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Citations for Beckman :
1 - 46 of 46 citations for Recombinant Rabbit IL21 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... recombinant protein and CD14 antibody (Beckman) were used at 20 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... recombinant protein and anti-CD14 antibody (Beckman) were used at 20 μg/ml ...
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bioRxiv - Immunology 2022Quote: Dynamic light scattering (DLS) experiments of recombinant GBPs and LPS was performed with Delsa Max Pro instrument (Beckman Coulter). GBPs were diluted in buffer C or buffer C (apo ...
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bioRxiv - Molecular Biology 2023Quote: Proteins were collected by centrifugation (Beckman-Coulter JA-14 rotor ...
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bioRxiv - Immunology 2019Quote: ... CECs were pre-incubated with human recombinant CCL5 (1-1000 ng/ml, R&D) and/or CD35 blocking antibody (5-20 μg/ml, clone J3D3, Beckman Coulter) for an hour prior to infection.
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bioRxiv - Cell Biology 2020Quote: ... Protein aggregates were pelleted by ultracentrifugation of the proteins individually at 80,000 rpm in TLA 100 (Beckman Coulter) for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... secondary anti-rabbit-HRP (Beckman Coulter, Ref. 711-035 -152), secondary anti-rat-HRP (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were sedimented in the SW40Ti rotor (Beckman Coulter) at 100,000 × g for 17 h at 4 °C ...
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bioRxiv - Microbiology 2022Quote: Supernatant containing proteins and membrane were ultracentrifuged at 200,000xg (Beckman Coulter Ultracentrifuge ...
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bioRxiv - Biochemistry 2020Quote: ... proteins and liposomes were mixed in polycarbonate tubes (Beckman Coulter) and incubated for 30 min at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein complexes were then fractionated by centrifugation in an SW 41Ti rotor (Beckman) at 38,000 rpm ...
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bioRxiv - Cell Biology 2019Quote: ... The protein solution was then centrifuged at 100,000 rpm in an Airfuge (Beckman Coulter) to remove aggregates ...
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bioRxiv - Cell Biology 2019Quote: ... Intracellular proteins were then stained with anti-Gag KC57 RD1 antibody (1/100, Beckman Coulter) and/or anti-α-tubulin (5 μg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... aggregated proteins were removed by centrifugation at 30,000×g for 20 min (Beckman Coulter, USA). Protein was concentrated by centrifugal spin filters (Millipore ...
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bioRxiv - Bioengineering 2023Quote: ... green fluorescent protein (GFP)-positive cells were FACS-sorted (MoFlo Astrios, Beckman Coulter, California, USA) as single cells into a 96-well plate and clonally propagated ...
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bioRxiv - Genomics 2020Quote: The level of MPL protein on the platelet membrane was measured by flow cytometry (Beckman Coulter FC500) using the monoclonal antibodies ...
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bioRxiv - Biochemistry 2019Quote: ... the soluble Tau protein was centrifuged at 60,000 rpm for 1 hour (Optima Max XP Beckman Coulter) to remove aggregates present if any and 20 μM of soluble Tau protein was incubated in 20 mM BES buffer pH 7.4 with 5 μM of Heparin 17,500 Da in presence of 25 mM NaCl ...
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bioRxiv - Immunology 2020Quote: CCMV-SS and CCMV-Ctt830-SS VLPs were separated from cellular proteins by ultracentrifugation (SW28 rotor, Beckman, Palo Alto ...
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bioRxiv - Microbiology 2023Quote: ... The surface expression level of S proteins (Figure 4B) was measured using CytoFLEX Flow Cytometer (Beckman Coulter) and the data were analyzed using FlowJo software v10.7.1 (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: Antibodies and clones against the following proteins were used (clone, fluorophore, company): CD3 (UCTH1, PECy5, Beckman Coulter), CD7 (Horizon V450 or BUV395 ...
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bioRxiv - Biochemistry 2020Quote: ... The dialysed proteins were clarified by centrifugation in an Optima MAX-XP tabletop ultracentrifuge (Beckman Coulter Life Sciences) (90,720 x g ...
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bioRxiv - Cancer Biology 2022Quote: ... proteins enriched by IgG-agarose beads were eluted and processed with Agencourt AMPure-XP magnetic beads (Beckman Coulter) as previously described [40] ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound protein was separated from liposomes by transferring 100μl of this mixture to 7×20mm PC ultracentrifuge tubes (Beckman) and carefully layering an equal volume of 0.75M sucrose buffer followed by 25μl of sucrose-free HK buffer ...
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bioRxiv - Cancer Biology 2020Quote: Cells expressing different fluorescent proteins were harvested from culture and counted using a ViCELL-XR (Beckman Coulter, Indianapolis, IN). Cells were washed 3 times in PBS to remove remaining cytokine ...
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bioRxiv - Cell Biology 2019Quote: ... 50 μg of each standard protein were loaded on top of the gradient and centrifuged at 29.000 rpm in a SW41 rotor (Beckman) for 29 h at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... The gradients were loaded with 50 μg of recombinant CLASP2 C-term or protein standards of known sedimentation coefficient and centrifuged for 29 h at 29.000 rpm in a SW41 rotor (Beckman). Fractions of 700 μl were collected starting from the bottom of the tube using a peristaltic pump ...
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bioRxiv - Systems Biology 2021Quote: ... Cells expressing the DsRed fluorescent protein were FAC sorted with a MoFlo cell sorter (Beckman Coulter GmbH, Krefeld, Germany) to obtain a highly enriched fraction for neuronal cells.
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bioRxiv - Microbiology 2023Quote: ... The denatured protein fraction was then removed via ultracentrifugation at 100,000 g for 20 min at 4°C (Beckman Coulter Optima XE-90 – IVD ultracentrifuge with a 42.2 Ti rotor) ...
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bioRxiv - Neuroscience 2022Quote: ... The amount of p24 capsid protein was determined by the HIV-1 p24 ELISA antigen assay (Beckman Coulter, Fullerton, CA). Virus from different productions averaged 175 ng/µl of p24 antigen.
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bioRxiv - Biochemistry 2020Quote: ... Protein expression was continued for 24 h before harvesting cells by centrifugation (4°C, 8000 rpm, 10 min in Beckman Coulter Avanti JXN-26 centrifuge ...
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bioRxiv - Biochemistry 2020Quote: ... Protein expression was continued for 18 h before harvesting cells by centrifugation (4°C, 8000 rpm, 10 min in Beckman Coulter Avanti JXN-26 centrifuge ...
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bioRxiv - Biochemistry 2021Quote: ... 300 μL of metabolite pool was aliquoted per target protein replicate and sealed with aluminum foil seals (Beckman Coulter 538619) to create the metabolite chambers ...
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bioRxiv - Biochemistry 2020Quote: ... Protein-liposome complexes were separated from unbound protein by centrifugation (2 h at 45.000 rpm, 4 °C, MLS50 rotor -Beckman Coulter) in the density gradient ...
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bioRxiv - Microbiology 2023Quote: ... Not solubilised protein was removed by a second pelleting at 100×kg for 1 h in Ti70 rotor (Beckman Coulter). The solubilized and denatured protein was loaded on a 5 ml HisTrap HP column (Cytiva) ...
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bioRxiv - Molecular Biology 2023Quote: ... the extract and the protein ladder were overlaid onto separate gradients and centrifuged with an SW41Ti rotor (Beckman Coulter, 20U113807) in an Optima XPN-100 Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... and the uptake of endocytosed fluorescent proteins was analyzed in CD42b-positive population using a CytoFlex S flow Cytometer (Beckman).
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bioRxiv - Biochemistry 2022Quote: ... 750 µL of the 80% Histodenz solution were added and thoroughly mixed with 750µL of the protein-LUV sample in Thinwall centrifuge tubes (Beckman 50.2ti rotor). 1.5 mL of the 30% Histodenz solution was added as the intermediate layer ...
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bioRxiv - Biochemistry 2020Quote: ... Protein samples were clarified by centrifugation using a TLA-55 rotor in an Optima MAX-XP table top ultracentrifuge (Beckman Coulter) (90,720 x g ...
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bioRxiv - Immunology 2022Quote: ... and the expression of the Gag- p24 viral protein was detected with a PE-anti-p24 antibody (clone KC57 RD1; Beckman Coulter). Cell viability was determined using an aqua viability dye for flow cytometry (LIVE/DEAD® Fixable Aqua Dead Cell Stain kit ...
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bioRxiv - Genomics 2023Quote: ... cellular/protein debris were then collected by centrifugation at 1,000xg for 15 minutes at 4°C (Beckman Coulter Allegra X-14R), and the supernatant (DNA ...
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bioRxiv - Biochemistry 2023Quote: The tendency of the protein to form aggregates was determined by dynamic light scattering (DLS) in the Delsa Max Core (Beckman Coulter). Prior to measurement ...
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bioRxiv - Pathology 2023Quote: ... the proportion of HIV-1-infected cells was determined by intracellular staining of the viral protein Gag-p24 with a Mouse anti-p24-FITC antibody (clone KC57, Beckman Coulter).
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bioRxiv - Neuroscience 2024Quote: ... Biological titers of the viral preparations were assessed by transducing HEK293T cells with serial dilutions and checking the percentage of green fluorescent protein (GFP)-positive cells by flow cytometry (Cytomics FC 500, Beckman Coulter). Hippocampal-cultured neurons were transduced at 7DIV with lentiviral vectors (1-2 transducing units/cell).
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bioRxiv - Molecular Biology 2022Quote: ... were pre-clarified to remove aggregate proteins by spinning at 65,000 rpm for 30 min at 4°C in a TLA100 rotor (Beckman Coulter, Brea, CA) before incubating with F-actin ...
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bioRxiv - Cell Biology 2019Quote: ... The obtained supernatant (containing membrane and cytosolic proteins) was then centrifuged at 180,000 × g for 90 min at 4°C using a TLA-55 rotor (Beckman Coulter, Brea, CA). The obtained pellet (containing intact membranes ...
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bioRxiv - Genomics 2019Quote: ... Membranes and other cellular debris were sedimented away from soluble protein on a Beckman J2-21 large floor centrifuge (Beckman Coulter, Brea, CA). Supernatant containing the recombinant protein was incubated with 10 mL of precleared Ni-NTA beads for 10min at 4°C ...