Labshake search
Citations for Cytoskeleton :
1 - 46 of 46 citations for Neutral Red Dyes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... phenol-red free DMEM and incubated with MemGlow dyes (MemGlow 560, MG02-2 and MemGlow 590, MG03-10; Cytoskeleton) in serum-free ...
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bioRxiv - Bioengineering 2022Quote: ... 1X SiR-actin dye (Cytoskeleton Inc.) and 1X verapamil were added to growth media before addition to macrowells at the start of the timecourse ...
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bioRxiv - Bioengineering 2023Quote: ... Membrane dye (Memglow 488, Cytoskeleton Inc.) was diluted to 100 μM concentration ...
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bioRxiv - Cell Biology 2023Quote: ... Precision Red (Cytoskeleton ADV02-A) was used to quantify protein amount in RIPA lysate ...
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bioRxiv - Cell Biology 2020Quote: ... 500 nM SiR-DNA dye (SC007; Cytoskeleton) was added 30 min before imaging.
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bioRxiv - Microbiology 2021Quote: ... Actin-stain 488 (Red, Cytoskeleton Inc.) and DAPI (Blue ...
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bioRxiv - Cell Biology 2022Quote: Cell tracing dyes including SPY555-Actin (Cytoskeleton CY-SC202), SPY650-Tubulin (Cytoskeleton CY-SC503 ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentration was determined using Precision Red (Cytoskeleton) and 60μg of protein were separated using 4-15% SDS-PAGE gels and transferred onto nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2022Quote: ... Precision Red Advanced Protein Assay (ADV02-A; Cytoskeleton), calcein AM (C1430 ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein concentration was quantified using Precision Red (Cytoskeleton) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The COS-7 cells were labelled with PKmito RED (Nanjing Genvivo Biotech Co., Ltd. PKMR-1, CY-SC052)/PKmito DEEP RED (Cytoskeleton, CY-SC055) 15 minutes in DMEM ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was measured with Precision Red Assay (Cytoskeleton). Laemmli sample buffer (Biorad ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein was quantified using the Precision Red (#ADV02; Cytoskeleton) advanced protein assay and 10-20μg was used ...
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bioRxiv - Neuroscience 2023Quote: ... Tubulin was similarly visualized live with a small molecule dye (Cytoskeleton). Astrocytes were labeled for live assays prior to incorporation into miBrains using an AAV (pAAV-CAG-tdTomato ...
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bioRxiv - Cell Biology 2024Quote: ... For staining with SiR-tubulin dye (Cytoskeleton, Inc., cat. #CY-SC002), culture medium was supplemented with 1 µM of SiR-tubulin ...
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bioRxiv - Cell Biology 2023Quote: ... and total protein concentration was measured using Precision Red (Cytoskeleton). Normalized samples were denatured with SDS loading buffer (250mM Tris [pH 6.8] ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were labeled by adding the dyes MemGlow 488 (Cytoskeleton; 1:200), SPY555-Actin (Cytoskeleton ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were labeled with the dyes SPY555-Actin (Cytoskeleton; 1:1,000) and NucSpot Live 650 (Biotium ...
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bioRxiv - Cell Biology 2020Quote: ... reserving a small amount for protein quantification using Precision Red (Cytoskeleton). Prior to measuring GTPase activity ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein quantification was performed using Precision Red Protein Assay Reagent (Cytoskeleton) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... and labeled with 100 nM SiR-actin dye (Cytoskeleton Inc., Denver, CO, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein concentration was determined using Precision Red Advanced Protein Assay (Cytoskeleton, Inc.). 20ug protein were loaded onto 8-12% SDS-PAGE gels and transferred onto nitrocellulose membranes ...
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bioRxiv - Biochemistry 2023Quote: ... and protein concentrations were estimated using the Precision Red reagent (Cytoskeleton Inc). Protein samples were prepared by mixing 1X LDS (Invitrogen) ...
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bioRxiv - Developmental Biology 2021Quote: ... a highly specific fluorescent dye for F-actin (1:250, Cytoskeleton, Inc., CY-SC001), for 3 hours at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... cells were washed with PBS and treated with SiR-DNA dye (CY-SC007, Cytoskeleton) at 300 nM for 6 hours before starting imaging.
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: ... Actin-F was labelled with Sir-Actin dye (1:5000, Cytoskeleton, Denver, CO, USA) for 4 h prior to infection.
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bioRxiv - Cell Biology 2020Quote: ... RPE cells in growth medium were incubated with far-red SiR lysosome (Cytoskeleton) for 60 min and washed before imaging live cells expressing pHLARE ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined using the Precision Red Advanced Protein Assay Reagent (Cytoskeleton ADV02). Extracted proteins (25-35 μg ...
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bioRxiv - Cell Biology 2019Quote: ... and protein concentration determined using the Precision Red™ Advanced Protein Assay Reagent (Cytoskeleton). Respective samples were treated with 300nM Nocodazole for 15 min before lysis ...
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Gα13 loss promotes tumor progression in the KPC transgenic mouse model of advanced pancreatic cancerbioRxiv - Cancer Biology 2021Quote: ... and the protein concentration was determined using Precision Red solution (Cytoskeleton, Inc., Denver, CO) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... and the protein concentration was determined using Precision Red solution (Cytoskeleton, Inc., Denver, CO). Equal amounts of protein were separated with a 10% SDS-PAGE electrophoresis gel ...
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bioRxiv - Cell Biology 2024Quote: The live-cell staining dyes used are as follow: 100 nM SiR-Tubulin (Cytoskeleton, CY-SC002), 1 μM ER-tracker Red (Beyotime ...
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bioRxiv - Neuroscience 2020Quote: ... and protein concentration estimated in the supernatant using Precision Red Advanced Protein Assay (Cytoskeleton, CA). Samples were prepared at 25 µg protein/well with 2X laemmli loading buffer and 2-mercaptoethanol and run on pre-cast 4-15% gels (Biorad) ...
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bioRxiv - Cell Biology 2019Quote: ... and protein concentrations were measured using the Precision Red Advanced Protein Assay Reagent (# ADV02; Cytoskeleton Inc.) and finally equalized with ice-cold lysis buffer to 1.0 mg/ml ...
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bioRxiv - Immunology 2023Quote: ... Some TFF-concentrated EVs were fluorescently labeled with 200 nM MemGlow 700 nm dye (Cytoskeleton, catalog #MG05-10) for 30 min at room temperature (RT ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration of the cleared lysates was determined by Precision Red Advanced Protein Assay Reagent (Cytoskeleton Inc.). Equal amounts of total cell lysates were loaded to 4–12% Bis-Tris or 3–8% Tris-Acetate gels (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... The laminin density was evaluated by coating the dish with laminin conjugated to a fluorescent dye (green fluorescent HiLyte 488; Cytoskeleton), which was observed under a confocal laser scanning microscope (Zeiss LSM710 ...
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bioRxiv - Immunology 2020Quote: ... After fixation and permeabilization of macrophage monolayers incubation of a working solution of the dye (Acti- stain 555,PHDH1, Cytoskeleton) in PBS at 100nM (stock-14µM in methanol ...
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bioRxiv - Cell Biology 2021Quote: ... actomyosin contractility of MECs stably expressing an inducible ROCK construct was induced overnight using 4-hydroxytamoxifen and the actin cortex of these cells was labelled with SiR-actin dye (Cytoskeleton) according to manufacturer’s instructions prior to laser ablation.
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bioRxiv - Cell Biology 2021Quote: ... Lysates were spun at maximum speed for 10mins before protein level was measured using Precision Red (Cytoskeleton, Inc., #ADV02). Samples were run using precast 4-12% NuPAGE Bis-Tris Acrylamide Gels (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: For co-localization of transfected Dre1-sfGFP truncation constructs and either (i) the MTOC (stained with the dye SiR-Tubulin 647; Cytoskeleton, Inc) or (ii ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein lysates were centrifugated for 15 min at > 15000 rpm at 4°C and supernatant was collected and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). 5-10 μg of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... Whole colonic tissue was homogenized in cell lysis buffer and protein concentration was determined using the Precision Red Protein Assay (Cytoskeleton). Samples were sent to Eve Technologies for analysis using their 32-multiplex Mouse Cytokine/Chemokine Array.
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bioRxiv - Developmental Biology 2023Quote: ... Supernatant was collected upon 15min of centrifugation at >15000 rpm at 4°C and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). Equal amounts of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... The tubulin was then mixed with both biotin-conjugated and HiLyte 647 (far-red) labeled porcine tubulin (Cytoskeleton Inc., Denver, CO) and resuspended in PEM buffer (80 mM PIPES pH 6.9 ...
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bioRxiv - Cell Biology 2019Quote: ... HCLE cells were pre-loaded with either CellMask™ Deep Red Plasma membrane stain as described above or 1 μM of SiR-Actin Spirochrome probe (Cytoskeleton Inc., Denver, CO) for 10 minutes at 37°C and 5% CO2 ...