Labshake search
Citations for Tonbo Biosciences :
1 - 45 of 45 citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... were stimulated in a 96-well round-bottom plate with 5 µg/mL ancestral and variant SARS-CoV-2 RBD peptides and 1 µg/mL anti-CD28 (40-0281-M001; Tonbo Biosciences, San Diego, California, USA) at 37°C and 5% CO2 ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were subjected to Fc receptor blocking using Fc Shield (Tonbo Biosciences) for 10 minutes ...
-
bioRxiv - Microbiology 2023Quote: ... cells were stained with a Fc receptor block (Fc Shield, Tonbo Biosciences, Cat. No. 70-0161), a rabbit antibody against the MHV68 ORF4 protein (vRCA ...
-
bioRxiv - Cancer Biology 2023Quote: ... a Fc receptor blocker (Tonbo Biosciences 70-0161-M001 ...
-
bioRxiv - Biochemistry 2023Quote: ... samples were resuspended in 300 μL of 0.5% Triton X-100 (200 μL) + 2 μL of Fc Shield (anti-mouse CD16/CD32, Tonbo Biosciences, 70-0161-U500) + 100 μL of 5% BSA/PBS and incubated overnight at 4°C in the dark ...
-
bioRxiv - Immunology 2021Quote: ... splenocytes were immediately blocked for Fc (Tonbo Bioscience) and stained for B8R tetramer (AF488 ...
-
bioRxiv - Immunology 2022Quote: Single cell suspensions were stained at 4°C in the dark with fixable live/dead dye diluted in PBS for 10 minutes followed by Fc-receptor blockade with Fc Shield (Tonbo Biosciences) for 15 minutes ...
-
bioRxiv - Immunology 2022Quote: ... and anti-CD16/32 Fc block (2.4G2, Tonbo Biosciences). Measurement of mitochondrial content was performed using MitoTracker Green (Molecular Probes ...
-
bioRxiv - Immunology 2021Quote: ... Splenocytes were then subjected to FC block (Tonbo Bioscience) and stained with anti-CD3 (FITC ...
-
bioRxiv - Immunology 2024Quote: ... Fc block (clone 93, BioLegend or clone 2.4G2, Tonbo Biosciences), and recombinant mouse TGF-β (5231 ...
-
bioRxiv - Neuroscience 2024Quote: ... Samples were resuspended in FACS buffer (2% FBS, 2mM EDTA, PBS) and then incubated for 10min at 4C in Fc Block (Tonbo Biosciences cat # 70-0161-U500, 1:2000) and washed with FACS buffer ...
-
bioRxiv - Bioengineering 2020Quote: ... cells were blocked with Fc receptor antibody (clone: 2.4G2, Tonbo Biosciences) and then stained with fluorochrome conjugated antibodies ...
-
bioRxiv - Cancer Biology 2020Quote: ... samples were blocked in αCD16/32 mouse Fc block (Tonbo Biosciences #70-0161) and stained for extracellular proteins using an antibody master mix made in buffer ...
-
bioRxiv - Immunology 2020Quote: ... followed by incubation with 1 μg Fc Block (anti-CD16/32, Tonbo Biosciences) for 15 minutes at 4°C ...
-
bioRxiv - Immunology 2021Quote: Staining panels for mouse included anti-Fc (anti-CD16/CD32) (Tonbo Biosciences, 2.4G2), anti-CD11b (Tonbo Biosciences ...
-
Accumulation of TCR signaling from self-antigens in naive CD8 T cells mitigates early responsivenessbioRxiv - Immunology 2023Quote: ... prior to Fc-block incubation (anti-mouse CD16/CD32, clone 2.4G2, Tonbo Biosciences). CD8+ T cells were purified by negative selection using biotinylated antibodies and magnetic beads ...
-
bioRxiv - Immunology 2023Quote: ... Cell staining was conducted in the presence of Fc Block (2.4G2, Tonbo Biosciences) at 4°C for 30 minutes for all surface markers except CXCR5-biotin which was stained at room temperature for 45 minutes ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated in Fc block (1:100, Tonbo Bioscience, 70-0161-U500) followed by indicated antibodies and fixed with 1% PFA in flow cytometry buffer ...
-
bioRxiv - Cancer Biology 2023Quote: ... a Fc receptor blocker (Tonbo Biosciences 70-0161-M001, Tonbo Biosciences, San Diego, CA) was added for 10 minutes at 4°C to prevent non-specific binding of antibodies ...
-
bioRxiv - Immunology 2021Quote: ... Anti-mouse CD16/32 antibody (2.4G2) used for Fc receptor blocking was obtained from Tonbo Biosciences. Unconjugated mouse and rat IgG antibodies were purchase from Jackson ImmunoResearch (West Grove ...
-
bioRxiv - Immunology 2024Quote: DAPI (#11034-56, Nacalai Tescue) and Fc block (clone 93, BioLegend or clone 2.4G2, Tonbo Biosciences) were added to the cells just before staining with specific antibodies against cell surface molecules ...
-
bioRxiv - Immunology 2023Quote: Cells were then incubated with Fc block (anti-CD16/32, 10 µg/ml, Tonbo Biosciences, San Diego, CA) for 15-minute on ice ...
-
bioRxiv - Immunology 2023Quote: ... and resuspended in staining buffer containing Fc block (anti-CD16/32, 10 µg/ml, Tonbo Biosciences, San Diego, CA) for 25-minute on ice ...
-
bioRxiv - Neuroscience 2024Quote: Cells were then incubated for 10min at 4C in Fc Block (Tonbo Biosciences cat # 70-0161-U500, 1:2000) then washed with FACS buffer ...
-
bioRxiv - Immunology 2024Quote: ... Anti-mouse CD16/CD32 Fc Block antibody purchased at a concentration of 2mg/mL (Tonbo Biosciences 40-0161-M001) was added at 0.5 uL per sample (in 100uL total volume per sample of block antibody diluted in PBS ...
-
bioRxiv - Microbiology 2021Quote: ... samples were washed with PBS pH7.2 (Fisher Cat#20012027) and incubated with Fc Block (Tonbo Biosciences Cat# 70-0161-U500) for 5 minutes on ice before viability staining (BD Biosciences Cat# 564406) ...
-
bioRxiv - Developmental Biology 2023Quote: Lungs were plated at 1 × 106 cells per well and stained with Fc block (CD16/32, 1:100, Tonbo Biosciences) for 30 min on ice ...
-
bioRxiv - Developmental Biology 2022Quote: Lung cells were plated at 1×106 cells per well and stained with Fc block (CD16/32, 1:100, Tonbo Biosciences) for 30 min on ice ...
-
bioRxiv - Immunology 2021Quote: ... 0.025% NaN3) and Fcγ receptors were blocked with 1/200 diluted anti-mouse CD16/32 antibodies (Fc block) (Tonbo Biosciences, San Diego, CA, USA) for 10 min on ice ...
-
bioRxiv - Immunology 2022Quote: ... Cells were stained using a dilution of 1:400 of anti-mouse CD16/CD32 (Fc Shield, #70-0161-U100; TONBO biosciences, San Diego, USA) in cRPMI for 15 minutes ...
-
bioRxiv - Microbiology 2021Quote: ... and IL-2-APC (Tonbo bioscience, 20-7021) on ice for 30 min ...
-
bioRxiv - Immunology 2020Quote: ... 2 μl of anti-human CD19-PECy7 antibody (clone HIB19, TONBO biosciences), and 3 μl of anti-human TCRab-AF488 antibody (clone IP26 ...
-
bioRxiv - Immunology 2020Quote: ... 2 μl of anti-human CD19-PECy7 antibody (clone HIB19, TONBO biosciences), and 3 μl of anti-human TCRab-AF488 antibody (clone IP26 ...
-
bioRxiv - Immunology 2020Quote: Mice were intravenously (i.v.) injected with 2 µg of α-CD45 (Clone: 30-F11) (Tonbo Biosciences) diluted in 300 µL of sterile PBS five minutes before sacrifice ...
-
bioRxiv - Immunology 2023Quote: ... mice were intravenously injected by retro-orbital injection with 2 μg α-CD45-FITC (30-F11, Tonbo Biosciences) antibody to detect remaining circulating cells in lung samples (52) ...
-
bioRxiv - Immunology 2023Quote: Freshly thawed UCBMCs (1-2×106 cells) were stained with Ghost Violet 540 (Tonbo Biosciences, San Diego, CA) for 30 min at 4C in the dark before being incubated with Fc blocker (Human TruStain FcX ...
-
bioRxiv - Immunology 2023Quote: Freshly thawed villous leukocytes (1-2×106 cells) were stained with Ghost Violet 540 (Tonbo Biosciences, San Diego, CA) for 30 min at 4C in the dark before being incubated with Fc blocker (Human TruStain FcX ...
-
bioRxiv - Bioengineering 2023Quote: ... A cell pellet formed and was resuspended in 2 mL of 1× lysis buffer (Tonbo Biosciences, San Diego, CA) and incubated at room temperature for 15 min ...
-
bioRxiv - Microbiology 2019Quote: ... bulk CD4+ T cells were immediately plated on anti-CD3 coated plates [coated for 2 hours at 37°C with 20µg/mL anti-CD3 (UCHT1, Tonbo Biosciences)] in the presence of 5µg/mL soluble anti-CD28 (CD28.2 ...
-
bioRxiv - Immunology 2021Quote: ... After the incubation a cocktail of fluorescently-labeled surface antibodies (2 µL of each: Ghost Dye Violet 510 Viability Dye, Tonbo Biosciences 13-0870-T100 ...
-
bioRxiv - Cell Biology 2022Quote: ... Samples were centrifuged and resuspended in FACS buffer (PBS supplemented with 2% FBS) and stained with Ghost Dye Violet 510 (1:1000, Tonbo Biosciences) on ice for 10 min for discrimination of viable and non-viable cells ...
-
bioRxiv - Microbiology 2024Quote: ... bulk CD4+ T cells were immediately plated on anti-CD3–coated plates coated for 2 hours at 37°C with anti-CD3 (20 mg/ml) (UCHT1; Tonbo Biosciences)] in the presence of soluble anti-CD28 (5 mg/ml ...
-
bioRxiv - Immunology 2019Quote: ... CD4+ T cells were stimulated with plate-bound α-CD3 and soluble α-CD28 antibodies (both at 2 μg/ml; Tonbo Biosciences) for 72 hours ...
-
bioRxiv - Immunology 2022Quote: Single-cell suspensions were washed with FACS buffer (1X PBS/2% FBS/2mM EDTA/0.05% sodium azide) and stained with ghost dye red 780 viability dye (Tonbo Biosciences 13-0865-T100) and antibodies against TCRβ (H57-597 ...
-
bioRxiv - Immunology 2021Quote: ... and SARS-CoV-2 naive DPB1*04:01 positive donors’ PBMCs were stained with 1 µL Ghost Dye Violet 510 Viability Dye (Tonbo Biosciences 13- 0870-T100) and 1 µL of DPB1*04:01-S167-180-tetramer ...