Labshake search
Citations for Cell Signaling Technology :
1 - 35 of 35 citations for Water Nuclease Free Dist since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... The samples were incubated with 150 units micrococcal nuclease (Cell Signaling) at 37° C for 10 minutes before quenching with 5 μl 0.5 M EDTA and placed on ice ...
-
bioRxiv - Cancer Biology 2023Quote: ... extracts were incubated with 2 µl Micrococcal Nuclease (Cell Signaling, 10011) and sonicated in a Bioruptor (Diagenode ...
-
bioRxiv - Cell Biology 2020Quote: ... Chromatin was digested using 4μL of micrococcal nuclease (MNase; Cell Signaling Technology, cat #10011) for 25 minutes at 37°C ...
-
bioRxiv - Microbiology 2020Quote: ... and then treated with 0.5 μL of micrococcal nuclease (Cell Signaling Technology, Cat. 10011) for 20 min at 37 °C ...
-
bioRxiv - Cancer Biology 2021Quote: ... resuspended in 400 μL buffer B containing 2 μL Micrococcal Nuclease (#10011, Cell Signaling), and incubated for 20 min at 37°C with frequent mixing ...
-
bioRxiv - Neuroscience 2019Quote: ... and EDTA-free protease inhibitors (Cell Signaling). The lysate was syringed three times through a 23G needle on ice ...
-
bioRxiv - Cell Biology 2024Quote: Cleaveage Under Targets and Release Using Nuclease experiment was performed using a CUT & RUN Assay Kit (Cell Signaling, USA), according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2022Quote: ... Chromatin from crosslinked cells was fractionated by digestion with micrococcal nuclease using the SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling) according to the manufacturer protocol ...
-
bioRxiv - Neuroscience 2022Quote: Input control samples were prepared for each treatment condition for whole cell chromatin using micrococcal nuclease (Cell Signaling Technology #10011) digestion to mononucleosomes ...
-
bioRxiv - Physiology 2020Quote: ... deionised water and a protease/phosphatase inhibitor cocktail (Cell Signaling Technology (CST), Danvers ...
-
bioRxiv - Genomics 2023Quote: CUT&RUN experiments were conducted using the CUT&RUN (Cleavage Under Targets & Release Using Nuclease) assay kit (Cell Signaling Technology, #86652) following the manufacturer’s protocol ...
-
bioRxiv - Genetics 2022Quote: CUT&RUN (Cleavage Under Targets & Release Using Nuclease) libraries were prepared with the established CUT&RUN Assay Kit (Cell Signaling, #86652S). The following antibodies were used ...
-
bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used for cleavage under targets & release using nuclease (CUT&RUN) assays: anti-H3K27ac (8173, Cell Signaling Technology, 1:100) and anti-H3K4me1 (5326 ...
-
bioRxiv - Cancer Biology 2023Quote: ... formaldehyde (16% concentrate stock methanol-free, Cell Signaling Technology, 12606) was directly added to the media to a final concentration of 1% and incubated for 15 min ...
-
bioRxiv - Genomics 2022Quote: ... brought to 100 μL with ultrapure water and 5 μL PMSF (200 mM, Cell Signaling) were added and incubated for 10 minutes at rt to block Proteinase K activity ...
-
bioRxiv - Systems Biology 2019Quote: ... Antibodies in carrier-free buffer were purchased from Cell Signaling Technologies: EGFR pY1068 (clone 1H12 ...
-
bioRxiv - Genomics 2019Quote: ... followed by blocking in 1x Animal-Free Block (Cell Signaling Technologies) for 40 minutes ...
-
bioRxiv - Cell Biology 2020Quote: ... then blocked in 1X Animal-Free Blocking Solution (Cell Signaling Technologies, 15019) for 30min ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cells were then blocked with Animal-Free Blocking solution (Cell Signaling Technology) for 1h at room temperature ...
-
bioRxiv - Cell Biology 2021Quote: ... EDTA-free protease inhibitors) and incubated in 10 µg H3K9me3 antibody (1369, Cell Signaling) overnight at 4°C ...
-
bioRxiv - Pathology 2022Quote: ... tissues were incubated for 30 minutes in Animal free Blocking solution (Cell Signaling 15019S, 1:5) and then with primary antibodies at 4°C ON ...
-
bioRxiv - Molecular Biology 2020Quote: ... incubated with serum free media with primary antibody anti-ACE2 (1:100, Cell signaling, 4355S, USA), anti-CTNNB1 (1:100 ...
-
bioRxiv - Neuroscience 2023Quote: ... the free-floating coronal sections were incubated with 10% normal goat serum (catalog #5425, Cell Signaling) in Tris-HCl-buffered saline ...
-
bioRxiv - Biochemistry 2022Quote: ... The cultures were thawed on ice water and 1 mM phenylmethylsulfonyl fluoride [PMSF] with 0.1x protease inhibitor cocktail (Cell Signaling Technology) were added ...
-
bioRxiv - Bioengineering 2020Quote: ... each gel was rinsed briefly in deionized water and then stored for >12 hours in 1X tris-buffered saline solution with Tween® (TBST, prepared from Cell Signaling Technology 9997S 10X stock and MilliQ ultrapure water ...
-
bioRxiv - Cancer Biology 2021Quote: ... Coverslips were rinsed three times for 5 min in PBS and briefly rinsed in distilled water prior to mounting in Prolong® Gold Antifade Reagent with DAPI (Cell signaling #8961). All Images were acquired using a Zeiss LSM-780 confocal microscope and processed using ZEISS ZEN 3.2 (blue edition ...
-
bioRxiv - Cell Biology 2023Quote: ... Primary antibodies were added at 1:250 dilution in 1X Animal-Free Blocking Solution (Cell Signaling Technology, 15019L) to the apical side of each Transwell® for an overnight incubation at 4°C ...
-
bioRxiv - Bioengineering 2023Quote: ... The gel was then rinsed briefly with deionized water and stored in Tris-buffered saline with Tween 20 (TBST, Cell Signaling Technology, Danvers, MA) overnight to remove excess oil and residual antigen-retrieval buffer.
-
bioRxiv - Neuroscience 2023Quote: ... Free floating slices were stained for SA-β-gal per the manufacturer’s protocol (Cell Signaling Technology Danvers, MA, Cat#9860). Each slice was rinsed with 1X PBS then transferred to 1X Fixative Solution for 10-15 minutes followed by rinsing twice with 1X PBS ...
-
bioRxiv - Bioengineering 2020Quote: ... The gel was then rinsed briefly in deionized water before equilibrating in tris-buffered saline with Tween® (1X TBST, from Cell Signaling Technology 9997S 10X stock) overnight to remove any residual lysis buffer (exchanging the buffer after 2h) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Proteins samples were processed in SDS free lysis buffer for the assessment of Ras-GTP level using Active Ras Detection Kit (Cell Signaling) by following manufacturer’s instructions.
-
bioRxiv - Developmental Biology 2020Quote: ... Cells were then centrifuged again and removed of supernatant before resuspension in isolation buffer (calcium and magnesium-free 1xPBS, 0.1% (w/v) bovine serum albumin (BSA) (Cell Signaling Technology), 2mM EDTA ...
-
bioRxiv - Genomics 2023Quote: ... blocked in TBST buffer supplemented with 5% bovine serum albumin (BSA) or 5% fat-free milk and incubated overnight with primary anti-LEF1 antibody (Cell Signaling, #2230), anti-p16 antibody (ThermoFisher ...
-
bioRxiv - Neuroscience 2024Quote: ... The incubation was performed using the free-floating method at 4°C (48 h) with rabbit anti-p75NTR(1:500; Cell signaling, MA, USA) with 0.6% Triton X-100 in PBS with 5% BSA ...
-
bioRxiv - Neuroscience 2023Quote: ... Oligodendrocyte differentiation was assessed by incubating free-floating sections overnight at room temperature with rabbit PDGFRα (D1E1E) XP® antibodies (Cell Signaling, cat #3174,1:200), mouse anti-CC1 (BD Biosciences ...