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Citations for Corning :
1 - 37 of 37 citations for Fish Sperm DNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Larval fish were placed in 25 mm ‘baskets’ (Corning Cell Strainer, ~20-30 fish/basket) and transferred into 30 ml EM that contained 100 μM neomycin (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... fish were initially put into netwell inserts (Corning) in 6 well plates containing EM ...
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bioRxiv - Cell Biology 2020Quote: ... Sperm were seeded in poly-L-lysine coated coverslips (Corning #1.5), air-dried for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... flat bottom 96-well plate (Corning; 1 fish per well in 200 ml E3). Prior to experiments ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Fish in the stressed group were gently placed into 1.8-mL cryogenic tubes (Corning®) with openings at both ends (to allow adequate water circulation ...
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bioRxiv - Neuroscience 2021Quote: ... Fish in the stressed group were gently placed into 1.8-mL cryogenic tubes (Corning®) with openings at both ends (to allow adequate water circulation ...
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bioRxiv - Neuroscience 2023Quote: ... Psilocybin was administered by incubating the fish in the psilocybin solution in 6-well plates (Corning, #3516). Desired concentrations of psilocybin were achieved by adding the stock solution to the E3 medium in which the fish swim ...
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bioRxiv - Molecular Biology 2020Quote: ... on tissue culture plates for Western Blotting or onto 18mm sterile glass coverslips for IF/FISH experiments, both of which were pre-coated with sterile 0.3% gelatin (porcine skin gelatin, sigma) or matrigel (Corning)(diluted 1:100) ...
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bioRxiv - Neuroscience 2023Quote: ... 15 larvae per well of 6-well plates (containing Falcon® 70 µm cell strainer [Corning, USA] and 10 ml fish water). Following caffeine exposure ...
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bioRxiv - Microbiology 2021Quote: ... genomic DNA of each isolates was extracted using AxyPerp Bacterial Genomic DNA Miniprep Kit (Corning, New York, USA) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... DNA extraction from the nuclear free water (Corning Molecular Biology Grade Water ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA was eluted with 28 μL of Molecular Biology Grade Water (Corning, USA) for 1 and 2 mm punches or 36 μL of Molecular Biology Grade Water for 3 ...
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bioRxiv - Microbiology 2020Quote: ... and the purified DNA was eluted in 50 μl of DNase-free water (Corning). The second round of PCR consisted of 2 μl of purified first-round PCR product along with the KAPA HiFi Robust Polymerase enzyme and served to incorporate MiSeq adaptors and index oligonucleotides that allowed for multiplexing of samples.
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bioRxiv - Microbiology 2023Quote: ... which were then purified using the efficient AxyPrep™ DNA Gel Extraction Kit from Corning, NY ...
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bioRxiv - Biochemistry 2023Quote: ... We arrayed 700 ng of plasmid DNA in round-bottom 96-well plates (Corning, 353227) and mixed with 200 µL PEIMax (Polysciences ...
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bioRxiv - Developmental Biology 2021Quote: ... DNA was purified using a 1.2X ratio of Axyprep magnetic beads (Axygen, Corning, NY, Cat #14223151). Library quality and tagmentation were assessed on an Agilent Bioanalyzer before pooling and submitting for 150 bp paired-end sequencing on a Illumina HiSeq 4000 at NovoGene.
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bioRxiv - Microbiology 2021Quote: ... DNA extraction from the nuclear free water (Corning Molecular Biology Grade Water, Corning, Glendale, AZ, USA) was performed to capture any potential microbial DNA contamination associated with the CTAB-based method.
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bioRxiv - Microbiology 2020Quote: ... The DNA pellet of dsDNA S1-Fc was dissolved in PBS buffer (Corning Cat.No.21-040-CM) at 2-2.5 μg/μl ...
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bioRxiv - Cancer Biology 2023Quote: DNA extraction: PDTOs from passages 1-3 were cultured to confluence and incubated with Cell Recovery Solution (Corning) for 30 min at 4° C ...
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bioRxiv - Biochemistry 2020Quote: An amino-modified oligo RE-NH2 (Table S3) was covalently coupled to the DNA-BIND 96-well microplate (Corning) according to the instructions of manufacturer to prepare the oligo-coated microplate ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Phage DNA was precipitated by addition of 1 ml 90% ethanol (Pharmco) and 50 μl 3M sodium acetate (Corning). Following incubation on ice for 5 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... 1.0 μg of single plasmid DNA or 0.5 μg of each plasmid DNA for co-transfection experiments was added to 100 μL of RPMI-1640 with L-Glutamine (Corning) and 5 μL of TransIT-LT1 (Mirus) ...
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bioRxiv - Genomics 2020Quote: For base editor DNA on-target experiments HEK293T cells were seeded into 96-well flat-bottom cell culture plates (Corning), transfected 24 h after seeding with 150 ng of base editor and 50 ng of gRNA expression plasmid and 0.5 µl of Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Bioengineering 2020Quote: ... obtained from the Rutgers University Cell and DNA Repository (ND50028; RUCDR) were maintained on Matrigel-coated tissue-culture treated six-well culture plates (Corning) in mTeSR™1 (Stem Cell Technologies) ...
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bioRxiv - Synthetic Biology 2019Quote: ... All fragments obtained by polymerase chain reaction were gel-purified using a DNA Gel Extraction kit (Axygen, Corning, NY, USA) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... The cell-DNA mix was exposed to one single pulse of 1100mV for 30s and subsequently replated on matrigel-coated plates (Corning) with Essential 8™ media (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Polarization measurements were conducted on a Biotek Cytation 5 (peptide and nucleosome) or Molecular Devices Spectramax (DNA) plate reader with low volume plates (Corning) in either 10µl (peptide ...
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bioRxiv - Genomics 2023Quote: ... was combined at a final concentration of 4ng/μL total DNA with varying concentrations of 35I9 in PBS (21-040-CM, Corning) in 10μL total ...
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bioRxiv - Biochemistry 2023Quote: ... DNA and DNA binding proteins were incubated for 30 minutes at room temperature and then transferred to a 384 well plate (Corning). Fluorescence anisotropy was measured using an excitation wavelength of 500 nm and an emission wavelength of 577 nm on a Tecan Spark plate reader fitted with a 50% dichroic 510 mirror ...
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bioRxiv - Neuroscience 2024Quote: ... in addition to 7.32 μg of lentiviral transfer vector DNA in 500 uL of serum-free Dulbecco’s Modified Eagle Medium with High Glucose (Corning, Corning, NY) were added to CalFectin Reagent diluted in an equal volume of serum-free Dulbecco’s Modified Eagle Medium ...
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bioRxiv - Immunology 2021Quote: ... 98 μl of Qubit DNA dye was aliquoted per well of a 96-well black clear bottom plate (Corning, Cat# 3904). 2 μl of plasma sample was added to each well of the assay plate ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 75 nM of a 3′ biotin-labeled DNA substrate (Table S3) was added to a 96 well half-area black microplate (Corning 3993), followed by addition of 3 µL of 2.2 µM UPF1-HD (165 nM ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 75 nM DNA or 38 nM RNA substrate (Table S3) was added to a 96 well half-area black microplate (Corning 3993), followed by addition of 3 µL of 1.1 µM or 550 nM UPF1-HD (82.5 or 41.25 nM final ...
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bioRxiv - Neuroscience 2024Quote: ... in addition to 7.32 μg of lentiviral transfer vector DNA in 500 uL of serum-free Dulbecco’s Modified Eagle Medium with High Glucose (Corning, Corning, NY) were added to CalFectin Reagent diluted in an equal volume of serum-free Dulbecco’s Modified Eagle Medium ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were started by adding protein to DNA in a Corning® 384-well Low Volume Black Round Bottom Polystyrene NBS Microplate (Corning #4514). Data are collected for 50 cycles each lasting 20 seconds ...
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bioRxiv - Genomics 2023Quote: ... following manufacturer’s protocol. Resulting DNA was cleaned using Axygen AxyPrep MAG PCR Clean-Up Kit (cat. MAG-PCR-CL-50) (Corning, Glendale, AZ). DNA was incubated with 2X volume of magnetic bead solution on a shaker for 5 minutes ...
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bioRxiv - Microbiology 2022Quote: ... transfection mixes were prepared using PEI (4 μl per 1 μg of DNA) with the GFP-HIV (6 μg) and VSV-G (4 μg) constructs in serum-free DMEM medium (Corning #10-013-CM) and added dropwise to HEK293T cells that were seeded onto 10-cm dishes a day prior at a density of 5-6 × 106 cells in 10 ml of fresh DMEM with 10% heat inactivated FBS (Sigma-Aldrich #14009C) ...