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Citations for Beckman :
451 - 500 of 531 citations for 5 2 Chloronicotinoyl 2 furoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... brought up to 3 ml using sterile 20 mM Tris-HCl pH 7.5 and pelleted at 40,000g and 4°C for 60 minutes (TLA100.3 rotor, Optima Max-XP ultracentrifuge, Beckman Coulter, Inc.). Finally ...
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bioRxiv - Immunology 2022Quote: ... 1.5 μl of PI was also added and samples were run on the Gallios™ Flow Cytometer (Beckman Coulter, Inc). Data was analysed offline using Kaluza® Flow Analysis software version 2.1a (Beckman Coulter Inc. ...
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bioRxiv - Molecular Biology 2020Quote: ... The following day the viral pellet was resuspended in 5 ml PBS and centrifuged at 20,000 rpm for 90 mins at 4°C (Optima XL-100K, Beckman Coulter). The pellet was then resuspended in the required volume of PBS before being aliquoted and stored at −70°C.
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bioRxiv - Immunology 2021Quote: ... subsequently overlaid onto 5 mL of 20% sucrose and ultracentrifuged at 24,000 rpm for 2.5 hrs (Beckman XPN80, SW32.Ti). The viral pellet was resuspended in PBS.
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bioRxiv - Microbiology 2021Quote: ... bacterial cells were washed in PBS and diluted 1:5 before acquisition on a CytoFLEX benchtop flow cytometer (Beckman Coulter). For each sample ...
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bioRxiv - Immunology 2021Quote: ... 1×106 thawed PBMCs were centrifuged (750×g for 5 minutes at 4°C) using a swinging bucket rotor (Beckman Coulter Avanti J-15RIVD with JS4.750 swinging bucket ...
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bioRxiv - Genomics 2023Quote: ... The suspension was incubated on ice for 5 min and centrifuged at 20,000 ×g (10 min, 4 °C, JA-20 rotor, Avanti J-26 XP, Beckman Coulter). The pellet was re-centrifuged for 5 min and both supernatants were combined into a new tube ...
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bioRxiv - Neuroscience 2022Quote: ... trypsin-dissociated cells on 96-well plates were centrifuged at 1,000g for 5 min at 4°C using a swinging-bucket rotor for microplates (Beckman S6096) in an Allegra X-30R centrifuge (Beckman) ...
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bioRxiv - Cancer Biology 2024Quote: ... The fragments were repaired the 3’and 5’overhangs using End repair mix (component of SureselectXT) and purified using Agencourt AMPure XP Beads (Beckman). The purified fragments were added with ‘A’ tail using A tailing Mix (component of SureSelectXT ...
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bioRxiv - Microbiology 2023Quote: ... Sample analysis was performed by mixing 5 µl of fluorescent vesicles with 20 µl of Flow-Count Fluorospheres (Beckman Coulter) in a final volume of 200 µl of PBS to determine absolute counts ...
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bioRxiv - Cell Biology 2024Quote: ... The step gradients were centrifuged for 5 h at 132.000 x g at 4 °C (SW60 Beckman and Coulter, # 335649). Subsequently ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysates were then loaded onto a 5-50% sucrose density gradient and centrifuged in an SW41 Ti rotor (Beckman) at 36,000 rpm for 2 h at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysed cells were centrifuged and the viscous supernatant was immediately loaded on a 5-24% (w/v) sucrose density gradient and centrifuged in SW 32 Ti rotor (Beckman) overnight ...
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bioRxiv - Microbiology 2023Quote: Human bladder cell line PD07i was cultured at 37°C with 5% CO2 to confluency as previously described and counted using a Coulter Counter (Beckman) to a minimum of 1×105 cells/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysates were sheared with a 25-gauge 5/8 needle and loaded onto CsCl solution (150% w/v) for ultracentrifugation in NVT 65.2 rotor (Beckman coulter) at 42,000 rpm for 20 hours at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Concentrated chromatin samples were loaded on 11 mL gradients of 5-25% sucrose in TE buffer and centrifuged in a preparative ultracentrifuge with a SW41 Ti rotor (Beckman) for 11 hrs ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were centrifuged at 1500 g for 5 min in an F2402 rotor in a GS15R table-top centrifuge (Beckman) and resuspend with water to wash the cells ...
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bioRxiv - Biophysics 2023Quote: ... Each 300μl dialysate was mixed with 300μl of 80% (w/v) Nycodenz dissolved in reconstitution buffer and then divided equally into two 5 x 41 mm ultracentrifuge tubes (Beckman). Then ...
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bioRxiv - Cancer Biology 2024Quote: ... The lysates were then loaded onto a 5–50% sucrose density gradient and centrifuged in an SW41 Ti rotor (Beckman) at 36,000 rpm for 2 h at 4 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... The samples then underwent a second Zymo DNA Clean & Concentrator-5 purification and were eluted in 30µl of elution buffer prior to a 1.2X AMPure XP bead (Beckman Coulter; A63881) cleanup ...
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bioRxiv - Plant Biology 2020Quote: MP of pollen protoplasts was assessed by staining with 1 µM DiBAC4(3) for 5 min (Breygina et al. 2010) on Gallios flow cytometer (Beckman Coulter), analysis was performed with FlowJo software (TreeStar) ...
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bioRxiv - Neuroscience 2021Quote: ... 4 × 5-s bursts) and subsequently centrifuged at 48,000g at 4°C (Beckman Avanti J-251 Ultracentrifuge; Beckman Coulter, Fullerton, CA) for 30 min ...
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bioRxiv - Biophysics 2021Quote: ... transferred to 15 mL conical tube and centrifuged at 233 x g for 5 min at 22 °C using a swinging bucket rotor (Beckman Coulter). Pelleted cells were resuspended and washed once with 1x PBS (-CaCl2 ...
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bioRxiv - Cell Biology 2021Quote: ... OptiPrep top to bottom density gradients 5 % to 40 % were performed as described66 in 12mL open top polyallomer tubes (Beckman Coulter). 1mL fractions were collected from the top ...
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bioRxiv - Biophysics 2021Quote: ... and the fractions containing the protein of interest were combined and concentrated to 2mL at a final concentration of 3.85 mg/mL by repeated centrifugation in an Amicon Ultra Centrifugal Filter at 4k rpm for 5 min at 4 °C (Beckman SX4250). The concentrated protein was incubated overnight with TEV (1:50 mass ratio ...
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bioRxiv - Plant Biology 2022Quote: ... diluted four times with 5 mM HEPES-KOH pH = 7.5 and centrifuged at 21,5000 rpm for 20 min (Beckman Coulter JA 25.50).
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bioRxiv - Neuroscience 2020Quote: ... A sucrose density gradient was prepared with 5 ml of both 0.8 M and 1.2 M sucrose in thin wall ultracentrifugation tubes (Beckman Coulter, Cat # 344059). P2 homogenate was mixed with 1 ml of homogenizing buffer ...
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bioRxiv - Microbiology 2022Quote: ... Pellets were resuspended in 5 ml SM buffer and purified via CsCl density gradient ultracentrifugation (Optima XPN-80 ultracentrifuge; Beckman Coulter) at 19,200xg for 18 h at 10°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were brought to a final volume of 300 μl if necessary with lysis buffer and loaded on a non-linear sucrose gradient prepared by layering different percentage of sucrose solutions (5%, 34% and 55%) into an Open-Top Thinwall Ultra-Clear Tube (Beckman, 344059). A 5x gradient buffer (100 mM HEPES pH 7.6 ...
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bioRxiv - Biochemistry 2020Quote: ... cells were centrifuged at 4 ℃ for 5 minutes at 300g and suspended in HBSS and analyzed with a flow cytometer (CytoFLEX, Beckman Coulter). Mean fluorescence intensity was calculated for each group.
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bioRxiv - Biochemistry 2021Quote: ... live cells were stained with Alexa 647-conjugated M1 anti-FLAG antibody (1:1000, 5 minutes) and analyzed on a Cytoflex flow cytometer (Beckman Coulter).
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bioRxiv - Immunology 2021Quote: ... Insoluble proteins and cell debris were removed by centrifugation (13,000 rpm, 30 minutes at 5°C, JA-30.50Ti rotor, Beckman, Palo Alto, CA). The soluble CuMVTT CP protein in clarified lysate was pelleted using saturated ammonium sulfate (1:1 ...
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Preferred endocytosis of amyloid precursor protein from cholesterol-enriched lipid raft microdomainsbioRxiv - Neuroscience 2020Quote: ... 1.6 ml of 35% (w/v) sucrose and 5% (w/v) sucrose in MBS were layered in a 5.1 ml ultracentrifuge tube (Beckman Coulter, #326819) to form a discontinuous sucrose gradient ...
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bioRxiv - Molecular Biology 2022Quote: Nuclei were isolated from 200 cotyledons of 5-day-old seedlings and purified using a two-layer Percoll gradient at 3000 g before staining with 0.5 μM DAPI and sorting by FACS according to their ploidy levels using a MoFlo Astrios EQ Cell Sorter (Beckman Culture) in PuraFlow sheath fluid (Beckman Coulter ...
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bioRxiv - Genetics 2023Quote: ... STR DNA fragments were amplified using a pair of specific primers with 5’ terminal parts of P5 and P7 Illumina adapters (Table S1) and purified by 100% (v/v) SPRIselect beads (Beckman Coulter). In the second step ...
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bioRxiv - Immunology 2023Quote: ... The live singlets were counted using DAPI 1:10 dilution by mixing 45 μl of cell suspension with 5 μl DAPI by gating on FSC-A vs FSC-H measuring average event/ μl on Cytoflex (Beckman Coulter). Subsequently ...
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bioRxiv - Cell Biology 2022Quote: ... and the pellet was resuspended with 5 ml buffer B and transferred into a 14 ml ultracentrifuge tube (331372, Beckman Coulter). 9 ml Buffer C (1.8 M sucrose ...
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bioRxiv - Neuroscience 2024Quote: ... were loaded onto 10–50% continuous sucrose density gradients in polysome buffer (20 mM HEPES pH 7.4, 150 mM KCl, 5 mM MgCl2) (Gradient Master™, BioComp) and centrifuged in an SW40Ti swing-out rotor (Beckman Coulter) at 250,000 g for 2 h at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatant was incubated in a boiling water bath for 5 min and centrifuged for 10 min at 40,000 rpm (Beckman Ti45 rotor). Supernatant was supplemented with ammonium sulfate (1 g per 6.1 ml) ...
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bioRxiv - Microbiology 2024Quote: ... incubated at 25°C and 5% CO2 (PF) or 37°C and 5% CO2 (BF) for 60 min and analyzed using a CytoFLEX flow cytometer (Beckman Coulter). Events (10,000 ...
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bioRxiv - Microbiology 2024Quote: ... 0.2% Triton X-100 and 100 µg/mL chloramphenicol) and ultracentrifuged at 42500 rpm for 5 h in type 70 Ti rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... bottom layer was made by diluting OptiPrep™ solution with clarified HM or digesta sample (2.25 mL OptiPrep™ + 0.75 mL sample = 3 mL) into a 5 mL open-top thin wall ultra-clear tube (Beckman Coulter). A discontinuous gradient was made with 40% (w/v) ...
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bioRxiv - Plant Biology 2023Quote: ... for 15 minutes at 20°C followed by 5 minutes at 65°C for inactivation of the enzymes and purified with 1X AMPure XP beads (Beckman Coulter); 1D2 Adapters (ONT ...
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bioRxiv - Cell Biology 2023Quote: ... ice-cold 5 mL 2.5 M sucrose was added to the bottom of an open-top plastic tube (Beckman-Coulter 344058), carefully topped up with ice-cold 5 mL 1.875 M sucrose ...
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bioRxiv - Cell Biology 2022Quote: ... After ultracentrifugation at 200,000 x g for 5 h at 4°C (SW55Ti rotor in a XL-90 Beckman Coulter centrifuge), fractions containing purified EVs or purified phages were extracted from the top following the scheme in Supp ...
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bioRxiv - Molecular Biology 2022Quote: ... The gradients were prepared using freeze-thaw method42 in Thinwall Ultra-Clear ultracentrifuge tubes (5 ml, 13×51 mm; Beckman-Coulter). To create the 50% sucrose cushion ...
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bioRxiv - Microbiology 2022Quote: The purified AdgI/AdgH complex sample was diluted to 0.5 mg/ml using the sample buffer containing 25 mM Tris-HCl pH 7.5 and 150 mM NaCl and subjected to the sedimentation velocity measurements using an Optima XL-I analytical ultracentrifuge (Beckman-Coulter). Data was analyzed with the SEDFIT and SEDPHAT programs33,34.
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bioRxiv - Immunology 2022Quote: ... Cells were pelleted at 400xg for 5 min and washed with PBS twice before flow cytometry (Beckman Coulter CytoFLEX Flow Cytometer). Analysis of phagocytic ability was undertaken using FCS Express.
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bioRxiv - Cell Biology 2024Quote: ... We loaded 2 mL of the homogenate mixture on top of a 3.92 ml 62% sucrose in MAB cushion in an Ultra-Clear 5/8” X 4” Beckman Coulter tube (344061, Beckman Coulter). We added the remaining sucrose in MAB solutions ...
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bioRxiv - Immunology 2024Quote: ... the supernatant was discarded and the cell pellet resuspended in 5 mL complete RPMI for counting on Vi-Cell XR automatic cell counter (Beckman Coulter). After counting ...