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Citations for Stemcell Technologies :
301 - 350 of 1085 citations for 2 Amino 6 chloro 9 3 5 di O p toluoyl beta D 2 deoxyribofurnanosyl purine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... the medium was further supplemented with 100 U/mL recombinant human IL-2 (STEMCELL technologies, Germany). Blood samples were provided by the Institute for Clinical Hemostaseology and Transfusion Medicine ...
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bioRxiv - Cancer Biology 2024Quote: ... and cultured for 1 day in the presence of 100 units of IL-2 (StemCell Technologies) followed by 2 days in T cell medium alone ...
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bioRxiv - Genomics 2021Quote: ... onto Matrigel-coated (1 mg ml-1) 6-well plates 6 using ReLeSR (STEMCELL Technologies). Cells were differentiated to CD43+ hematopoietic progenitors using the StemCell Technologies Hematopoietic Kit (Cat #05310) ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... cells were maintained on 6-well plates in a humidified incubator at 37°C with 5% CO2 and passaged every 3-4 days (at ∼80% confluence) using ReLeSR (Stemcell Technologies), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: Unsorted floating cells were taken from d2-3 of the blast culture and 5×103 cells were seeded in 1 ml MethoCult (M3434 STEMCELL Technologies) per dish ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were harvested anytime between day 9-21 using dispase (StemCell Technologies, cat. no. 07913) and trypsin to dissociate Matrigel and spheres ...
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bioRxiv - Systems Biology 2024Quote: ... APCs were treated with the pro-inflammatory cytokines interleukin 6 protein (IL-6, Stem Cell technologies), or tumour necrosis factor alpha (TNFα ...
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bioRxiv - Bioengineering 2023Quote: ... containing 300 cells and with 10ng/ml IL-3 (+IL-3 treatment) or without IL-3 (-IL-3 treatment) were plated in a well of a SmartDish (StemCell Technologies, Seattle, WA, USA) in duplicates ...
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bioRxiv - Bioengineering 2022Quote: ... Both UC3-4 and UCS-2 hiPSCs were frozen in mFreSR medium (Stem Cell Technologies, Vancouver, Canada) and stored under cryogenic conditions in a vapor phase liquid nitrogen dewar ...
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bioRxiv - Cancer Biology 2020Quote: ... Slides are then stained for 1-2 days in Carmine Alum (Stem Cell Technologies; Catalog No. 07070) at RT ...
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bioRxiv - Bioengineering 2023Quote: ... the media was fully replaced with 2 mL Hematopoietic Media B (STEMdiff Hematopoietic Basal Media (StemCell Technologies) + STEMdiff Hematopoietic Supplement B (200x ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were expanded by culturing in RPMI 1640 with B27 with 2 μM of CHIR99021 (Stemcell Technologies). Media was replaced daily for 6-7 days or until cells reached confluency ...
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bioRxiv - Neuroscience 2023Quote: ... the cell pellet from one 60 mm dish was resuspended in 2 mL mFreSR medium (Stemcell Technologies) to freeze two vials of the cells by putting them in an isopropanol freezing container at –80°C overnight before transferring them into liquid nitrogen.
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bioRxiv - Neuroscience 2024Quote: ... and Lancaster and Knoblich [2] using the commercially available STEMdiff™ Cerebral Organoid Kit from StemCell Technologies. Organoids were generated according to the guidelines from the manufacturer with a few modifications enabling large-scale culturing of appropriate organoid numbers (Figure S1) ...
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bioRxiv - Developmental Biology 2024Quote: ... The EBs were then incubated in 2 mL Gentle Cell dissociation reagent (Stem Cell Technologies, #100-0485) in a 37°C water bath for 15 minutes followed by vigorous trituration until they formed a single cell solution ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were cryopreserved at 2 × 107 cells per ml in CryoStor CS10 cryopreservation medium (StemCell Technologies) until use ...
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bioRxiv - Neuroscience 2022Quote: ... and Neurotrophin-3 (NT-3; 20 ng/ml, Stemcell technologies) and the culture medium was changed every 3-4 days ...
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bioRxiv - Cell Biology 2020Quote: ... Neural rosettes were derived between day 3 to day 5 by addition of STEMdiffTM Neural Rosette Selection Reagent (05832, Stem Cell Technologies) for 45 mins-1 hr at 37oC ...
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bioRxiv - Physiology 2024Quote: ... Cells were utilized between passages 20 and 80 and passaged every 3–5 days at a confluency of 85–100% using Accutase (STEMCELL Technologies, #07920). The cardiac differentiation protocol was performed as previously described 49 ...
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bioRxiv - Cancer Biology 2024Quote: ... To obtain single cells the tissue was centrifuged at 450 x g for 3 min and the cell pellet was resuspended in 1 mL Dispase 5 U (Stemcell Technologies, 07913) with 100 μL of DNase I 1 mg/mL (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 µL thawed organoids were resuspended into 9 mL IntestiCult Human Organoid Growth Medium (Stemcell technologies) and centrifuged at 150 g at 4°C for 5 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 ng/ml H-PDGF-BB (Shenandoah #100-16) and 2 μg/ml heparin (Stem Cell Technologies #07980). All cells were grown in a standard humidified cell culture incubator at 37 °C and 5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... and further expanded in Neurocult NSA with proliferation supplement (StemCell Technologies; 10ng/ml rhFGF-2, 20ng/ml rhEGF) and incubated at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... PBMCs separation was performed within 2 h of blood withdrawal using SepMate™ tubes (StemCell Technologies, BC, Canada) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Cells were incubated in 2% FCS in PBS with 1 mM EDTA with human Fc block (STEMCELL Technologies) and stained with Aqua LIVE/DEAD Fixable Dead Cell Stain Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: Viable BM cells (2×104) were seeded in 1.1 mL Mouse Methylcellulose Complete Media (M3434; Stem Cell Technologies) in uncoated 6 well plates (3471 ...
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bioRxiv - Bioengineering 2023Quote: ... T cells were cryopreserved at 2×107 cells per mL in CryoStor CS10 cryopreservation media (Stem Cell Technologies) until use ...
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bioRxiv - Immunology 2023Quote: ... was used at 2 µg/mL.11,14 Immunocult-ACF Human B Cell Expansion Supplement (BAC, Stem Cell Technologies) was used at 1:50 dilution per manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were minced into ∼1–2 mm pieces before digesting with 1 × Collagenase/Hyaluronidase (STEMCELL Technologies, Catalog #: 07912) and 100 μg/mL DNase I (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... rosette selection was performed by incubating for 2 hours with Neural Rosette Selection Reagent (Stem Cell Technologies, 05832), forcefully dislodging with warm DMEM/F12 ...
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bioRxiv - Bioengineering 2024Quote: ... Cardiomyocyte Isolation Enzyme 2 (Thermo) was diluted at a 1:1000 ratio into Cardiomyocyte Dissociation Medium (STEMCELL Technologies), and the mixture was pre-warmed to 37°C before use ...
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bioRxiv - Developmental Biology 2024Quote: ... it was switched to PXGL medium (N2B27 medium supplemented with 1 μM PD0325901, 2 μM XAV939 [STEMCELL Technologies, 72674] ...
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bioRxiv - Biophysics 2020Quote: ... were grown and maintained at 37°C in hypoxic conditions (3% O2, 5% CO2) in NeuroCult™ NS-A Proliferation medium (STEMCELL Technologies, 05751) supplemented with 1% Heparin Solution (STEMCELL Technologies ...
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bioRxiv - Genetics 2021Quote: ... 100ng/ml human interleukin 6 (hIL-6) (all obtained from EuroBiosciences) and 35nM UM171 molecule (Stem Cell Technologies). Cells were cultured under normoxic conditions ...
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bioRxiv - Genomics 2020Quote: ... 6% v/v lymphoprep (STEMCELL Technologies, Canada), 1M betaine (#B0300-1VL ...
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bioRxiv - Cell Biology 2021Quote: ... 100μg/mL IL-6 (StemCell Technologies 78050), 35nM UM171 (StemCell Technologies 09655 ...
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bioRxiv - Bioengineering 2021Quote: ... 6 μM of CHIR99021 (Stemcell Technologies, 72054) was added on day 0 and left for 24h followed by medium change ...
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bioRxiv - Genomics 2022Quote: ... 6% v/v lymphoprep (STEMCELL Technologies, Canada), 1M betaine (#B0300-1VL ...
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bioRxiv - Biochemistry 2024Quote: ... IL-6 (StemCell Technologies, Cat. No. 78148), and IL3 (StemCell Technologies ...
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bioRxiv - Evolutionary Biology 2020Quote: ... followed by expansion for 9 days in Expansion Media composed of Stemspan H3000 (Stem Cell Technologies, 09850) supplemented with 50μg/ml ascorbic acid (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... Sigma for 2 min at room temperature and then incubated in neutralization buffer supplemented with NH4Cl (0.8%, StemCell Technologies) to remove red blood cells ...
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An in vitro neuronal model replicating the in vivo maturation and heterogeneity of perineuronal netsbioRxiv - Neuroscience 2022Quote: ... the media was replaced with BrainPhys™ medium with 2% (v/v) SM1 and antibiotic-antimycotic (StemCell Technologies #5792). From 14 DIV ...
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bioRxiv - Neuroscience 2022Quote: ... neural induction was initiated using a chemically defined neural base medium (NbM) supplemented with 2 µM Dorsomorphin (StemCell Technologies), 3 µM CHIR990021 (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... with base media consisting of Iscove’s modified Dulbecco’s medium (IMDM) supplemented with 2% human blood plasma (STEMCELL Technologies, #70039), 3% human AB serum (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... and 2 µM A83-01, an inhibitor of TGFβ type I receptors (ALK4, ALK5, ALK7) (Stem Cell Technologies, 72022) for 5 days in vitro to induce embryoid body (EB ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were cultured at ∼1-2 x 106mL in ImmunoCult-XF T cell expansion medium (StemCell Technologies, Canada) supplemented with Penicillin-Streptomycin (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... and were differentiated for 2 days using 100 ng/ml phorbol myristate acetate (PMA) (StemCell Technologies, Cambridge, MA, USA). To study the adhesion dynamics of sickle RBCs under shear flow and hypoxia ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were passaged 1-2 times per week using an enzyme-free reagent (ReLeSR; Stem Cell Technologies, 05872). Cells were checked for mycoplasma contamination every two weeks ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were washed twice with PBS (+ 2%FBS) and with a red blood cell lysis buffer (0.8% NH4Cl, 0.1mM EDTA, STEMCELL technologies). Thereafter ...
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bioRxiv - Biophysics 2023Quote: ... and the media further supplemented with 30 U mL-1 of human recombinant IL-2 protein (STEMCELL Technologies, USA). T cells were incubated in a humidified incubator with 5% CO2 at 37°C and were split to 0.5×106 cells mL-1 when their concentration surpassed 2.5×106 cells per well or when medium became depleted (turning yellow in color) ...