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Citations for Stemcell Technologies :
1 - 44 of 44 citations for QT Nickel Plating Process Agent For Removing Copper Impurity since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Cell plating: Cell plating medium consisted of 95 mL of BrainPhys Neuronal Medium (STEMCELL Technologies, Vancouver, Canada), 2 mL of iCell Neuronal Supplement B (FCDI) ...
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bioRxiv - Cancer Biology 2022Quote: ... To make single-cell suspensions for re-plating, the spheres are chemically dissociated after 7 days of plating, using NeuroCult™ Chemical Dissociation Kit (Mouse, #05707) from Stem Cell Technologies according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... in 9.7 ml NeuroCult Neuronal Plating Medium (Stemcell Technologies, 05713).
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bioRxiv - Bioengineering 2023Quote: ... The cell pellet was resuspended in NeuroCult Neuronal Plating Medium (STEMCELL Technologies, #05713), supplemented with 1:50 NeuroCult SM1 (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... clonogenic frequencies were determined by plating cell lines in Methocult H4434 (StemCell Technologies) in SmartDish meniscus-free 6-well plates (StemCell Technologies) ...
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bioRxiv - Cell Biology 2024Quote: ... The plating media was made up of 200 μl NeuroCult SM1 supplement (Stemcell Technologies, 05711) with 100 μl GlutaMAX 100x (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... 50% of the plating medium were replaced by the BrainPhys™ Neuronal Medium (Stem Cell Technologies) growth medium ...
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bioRxiv - Cancer Biology 2024Quote: ... following by trypan blue exclusion viability test and/or plating in Methocult (Stemcell Technologies cat# 04320) when indicated ...
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bioRxiv - Developmental Biology 2024Quote: ... One day after plating the media was changed to NeuroCult differentiation solution (Stem Cell Technologies 05704) with antibiotic-antimycotic.
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bioRxiv - Microbiology 2021Quote: ... cells were ‘air-lifted’ by removing the media from the apical chamber and replacing the basolateral media with Pneumacult air liquid interface (ALI) media (STEMCELL Technologies) 26 ...
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bioRxiv - Developmental Biology 2022Quote: ... the Matrigel was slowly diluted by removing one ml of Matrigel and adding one ml of StemFlex basal media (STEMCELL Technologies) six times to not expose coating to air ...
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bioRxiv - Immunology 2023Quote: ... for 4-7 days before being switched to ALI culture conditions by removing the apical media and feeding the basal side with PneumaCultTM ALI medium (STEMCELL Technologies). The cultures were incubated 3-4 weeks to achieve mucocilliary differentiation evidence by the presence of mucus and beating cilia.
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bioRxiv - Microbiology 2023Quote: ... ALI conditions were established by removing media from the apical chamber and replacing the media of the basolateral chamber with Pneumacult-ALI maintenance medium (STEMCELL Technologies) supplemented with 4 µg/mL heparin (STEMCELL Technologies) ...
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bioRxiv - Immunology 2024Quote: ... after which they were air-lifted by removing medium in the apical chamber and replacing the basal chamber medium with complete PneumaCult air-liquid interface medium (Stemcell Technologies) + 1:500 Plasmocin ...
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bioRxiv - Cell Biology 2022Quote: ... initiation of the air-liquid interface was performed by removing media from the transwell insert and replacing the expansion media in the lower-chamber with ALI differentiation media (StemCell Technologies). At 48 hour intervals the transwell insert was rinsed with 500 μL PBS to prevent excessive mucous accumulation and the media was refreshed ...
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bioRxiv - Immunology 2023Quote: ... BMDCs were further enriched by isolating loosely adherent cells removing F4/80+ cells then isolating CD11c+ cells by bead purification following manufacturer’s instructions (Stem Cell Tech). Cells were then plated in tissue culture treated dishes in DMEM with 10% FBS then used the following day for experiments.
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bioRxiv - Neuroscience 2022Quote: ... The plating medium consisted of 95 mL of BrainPhys Neuronal Medium (cat. 05790, STEMCELL Technologies, Vancouver, Canada), 2 mL of iCell Neural Supplement B (cat ...
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bioRxiv - Neuroscience 2024Quote: ... at a density of 3.4 x 104 cells/cm2 in NeuroCultTM Neuronal Plating Medium (STEMCELL Technologies, 05713) supplemented with 2% NeuroCultTM SM1 neuronal supplement (STEMCELL Technologies ...
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Metabolic specialization drives reduced pathogenicity in Pseudomonas aeruginosa CF clinical isolatesbioRxiv - Microbiology 2023Quote: ... ALI was established once cells reached full confluency by removing media from the apical chamber and replacing media in the basolateral chamber with Pneumacult-ALI maintenance medium (STEMCELL Technologies, 05001). Pneumacult-ALI maintenance medium was supplemented with Pneumacult-ALI 10x supplement (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... pan CD3+ murine T cells were isolated from single cell splenocyte suspensions of 6 – 12-week-old mice through a negative selection process (STEMCELL Technologies). T cells were activated with αCD3/αCD28 Dynabeads as specified by manufacturer’s instructions in the presence of 100 IU/mL recombinant mouse (rm ...
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bioRxiv - Neuroscience 2023Quote: ... at a density of 30 x 104 cells/well and cultured in NeuroCult neuronal plating medium (Stem Cell Technologies) supplemented with B-27 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Neurons were cultured DIV 0 to DIV 4 in Neurocult™ Neuronal Plating Medium (Stemcell Technologies, catalog no. 05713) with SM1 neuronal supplement (Stemcell Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... The Plating medium was consisted of 1 × BrainPhys Neuronal Medium/N2-A/SM1 kit (BrainPhys; Stem Cell Technologies, 05793) supplemented with 10 μM Y27632 ...
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bioRxiv - Neuroscience 2024Quote: ... the plating medium was removed and replaced by Neurobasal medium supplemented with SM1 supplement (StemCell Technologies; 1:50 dilution), 0.5 mM glutamine and 0.12 mg/mL gentamycin ...
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bioRxiv - Cancer Biology 2022Quote: ... endothelial and haematopoietic lineages in a 3-stage process as described in Figure 1A and the main manuscript text using APEL2 (StemCell Technologies Cat#05275)(61 ...
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bioRxiv - Bioengineering 2023Quote: ... We transition the cultures to ALI by removing the apical medium and replacing the expansion medium with ALI culture media (PneumaCult™-ALI Medium, STEMCELL Technologies, Cat. No. 05001). After 2 weeks of ALI culture ...
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bioRxiv - Microbiology 2024Quote: ... cells were “air-lifted” by removing the media from the apical chamber and replacing the basolateral media with PneumaCult™-ALI-S Medium (ALI) (STEMCELL Technologies, Cat. no. 05050). Medium was replaced in the basal compartment 3 times a week ...
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bioRxiv - Cell Biology 2020Quote: LT-HSCs were isolated and plated in liquid culture with vehicle (0.1%BSA/PBS) or with IGF1 (100ng/ml) for 48 hr before plating in MethoCult GF M3434 (StemCell Technologies) at the indicated numbers and cultured at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... The co-cultures were started by plating 2000 sorted LSKCD150+ to each well in Myelocult medium (M5300, Stem Cell Technologies) supplemented with 10-6 M hydrocortisone (74142 ...
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bioRxiv - Cell Biology 2023Quote: CFU assay was performed at the indicated time points plating 800 cells in methylcellulose-based medium (MethoCult H4434, StemCell Technologies) supplemented with penicillin and streptomycin ...
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bioRxiv - Bioengineering 2024Quote: ... We transition the cultures to ALI by removing the apical medium and replacing PneumaCult™- Ex Plus Medium with PneumaCult™-ALI Medium (STEMCELL Technologies, Cat. No. 05001). The medium is changed every other day ...
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bioRxiv - Immunology 2021Quote: Bone marrow colony forming assay was performed by plating 104 or 5×104 cells on methylcellulose (MethoCult M3434, Stem Cell Technologies). GEMM (Granulocyte/Erythrocyte/Macrophage/Megakaryocyte) ...
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bioRxiv - Cancer Biology 2020Quote: Colony forming cell (CFC) assay for murine cells was performed by plating 1000 cells on methylcellulose (MethoCult #M3434, Stem Cell Technologies). Colony Assay for human CD34+ HSPCs and AML patient cells were performed by plating 10000 cells and 3000 cells respectively on methylcellulose (MethoCult #H4434 ...
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bioRxiv - Cancer Biology 2020Quote: CFC assays from ES and iPS cells from EB or BL-CFC were performed by plating 10×10^3 cells/mL into MethoCult GF (H4435 StemCell Technologies) in triplicate ...
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bioRxiv - Genomics 2020Quote: Kasumi-1-AE-wild-type or Kasumi-1-AE-FKBP cells were treated for 24 hours with 500 nM dTAG-47 prior to plating in methylcellulose (MethoCult H4434, StemCell Technologies). 500 cells were plated in 1.1 mL Methocult supplemented with 500 nM dTAG-47 ...
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bioRxiv - Bioengineering 2022Quote: ... on 1:100 diluted Geltrex (Thermo Fisher)-coated plates with daily medium changes and passaging every 3-4 days with releasing agent ReLeSR (Stemcell Technologies).
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bioRxiv - Cancer Biology 2020Quote: ... Colony Assay for human CD34+ HSPCs and AML patient cells were performed by plating 10000 cells and 3000 cells respectively on methylcellulose (MethoCult #H4434, Stem Cell Technologies). CFU-GM (Granulocyte/Macrophage) ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were plated for single cell clones by plating 1000 cells per 10cm2 dish on Synthemax with 1x CloneR (Stemcell technologies #05888), and single cell clones were genotyped by PCR for correct integration before sequencing both alleles from positive clones.
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bioRxiv - Cancer Biology 2024Quote: ... the spheres were chemically dissociated after seven days of plating, using the NeuroCult Chemical Dissociation Kit (mouse, #05707) from Stem Cell Technologies, according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Primary mouse clonogenic progenitor assays or colony forming units (CFU) assays were performed by plating 5×105 unfractionated nucleated BM cells in methylcellulose M3434 (StemCell Technologies, Vancouver, Canada) in 1 ml duplicates ...
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bioRxiv - Cancer Biology 2020Quote: ... Cryopreserved MDS cells were thawed and cultured in StemSpan™ + cytokines (10ng/ml hIL3, 10ng/ml hSCF, 5ng/ml hIL6) overnight before plating cells in Methocult H4034 Optimum (Stem Cell Technologies) at a density of 1-2 × 104 cells per mL with DMSO ...
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bioRxiv - Developmental Biology 2022Quote: ... by plating them at 125.000 cells/cm2 on Matrigel and growing the cells in STEMdiff™ Neuron Differentiation Kit (Stem Cell Technologies, #08500) medium for one week with daily medium changes.
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bioRxiv - Neuroscience 2024Quote: ... half of the plating medium was removed from each well and replenished with the same volume of BrainPhys™ Neuronal Medium (STEMCELL Technologies, 05792) supplemented with 2% NeuroCult™ SM1 Neuronal supplement ...
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bioRxiv - Synthetic Biology 2024Quote: ... 10,000 cells were plated per 12-well plate well onto 5% biolaminin 521 LN and differentiation started 6 hrs post plating in TeSR-E6 (Stem Cell Technologies, 05946) with CHIR99021 (Tocris ...