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Citations for Macherey-Nagel GmbH :
1 - 37 of 37 citations for Ion Exchange Chromatography since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... total RNA was separated using anion exchange chromatography with a Nucleobond AX100 column (Macherey-Nagel, Duren, Germany). In short ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified from cell-free lysate by metal ion affinity chromatography with Protino Ni-NTA Agarose (Macherey-Nagel) as matrix followed by ultrafiltration (Amicon Ultra-15 centrifugal filter units ...
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bioRxiv - Molecular Biology 2023Quote: ... and endotoxin-free plasmid DNA was isolated using anion exchange columns (NucleoBond, Macherey-Nagel).
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bioRxiv - Microbiology 2020Quote: ... The PCR product was purified with anion exchange column AX2000 (Macherey-Nagel, Cat. No. 740525) and precipitated by IPA (IBI Scientific ...
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bioRxiv - Biophysics 2021Quote: ... and purified by Ni-IDA-chromatography (Protino, Macherey-Nagel) and anion-exchange chromatography (Resource™ Q ...
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bioRxiv - Plant Biology 2019Quote: ... Samples were then subjected to solid-phase extraction (SPE) in 96-well plates cation exchange columns (Macherey-Nagel HR-XC), pre-conditioned with 500 μl methanol and equilibrated with 500 μl milliQ H2O ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were analyzed via thin-layer chromatography (Polygram CEL 300, Macherey-Nagel) using 0.5 M potassium phosphate (pH 3.5 ...
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bioRxiv - Biochemistry 2022Quote: ... C18 reversed-phase thin layer chromatography (TLC) plates were obtained from Macherey-Nagel. PEI cellulose TLC plates were obtained from EMD Millipore.
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bioRxiv - Microbiology 2022Quote: ... The clarified lysate was subjected to affinity chromatography on Ni2+ agarose resin (Macherey-Nagel) and washed extensively with lysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... Reaction products were visualized by thin layer chromatography (TLC) in PEI cellulose TLC plates (Macherey-Nagel) and separated using 0.75 M KH2PO4 ...
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bioRxiv - Bioengineering 2020Quote: ... Flash column chromatography was performed on silica gel (Macherey-Nagel, Kieselgel 60 M, 0.04 – 0.63 mm). 1H NMR and 13C-APT NMR spectra were recorded on a Bruker AV-400 (400 MHz and 101 MHz for 1H and 13C ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were then purified from bacterial extracts by affinity chromatography using glutathione-conjugated agarose (Macherey-Nagel), followed by cleavage of the GST-tag with PreScission protease ...
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bioRxiv - Plant Biology 2024Quote: ... The soluble protein was first purified by affinity chromatography with Protino nickel nitrilotriacetic acid agarose (Macherey-Nagel) using the lysis buffer but with the imidazole concentration changed to sequential rounds of 20 mM and 250 mM for washing and eluting the protein ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 mM PMSF and purified by immobilized metal affinity chromatography using a 5 ml nickel column (Macherey-Nagel). The protein was eluted from the nickel column with solubilization buffer supplemented with 500 mM imidazole ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins from the soluble fraction were purified by affinity chromatography with Protino Ni-TED PackedColumns2000 (Macherey-Nagel, Düren, Germany) based on the His-tag tail ...
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bioRxiv - Microbiology 2020Quote: ... Lipids were analysed by Thin Layer Chromatography (TLC) on silica gel-coated plates (G-60, 0.25 mm thickness, Macherey-Nagel) developed with CHCl3/CH3OH/H2O (65:25:4 ...
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bioRxiv - Microbiology 2019Quote: ... compounds were separated by reverse-phase chromatography using Ultra Performance Liquid chromatography (UPLC IClass, Waters) using a Nucleodur C18ec column (2.0 mm × 150 mm, 5 μm particle size, Macherey-Nagel). Elution was achieved by increasing the acetonitrile/water (milliQ filtrated on 0.22 μm ...
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bioRxiv - Molecular Biology 2021Quote: ... His6-BsrA was purified from the cell lysate by chromatography on Ni-agarose columns (Protino Ni-TED 1000, Macherey-Nagel) with 300 mM imidazole in phosphate buffer used for elution ...
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bioRxiv - Biochemistry 2021Quote: ... Formed [32P]ATP was separated from the remaining [32P]PPi by thin-layer chromatography (TLC) on polyethyleneimine plates (Macherey-Nagel) in 4 M urea and 750 mM KH2PO4 pH 3.5 ...
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bioRxiv - Zoology 2023Quote: ... Germany) or on a hydrophilic interaction liquid chromatography (HILIC) Nucleodur column (125 × 3 mm, 3 μm, Macherey-Nagel, Düren, Germany) for normalphase chromatography ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Reactions were monitored by thin layer chromatography (TLC) on silica gel (60 F, 0.2 mm, ALUGRAM Sil G/UV254 Macherey-Nagel) by visualization under 254 and/or 365 nm lamp ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The drug concentration in samples was measured with high-performance liquid chromatography (HPLC) using a reverse phase C18 Nucleosil 100-5 (4.0 × 250 mm; 5.0 μm particle size, Macherey-Nagel, Germany) column and mobile phase consisting of MeOH/water (90/10 v/v ...
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bioRxiv - Biochemistry 2023Quote: ... The thin layer chromatography was done with silica gel on aluminum foils (60 Å pore diameter) obtained from Macherey-Nagel and visualized with ultraviolet light (λ = 254 and 365 nm) ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatography of 10 µL of each sample was performed using a Nucleoshell C18 reversed phase column (Macherey-Nagel, Düren, Germany) for human bile acids ...
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bioRxiv - Plant Biology 2019Quote: ... The entire reaction mixture was then loaded onto a thin-layer chromatography (TLC) plate (0.25 mm Silica gel, DC-Fertigplatten, Macherey-Nagel, Germany). The plate was developed with hexane/diethyl ether/acetic acid (80:20:1 ...
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bioRxiv - Cell Biology 2019Quote: ... The lipid was then dissolved in the 1-butanol and separated by two dimension high performance thin layer chromatography (NanoSiL, MACHEREY-NAGEL). For the first dimension ...
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bioRxiv - Bioengineering 2020Quote: ... Reactions were monitored by analytical thin-layer chromatography (TLC) on silica gel plates (Macherey-Nagel, ALUGRAM® Xtra SIL G/UV254) and either visualized by UV light (254 nm ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was transferred to a fresh falcon and directly used for immobilized metal affinity chromatography (IMAC) using the Äktapure25 system with a sample pump (Cytiva) and 1ml Ni-NTA columns (Macherey-Nagel). Proteins were concentrated where necessary using Amicon filter devices with appropriate molecular weight cut-off (Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... The BoNT-X/NTNH complex was purified by gravity-flow affinity chromatography on Ni-NTA agarose resin (Macherey-Nagel, Düren, Germany) in lysis buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... The His6-3C protease was then removed from the sample by immobilized metal affinity chromatography using a 1 mL Protino Ni-NTA column (Macherey-Nagel). The mTRIM66 PHD-bromo-domain protein was further purified by size exclusion chromatography using a HiLoad 16/600 Superdex 75 pg column (Cytiva ...
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bioRxiv - Cell Biology 2020Quote: ... Purification of respective proteins from bacterial extracts was performed by affinity chromatography using Protino glutathione-conjugated agarose 4B (Macherey-Nagel, Düren, Germany). Except for GST-WAVE2-WCA ...
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bioRxiv - Genomics 2021Quote: ... embryos were ground in Tri-Reagent and RNA was purified using affinity chromatography (Nucleospin RNA Clean up kit, Macherey-Nagel, Duren, Germany). Potential contaminating DNA was removed by digestion with rDNase then RNA was purified using Beckman Coulter’s solid-phase reversible immobilization (SPRI ...
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bioRxiv - Biochemistry 2020Quote: ... All performed thin layer chromatography (TLC) was done with silica gel on aluminum foils (60 Å pore diameter) obtained from Macherey-Nagel and visualized with ultraviolet light (λ = 254 and 365 nm) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Extracted steroids were concentrated under nitrogen and dissolved in 20 µl of trichloromethane for separation by thin-layer chromatography on silica gel TLC plates (Macherey-Nagel, Oensingen, Switzerland). Radiolabeled steroids were quantified by autoradiography on a Fuji FLA-7000 PhosphorImager (Fujifilm ...
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bioRxiv - Molecular Biology 2019Quote: ... The clear solution was purified by column chromatography using a His-tag affinity matrix (Protino® Ni-NTA Agarose, Macherey-Nagel GMBH, Düren, Germany). The Vip3A protein was purified using a native protein purification protocol ...
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bioRxiv - Genomics 2021Quote: ... The Analysis for respiratory quinones was carried out by first separating them from other classes using thin layer chromatography on silica gel (Macherey-Nagel Art. No. 805 023), using hexane:tert-butylmethylether (9:1 v/v ...
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bioRxiv - Microbiology 2023Quote: ... DisA was incubated with unlabeled ATP and 15 µl of the reaction product was analysed by thin layer chromatography (TLC; POLYGRAM CEL300 PEI plates) (Macherey-Nagel GmbH & Co. KG, Düren, Germany) using a running buffer of [1 vol ...