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Citations for Cell Signaling Technology :
901 - 950 of 1019 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Sections were then blocked with 5% goat serum/2.5% BSA for at least 1 hour before overnight incubation at 4°C with an antibody cocktail consisting of rabbit anti-vimentin (1:100) (catalog #: 5741; Cell Signaling Technologies; Danvers, MA, USA), mouse anti-dystrophin IgG2b (1:50 ...
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bioRxiv - Microbiology 2024Quote: ... and cell extracts were incubated for 3 h at 4°C with beads pre-coupled overnight with rabbit anti-LC3C (Cell Signaling Technology, 14736, 1:250) antibody ...
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bioRxiv - Cancer Biology 2024Quote: ... and then incubated overnight at 4°C with PANX1 CT-412 (Penuela et al., 2007; Penuela et al., 2009) or HA (Cell Signaling 3724, RRID:AB_1549585, Danvers, MA) primary antibody in a humidity chamber ...
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bioRxiv - Biochemistry 2024Quote: ... The membranes were blocked using 5% non-fat milk in TBS-0.05% tween20 (TBST) and incubated overnight at 4 °C with primary antibodies against P-Rex1 and GAPDH (Cell signaling technology #13168 and #5174 respectively). The membranes were washed three times with TBST and then incubated with secondary antibodies in blocking solution for 2 h at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... and probed overnight at 4°C on a shaker with mouse anti-Ty (Bastin et al., 1996) and rabbit anti-HA (Cell Signaling Technologies, cat. no. 3724S). Membranes were then incubated with goat anti-mouse IgG conjugated to HRP (VWR ...
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bioRxiv - Bioengineering 2024Quote: ... The membrane was incubated overnight at 4°C under rotation with the following primary antibodies diluted in TBST (1:1000): mouse anti-CD44 (Cell Signaling Technology, Boston, MA, USA); rabbit anti-MMP2 (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked with 10% goat serum diluted in PBS for 30 min and incubated with the CD44 antibody (Cell Signaling), cat#3570 ...
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bioRxiv - Immunology 2020Quote: ... 10 μM of p38 inhibitor (SB203580) or MEK1/2 inhibitor (U0126) was used (Cell Signaling Technology, Danvers, MA, USA). Cells were monitored by live cell imaging using the BD Pathway 855 Bioimaging System (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... that were pre-incubated with incubated with 10 ug of anti-STAT3 antibody (anti-Stat3, 12640S, Cell Signaling Technology) at 4°C overnight with gentle rotation ...
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bioRxiv - Developmental Biology 2020Quote: Cell lysates were prepared in 1x SDS Sample Buffer (62.5 mM Tris-HCl pH 6.8, 2%SDS, 42mM DTT, 10% glycerol and 0.01% bromophenol blue; Cell Signaling Technology), sonicated to shear DNA and reduce viscosity and then heat denatured ...
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bioRxiv - Developmental Biology 2022Quote: ... rat TS cells were exposed to a phosphatidylinositol 3-kinase (PI3K) inhibitor (LY294002, 10 μM, 9901, Cell Signaling Technology), chloroquine (50 μM for 24 h ...
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bioRxiv - Genetics 2024Quote: PANC1 and MDA-MB-231 cells were lysed for 10 min in 1X RIPA buffer (Cell Signaling Technology, #9806) supplemented with 1X Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Developmental Biology 2022Quote: Whole testis tissue was homogenized in 1 ml per 100 mg tissue 1:10 RIPA buffer (Cell Signaling Technology Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... 10% sucrose, 0.8 M NaCl, 1 mM EDTA, 0.1% sarkosyl and 1X protease/phosphatase inhibitor cocktail, #5872, Cell signaling) in a 15 ml glass homogenizer with 15 up and down strokes at 70% power by hand on ice ...
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bioRxiv - Neuroscience 2023Quote: ... blots were washed 3X 10 minutes with 1X TBST and probed with Horse Anti-Mouse IgG HRP (Cell Signaling) secondary antibody diluted in block for 1 hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were incubated with 10 ug of anti-Nup98 antibody or an irrelevant isotype control (IgG DA1E, Cell signaling) overnight and subsequently incubated with protein G-beads for 2h ...
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bioRxiv - Immunology 2023Quote: ... IRF9 (6F1, hybridoma supernatants from Platanitis et al. 10, 1:20) and V5 (Cell signaling, Catalog # 13202, 1:2000), IRF1 (Cell signaling ...
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bioRxiv - Genetics 2023Quote: ... Red cells were lysed by resuspending the cells in 10 ml red cell lysis buffer (#46232, Cell Signaling Technology) for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Protein lysates (1 mg) underwent overnight incubation with 10 µg of either PAK1 primary antibody (#2602, Cell Signaling Technology) or Rabbit IgG isotype control (#2729 ...
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Sir2 is required for the quiescence-specific condensed three-dimensional chromatin structure of rDNAbioRxiv - Molecular Biology 2024Quote: ... Pol II ChIPs were conducted with an antibody targeting the Rpb1 subunit (10 µL per reaction, Cell Signaling 2629S), similarly conjugated to 20 µL of protein G magnetic beads ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with a FACS buffer (FACS solution supplemented with 4% fetal calf serum) and incubated with anti-rabbit secondary antibody Alexa Fluor 488™ (#4412, Cell Signaling Technology, Danvers, MA, USA)(1:800 ...
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bioRxiv - Neuroscience 2020Quote: Membranes were then incubated 24-48 hours at 4°C in primary antibodies – PERK (1:500 anti-Rbt; Cell Signaling Technologies [CST], Danvers, MA; cat # 3192S), IRE-1α (1:500 anti-Rbt ...
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bioRxiv - Neuroscience 2021Quote: ... The immunodetection of selected proteins was performed overnight at 4 °C with the following antibodies: p62 (Cell Signaling Technology Inc, Danvers, MA, USA, 5114, 1:1000), LC3b (Abcam ab51520 ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1 h at room temperature and incubated with primary antibodies overnight at 4 °C with anti-Cd9 ([1:3000; System Biosciences, Palo Alto, CA, USA] and anti-Gapdh [1:5000; Cell Signaling, Davers, MA, USA; 14C10]).
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bioRxiv - Microbiology 2022Quote: ... the cells were washed twice with the blocking buffer and incubated at 4 °C with primary antibodies (mouse anti-Strep, Qiagen, Cat #: 34850, 1: 150 dilution) (rabbit anti-PDI, Cell Signaling, Cat #: 3501, 1: 200 dilution) (rabbit anti-Flag ...
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bioRxiv - Microbiology 2022Quote: ... the blot was incubated at 4 °C with primary antibodies (rabbit anti-Flag, Cell Signaling, Cat #: 14793, 1:3,000 dilution) (rabbit anti-Calnexin, Cell Signaling, Cat #: 4691, 1:2,000 dilution) (rabbit anti-COX4 ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg/μl (500 μg protein) samples were prepared and incubated overnight at 4 °C in primary antibody against acetyl-lysine (Cell Signaling; Cat No. 9814 (1:100)) ...
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bioRxiv - Cancer Biology 2024Quote: ... then incubated with primary antibody diluted in 1% FBS/PBS overnight at 4°C (MAP2, Cell Signaling # 4542, 1:500 dilution; NEFH, Cell Signaling # 55453, 1:500 dilution). After 3 washes with PBS ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The membranes were probed overnight at 4 °C with the specified primary antibodies at the dilution of 1:1000 (Cell Signaling Technology: RIPK1 Rabbit mAb#3493)) or 1:4000 (β-actin Rabbit mAb#4970)) ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% Tween® 20 Detergent) for 1h and incubated over-night with primary anti-H3K9Ac antibody at 4°C (C5B11, 9694T, Cell Signaling Technology, Danvers, MA, USA). Membranes were incubated for 1 hour with secondary anti-rabbit HRP-conjugated antibody (NA934 ...
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bioRxiv - Cell Biology 2022Quote: ... adipocytes were treated with assay medium +/-insulin for 10 minutes and harvested in cell lysis buffer (Cell Signaling Technology #9803) with phosphatase inhibitor (Thermo Fisher Scientific 78442) ...
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bioRxiv - Genomics 2021Quote: ... Rabbit anti-H3K27me3 (1:100 for bulk profiling or 1:10 for single-cell experimetns, Cell Signaling Technologies Cat# 9733S), Rabbit anti-H3K9me3 (1:100 ...
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bioRxiv - Cancer Biology 2022Quote: ... Two hundred microgram of chromatin was used for IP with 10 μl of STAT3 (1:50, Cat#12640, Cell Signaling) and 4 μg of IgG (1:250 ...
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... These cells were then subjected to ChIP assays with primary antibody against ESRRA (10 μl/IP, Cell Signaling Technology #13826) or normal rabbit IgG ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with PBS containing 1 mM EDTA and 1% FCS, resuspended in culture medium supplemented with Y27632 (10 μM, Gentaur) and DRAQ7 (1.5 μM, Cell Signaling), sorted based on Lgr5 and Sca-1 expression (and absence of DRAQ7 ...
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bioRxiv - Cell Biology 2023Quote: ... two 10-min washes on gyrotary shaker) then imaged by chemiluminescence with a HRP-conjugated secondary antibody (Cell Signaling 7074).
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bioRxiv - Neuroscience 2022Quote: ... At least 3 independent controls and treated samples were probed with the following primary antibodies (in 5% milk, overnight at 4°C): YAP (1:500, Cell signaling Tec., Cat. No. 14074, RRID: AB_2650491), p YAP (1:2000 ...
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bioRxiv - Bioengineering 2023Quote: ... The membranes were blocked in 5% evaporated milk in Tris-based saline with Tween 20 (0.05% TBST) for 1 hour and followed by incubating with primary antibodies overnight at 4 °C (Anti-HA: Cell Signaling Technology, 3724S; anti-GAPDH: Thermo, MA5-15738). After three rinses with TBST ...
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bioRxiv - Microbiology 2024Quote: ... Slides were blocked with 5% normal goat serum in PBS supplemented with 0.3% v/v Triton X-100 for 1 hr at room temperature and stained overnight at 4 °C with primary antibody (LAMP1 – Cell Signaling Technologies [CST] D2D11; CTSB – BioLegend 948001) diluted in antibody dilution buffer (PBS with 1% w/v bovine serum albumin and 0.3% v/v triton X-100) ...
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bioRxiv - Cancer Biology 2022Quote: ... and probed overnight at 4°C with the following primary antibodies diluted in 5% BSA in TBST: pMEK1/2 (Cell Signaling Technology, Danvers, MA, USA, 41G9, 1:1000), pERK1/2 (Cell Signaling Technology ...
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bioRxiv - Microbiology 2020Quote: ... an amount of digested lysate equivalent to 10 mg of protein was subjected to ubiquitin remnant immunoprecipitation according to the manufacturer’s protocol (Cell Signaling Technologies). For phosphorylation analysis ...
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bioRxiv - Genetics 2021Quote: ... Blots were washed (3 × 10 mins) in TBST before and after incubating with a 1:10,000 anti-mouse secondary antibody (Cell Signaling, 7076S) overnight ...
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bioRxiv - Genetics 2021Quote: ... The blots were washed (3 × 10 mins) in TBST before and after incubating with a 1:10,000 anti-rabbit secondary antibody (Cell Signaling, 7074S). The blots were washed overnight before being incubated with a 1:5000 dilution of anti-HSP70-HRP (need catalog number ...
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bioRxiv - Genetics 2021Quote: ... Blots were washed (3 × 10 mins) in TBST before and after incubating with either a 1:10,000 anti-mouse secondary antibody (Cell Signaling, 7076S) or 1:10,000 anti-rabbit secondary antibody (Cell Signaling ...
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bioRxiv - Neuroscience 2020Quote: ... Chromatin solutions were incubated with 10 ug of anti-HIF1α (Cayman, Rabbit, #10006421) antibody or anti-rabbit IgG (Cell Signaling #2729) overnight at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed 3 × 10 min with TBST and incubated for 1 h with the appropriate secondary antibody (Cell Signaling Technologies) diluted in blocking buffer ...
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bioRxiv - Cancer Biology 2020Quote: Human tissue samples and cell preparations were harvested in Complete RIPA Buffer - 1× radio immunoprecipitation buffer (10× RIPA; 9806, Cell Signaling) and one tablet each of PhoStop and Complete Mini (04906845001 and 04693124001 ...
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bioRxiv - Microbiology 2020Quote: ... an amount of digested lysate equivalent to 10 mg of protein was subjected to ubiquitin remnant immunoprecipitation according to the manufacturer’s protocol (Cell Signaling Technologies). For phosphorylation analysis ...
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bioRxiv - Pathology 2023Quote: ... fluorescence images were acquired on a Zeiss Axioscan Z7 at 20X magnification with a 10% tile overlap and analyzed using the Biology Image Analysis Software (BIAS, Cell Signaling). Keratinocytes were identified with a deep neural network on the basis of pan-cytokeratin ...
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bioRxiv - Cancer Biology 2023Quote: Cells were lysed after RA treatment for 48h with 1X lysis buffer (Cell Lysis Buffer 10 ×, Cell Signaling Technology, Danvers, USA), with protease and phosphatase inhibitors ...