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Citations for Hampton Research :
1 - 44 of 44 citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... crystallization was performed using a 9-well glass plate and sandwich box setup (Hampton Research), and the reservoir solution consisted of 1.9 M potassium phosphate adjusted to pH 7.8 by varying ratios of KH2PO4 and K2HPO4 ...
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bioRxiv - Biophysics 2023Quote: ... for the human CPEB3 complex and PEG/Ion screen (Hampton Research) for the chimpanzee complex ...
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bioRxiv - Biophysics 2020Quote: ... Crystallization trials were performed in three well high throughput crystallization plates (Swissci, Hampton Research, Aliso Viejo, CA, USA) using commercial crystallization screens (Hampton Research ...
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bioRxiv - Biochemistry 2020Quote: ... Crystallization optimization was performed by hanging drop vapor diffusion after high-throughput screening identified suitable starting conditions (PEG-Ion HT, condition C11; Hampton Research). For the initial matrix screening ...
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bioRxiv - Molecular Biology 2021Quote: ... Screening of crystallization conditions was performed using commercial high-throughput screening kits available in deep-well format from Hampton Research (Aliso Viejo, CA) or Qiagen (Germantown ...
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bioRxiv - Biophysics 2023Quote: ... CjLas1-Grc3 complex and CjLas1 truncated protein (HEPN domain) were first obtained using the sitting drop vapor diffusion method using high-throughput crystallization screening kits (Hampton Research, Molecular Dimensions and QIAGEN). Crystals were then grown in a mixed solution containing 1 μl complex solution and 1 μl of reservoir solution using the hanging drop vapor diffusion method at 16°C ...
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bioRxiv - Biochemistry 2022Quote: ... 8 ml of 1:1 paraffin oil: silicone oil (Hampton research) was poured before adding 2 μl of protein and 2 μl of the crystallization condition to each well ...
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bioRxiv - Biochemistry 2022Quote: ... and 20-26% PEG3350 combined in a 1:1 or 2:1 ratio plated on Greiner 72-well micro-batch plates (Hampton Research) under paraffin oil (EMD Chemicals ...
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bioRxiv - Biochemistry 2024Quote: ... at 1:1 sample to mother liquor ratio in 96-well intelliplates (Hampton research). Initial needle-shaped crystals were formed within 2 to 3 days at 20 °C in presence of 0.2 M Ammonium phosphate monobasic ...
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bioRxiv - Biophysics 2020Quote: ... enzyme were crystallized by mixing 1 μL of enzyme at 1 mM and 1 μL of crystallization solution (17-23% PEG 3350 (Hampton Research, Aliso Viejo, CA, USA) and 0.2 M MgCl2 (JT Baker ...
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bioRxiv - Cancer Biology 2020Quote: ... was reconstituted in DMSO at 4.5□mg□ml−1 and diluted 1:10 in a solution of 5% PEG400 (Hampton Research), 5% Tween80 (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... vapor diffusion crystallizations were set up using a protein concentration of 15-20 mg/ml and a 1:1 ratio of protein:precipitant solution in 24-well sitting drop plates (Cryschem M Plate, Hampton research) with a well solution containing 32-38 % PEG3350 ...
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bioRxiv - Microbiology 2019Quote: ... 1 mM EDTA and 15 mM NaCl) with 1 μL reservoir solution and 2 μL of perfluoropolyether cryo-oil (Hampton research). The crystals were harvested ...
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bioRxiv - Biochemistry 2022Quote: ... 0.1 M MES pH 6.5 at a 1:1 ratio at 16°C were used to prepare a microseed stock with seed beads (Hampton Research). Large-volume crystals of ligand-free Mac1 were grown using a Hampton nine-well sandwich box set up with 150 μl drops of Mac1 (∼19 mg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... 66 were reproduced in hanging drops (1 μL protein solution plus 1 μL mother liquor) using 24-well VDX plates (Hampton Research) containing 500 μL mother liquor in the reservoir ...
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bioRxiv - Bioengineering 2022Quote: ... were set up using Crystal screen 1 (Hampton Research), JCSG-plusTM (Molecular Dimension ...
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bioRxiv - Biochemistry 2023Quote: ... GRAS ScreenTM 1 and GRAS ScreenTM 2 (Hampton Research) were used ...
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bioRxiv - Biochemistry 2022Quote: Crystallization of the FER-ATP/ADP complex was accomplished by using the hanging drop vapor diffusion method by mixing 1 μl of protein complex with 1 μl of reservoir solution in 24-well plates at 291 K (Hampton Research, Aliso Viejo, CA). We successfully obtained crystals with high diffraction quality for the following complexes ...
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bioRxiv - Biophysics 2023Quote: ... pH 6) and 1 μL of Silver Bullets (Hampton Research) reagent [G3 (0.25% 2,2’-Thiodiglycolic acid ...
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bioRxiv - Biophysics 2023Quote: ... pH 6) and 1 μL of Silver Bullets (Hampton Research) reagent [G3 (0.25% 2,2’-Thiodiglycolic acid ...
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bioRxiv - Microbiology 2021Quote: ... 384 conditions were examined using Crystal Screen 1 & 2 (Hampton Research), Index (Hampton Research) ...
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bioRxiv - Biophysics 2019Quote: ... and 1 μL of solubility and stability II kit (Hampton Research, USA), water was added to fill 10 μL per well ...
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bioRxiv - Cell Biology 2022Quote: ... cell pellets were suspended with lysis buffer 1 (50 mM HEPES buffer (Hampton Research), 140 mM NaCl (Nacalai tesque) ...
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bioRxiv - Biochemistry 2024Quote: ... well-formed crystals of Anc4 and Anc5 were obtained using PEGRx 1 (Hampton Research) and further optimized to the condition (15% and 27% (w/v ...
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bioRxiv - Cancer Biology 2024Quote: ... containing 0.1 μL of 1 M Tris-HCl pH 8.3 (Hampton Research, HR2-900-14), 0.024 μL of 5 M NaCl (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... and equilibrated against 500 μL of each precipitant cocktail HR-I (Crystal Screen 1, Hampton Research). Capillary counter-diffusion experiments were set up in capillaries of 0.3 mm inner diameter using the CSK-24 ...
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bioRxiv - Biochemistry 2020Quote: ... Crystals that were obtained in 1-2 days from the Salt Rx HT screen (Hampton Research) condition E10 (1.8 M NaH2PO4/K2HPO4 ...
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bioRxiv - Biophysics 2021Quote: ... Crystals of TMED1 GOLD domain were obtained in the presence of 0.1 M sodium acetate trihydrate pH 4.6 + 8% w/v PEG 4,000 (Crystal Screen 1 #37, Hampton Research), and started to appear after approximately 14 days of incubation ...
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bioRxiv - Biochemistry 2022Quote: ... Crystals were then harvested into 1 µL of the soaking solution in a 96-well Intelli-Plate (Hampton Research) and the drops were sealed ...
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bioRxiv - Biochemistry 2022Quote: Crystallization of DgGH172c sample at 10 mg ml−1 was screened using commercial kits (Molecular Dimensions and Hampton Research) with vapor diffusion sitting drop method ...
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bioRxiv - Microbiology 2020Quote: ... were diluted to a final OD of 0.005 in 1 mL of KBM in a 24-well VDX plate (Hampton Research) and left undisturbed at 30 °C for 120 h ...
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bioRxiv - Biochemistry 2020Quote: ... and the supernatant was set for crystallization screening by using commercially available screening kits (PEGRx™ 1 & 2 (Hampton Research) and Morpheus HT-96 (Molecular Dimensions)) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Purified σ2 receptor was reconstituted into lipidic cubic phase (LCP) by mixing with a 10:1 (w:w) mix of monoolein (Hampton Research) with cholesterol (Sigma Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... and equilibrated against 500 1 µL of each precipitant cocktail of the HR-I & PEG/Ion™ crystallization screening (Hampton Research). Crystallization trials were kept at 293 k in an incubator ...
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bioRxiv - Biochemistry 2021Quote: Samples were incubated with trypsin for different length of time at a molar ratio of 1:6 (enzyme:substrate) following the Proti-Ace™ Kit (Hampton Research) recommendation ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing the P1131-197:Fab P3.2 complex were pooled and concentrated to 10 mg mL−1 as appropriate for crystal formation based on use of pre-crystallisation kit (Hampton Research). Crystallisation trials in 96-well plates were set-up at 4 °C with the sitting-drop method using commercial screens (ProPlex ...
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bioRxiv - Biophysics 2022Quote: ... which were equilibrated against 1 mL of 24% w/v PEG3350 reservoir as described in the Low Ionic Strength Screen handbook (Hampton Research). Drops were set up at both 277 K and 291 K.
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bioRxiv - Biochemistry 2019Quote: ... Methylated T4L-RBCC at 4.5 g L-1 (72 μM) was mixed with an equal volume of reservoir solution optimized from the Index screen (Hampton Research): 15% (w/v ...
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bioRxiv - Physiology 2023Quote: ... Droplets were streaked with a 1:1000 dilution of seed crystals (created using Hampton Research ceramic seed bead kit HR4-781) using a cat whisker ...
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bioRxiv - Biochemistry 2023Quote: ... The purified protein was concentrated to 9 g L-1 (452 µM) and mixed with an equal volume of reservoir solution optimized from the PEG/LiCl Grid Screen (Hampton Research): 14.4% PEG 6K ...
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bioRxiv - Molecular Biology 2022Quote: ... coli TTC-pause at 13 mg/mL was incubated with 3-([3-cholamidopropyl] dimethylammonio)-2-hydroxy-1-propanesulfonate (CHAPSO, 8 mM, final concentration; Hampton Research Inc.) prior to grid preparation ...
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bioRxiv - Microbiology 2023Quote: Crystallization conditions for falcilysin were screened with a protein concentration of 22 mg⋅ml-1 using commercial kits (JCSG-plusTM & Morpheus from Molecular Dimensions; Index & PEG/Ion ScreenTM from Hampton Research) using a mosquito crystallization robot (TTP Labtech) ...
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bioRxiv - Biochemistry 2023Quote: ... Samples containing 4 μM of protein in 50 mM sodium phosphate buffer pH 8.0 were placed in 1-mm optical path cuvette at room temperature in the absence or presence of 1 M trimethylamine N-oxide hydrate (TMAO) (Hampton Research, CA). Ellipticity data were collected for 200-260 nm with a 1 nm step ...
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bioRxiv - Biochemistry 2022Quote: ... Crystallization droplets were prepared by mixing 1 μL protein (YjoB and NTD-1 at 5 mg/mL) with 1 μL crystallization solution under Al’s oil layer in a micro-batch plate (Hampton Research, Aliso Viejo, CA, USA) and then incubated at 20 °C ...