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Citations for Cell Signaling Technology :
51 - 100 of 1803 citations for 4 Tert Butyldimethylsilyl Oxy Methyl 2 Iodothiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... the membrane was incubated overnight at 4 °C with primary antibodies against phospho-ERK1/2 (1:1,000; Cell Signaling), ERK1/2 (1:400 ...
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bioRxiv - Physiology 2024Quote: Sections were incubated for 2 days at 4°C in primary antisera containing anti-aSyn (Cell Signaling, Danvers, MA) at 1:500 in PBS containing 5% normal donkey serum (NDS ...
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bioRxiv - Immunology 2021Quote: ... Sorted cells were immediately incubated overnight with H3K27me3 (anti-Tri-Methyl-Histone H3 (Lys27) (Cell Signaling Technology #Rabbit mAb #9733) or IgG isotype controls (Guinea Pig anti-Rabbit IgG (H+L ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-Tri-Methyl Lysine motif antibody (14680) and Anti-phospho-Akt-Thr308 antibody (9275) were obtained from Cell Signaling Technology.
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bioRxiv - Molecular Biology 2024Quote: ... anti-Tri-Methyl Lysine motif antibody (14680) and Anti-phospho-Akt-Thr308 antibody (9275) were obtained from Cell Signaling Technology. Ant-Vinculin antibody (sc-25336 ...
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bioRxiv - Cancer Biology 2022Quote: ... their nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, 0.5 ug/ml, 4083S, Cell Signaling Technology, MA, USA) and imaged by using an LSM 880 laser scanning confocal microscope (Carl Zeiss AG ...
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bioRxiv - Neuroscience 2023Quote: ... and were then incubated with 25 μg/mL of DAPI (4’,6-diamidino-2-phenylindole dihydrochloride; Cell Signaling Technology, USA) for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated 2-nights at 4°C with rabbit anti-phospho-CREB (Ser133) (1:200, Cell Signaling, mAb #9198), mouse anti-misfolded SOD1 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Blocking buffer was manually removed and replaced with either anti-Asymmetric Di-Methyl Arginine antibody (1:500, Cell Signaling Technology 13522) or anti-Symmetric Di-Methyl Arginine antibody (1:800 Cell Signaling Technology 13222 ...
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bioRxiv - Molecular Biology 2023Quote: ... Rest of the procedure in GFP trap pulldown was same as described in the glutathione pulldown.mono methyl arginine (MMA) antibody (Cell Signaling Technology) CST ...
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bioRxiv - Cancer Biology 2021Quote: ... and incubated overnight at 4°C with the primary antibodies Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (4370S, Cell Signaling) at 1:2000 ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining sample was incubated o/n on a rotary shaker at 4 °C with 2 μg of the appropriate antibody (IgG, 12-370 Merck; pSTAT1, 7649 Cell Signaling). The next day 25 μl Dynabeads resuspended in CDB/c were added and incubated for at least 1 h at 4 °C on a rotary shaker ...
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bioRxiv - Neuroscience 2021Quote: Pericytes viability was assessed using the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay according to the manufacturer’s procedure (Cell Signaling Technology). Cells were plated at the appropriate concentration in 96-well plates under different experimental conditions ...
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bioRxiv - Cancer Biology 2022Quote: ... Pre-cleared lysates were incubated over night at 4°C with end-over-end rotation with 2 µg of anti-Notch1 (D1E11, Cell Signaling) or anti-Cbl-b (G1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were probed overnight at 4 °C with antibodies against the following epitopes: phospho(Ser217/221)-MEK1/2 (1:1000; 41G9; Cell Signaling), MEK1/2 (1:1000 ...
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bioRxiv - Biochemistry 2024Quote: ... 10 mg of lysate representing one biological replicate was incubated for 2 hours at 4 °C with anti-Kv11.1 antibody (Cell Signaling #12889) covalently coupled to protein A/G magnetic beads (Thermo Fisher) ...
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bioRxiv - Neuroscience 2022Quote: ... containing 5% BSA for one hour at RT and incubated overnight at 4°C with anti-flotillin-2 (C42A3) (1:500, #3436, Cell Signaling), anti-calnexin (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... and corresponding polyvinylidene difluoride (PVDF) membranes were incubated overnight at 4°C with various antibodies: rabbit anti-Bcl-2 (1:500; #3498, Cell Signaling), rabbit anti-Bax (1:500 ...
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bioRxiv - Cancer Biology 2020Quote: ... 2.8mg total protein for each sample was digested with trypsin and purified over C18 columns for enrichment with the Symmetric Di-Methyl Arginine Motif Antibody (Cell Signaling #13563). Enriched peptides were purified over C18 STAGE tips46 ...
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bioRxiv - Immunology 2024Quote: ... while the supernatants were carefully removed and used for the sequential di-methyl-Arginine peptide enrichment (di-me-R symmetric, cat.13563S Cell Signaling Technology). Peptides were eluted from the beads twice at room temperature with 50 µL of 0.15% TFA ...
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bioRxiv - Cancer Biology 2024Quote: ... 25 µL of blocked beads were coupled with 5 µg of Acetyl-Histone H3 (Lys27) (D5E4) XP® Rabbit mAb or Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb (Cell Signaling) or Mono-Methyl-Histone H3 (Lys4 ...
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bioRxiv - Cancer Biology 2020Quote: ... for 1 hour and probed overnight at 4°C with the primary antibodies of interest directed against: rabbit anti-BCL-2 (Cell Signaling, CST4223), rabbit anti-BCL-xL (Cell Signaling ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells (2 x106/sample) were collected by centrifugation at 300 g for 4 min and resuspended in cell lysis buffer (Cell Signaling #9803) supplemented with protease inhibitors (Sigma #5056489001) ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated overnight at 4°C with primary antibody detecting light chain 3 type 1 and 2 (Cell Signaling technology, catalog #3868), SOAT1 (catalog #84476) ...
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bioRxiv - Plant Biology 2024Quote: ... The membranes were incubated overnight in agitation at 4°C with Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (#4370; Cell Signaling Technology) in a dilution 1:1000 with PFBS ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... and incubated with primary antibodies at 1:1000 dilution (overnight at 4°C): rabbit polyclonal anti-phospho (Thr202/Tyr204) Erk1/2 (#9101; Cell Signaling Technology), rabbit polyclonal anti-Erk1/2 (#9102 ...
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bioRxiv - Genetics 2024Quote: ... The clarified nuclear extracts were incubated with IgG antibody applied as negative control or target antibody shown in Table 2 with rotation overnight at 4°C and conjugated with Chromatin immunoprecipitation-grade protein G magnetic beads (Cell Signaling Technology). After extensive washing ...
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bioRxiv - Synthetic Biology 2021Quote: ... Membranes were then incubated overnight at 4°C with rabbit anti-phosphorylated ERK1/2 antibody (Cell Signaling Technology; 9101S; LOT: 30; 1:1,000) in TBST containing 5% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... All cell pellets were then resuspended in 100 µL fresh MG medium and stained with 1.4 µM 4’,6-diamidino-2-phenylindole (DAPI) from Cell Signaling Technology (Cat #4083S) for 15 min in the dark at 30 °C in the microaerobic chamber ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated at room temperature for 2 hours or overnight at 4°C with the following primary antibodies: Sirt3 (1:200, Cell Signaling Technology 5490S), TOMM20 (1:500 ...
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bioRxiv - Immunology 2024Quote: ... and equal volume of 2X STOP Buffer (Buffer 2 + 20 μM EDTA + 4 μM EGTA) was added along with 1 pg of Saccharomyces cerevisiae spike-in DNA (Cell Signaling, cat. 29987). Samples were incubated for 15 mins at 37°C and DNA was isolated and purified using Qiagen MinElute Kit according to manufacturer’s protocol and subjected to library amplification.
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bioRxiv - Developmental Biology 2023Quote: ... and the samples were incubated with the following primary antibodies overnight at 4°C: Rabbit anti-p44/42 MAPK (Erk1/2) (1:1000, 4695, Cell Signaling Technology, MA, USA), rabbit anti-phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Immunology 2024Quote: ... An equal volume of 2× STOP Buffer (Buffer 2 plus 20 µM EDTA plus 4 µM EGTA) and 1 pg of Saccharomyces cerevisiae spike-in DNA (Cell Signaling, cat. no. 29987) were added ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was incubated at 4°C overnight with the following primary antibodies: anti-phospho-MEK1/2 (Cell Signaling Technology, Inc., MA, USA, #9122), anti-MEK1/2 (Santa Cruz biotechnology ...
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bioRxiv - Molecular Biology 2024Quote: ... supernatants were transferred to fresh 2 ml tubes and incubated ON at 4°C with either 5 µl α-H2AK119ub1 (D27C4, Cell Signaling Technology #8240), 15 µl α-H3K27me3 (C36B11 ...
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bioRxiv - Microbiology 2024Quote: ... zonula occludens-2 (ZO-2) (Cell Signaling Technologies), and zonula occludens-3 (ZO-3 ...
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bioRxiv - Microbiology 2024Quote: ... zonula occludens-2 (ZO-2) (Cell Signaling Technologies), zonula occludens-3 (ZO-3 ...
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bioRxiv - Microbiology 2020Quote: ... caspase-4 (4450S; Cell Signaling), caspase-5 (D3G4W ...
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Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: ... ATF-4 (#11815, Cell signaling), LC3B (#NB600-1384 ...
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bioRxiv - Cancer Biology 2023Quote: ... 4% formaldehyde (#12606P, Cell Signaling) was added to fix cells for 15 min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... (MEK1/2) (anti-MEK1/2, Cell Signaling, Leiden, The Netherlands) on the other hand.
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bioRxiv - Neuroscience 2024Quote: ... Mitofusin-2 (MFN-2, 1 μg/ml, Cell Signaling Technology), Optic protein atrophy-1 (OPA1 ...
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bioRxiv - Immunology 2022Quote: ... αvinculin (Cell Signaling 4650T, lot 4), αlaminB1 (Cell Signaling 13435 ...
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bioRxiv - Immunology 2022Quote: ... caspase-4 (#4450; Cell Signaling Technology), caspase-8 (#9746 ...
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bioRxiv - Pathology 2023Quote: ... total Histone 4 (Cell Signaling #2592), a-SMA (Dako M085101 ...
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bioRxiv - Cancer Biology 2022Quote: ... and probed overnight at 4°C with the following primary antibodies diluted in 5% BSA in TBST: pMEK1/2 (Cell Signaling Technology, Danvers, MA, USA, 41G9, 1:1000), pERK1/2 (Cell Signaling Technology ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoblotting was performed with phospho-ERK1/2 (pERK1/2) antibody (Cell signaling), anti-AtMPK3 (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... anti-B cell lymphoma-2 (Bcl-2) (Cell Signaling Technology, USA, #3498S) and anti-CCAAT enhancer binding protein-α (CEBP-α ...
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bioRxiv - Neuroscience 2022Quote: ... ERK1/2 (1:1000 mouse anti-total ERK1/2; Cell Signaling Technology #4696 ...
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bioRxiv - Bioengineering 2024Quote: ... Primary antibodies for Erk1/2 and p-ERK1/2 (Cell Signaling Technology), were used at a 1:1000 dilution ...