Labshake search
Citations for Beckman :
1151 - 1177 of 1177 citations for 1 2 Chloropyridin 3 yl 3 3 dimethylazetidin 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... samples absorbance at 485 nm was measured in a 1 cm quartz cuvette using a DUTM 730 spectrophotometer (Beckman Coulter, Brea, CA, USA). 155 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with an additional 400 µl 1× Binding Buffer and analyzed within 20 min by CytoFLEX S (Beckman Coulter Life Science). At least 1 × 104 cells were analyzed to determine the percentage of apoptotic cells ...
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bioRxiv - Biochemistry 2022Quote: ... The polymerized actin samples were pelleted down by ultracentrifugation at room temperature (45000 rpm for 1 hour, TLA-55 rotor, Beckman Coulter, Indianapolis, IN). The pellets were rinsed once with a 1 mL G-buffer and re-suspended into ice-cold 0.5 mL G-buffer ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Sequencing libraries for each replicate were generated via qPCR and sequencing libraries were purified and size selected using Ampure XP Beads at a 1.8:1 bead volume: library volume (Beckman Coulter, Brea, CA, USA). Library quality and transposition efficiency was accessed using a Fragment Analyzer and PROSize 2.0 (Agilent) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cell lysate was layered on top of a discontinuous OptiPrep® gradient (50 %, 35 %, 30 %, 20 %, and 15 %) and centrifuged at 135,000 g for 1 h at 4°C (Beckman L8-80M Ultracentrifuge, USA). The melanin pellet was recovered ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated for 30 minutes with the following antibodies: anti-HIV-1 core antigen (p24)-RD1 (Beckman Coulter catalog # 6604667), anti-TNFα PE/Dazzle 594 (BioLegend catalog # 502946) ...
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bioRxiv - Cell Biology 2023Quote: ... 2.5 M sucrose in HK was added to the reaction to a final concentration of 1 M (“Input” sample) and transferred to 7 × 20 mm polycarbonate centrifuge tubes (Beckman Coulter cat #343775). HK buffer with 0.75 M sucrose was gently laid on top of the reaction mixture followed by a layer of HK ...
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bioRxiv - Microbiology 2024Quote: ... labelled virus was purified through ultracentrifugation for 1 hour at 35,000 RPM using a rotor SW40Ti (Beckman Coulter Life Sciences, Brea, CA, USA), at 4 °C in a 6–30% OptiPrep (Sigma- Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... The 1 ml fraction containing the phage was then placed on a 40% (w/v) - 60% (w/v) sucrose gradient for 1 hour at 63,400 x g at 4°C (Beckman Coulter SW 40 Ti). The 1 ml fraction was then placed in a 50 kDa dialysis membrane (Spectrum Labs ...
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bioRxiv - Biophysics 2024Quote: ... The cell lysate was centrifuged at 4 °C at 35000 rpm for 1 hour with a Beckman OptimaTM L-80XP Ultracentrifuge equipped with a Ti45 rotor (Beckman, Brea, CA, USA). The pellet containing membranes and cell debris was discarded ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed in FACS buffer and fixed in 1% formalin before being suspended in FACS buffer and analyzed using a BD LSR II FACS system and Cytoflex (Beckman Coulter, Memorial University). Cell populations were gated based on viability dye and subjected to forward and side scattering to identify single cell population before additional analysis using Flowjo (BD Biosciences).
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bioRxiv - Biochemistry 2024Quote: ... Lysates were centrifuged at 26,000 xg for 25 min (Sorvall RC 5B Plus) followed by membrane isolation through ultracentrifugation at 55,000 pm for 1 h (Beckman, Type 70 Ti Rotor). Membranes were resuspended in HBS by mortar and pestle at 20 mL/g and snap-frozen in LN2 before storage at -80 °C until further use ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were centrifuged at 136,000 x g for 1 hour at ambient temperature using a Beckman Coulter Optima MAX-XP Ultracentrifuge with a TLA-55 rotor (Beckman Coulter, CA, USA). The resulting sarkosyl-insoluble pellet was washed in homogenization buffer containing 1% sarkosyl ...
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bioRxiv - Biochemistry 2024Quote: ... The A2aR-expressing membrane fraction was obtained by ultra- centrifugation at 100,000 g for 1 hour at 4°C in a Type 70 Ti Fixed-angle rotor (Optima XPN ultracentrifuge, Beckman Coulter Life Sciences). The pellet (membrane fraction ...
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bioRxiv - Molecular Biology 2021Quote: The VHH-coding regions within parent libraries and 1-round target-enriched sublibraries were PCR amplified and purified using AMPure XP beads (Beckman Coulter, High Wycombe, UK). Then ...
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bioRxiv - Microbiology 2022Quote: ... Reaction products were verified on a 1% agarose gel and purified using the AMPure XP magnetic Bead protocol (Beckman Coulter Inc., Brea, CA, USA). PCR2 was performed in a 25 μl reaction ...
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bioRxiv - Systems Biology 2020Quote: ... and the resultant supernatant was ultracentrifuged at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2; Beckman Coulter 343778 1 ml uncapped tubes).
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bioRxiv - Physiology 2021Quote: ... sEV were further isolated from lEV-depleted supernatants following a 100,000 xg ultracentrifugation step for 1 hour at 4°C (rotor MLA-50, Beckman Coulter Optima MAX-XP Ultracentrifuge) and two washes in NaCl before resuspension in NaCl.
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bioRxiv - Neuroscience 2023Quote: ... PI (1 μl/100 μl) was added 5 minutes before starting the reading on the flow cytometer CytoFlex S (Beckman Coulter, Brea, CA, USA). Results were shown and analyzed through CytExpert flow cytometry analysis software.
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bioRxiv - Biophysics 2023Quote: ... Cell debris was removed by centrifugation at 20,000 rpm at 4°C for 1 h (Avanti® J-E centrifuge from Beckman Coulter, rotor: JLA 25.50). The supernatant was collected and loaded onto a pre-equilibrated 5 ml His-Trap FF crude column ...
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bioRxiv - Bioengineering 2023Quote: ... and a humidity of 85 % in 48-well Flowerplates containing 800 µL defined CGXII medium with 0.5 % (w/v) glucose inoculated to an OD600 of 1 by using the BioLector microbioreactor for 24 h (Beckman Coulter Life Sciences, Aachen, Germany). Increasing concentrations of ANT (final concentration of 0 ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were spun for 1 hour at RT in a Beckman ultracentrifuge using SW 120 Ti rotor (Beckman Coulter, Inc., Brea, CA, USA) at 100,000xg ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed with 1 ml flow buffer once then resuspended in 200 μl flow buffer and measured using a CytoFLEX cytometer (Beckman Coulter, Jersey City, NJ).
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bioRxiv - Biochemistry 2024Quote: ... The samples were then subjected to high-speed ultracentrifugation (100000 g for 1 h) at 20 using an Optima TL-100 benchtop ultracentrifuge (Beckman Coulter, Indianapolis, IN, USA). AgADFs and α-actin alone were processed identically as a controls ...
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bioRxiv - Microbiology 2024Quote: ... was then added to the labelling reaction and incubated for additional 30 min at room temperature before labeled-virus purification through ultracentrifugation for 1 hour at 35,000 RPM using a rotor SW40Ti (Beckman Coulter Life Sciences, Brea, CA, USA), at 4 °C in a 6–30% OptiPrep (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... Protein expression was induced with 0.4 mM Isopropyl β-D-1-thiogalactopyranoside and cells were grown at 37 °C overnight before harvesting by centrifugation at 4000 rpm (Beckman Coulter Avanti JXN-26; JLA8.1 rotor) at 10 °C for 10 min.
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bioRxiv - Microbiology 2024Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb or using PE of FITC-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution). Cell-surface staining of infected primary MDMs cells was performed five days post-infection ...