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Citations for Beckman :
1 - 41 of 41 citations for pMHC1 150 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... To that was dispensed TMPRSS2 (150 nL) in assay buffer (50 mM Tris pH 8, 150 mM NaCl) using a BioRAPTR (Beckman Coulter) to give total reaction volume of 5 µL ...
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bioRxiv - Microbiology 2020Quote: ... 150 V (DelsaMax PRO Light Scattering Analyzer, Beckman Coulter, United States) in distilled water ...
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bioRxiv - Genomics 2021Quote: ... Size selection for fragments >150 bp was performed with AMPure XP (Beckman Coulter). Libraries were PCR amplified with dual index barcode primers for Illumina sequencing (NEB E7600 ...
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bioRxiv - Physiology 2023Quote: ... NA separation was performed using an Ultrasphere column (Beckman, 150×4.6mm C18 5µM) equipped with two Phenomenex C18 filters in a security guard system ...
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bioRxiv - Microbiology 2023Quote: ... 150 µl of the supernatant was ultra-centrifuged using an Air-Fuge (Beckman) (∼133,000 g ...
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bioRxiv - Microbiology 2022Quote: ... DNA fragments of 150-400 bp were selected using SPRIselect Reagent (Beckman Coulter). The quality of each library was evaluated using a Fragment Analyzer (Advanced Analytical Technologies ...
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bioRxiv - Microbiology 2020Quote: ... using a C18 ultrasphere column of 150*4.6 MM (Beckman Ultrasphere™, Fullerton, USA). The phase used was 80/20 (water-acetonitrile) ...
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bioRxiv - Immunology 2023Quote: ... Prepared DNA libraries were size-selected (150-1000 bp) by Ampure XP (Beckman Coulter). Sequencing was performed using NextSeq500 (Illumina).
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bioRxiv - Molecular Biology 2023Quote: ... Amplified fragments were size selected (150 bp-800 bp) using SPRIselect beads (Beckman Coulter, #B23318). Analysis of library quality was done with Agilent 2100 Bioanalyzer using High Sensitivity DNA Analysis kit (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... 150 mM NaCl) and separated by ultracentrifugation at 35,000 rpm in a SW60 rotor (Beckman) at 4 °C for 2.5 h ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Libraries were size-selected for approximately 150 bp inserts using AMPureXP beads (Beckman Coulter, CA, USA). The libraries were quantified using Bioanalyzer and manually multiplexed for sequencing ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Libraries were size-selected for approximately 150 bp inserts using AMPureXP beads (Beckman Coulter, CA, USA). The 179 libraries were quantified using Bioanalyzer and manually multiplexed for sequencing ...
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bioRxiv - Genomics 2021Quote: ... then size-selected for fragments between 150 and 1000 bp using SPRISelect beads (Beckman Coulter, B23319). Libraries were sent for single-end sequencing on the HiSeq 4000 (50 bp single-end reads) ...
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bioRxiv - Cell Biology 2021Quote: ... and then 100-150 µL was diluted into 10 mL of Isoton II diluent (Beckman Coulter #8546719), and 40,000-50,000 cells were measured per sample ...
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Plastid double-strand RNA transgenes trigger small RNA-based gene silencing of nuclear-encoded genesbioRxiv - Plant Biology 2022Quote: ... We size-selected sRNA libraries for the end product of ~150-nt using the SPRIselect Reagent (Beckman Coulter Life Sciences ...
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bioRxiv - Biochemistry 2021Quote: ... The labeled peptides were further purified by C-18 (5 μm, 4.6 mm × 150 mm, Beckman Coulter Inc.) reverse phase-high performance liquid chromatography ...
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Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: ... Nuclei were collected and resuspended in 150 µL water and combined with 300 μL AMPure XP beads (Beckman). DNA-end repair with T4 DNA Polymerase (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... The library DNA within the 150-to 500-bp range was enriched by AMPure XP beads (Beckman Coulter), quantified by NEBNext Library Quant Kit (NEB) ...
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bioRxiv - Plant Biology 2024Quote: ... The sRNA libraries were size-selected for an end product of 150 nt using the SPRIselect Reagent (Beckman Coulter Life Sciences ...
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bioRxiv - Biochemistry 2022Quote: ... To that was dispensed TMPRSS2 (reconstituted in 50% glycerol at 8.75 μM, 22x) in assay buffer (50 mM Tris pH 8, 150 mM NaCl) using a BioRAPTR (Beckman Coulter) to give a total reaction volume of 50 μL ...
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bioRxiv - Biochemistry 2022Quote: ... To that was dispensed TMPRSS2 (reconstituted in 50% glycerol at 8.75 μM, 50x) in assay buffer (50 mM Tris pH 8, 150 mM NaCl, 0.01% Tween20) using a BioRAPTR (Beckman Coulter) to give a total reaction volume of 5 μL ...
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bioRxiv - Plant Biology 2020Quote: ... Small RNA libraries were size-selected with the end product of ∼150 nt using a polyacrylamide/urea gel or the SPRIselect Reagent (Beckman Coulter Life Sciences ...
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bioRxiv - Cell Biology 2021Quote: ... A total of 400 μg of RNA was sedimented by ultracentrifugation for 150 min at 36,000 rpm (Beckman SW40 rotor) at 4 °C in a 7–47 % sucrose gradient (buffered in 50 mM Tris-acetate ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... and cDNA fragments (150 ∼ 200 bp in length) were then purified using the AMPure XP System (Beckman Coulter, Beverly, USA). Paired-end sequencing of libraries (PE150 ...
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bioRxiv - Genomics 2023Quote: ... Mono- and di-nucleosomal DNA fragments (∼150 to 300 bp) were size-selected with SPRIselect magnetic beads (Beckman-Coulter B23317) using “right size selection” and 0.7× beads:sample volume ratio ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysates were sheared with a 25-gauge 5/8 needle and loaded onto CsCl solution (150% w/v) for ultracentrifugation in NVT 65.2 rotor (Beckman coulter) at 42,000 rpm for 20 hours at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... and then centrifuged for 150 min at 39,000 rpm and 4°C in an Optima L-100XP Ultracentrifuge (Beckman-Coulter) using a SW41Ti rotor ...
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bioRxiv - Cancer Biology 2024Quote: ... Homogenates were adjusted to 42.5% sucrose by adding equal volumes of 85% sucrose in 2X HBS (25 mM HEPES, pH6.5, 150 mM NaCl) in an ultracentrifuge tube (344060, Beckman Coulter). 3.5 mL of 35% and 5% sucrose were overlayed to create a discontinuous sucrose gradient ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 ml of each lysate was mixed with 2 ml of 1% sarkosyl and then layered on 2 ml of 150% (w/v) cesium chloride (in 1X TE, pH 7.5) in 4.9 ml OptiSeal tubes (Beckman Coulter, cat #362185). Samples were centrifuged for 18 h at 42,000 rpm in an NVT 65.2 rotor (Beckman Coulter ...
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bioRxiv - Biophysics 2021Quote: Purified 3C DNA from each sample was sonicated to 200-400 bp with a Soniprep 150 probe sonicator at 4°C and purified with a standard Ampure XP Bead protocol (Beckman Coulter) using a 1/1.5 DNA to bead ratio ...
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bioRxiv - Immunology 2021Quote: ... either 150 μl or a maximum of 1 × 106 viable lymphocytes were analyzed using a CytoFLEX LX Flow Cytometer (Beckman Coulter), and data was analyzed with FlowJo software (FlowJo LCC ...
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bioRxiv - Physiology 2022Quote: ... cDNA fragments of 150∼200 bp were selected by purification of library fragments using AMPure XP system (Beckman Coulter, Beverly,USA). PCR was performed with Phusion High-Fidelity DNA polymerase ...
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bioRxiv - Neuroscience 2024Quote: ... were loaded onto 10–50% continuous sucrose density gradients in polysome buffer (20 mM HEPES pH 7.4, 150 mM KCl, 5 mM MgCl2) (Gradient Master™, BioComp) and centrifuged in an SW40Ti swing-out rotor (Beckman Coulter) at 250,000 g for 2 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: The purified AdgI/AdgH complex sample was diluted to 0.5 mg/ml using the sample buffer containing 25 mM Tris-HCl pH 7.5 and 150 mM NaCl and subjected to the sedimentation velocity measurements using an Optima XL-I analytical ultracentrifuge (Beckman-Coulter). Data was analyzed with the SEDFIT and SEDPHAT programs33,34.
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bioRxiv - Genomics 2024Quote: ... Red blood cells were lysed using a 150 mM NH4Cl lysis buffer and samples were measured immediately after staining without fixation on a CytoFLEX cytometer (Beckman Coulter). Among live singlet events HoxB8-derived neutrophils were identified as CD45.1neg B220neg Siglec Fneg F4/80neg CD11bpos Ly6Gpos events ...
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bioRxiv - Genomics 2021Quote: ... We further purified the materials eluted from the columns by adding 150 ul (1:1) of AMPure beads RNAclean XP (Beckman coulter, A63987) and incubating for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... 150 μL of the recovered sample was mixed with 150 μL of 60% Optiprep and layered with 200 μL of 10% Optiprep and 150 μL of the reconstitution buffer in a 0.8 mL tube (Beckman coulter Cat. #344090). The tube was centrifuged at 40,000rpm for 90 minutes in a SW-55 rotor ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA fragments of 150-200bp length were purified to a concentration of 1.5ng/μl using AMPure XP beads (Beckman Coulter, Beverly, USA). Assessment of library effective concentration and RNA quality were performed using Agilent 2100 Bioanalyzer and Qubit2.0 (Thermo Scientific ...
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bioRxiv - Biophysics 2020Quote: ... 150 μl of the sample was ultracentrifuged at 22°C and 205000 × g for 19 min (TLA 100.3, Beckman Coulter Inc., Fullerton, California). The experiments were performed in HEPES buffer ...
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bioRxiv - Neuroscience 2020Quote: ... to obtain fragments in the size range of 150 bp followed by purification with AMPure XP beads (×1.8v/v) (Beckman Coulter A63881, Indianapolis, IN). Purified DNA was resuspended in 45 μl elution buffer ...
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bioRxiv - Microbiology 2023Quote: DNA cleanup was carried out after each step to remove DNA < 150 bp using a 1:1 ratio of AMPure® paramagnetic beads (Beckman Coulter, Inc., USA). Short read paired-end data was assembled using the de novo assembly algorithm ...