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Citations for Beckman :
1 - 30 of 30 citations for Hydroxy PEG4 t butly ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: We characterized the presence of T and B cell subpopulations using DuraClone IM T cells (Beckman Coulter; #B53328) and DuraClone IM B cell tubes (#B53318 ...
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bioRxiv - Immunology 2021Quote: ... Residual PE-Cy7+ cells were excluded and EYFP+PE+ (CD4+GzB+ T cells) or PE+CD4+ (Ifng-/-CD4+ T cells) were sorted using MoFlo cell sorter (Beckman Coulter/Dako-Cytomation). CD4+GzB+ T cells (2.8 x 105 cells/animal ...
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bioRxiv - Molecular Biology 2020Quote: ... T cells were collected and subjected to FACS (Beckman Coulter, Brea, CA, USA) using the Cell Quest software (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... 72h later CAR-T cells stained with CFSE were analyzed by CytoFLEX (Beckman) with FlowJo 7.6 software.
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bioRxiv - Immunology 2022Quote: ... Human Vγ9Vδ2 T lymphocytes were phenotyped using PE-labeled anti-NKG2A (#Z199, Beckman Coulter), PE-labeled anti-NKG2D (#ON72 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... mRNA was purified from total RNA using poly- T oligo- attached magnetic beads (Beckman Coulter). Fragmentation was carried out using divalent cations under elevated temperatures in NEBNext First Strand Synthesis Reaction Buffer (5X) ...
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bioRxiv - Bioengineering 2024Quote: ... Primary T cells were counted using the ViCell Blu automated cell counter (Beckman Coulter, Canada) and maintained at 0.25 - 1 x 106 cells / mL by daily addition of complete culture media ...
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bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cells were sorted into separate tubes (MoFlow, Beckman Coulter, Brea, CA, USA). A purity of > 98.0% was achieved as monitored by reanalysis of the sorted samples.
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bioRxiv - Immunology 2022Quote: ... S1PR1 expression in CD4+ T cells was assessed by flow cytometry using CytoFlex S (Beckman Coulter).
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bioRxiv - Cell Biology 2024Quote: ... Platelet concentration was measured with the Beckman Coulter T-540 (Beckman Coulter Inc., Brea, CA, USA) hematology analyzer and adjusted to 250 × 106 per mL with Tyrode-HEPES buffer supplemented with 1 mM MgCl2 ...
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bioRxiv - Immunology 2022Quote: Human Vγ9Vδ2 T lymphocytes were stained with FITC-labeled anti-human TCR Vδ2 mAb (#IMMU389, Beckman Coulter) and APC-labeled anti-human CD3ε mAb (#UCHT1 ...
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bioRxiv - Cancer Biology 2024Quote: ... T cell migration to the lower chamber was quantified using a CytoFLEX flow cytometer (Beckman Coulter, USA).
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bioRxiv - Immunology 2024Quote: ... CD4 and CD8 h1928z-2A-DsRed CAR T cells were sorted using the CytoFLEX SRT (Beckman Coulter) into 200 uL TRIzol LS (Invitrogen # 10296010 ...
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bioRxiv - Cancer Biology 2021Quote: ... The concentration of the expanding human T cells was calculated on a Coulter Multisizer (Beckman Coulter, Brea, CA) and maintained at 1.0-2.0 × 106 cells per mL in R10 media (RPMI-1640 plus GlutaMAX-1 ...
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bioRxiv - Cancer Biology 2021Quote: ... CAR expression and activation/differentiation of the expanding T cells were monitored by flow cytometry (CytoFLEX cytometer, Beckman Coulter) twice a week (see further) ...
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bioRxiv - Zoology 2021Quote: ... Blood samples were also collected in EDTA for leucocyte counts immediately after collection on a Coulter T-890 (Beckman). EDTA blood ...
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bioRxiv - Cancer Biology 2022Quote: T cell sub-population profiling studies on days 14 and 21 were analyzed using CD3-PC7 (Beckman Coulter, 6607100), CD4-APC-A700 (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: ... human Vγ9Vδ2 T lymphocytes were collected and stained with a FITC-labeled anti-human TCR Vδ2 mAb (#IMMU389, Beckman Coulter) and analyzed by flow cytometry ...
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bioRxiv - Immunology 2024Quote: In functional assays proportion of infected CD4+ T cells was address by intracellular staining of p24 (KC57, Beckman Coulter) in combination with anti-CD3 ...
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bioRxiv - Immunology 2024Quote: ... and T-cells (CD45+ CD3+) were sorted into separate tubes using an Astrios Cell Sorter (Beckman Coulter Brea, CA). Distal IEC were removed as described above ...
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bioRxiv - Immunology 2024Quote: ... AML patients and one t(8;21) AML patient-derived xenograft (PDX) sample (RL048)20 by flow cytometry (Cytoflex LX, Beckman Coulter ...
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bioRxiv - Bioengineering 2022Quote: ... The frequency of proliferating T cells was determined after 96 hours by flow cytometry using a CytoFLEX II (Beckman Coulter). Zombie Green (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... TCRVβ14+ CD4+ naïve T cells from spleen and lymph nodes of TCR Tg mice using the MoFlo Astrios or MoFlo Astrios EQ (Beckman) at the Flow Cytometry Core in the UIC Research Resources Center (RRC) ...
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bioRxiv - Immunology 2023Quote: Flow cytometry analysis was performed at the end of the 7 day T cell progenitor differentiation stage using a CytoFLEX LX cytometer (Beckman), and data were compensated using single-stained controls in CytExpert v2.3 ...
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bioRxiv - Immunology 2020Quote: ... during 30min and then transferred into a DuraClone IF T Helper cell tube (CD3, CD4, IL-4, IL-17A, IFNγ) (Beckman Coulter), using the IntraPrep fixation permeabilization kit (Beckman Coulter) ...
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bioRxiv - Cancer Biology 2024Quote: ... CD138 and CD70 for CAR-T (see table of antibodies) and tumor identification and analyzed by flow cytometry using a CytoFLEX Flow Cytometer (Beckman Coulter). Data was analyzed using FlowJo 10.10.0 software ...
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bioRxiv - Immunology 2024Quote: Individual CD24hi or CD24low Vγ1.1+ γδ T cells were isolated from Id3+/+ and Id3-/- mice by flow cytometry and deposited in wells of glass slides (Beckman-Coulter). Vγ1.1 and Vδ6.3 subunits were then amplified by RT-PCR using nested primers in single cells ...
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bioRxiv - Microbiology 2024Quote: ... CD4+ CD25− CD44lo CD62Lhi naive T cells were sorted by FACS using a MoFlo Astrios or MoFlo XDP cell sorter (Beckman Coulter). Naïve CD4+ T cells were cultured in Iscove’s Modified Dulbecco’s medium supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2024Quote: ... the reaction was added 1 µL of 0.2µM HTO primer (5’-GTGACTGGAGTTCAGACGTGTGC*T*C) prior to PCR cycling and cleaned up using SPRISelect magnetic beads (Beckman Coulter, #B23318), 80% ethanol ...
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bioRxiv - Immunology 2022Quote: ... 25 μL of fetal bovine serum and 300 μl R2 Buffer were added to the cell suspension and transferred to DURAClone IF T Activation Tube (Beckman Coulter, USA), incubated in the dark for 45 minutes ...