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Citations for Diagenode :
1 - 28 of 28 citations for 8 Bromoguanosine hydrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Upon 8 cycles of sonication with Pico Bioruptor (Diagenode) (30sec ON/ 30sec OFF) ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was sheared to 8 kb fragments using Megaruptor 2 (Diagenode). Standard SMRTbell libraries were prepared using Template Preparation Kit 1.0 (Pacific Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates were sonicated with a Bioruptor Pico sonicator (Diagenode, total time 8 min) and 4μg of antibodies were added to sheared chromatin (from 4 million cells for Olig2 and from 1 million cells for histone marks ...
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bioRxiv - Cancer Biology 2022Quote: ... 1mM EDTA) and subjected to 8 cycles of sonication with Pico Bioruptor (Diagenode) (30sec ON/ 30sec OFF) ...
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bioRxiv - Genomics 2020Quote: ... 5 μg of purified DNA was sheared at 8 kb using the megaruptor1 system (Diagenode), followed by a DNA damage repair step on 2 μg of sample ...
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bioRxiv - Cancer Biology 2021Quote: ... Chromatin corresponding to 8 μg of DNA was incubated with anti-H3K9me3 (Diagenode; Cat#: C15410193), anti-H3K27me3 (Diagenode ...
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bioRxiv - Genomics 2021Quote: ... 8 µg of adequately sheared chromatin was subjected to immunoprecipitation using iDeal ChIP-seq kit for Histones (Diagenode, C01010050) with 2 µg of antibodies against histone modifications or 10 µg of custom RPB1 antibody ...
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bioRxiv - Microbiology 2021Quote: ... 4 µg of chromatin were immunoprecipitated overnight at 4°C with 8 µg of antibody against H3K9me3 (Diagenode, C15410193) previously coupled to protein A/G magnetic beads provided in the kit ...
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bioRxiv - Biochemistry 2023Quote: ... and the air-dried pellets were resuspended in 500 μl 8 M urea and sonicated in a bioruptor (Diagenode) with high amplitude for 10 min with cycles of 30 seconds on and 30 seconds off.
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bioRxiv - Genomics 2023Quote: ... 25 ng of RNA in 8 µL water was input to the D-Plex Small RNA kit (Diagenode, C0503000) and libraries were prepared according to the manufacturer recommendations with the exception that 9 cycles of PCR were carried out ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were sonicated with 8 cycles of 30s with 30s intervals at the low setting in a Bioruptor Plus water bath sonicator (Diagenode) and centrifuged for 15 min at 16000 g at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... treated by RNase A (20 µg/mL) for 1h at 37°C in TE buffer and sonicated 8 min with BioRuptor (Diagenode) (15 sec ON / 15 sec OFF ...
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bioRxiv - Physiology 2020Quote: ... Resulting nuclei were centrifuged at 12,000 RPM and resuspended in shearing buffer (1% SDS, 10mM EDTA pH 8, 50mM Tris pH8) and sheared in 1.5ml Bioruptor Microtubes (Diagenode, C30010016) in a Bioruptor (Diagenode ...
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bioRxiv - Molecular Biology 2019Quote: ... mixed by rotation at 4°C for 30 min, and then sonicated for 8 cycles (ON for 30 s, OFF for 30 s) using a Picoruptor Sonicator (Diagenode). Each tube of lysate was incubated overnight at 37°C in hybridization buffer (750 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... ChIP from S2 cells was carried out using the same protocol from 2 x 107 cells with a modification to the chromatin preparation procedure to sonicate chromatin for 8 cycles of 30s on/30s off using a Bioruptor Pico (Diagenode). Recovered DNA was quantified on an Agilent TapeStation System using a High Sensitivity DNA ScreenTape (Agilent) ...
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bioRxiv - Microbiology 2019Quote: ... washed cell pellets were lysed in 50 mM HEPES pH 8 with 5% SDS followed by 10 min (30 sec on/off) sonication in a Bioruptor Pico sonicator (Diagenode) at 18 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... The nuclei were suspended in the shearing buffer and sonicated for 8 cycles of 30sec on/off using Bioruptor Pico (Diagenode). The sheared chromatin was reverse-crosslinked and DNA fragment size was ensured by Agilent TapeStation ...
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bioRxiv - Cell Biology 2023Quote: ... and lysed by sonication at high power for 8 cycles (15 sec on, 60 sec off) using a Bioruptor (Diagenode). The lysates were collected after centrifugation at 13,000 rpm for 10 min at 4 °C and the supernatants were prepared for nuclear protein analysis ...
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bioRxiv - Neuroscience 2023Quote: ... and 8 μl was used for small RNA libraries construction by the D-Plex Small RNA-seq Kit (Diagenode C05030001). Indexes were attached using the D-Plex Single Indexes for Illumina - Set A (Diagenode C05030010 ...
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bioRxiv - Immunology 2024Quote: ... Nuclear extracts were prepared 21 and chromatin DNA was sonicated using Bioruptor Pico (Diagenode, 8 cycles, 30s on/off bursts) to generate DNA fragments ∼200-700 bp ...
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bioRxiv - Developmental Biology 2024Quote: ... were sonicated 8 pulses (30 seconds ON / 30 seconds OFF) to 200–500 bp fragments using a Bioruptor Pico sonicator (Diagenode) in 200ul of L3 buffer ...
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bioRxiv - Microbiology 2020Quote: ... was added to the lysate and incubated at 37 °C for 10 min before sonication (2 × 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Microbiology 2022Quote: ... were added to the lysate and incubated at 37 °C for 10 min before sonication (2 x 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Biochemistry 2022Quote: ... Crosslinked proteins were denatured in 8 M urea 50 mM Tris and sonicated for 15 cycles of 30 s with a Bioruptor Plus (Diagenode, SA) at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... were added to the lysate and incubated at 37°C for 10 min before sonication (2 x 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Genomics 2020Quote: ... Purified DNA was sonicated to obtain fragments of an average size of 300-400bp using a Bioruptor Pico (Diagenode; 8 cycles; 20s on and 60s off). 3μg of sonicated DNA was used for library preparation ...
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bioRxiv - Neuroscience 2024Quote: ... The nuclear pellets were lysed with lysis buffer (50 mM Tris-HCl, 10 mM EDTA, 1% SDS, pH 8) and then sonicated with Bioruptor (Diagenode, 15 cycles, 30s ON/30s OFF). The size of the DNA in the sheared chromatin fragments was checked before precipitation by a Tapestation system (Agilent 4200 ...
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bioRxiv - Cancer Biology 2020Quote: ... Purified DNA was fragmented to obtain fragments of an average size of 300–400 bp using a Bioruptor Pico (Diagenode; 8 cycles; 20 sec ON/60 sec OFF). 3 μg of DNA per condition were used for library preparation ...