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Citations for Beckman :
1 - 45 of 45 citations for 27 Carboxy 7 keto Cholesterol d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... FR 3133).Total cholesterol and HDL-cholesterol were assessed using a Beckman Coulter AU analyzer according to the manufacturer instructions (Beckman Coulter ...
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bioRxiv - Bioengineering 2021Quote: ... or 7-Amino-Actinomycin D (7-AAD, Beckman Coulter, B88526) was added to the cell suspensions ...
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bioRxiv - Biochemistry 2024Quote: ... and with 7-Amino-Actinomycin D (7-AAD) Viability Dye (Beckman Coulter). CellTrace Violet was obtained from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... high-density (HDL)- and low-density lipoprotein (LDL)-cholesterol were measured by standard enzymatic methods (CHOD-PAP; Beckman Coulter ...
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bioRxiv - Physiology 2023Quote: ... Plasma cholesterol and triacylglycerol levels were measured using a Beckman Coulter AU480 Chemistry Analyzer (Beckman Coulter, Brea, CA, USA). Glucose and insulin tolerance tests were conducted after 6 h of fasting by intraperitoneal administration of 1 mg/g glucose or 0.75 U/kg insulin in 0.2 % wt/vol BSA in PBS ...
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bioRxiv - Microbiology 2021Quote: ... incubated samples for 7 min, centrifuged (650 x g, 7 min, RT) and measured them by flow cytometry (Cytomics FC500, Beckman Coulter, USA) after resuspension in Tyrode’s buffer ...
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bioRxiv - Biochemistry 2024Quote: In 7 x 20mm Thickwall Polycarbonate tubes (Beckman Coulter), paclitaxel-stabilized MTs were mixed at room temperature with Kif5b proteins in BRB80 plus indicated the nucleotides or metal salts ...
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bioRxiv - Microbiology 2021Quote: ... 27 cycles were used for PCR amplification and cDNA was purified using Agencourt AMPure XP beads (Beckman Coulter) using 15 μl of elution buffer (Takara) ...
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bioRxiv - Cell Biology 2024Quote: As previously described 27 inserts for thick-walled polycarbonate ultracentrifuge tubes (32 ml capacity, Beckman Coulter, Brea, CA) were prepared by bonding air plasma-treated round 18 mm diameter glass coverslips to polydimethylsiloxane (PDMS ...
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bioRxiv - Cell Biology 2021Quote: ... with or without 5µM Gli for 15 minutes at room temperature in 1x BRB80 supplemented with 0.1 mg/mL BSA and then subjected to sedimentation by ultracentrifugation at 50,000 rpm for 10 minutes at 27°C in a TLA 120.1 rotor (Beckman Coulter). Pellets were subsequently resuspended and the amount of protein in each pellet and supernatant was analyzed by SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: 54 µL of media was dispensed into wells (in two 27 µL steps) using a BioMek FX (Beckman-Coulter). Drugs ...
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bioRxiv - Microbiology 2024Quote: The extracted gDNA was sheared 30 times with a 27 G syringe needle and purified using 0.7x AMPure XP beads (Beckman Coulter Inc. ...
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bioRxiv - Molecular Biology 2024Quote: ... 14 µl and 7 µl of SPRIselect Beads (Beckman Coulter) were used in the first and second steps ...
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bioRxiv - Molecular Biology 2024Quote: ... 14 µl and 7 µl of SPRIselect Beads (Beckman Coulter) were used in the first and second steps ...
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bioRxiv - Cell Biology 2022Quote: ... Serum cholesterol and triglycerides were measured by the clinical laboratory of Nanjing Drum Tower Hospital using an autochemical analyser (Beckman Coulter AU5421, CA).
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bioRxiv - Neuroscience 2023Quote: ... 7-color cell sorter using Summit software (Beckman Coulter Life Sciences). The sorted RFP+ only and RFP+/GFP+ cell populations were collected and replated on coverslips with pre-seeded HA cells in NDM ...
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bioRxiv - Cell Biology 2021Quote: ... Apoptosis was analyzed using Annexin V-FITC / 7-AAD kit (Beckman Coulter) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Homogenates were layered on top of 1 ml of 0.85 M sucrose in 2 mM EGTA and centrifuged at 75,000 g for 30 min in a Beckman FW-27 rotor (Beckman, Fullerton, CA, USA). This step was repeat twice ...
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bioRxiv - Microbiology 2022Quote: ... The V4 region of 16S rRNA gene was amplified using the universal primer set 515F and 806R.27 PCR products were cleaned up with an AMPure XP kit (Beckman Coulter, CA), followed by electrophoresis on 0.7% agarose gel and 1.15% Synergel (Diversified Biotech ...
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bioRxiv - Cell Biology 2020Quote: ... The mixtures were loaded into ultracentrifuge tubes (7 x 20 mm, Beckman Coulter) and fractionated at 100,000Xg using a Beckman TL-100 ultracentrifuge ...
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bioRxiv - Cancer Biology 2022Quote: ... GFP+7-AAD- cells were sorted using MoFlo XDP Cell Sorter (Beckman Coulter) or FACSAria III Cell Sorter (Becton Dickinson) ...
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bioRxiv - Cell Biology 2020Quote: ... PEG was removed via centrifugation at 136,000 xg for 7 minutes in TLA100.3 (Beckman Ultracentrifuge), and supernatant was pre-cleared by incubating with Protein A Sepharose beads (GE LifeSciences #17127901 ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were transferred to 7 x 20mm polycarbonate thick-wall tubes (Beckman Coulter, Krefeld, Germany) for ultra-centrifugation (centrifuge Optima TLX with rotor TLA 100 ...
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bioRxiv - Cancer Biology 2023Quote: ... After 5-7 days cells were counted using a Z1 Coulter Particle Counter (Beckman Coulter).
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bioRxiv - Cell Biology 2024Quote: ... The gradients were prepared in 7 x 20 mm centrifuge tubes (Beckman Coulter, Cat#343775) by successively layering 40 µL each of 25% ...
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bioRxiv - Immunology 2023Quote: ... lysates were fractionated on a linear sucrose gradient (7–47%) using the SW-41Ti rotor (Beckman Coulter) at 221,633x g for 2lllhours at 4 °C ...
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bioRxiv - Biophysics 2024Quote: ... and then centrifuged at 250,000 g for 7 min at 37 °C in a TLA100 rotor (Beckman). The pellet was then resuspended in a bright tubulin mix containing 7.5 mM unlabeled tubulin ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound protein was separated from liposomes by transferring 100μl of this mixture to 7×20mm PC ultracentrifuge tubes (Beckman) and carefully layering an equal volume of 0.75M sucrose buffer followed by 25μl of sucrose-free HK buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Fractions were washed by diluting with 7 mL of PBS and ultracentrifuged at 100,000 x g (Type 70.1 Ti rotor, Beckman Coulter) for 2 h ...
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bioRxiv - Cancer Biology 2022Quote: ... was removed using right-side size selection with AMPure XP beads at a 1:1 and 7:1 bead:sample ratio (Beckman Coulter) described previously26 ...
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bioRxiv - Genomics 2020Quote: ... PCR was performed (7 cycles) with standard Illumina paired-end primers and the amplicons were then purified with AMPure XP beads (Beckman). The length ...
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bioRxiv - Immunology 2023Quote: Flow cytometry analysis was performed at the end of the 7 day T cell progenitor differentiation stage using a CytoFLEX LX cytometer (Beckman), and data were compensated using single-stained controls in CytExpert v2.3 ...
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bioRxiv - Biophysics 2024Quote: ... polarity-marked microtubules were pelleted at 20,000 g for 7 min at 37 °C in the TLA100 rotor (Beckman Coulter) and resuspended in BRB80 buffer with 40 mM taxol.
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bioRxiv - Biochemistry 2024Quote: ... Digested lysates were run through 7% - 47% sucrose gradients using a Beckman Coulter ultracentrifuge and SW41 Ti rotor (Beckman Coulter) with 36,000 rpm at 4°C for 2 h ...
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bioRxiv - Plant Biology 2024Quote: ... 400 μL of the extract were loaded on the top of a 7-47 % sucrose gradient and centrifuged in a SW41 rotor (Beckman) for 3h at 38 000 rpm at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... with anti-Met-PE (clone eBioclone 7, eBiosciences) or isotype control (clone eBRG1, eBiosciences) or permeabilized (IntraPrep Permeabilization kit, Beckman Coulter) before staining (total Met) ...
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bioRxiv - Genomics 2023Quote: ... Five million cells were harvested at 315xg for 7 minutes in a 15 mL centrifuge tube (Beckman Coulter Allegra X-14R) and stored at -80°C ...
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bioRxiv - Biochemistry 2024Quote: ... This extract was then carefully layered onto a 7% to 47% linear sucrose gradient and subjected to centrifugation in a SW41Ti rotor (Beckman Coulter) at 36,000 rpm for 2 h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... 96-well plates were incubated at the permissive temperature (15°C) for 7 days and then replicated with a Biomek FX (Beckman Coulter). These replica plates were incubated at the non-permissive temperature (25°C ...
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bioRxiv - Bioengineering 2024Quote: Frozen TGFb1/ET1-treated and untreated hvCTS (n=7 per condition) and hvCOC (n=5 per condition) were lysed using Agencourt RNAdvance Tissue kit (Beckman Coulter) lysis buffer and homogenized at 1000 rpm for 2 min with 5 mm Stainless Steel Beads (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysate was then applied on a continuous 7-47% sucrose gradient (mol weight/volume) in ultra clear tubes (Beckman & Coulter, #331372) and centrifuged at 97,658 x g for 3 h at 4°C using a SW41Ti rotor (Beckman & Coulter ...
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bioRxiv - Biophysics 2022Quote: ... 50 μl of each sample was centrifuged at a speed of 180000Xg (180000 RCF) in 7 mm x 20 mm polycarbonate centrifugation tubes (Beckman Coulter, USA) for at least 2h ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were harvested at 7 400 x g for 30 min in a JLA 9.1000 rotor (Beckman Coulter, Brea, California, USA), washed with 150 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... 2.5 M sucrose in HK was added to the reaction to a final concentration of 1 M (“Input” sample) and transferred to 7 × 20 mm polycarbonate centrifuge tubes (Beckman Coulter cat #343775). HK buffer with 0.75 M sucrose was gently laid on top of the reaction mixture followed by a layer of HK ...
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bioRxiv - Biochemistry 2023Quote: ... lysosomes and mitochondria were enriched by centrifugation of the pooled PNS at 56,500 ×g for 7 min at 4 °C (Beckman-Coulter, 70 Ti fixed-angle rotor). The supernatant was removed ...