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Citations for Jena Bioscience :
1 - 50 of 50 citations for Zika Virus Vero Cell Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Vero E6 cells were incubated with a 1:500 dilution of an anti-dsRNA J2 antibody (Jena Bioscience) in PBS supplemented with 1% FCS at 4°C overnight with shaking ...
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bioRxiv - Microbiology 2020Quote: ... Genome-labeled AdV was produced by growing the virus in A549 cells in the presence of 2.5 μM EdC (5-ethynyl-2’-deoxycytidine, Jena Biosciences) as described 52 ...
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bioRxiv - Microbiology 2019Quote: ... At least 1000 µg of the cell lysates were incubated with Sepharose beads coupled to 7-methylguanosine (m7GTP, Jena Biosciences). Input cell lysates were collected for western blot analysis while the remaining were incubated overnight with continuous rotation at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates (500 mg of protein) were incubated with 50 μl of 7-methyl-GTP-Agarose slurry (Jena Biosciences, AC-155S) for 16 h at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... The precleared lysate was incubated with 40 µl of agarose (blank) (Jena Bioscience) or γ-aminophenyl-m7GTP (C10-spacer)-agarose (Jena Bioscience ...
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bioRxiv - Cell Biology 2020Quote: ... lysates were pre-cleared using blank agarose (AC-001, Jena Biosciences GmbH, Jena, Germany) for 15 min at +4□C prior to γ-Aminophenyl-m7GTP agarose pulldown ...
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bioRxiv - Microbiology 2023Quote: ... lysate was added to 30 µl m7G-functionalized (or control) agarose resin (Jena Bioscience) and incubated at 4°C for 4 h ...
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bioRxiv - Neuroscience 2022Quote: ... 250-300 μg of protein lysate together with 30 μl of 7-methyl GTP-agarose beads (Jena bioscience #AC-141) were incubated for 90 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentrations were measured using BCA and 300 µg of protein lysate was added to 50 µl of m7GTP beads (Jena Bioscience) for 2h at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... The lysate was cleared by centrifugation and RNA was extracted using RNA purification kit as described by the manufacture (Jena Bioscience, PP-210), followed by DNase I treatment ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were then pulsed with OPP (Jena Bioscience) dissolved in media at a final concentration of 20μM for 1h at 37°C ...
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bioRxiv - Biochemistry 2023Quote: 1 mL of mitochondria suspension (corresponding to 0.5 L HEK293-EBNA1-6E suspension cell culture at 1.5 - 2.0 × 106 cells/mL) were supplemented with 100 uL of 100 mM GMPPNP (Jena Bioscience, NU-899-50) and quickly thawn in a water bath at room temperature ...
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bioRxiv - Immunology 2022Quote: ... cells were treated with O-propargyl-puromycin (OPP, 20μM, Jena Bioscience) for 30’ ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated with 5’ethynyluridine (EU) (0.5 mM) (Jena Bioscience) and Click-iT Alexa Fluor 647 labelling kit (Thermofisher ...
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bioRxiv - Biophysics 2023Quote: Leishmania tarentolae cells of the strain T7-TR (Jena Bioscience, Germany) were transfected with the A2AAR expression plasmids linearized by the SwaI restriction enzyme ...
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bioRxiv - Biophysics 2020Quote: Leishmania tarentolae cells of the strain LEXSY host T7-TR (Jena Bioscience) were transformed with the A2AAR expression plasmids linearized by the SmiI restriction enzyme ...
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bioRxiv - Immunology 2023Quote: ... Cells were pulsed with OPP (20μM, Jena Bioscience, cat: NU-931-5) in complete RPMI supplemented with the cytoplasmic translation inhibitor harringtonine (1μM ...
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bioRxiv - Biochemistry 2020Quote: ... transfected cells were treated with 20 ng/ml recombinant human TNFα (Jena Biosciences) for 30 min ...
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bioRxiv - Cell Biology 2019Quote: ... Transfected cells were selected by addition of 100 µg/mL nourseothricin/clonNAT (Jena Bioscience) and cloned by dispensing dilutions into 96-well plates ...
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bioRxiv - Microbiology 2022Quote: ... Cells were selected in YPD with nourseothricin (100 μg/mL; Jena Bioscience, Jena, Germany) after incubation at 30°C for 3 days ...
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bioRxiv - Cell Biology 2023Quote: ... Inflammasome assembly was induced by administering cells with 5mM ATP (Jena Biosciences cat. NU-1010) or nigericin (Invivogen cat ...
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bioRxiv - Molecular Biology 2024Quote: Cells seeded in 6-well plates were treated with 20 μM OP-puro (Jena Bioscience) at 37°C for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... Cells migrating in microfluidic devices were pulsed with 1 mM 5-ethynyl uridine (EU, Jena Bioscience) in complete DMEM for 4 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were subjected to click-chemistry reaction with 20mM of Cy5-or Biotin-azide (Jena Bioscience) for 30min ...
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bioRxiv - Molecular Biology 2022Quote: Cells were washed with PBS and incubated in methionine-free DMEM with 50 μM HPG (Jena Bioscience) and 100 μg/mL ANS ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were applied onto electron microscopy grids covered with a holey gold layer (UltrAuFoil, Quantifoil, Jena Bioscience). The grids were flash-frozen using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were transferred into CUT&Tag wash buffer (20 mM HEPES [pH 7.5] (Jena Bioscience, #CSS-511), 150 mM NaCl (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... HeLa cells were incubated with pre-warmed fresh media with 400 µM 5-ethynyl uridine (5EU, Jena Biosciences) for 1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were starved of methionine for one hour then 4-Azido-L-homoalanine HCl (L-AHA) (Jena Bioscience) was added to the media (final concentration 40μM ...
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bioRxiv - Plant Biology 2023Quote: ... then 5×106 cells were plated on ESAW agar plates containing 450 μg mL-1 nourseothricin (Jena Bioscience), and incubated in constant light at 18 °C.
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bioRxiv - Cell Biology 2021Quote: ... Cells were pulsed (30 min, unless otherwise stated) with methionine-free DMEM containing the methionine analogue HPG (Jena Bioscience) with concomitant inhibition of cytosolic translation by cycloheximide (50 μg/ml) ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were afterwards subjected to an upscaled click chemistry reaction with biotin-picolyl-azide (Jena Bioscience CLK-1167) for one hour at room temperature and washed twice with 10% ethanol in PBS afterwards ...
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bioRxiv - Systems Biology 2022Quote: ... All cell stocks were regularly checked for absence of mycoplasma with the Mycoplasma Detection Kit (Jena Bioscience; # PP-401).
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bioRxiv - Molecular Biology 2020Quote: Cell lines are regularly checked for the absence of Mycoplasma using a PCR based detection kit (Jena Biosciences PP-401).
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bioRxiv - Cancer Biology 2019Quote: ... Transduced RPE1 cells were selected by culturing in the presence of 400 µg/ml nourseothricin (Jena Bioscience, cat # AB-102L). Anti-TRP14 antibody (1:1,000 ...
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bioRxiv - Cell Biology 2021Quote: Cells were grown on coverslips and transcription levels were measured by pulse labeling with 5’ethynyl uridine (EU, Jena Bioscience) in Ham’s F10 medium supplemented with 10% dialyzed FCS and 20 mM HEPES buffer (both Gibco) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were tested at least once in two months for Mycoplasma contamination using Mycoplasma Detection Kit (Jena Bioscience PP-401L).
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bioRxiv - Biochemistry 2021Quote: ... cells were labelled for 20 minutes with EU (5-ethynyl uridine, Jena Bioscience CLK-N002, final concentration at 1 mM). Harvested cells were firstly labeled with Zombie AquaTM (BioLegend ...
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bioRxiv - Cell Biology 2019Quote: ... nascent RNA was labelled by incubating the cells with 400 μM 5-ethynyluridine (5-EU; Jena Bioscience; CLK-N002-10,), which was then visualized with a click-iT mix consisting of 50mM Tris buffer pH8 ...
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bioRxiv - Cancer Biology 2023Quote: ... The depletion medium was then aspirated and cells were incubated in 13 ml of SILAC RPMI pulse media containing 18.1 mg/l AHA (Jena Bioscience), and either 200 mg/l [13C6 ...
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bioRxiv - Cell Biology 2020Quote: ... In vivo labelling of mitochondrial translation products was performed by pulsing cells with methionine-free DMEM containing the methionine analogue HPG (Jena Bioscience) while cytosolic translation was inhibited with 50 μg/ml cycloheximide ...
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bioRxiv - Molecular Biology 2022Quote: ... Supernatants from the centrifugation of the disrupted cells were incubated with 2 ml of nickel-nitrilotriacetic acid (Ni-NTA) agarose (Jena Bioscience) on ice for 30 min ...
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bioRxiv - Immunology 2020Quote: ... purified naïve C57BL/6 B2 cells were preincubated with a mixture of NIP-OVA and HIV-1 p17/p24/gp120 fusion protein (Jena Biosciences) covalently bound to 1 μm beads (1:1 bead:Bcell ratio ...
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bioRxiv - Cell Biology 2023Quote: On-gel mito-FUNCAT was performed as described previously.72,114 HeLa S3 and MPV17L2 KO #1 cells were cultured in 6-well plates in methionine-free medium with 50 µM HPG (Jena Bioscience) and 100 µg/ml anisomycin (Alomone Labs ...
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bioRxiv - Immunology 2023Quote: ... and first PCR reaction was performed on 1 µl of a 1 in 100 diluted freeze-thawed cell pellet using the SCRIPT HF RT-PCR Kit (Jena Bioscience), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... All mutant cell lines were maintained in a medium supplemented with 450 µg/mL nourseothricin (clonNAT) antibiotic (Jena Biosciences, Jena, Germany).
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bioRxiv - Microbiology 2020Quote: ... The cells were then washed again 3x in 1xPBS and then incubated with 5 µM DBCO-488 (Jena Bioscience, CLK-1278-1) at 37 °C for 30 min or 5 µM Click-IT Alexa Fluor® 488 DIBO alkyne dye (ThermoScientific ...
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bioRxiv - Biochemistry 2023Quote: ... the sequence of corresponding constructs of proLEG were cloned into pLEXSY-sat2.1 vectors and transfected into LEXSY P10 host strain of Leishmania tarentolae cells of the LEXSYcon2.1 expression system (Jena Biosciences, Jena, Germany). The resulting constructs carried N-terminal His6-tags and an N-terminal signal sequence for secretion into the supernatant ...
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bioRxiv - Physiology 2024Quote: ... All cell stocks were regularly checked for absence of mycoplasma with the Mycoplasma Detection Kit (Jena Bioscience, Jena, Thüringen, Germany, #PP-401).
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then washed twice and transferred into scCUT&Tag med buffer (20 mM HEPES [pH 7.5] (Jena Bioscience, #CSS-511), 300 mM NaCl (Sigma Aldrich ...