS ...
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bioRxiv - Bioengineering 2020Quote: ... The gel was then rinsed briefly in deionized water before equilibrating in tris-buffered saline with Tween® (1X TBST, from Cell Signaling Technology 9997S 10X stock) overnight to remove any residual lysis buffer (exchanging the buffer after 2h) ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins samples were processed in SDS free lysis buffer for the assessment of Ras-GTP level using Active Ras Detection Kit (Cell Signaling) by following manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... Cells were then centrifuged again and removed of supernatant before resuspension in isolation buffer (calcium and magnesium-free 1xPBS, 0.1% (w/v) bovine serum albumin (BSA) (Cell Signaling Technology), 2mM EDTA ...
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bioRxiv - Genomics 2023Quote: ... blocked in TBST buffer supplemented with 5% bovine serum albumin (BSA) or 5% fat-free milk and incubated overnight with primary anti-LEF1 antibody (Cell Signaling, #2230), anti-p16 antibody (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... The incubation was performed using the free-floating method at 4°C (48 h) with rabbit anti-p75NTR(1:500; Cell signaling, MA, USA) with 0.6% Triton X-100 in PBS with 5% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... Oligodendrocyte differentiation was assessed by incubating free-floating sections overnight at room temperature with rabbit PDGFRα (D1E1E) XP® antibodies (Cell Signaling, cat #3174,1:200), mouse anti-CC1 (BD Biosciences ...