Labshake search
Citations for Macherey-Nagel GmbH :
1 - 49 of 49 citations for Collagen Binding Fragment since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... kit with NTB binding buffer (#740595.150, Macherey-Nagel) according to the manufacturer’s instructions and eluted in 30 µL.
-
bioRxiv - Microbiology 2020Quote: ... DNase digestion was performed post-column binding (Macherey-Nagel, Catalog #740963) at room temperature for 5 minutes at a final concentration of 40 ng/μL ...
-
bioRxiv - Cell Biology 2020Quote: ... DNA fragments were purified using NucleoSpin (Macherey-Nagel) and diluted to 1/100 for input and to the half for immunoprecipitated fractions ...
-
bioRxiv - Cell Biology 2021Quote: ... Fragments were gel purified (following kit instructions, Macherey-Nagel) and 30 ng of insert and 10 ng of vector ligated using 6U of T4 DNA ligase (Thermo ...
-
bioRxiv - Developmental Biology 2021Quote: ... PCR fragments were extracted from agarose gels (Macherey-Nagel, 740609.250). pBlueRabbit vector was digested with EagI and XbaI ...
-
bioRxiv - Cell Biology 2023Quote: ... The large DNA fragment was gel purified (Macherey-Nagel, 740609.50) and used as a target backbone for cloning the AviTag constructs ...
-
bioRxiv - Genomics 2023Quote: ... DNA was then precipitated after addition of an equal volume of binding buffer C4 (MACHEREY-NAGEL) and 99% ethanol overnight at 4°C or 1 h in ice then centrifuged at 800 rpm for 10 min ...
-
bioRxiv - Neuroscience 2022Quote: RNA was extracted from purified astrocyte preparations using RNA-binding columns (NucleoSpin RNA plus Macherey-Nagel #740984.250). RNA was analyzed using TapeStation ...
-
bioRxiv - Plant Biology 2020Quote: ... The fragment was purified using a NucleoSpin® kit (Macherey-Nagel, Düren, Germany) and cloned into the pENTR/SD/D-Topo vector (Invitrogen ...
-
bioRxiv - Synthetic Biology 2020Quote: ... PCR fragments were purified using a PCR Purification Kit (Macherey-Nagel, Duren, Germany), and plasmids were purified with a Plasmid Miniprep Kit (Macherey-Nagel).
-
bioRxiv - Synthetic Biology 2019Quote: ... The amplified fragment was purified with NucleoSpin Gel and PCR clean-up (Macherey-Nagel) and ligated with T4 DNA Ligase (Thermo-Fisher) ...
-
bioRxiv - Synthetic Biology 2019Quote: The amplified DNA fragments were then gel purified with NucleoSpin Extract II kit (Macherey-Nagel) and quantified ...
-
bioRxiv - Microbiology 2021Quote: ... DNA fragments were purified by using NucleoSpin Gel and PCR Clean-up kits (Macherey-Nagel). Oligos were synthesized by TAG Copenhagen A/S and DNA sequences were sequenced at Eurofins Genomics ...
-
bioRxiv - Molecular Biology 2022Quote: ... Amplified fragments were purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Germany) and DNA concentrations were measured by NanoDrop.
-
bioRxiv - Cancer Biology 2023Quote: ... then the larger linear fragment pBA439_linear was recycled and purified by columns (Macherey-Nagel, 740609.50). The ssDNA_UMI20 was assembled with pBA439_linear to construct a pBA439_UMI20 library by the NEBuilder HiFi DNA assembly (NEB ...
-
bioRxiv - Physiology 2021Quote: ... mRNA specimens were isolated from the cells grown on collagen-gel in 24-well dishes using a NucleoSpin RNAplus RNA isolation kit (Macherey-Nagel, Dueren, Germany) and converted to cDNA with SuperScript IV VILO Master Mix (Invitrogen ...
-
bioRxiv - Cancer Biology 2021Quote: ... cDNA fragments were purified with NucleoSpin gel and PCR clean-up columns (Macherey-Nagel, Düren, Germany) and were Sanger- sequenced using their corresponding primers by VBC Biotech (Vienna ...
-
bioRxiv - Microbiology 2022Quote: ... PCR fragments were purified with the Gel and PCR cleanup kit (Cat. n° 740609.250, Macherey-Nagel) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... The resulting fragments were gel purified with NucleoSpin Gel and PCR Clean-Up Kit (Macherey-Nagel). The 3×FLAG-tag (DYKDHDGDYKDHDIDYKDDDDK ...
-
bioRxiv - Synthetic Biology 2023Quote: ... All PCR fragments were gel-purified using NucleoSpin Gel and PCR Clean-up Kit (MACHEREY-NAGEL) by following manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: ... PCR fragments were cleaned up using NucleoSpin gel and PCR clean-up kit (740609.50, MACHEREY-NAGEL) according to the users’ manual and subjected to next generation sequencing (NGS) ...
-
bioRxiv - Microbiology 2024Quote: ... The linear fragments were purified on agarose gels (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel). Following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: RNA from purified astrocytes or brain cell suspension after enzymatic digestion was extracted using RNA-binding columns (NucleoSpin RNA plus Macherey-Nagel #740984.250), genomic DNA was eliminated and cDNA was synthetised (Thermo Fisher Scientific #K1672) ...
-
bioRxiv - Biochemistry 2021Quote: ... purified PCR fragment (purified using Nucleospin® Gel and PCR Clean-up, MACHEREY-NAGEL GmbH & Co. KG), and H2O to acquired final volume ...
-
bioRxiv - Microbiology 2021Quote: ... PCR fragments were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, ref. 740609.50). Plasmids were purified using the NZYMiniprep kit (NZYTech ...
-
bioRxiv - Cell Biology 2021Quote: ... PCR fragments and plasmids were respectively purified with NucleoSpin® Gel and PCR cleanup (Macherey-nagel # 740609) and NucleoSpin® Plasmid (Macherey-Nagel #740499) ...
-
bioRxiv - Biochemistry 2022Quote: ... 4°C) and fragments from the supernatant purified with a Nucleospin PCR clean-up kit (Macherey-Nagel). Illumina sequencing libraries were prepared using the Hyperprep kit (KAPA ...
-
bioRxiv - Molecular Biology 2020Quote: ... 4 °C) and fragments from the supernatant purified with a Nucleospin PCR clean-up kit (Macherey-Nagel). Experimental success was evaluated by capillary electrophoresis (Agilent ...
-
bioRxiv - Molecular Biology 2021Quote: ... The DNA fragments were then purified with a NucleoSpin Gel and PCR Clean-Up Kit (Macherey-Nagel). The integrity of the purified DNA fragments was confirmed by 2% (w/v ...
-
bioRxiv - Molecular Biology 2022Quote: ... PCR fragments were further subjected to PCR clean-up using PCR clean-up kit (Macherey-Nagel™). Electrocompetent cells were produced according to the Invitrogen Pichia Expression Kit (Catalogue no ...
-
bioRxiv - Microbiology 2023Quote: ... DNA fragments used for cloning reactions were purified by using the Nucleospin gel extraction kit (Macherey-Nagel) according to the manufacturer’s specifications ...
-
bioRxiv - Molecular Biology 2023Quote: ... 4 °C) and fragments from the supernatant were purified by PCR clean-up spin column (Macherey-Nagel).
-
bioRxiv - Plant Biology 2023Quote: ... fragments of interest were purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel), and sequenced with Eurofins SupremeRun reactions.
-
bioRxiv - Synthetic Biology 2021Quote: ... DNA fragments were purified with a NucleoSpin™ Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Restriction enzymes and T4 DNA ligase were obtained from Thermo Fisher Scientific and were used according to the supplier’s specifications ...
-
bioRxiv - Microbiology 2019Quote: ... The 8kb PCR fragment was gel extracted using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and eluted in P3 Buffer (Lonza ...
-
bioRxiv - Microbiology 2023Quote: ... DNA fragments used in cloning reactions were purified using the NucleoSpin gel and PCR cleanup kit (Macherey-Nagel) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2023Quote: ... the fragments were purified using a PCR/Gel purification kit (NucleoSpin Gel and PCR Clean□up, Macherey-Nagel) and sequenced using the smt0-seq-Rv oligonucleotide ...
-
bioRxiv - Microbiology 2024Quote: ... DNA fragments and PCR products were purified using the Nucleospin Gel and PCR clean up kit (Macherey-Nagel).
-
bioRxiv - Molecular Biology 2021Quote: ... Fragments of 300–2000 bp were excised from the gel and isolated with a gel-extraction kit (Macherey-Nagel, NucleoSpin Gel and PCR Clean-up ...
-
bioRxiv - Biochemistry 2020Quote: ... After gel extraction of the DNA fragments with the PCR Clean-up Gel Extraction Kit (Macherey-Nagel, Düren, Germany), the DNA fragments and the vector DNA were digested by the same restriction enzymes (Supplemental Table S4 ...
-
bioRxiv - Cell Biology 2021Quote: ... The amplified rDNA gene fragments were purified using nucleobond AX 100 columns for Miniprep System (Macherey-Nagel, ref 740521.100). The rDNA sequences were then labeled with Digoxigenin-11-dUTP by nick translation according to the manufacturer’s protocol (Roche ...
-
bioRxiv - Molecular Biology 2021Quote: ... DNA fragments were purified from agarose gels using the NucleoSpin Gel and PCR Clean-up kit from Macherey-Nagel. Genomic DNA extractions from Amycolatopsis and E ...
-
bioRxiv - Molecular Biology 2022Quote: ... All PCR fragments for cloning and plasmids were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and GenElute™ Plasmid Miniprep Kit (Merck) ...
-
bioRxiv - Immunology 2023Quote: ... The excised DNA fragments were further refined using nucleospin gel and by using PCR clean-up kit (Macherey-Nagel) and quantified for determining the purity and concentration ...
-
bioRxiv - Molecular Biology 2023Quote: ... The linearised fragment was then excised and purified using the NucleoSpin Gel and PCR Clean up Kit (Macherey-Nagel). 400nM of the annealed oligos were ligated with lng of digested crRNA backbone using T4 ligase (Promega ...
-
bioRxiv - Genetics 2020Quote: ... PCR reactions were separated on agarose gels and PCR fragments isolated using a Nucleo-Spin Gel and PCR Clean-up kit (Macherey-Nagel). Sanger sequencing of PMEL and KIT ...
-
bioRxiv - Immunology 2020Quote: ... and eGFP were amplified with the following primers and appropriate sized fragments were isolated from agarose gels with the PCR clean-up Gel extraction (Macherey-Nagel). PCR fragments were mixed and a consecutive PCR was done with the CD200R-F and GFP-R primers before digestion with PmlI and NotI and ligation into pMX ...
-
bioRxiv - Genomics 2024Quote: ... Cross-linking was reversed by overnight incubation at 65°C and DNA fragments were purified using NucleoSpin Gel and PCR Clean-up columns (Macherey-Nagel).
-
bioRxiv - Cell Biology 2021Quote: ... Eluates were digested with proteinase K and RNAse A and DNA fragments were purified using a PCR purification kit (Macherey-Nagel, #740609).