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Citations for Cell Signaling Technology :
1 - 44 of 44 citations for Superose 12 Prep Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... ChIP-Grade protein G magnetic beads (Cell Signaling, 9006S), 25 μl per IP ...
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bioRxiv - Developmental Biology 2021Quote: ... and H3K27me antibody (ChIP grade from Cell Signaling, #07-449) has been described previously (Chen et al ...
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bioRxiv - Cancer Biology 2021Quote: ... and 30 μL ChIP-Grade Protein G Magnetic Beads (#9006, Cell Signaling) were added ...
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bioRxiv - Cell Biology 2021Quote: ... and 12 μg PY100 (Cell Signaling Technology) for 8 hours at 4 °C with rotation ...
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bioRxiv - Cell Biology 2021Quote: ... ChIP-Grade protein G magnetic beads (Cell Signaling, 9006S - 25 μL per IP) were pre-blocked in pre-blocking buffer (PBS/EDTA containing 0.5% BSA ...
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bioRxiv - Cell Biology 2024Quote: ... normal rabbit IgG (12-370, Cell Signaling Technology), dextran Oregon Green 488 (D7172 ...
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bioRxiv - Neuroscience 2020Quote: ... 12 or 25 µM LY294002 (Cell Signaling Technology, Inc.) was treated 1 h before the TC-HT.
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bioRxiv - Molecular Biology 2021Quote: ... Supernatants were precleared with 300uL ChIP-grade Protein G magnetic beads (Cell Signaling Cat. 9006) and then incubated rotating overnight at 4C with 10 µg anti-HA ChIP- grade antibody (Abcam ab9110) ...
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bioRxiv - Cell Biology 2021Quote: ... and ChIP grade Protein-G agarose beads were purchased from Cell Signaling Technology (Danvers, MA). Anti-TRPC6 (#PA5-20256) ...
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bioRxiv - Cancer Biology 2022Quote: ... The immunocomplexes were then conjugated with ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology) for 2 hours at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-PU.1 (1 μg per IP prep; 2266S; Cell Signaling Technology). qPCR analysis was conducted on a QuantStudio 6 Flex Real-Time PCR System (4485697 ...
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bioRxiv - Biochemistry 2020Quote: ... then pre-cleared with 20 μL ChIP-grade Protein G Magnetic Beads (Cell Signaling Technology; 9006) for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The ChIP-grade magnetic beads coated with protein G were purchased from Cell Signaling Technology (USA). The antibodies used in immunoprecipitation were Pol II (#39097 ...
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bioRxiv - Cancer Biology 2020Quote: ... The SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina (Cell Signaling, #56795) was used to prepare the DNA library according of manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... HA-tagged proteins were immunoprecipitated with anti-HA and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology). Flag-tagged proteins were immunoprecipitated with Anti-FLAG M2 Magnetic Beads (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were constructed using the DNA Library Prep Kit for Illumina Systems (Cell Signaling Technology, 56795S) and Multiplex-oligos for Illumina Systems (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2022Quote: ... Sequencing libraries were built using DNA Library Prep Kit for Illumina (Cell Signaling Technology, Danvers, MA). Paired-end sequencing was performed on the Illumina NextSeq instrument at the Harvard University Bauer Core ...
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bioRxiv - Cancer Biology 2023Quote: ... and the SimpleChIP ChIP-seq DNA Library Prep Kit for Illumina (Cell Signaling Technology, Cat. #56795) were used with protocol modifications for CUT&RUN as recommended by Cell Signaling ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Badrilla: PLB pSer16 antibody Catalogue No.: A010-12) and total protein (Cell signaling: Troponin I Antibody #4002 and Phospholamban (D9W8M ...
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bioRxiv - Immunology 2020Quote: ... The same amount of protein was immunoprecipitated with anti-NEMO and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology). The IKKα/β kinase activity in the IP complex was tested by using IKKβ Kinase Enzyme Kit (Promega ...
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bioRxiv - Genetics 2021Quote: ... the samples were incubated with 30 ml of ChIP Grade Protein G magnetic beads (Cat# 9006S; Cell Signaling Technology; Danvers, MA) for 2 hours at 4°C under gentle rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... Individual ChIP experiments were performed on pre-cleared chromatin using antibody-coupled ChIP grade Protein G magnetic beads (Cell Signaling Technology). POLR3A antibody was obtained from Abcam (ab96328 lot#GR318563) ...
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bioRxiv - Cancer Biology 2023Quote: ChIP-Seq libraries were prepared using the DNA Library Prep Kit for Illumina (Cell Signaling Technology, Cat 56795) and the Multiplex Oligos for Illumina (Dual Index Primers ...
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bioRxiv - Immunology 2020Quote: ... The cell lysates were incubated overnight at 4 °C with NEMO antibody and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology).
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bioRxiv - Cell Biology 2023Quote: ... The resulting mixtures of protein-DNA and antibody were further incubated with ChIP-Grade Protein G Magnetic Beads (9006, Cell Signaling Technology). After sequential washing with low- and high-salt solutions ...
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bioRxiv - Molecular Biology 2022Quote: ... The remainder of the chromatin solution was incubated with Dynabeads previously coupled with 10 μg ChIP grade antibodies (POGZ, Abcam, ab167408;Flag, Sigma, F1804/F3165; H3K4me3, Abcam, ab1012; H3K27ac, Millipore, MABE647; H3K9me3, Abcam, ab176916; H4K20me3, Cell Signaling Technology, 5737s ...
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bioRxiv - Genetics 2024Quote: ... The clarified nuclear extracts were incubated with IgG antibody applied as negative control or target antibody shown in Table 2 with rotation overnight at 4°C and conjugated with Chromatin immunoprecipitation-grade protein G magnetic beads (Cell Signaling Technology). After extensive washing ...
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bioRxiv - Cancer Biology 2022Quote: ... we prepared a library using the SimpleChIP ChIP-seq DNA Library Prep Kit for Illumina (Cell Signaling Technology, 56795). The library was sequenced using MiSeq (Illumina ...
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bioRxiv - Genetics 2022Quote: CUT&RUN libraries were prepared using the SimpleChIP ChIP-seq DNA Library Prep Kit for Illumina (Cell Signaling, #56795S) with SimpleChIP ChIP-seq Multiplex Oligos for Illumina (Cell Signaling ...
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bioRxiv - Cancer Biology 2023Quote: ... The following primary antibodies were used for ChIP: anti-Pol II (1 μg per IP prep; 14958S; Cell Signaling Technology) and anti-PU.1 (1 μg per IP prep ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were harvested and IP was performed on the sonicated chromatin material using a ChIP grade antibody -anti-SP1 antibody (Cell Signaling Technologies, Clone D4C3) or IgG (negative control) ...
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bioRxiv - Molecular Biology 2024Quote: ... and immunoblotting experiments were carried out on 10 μL of resultant IP preps whereby polyubiquitinated MyHC fragments were probed using a polyclonal rabbit IgG antibody (1:1000; Cell Signaling; cat#: 3933). In addition to these IP experiments ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were then incubated overnight (12 h) at 4 °C with mouse anti–NCS-1 (1:500; Cell Signaling). Samples were subsequently incubated for 2 h with Protein-G–Sepharose (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... The isolated GC samples were fixed in 10mL of DMEM/F-12 supplemented with 1% formaldehyde (Cell Signaling Technology, USA) for 10 minutes at room temperature with rotation ...
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bioRxiv - Cell Biology 2023Quote: ... Histone H3 (Cat #9715S) (1:30,000) and IKKγ Kinase (DA10-12) (Cat# 2695T) (1:1000) were from Cell Signaling Technology; antibodies to IKKβ Kinase (Cat# ab124957 ...
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bioRxiv - Immunology 2024Quote: ... 12-thick cryosections were stained in cocktail of rabbit monoclonal antibody against NFκB p65 (Cell Signaling, #8242; dilution 1:200) and rat monoclonal anti-cytokeratin 8 Troma-I antibody (DSHB ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting DNA fragment samples were generated into libraries using the SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® (Cell Signaling Technology, 56795) and SimpleChIP® ChIP-seq Multiplex Oligos for Illumina® (Dual Index Primers ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining sample was incubated o/n on a rotary shaker at 4 °C with 2 μg of the appropriate antibody (IgG, 12-370 Merck; pSTAT1, 7649 Cell Signaling). The next day 25 μl Dynabeads resuspended in CDB/c were added and incubated for at least 1 h at 4 °C on a rotary shaker ...
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bioRxiv - Cancer Biology 2024Quote: ... Pre-washed beads were incubated with antibodies (Normal Rabbit IgG, Millipore Sigma, Cat. #12-370; Normal mouse IgG, Cell Signaling Technology, Cat ...
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bioRxiv - Biophysics 2022Quote: ... 15 μg of total protein was loaded onto an SDS-PAGE (4-12%) to assess LRP6 and β-catenin protein levels using phospho- and total LRP6 (Cell Signaling), active- and total β-catenin (Cell Signaling ...
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bioRxiv - Bioengineering 2020Quote: ... each gel was rinsed briefly in deionized water and then stored for >12 hours in 1X tris-buffered saline solution with Tween® (TBST, prepared from Cell Signaling Technology 9997S 10X stock and MilliQ ultrapure water ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were separated on a 4–12% SDS-PAGE gel followed by standard immunoblot analysis using anti–CISH (Cell Signaling, Clone D4C10, 1:2000) and Vinculin (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... the tissue was mounted on Superfrost Plus precleaned slides (Fisher; catalog # 12-550-15) followed by cover-slipping with Prolong Gold antifade mounting media (Cell Signaling Technology, catalog # 9071S) and sealed with nail polish.
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bioRxiv - Immunology 2022Quote: ... and liver organ samples taken from 12-month-old mice were lysed in ice-cold RIPA buffer supplemented with Protease & Phosphatase Inhibitor Cocktail (Cell Signaling Technology 5872 S, 50 mg/ml) and Benzonase (Sigma-Aldrich E1014 ...