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Citations for Tonbo Biosciences :
1 - 22 of 22 citations for 7 benzyl 2 7 diazaspiro 4.5 decane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... and 10ng/mL IL-7 (Tonbo Biosciences, 218079U002). Stimulated PBMCs were electroporated using the Neon transfection system (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... incubated with 7-AAD (Tonbo Bioscience #13-6993-T500) for 20 minutes at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were suspended in 0.5μg/ml 7-AAD (13-6993-T500, Tonbo Biosciences) in 1mL staining solution [900μL H2O + 100μL NaCitrate (380mM)] for 15 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: BMDCs and MLN cells were stained with the following Abs to identify DCs: anti-CD11c-PE/Cyanine 7 (clone; N418, TONBO Biosciences) and anti-I-A/I-E-PerCP (clone ...
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bioRxiv - Microbiology 2021Quote: ... and IL-2-APC (Tonbo bioscience, 20-7021) on ice for 30 min ...
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bioRxiv - Immunology 2020Quote: ... 2 μl of anti-human CD19-PECy7 antibody (clone HIB19, TONBO biosciences), and 3 μl of anti-human TCRab-AF488 antibody (clone IP26 ...
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bioRxiv - Immunology 2020Quote: ... 2 μl of anti-human CD19-PECy7 antibody (clone HIB19, TONBO biosciences), and 3 μl of anti-human TCRab-AF488 antibody (clone IP26 ...
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bioRxiv - Immunology 2020Quote: Mice were intravenously (i.v.) injected with 2 µg of α-CD45 (Clone: 30-F11) (Tonbo Biosciences) diluted in 300 µL of sterile PBS five minutes before sacrifice ...
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bioRxiv - Immunology 2023Quote: ... mice were intravenously injected by retro-orbital injection with 2 μg α-CD45-FITC (30-F11, Tonbo Biosciences) antibody to detect remaining circulating cells in lung samples (52) ...
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bioRxiv - Immunology 2023Quote: Freshly thawed UCBMCs (1-2×106 cells) were stained with Ghost Violet 540 (Tonbo Biosciences, San Diego, CA) for 30 min at 4C in the dark before being incubated with Fc blocker (Human TruStain FcX ...
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bioRxiv - Immunology 2023Quote: Freshly thawed villous leukocytes (1-2×106 cells) were stained with Ghost Violet 540 (Tonbo Biosciences, San Diego, CA) for 30 min at 4C in the dark before being incubated with Fc blocker (Human TruStain FcX ...
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bioRxiv - Bioengineering 2023Quote: ... A cell pellet formed and was resuspended in 2 mL of 1× lysis buffer (Tonbo Biosciences, San Diego, CA) and incubated at room temperature for 15 min ...
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bioRxiv - Microbiology 2019Quote: ... bulk CD4+ T cells were immediately plated on anti-CD3 coated plates [coated for 2 hours at 37°C with 20µg/mL anti-CD3 (UCHT1, Tonbo Biosciences)] in the presence of 5µg/mL soluble anti-CD28 (CD28.2 ...
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bioRxiv - Immunology 2021Quote: ... After the incubation a cocktail of fluorescently-labeled surface antibodies (2 µL of each: Ghost Dye Violet 510 Viability Dye, Tonbo Biosciences 13-0870-T100 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were centrifuged and resuspended in FACS buffer (PBS supplemented with 2% FBS) and stained with Ghost Dye Violet 510 (1:1000, Tonbo Biosciences) on ice for 10 min for discrimination of viable and non-viable cells ...
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bioRxiv - Microbiology 2024Quote: ... bulk CD4+ T cells were immediately plated on anti-CD3–coated plates coated for 2 hours at 37°C with anti-CD3 (20 mg/ml) (UCHT1; Tonbo Biosciences)] in the presence of soluble anti-CD28 (5 mg/ml ...
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bioRxiv - Immunology 2019Quote: ... CD4+ T cells were stimulated with plate-bound α-CD3 and soluble α-CD28 antibodies (both at 2 μg/ml; Tonbo Biosciences) for 72 hours ...
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bioRxiv - Immunology 2022Quote: Single-cell suspensions were washed with FACS buffer (1X PBS/2% FBS/2mM EDTA/0.05% sodium azide) and stained with ghost dye red 780 viability dye (Tonbo Biosciences 13-0865-T100) and antibodies against TCRβ (H57-597 ...
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bioRxiv - Biochemistry 2023Quote: ... samples were resuspended in 300 μL of 0.5% Triton X-100 (200 μL) + 2 μL of Fc Shield (anti-mouse CD16/CD32, Tonbo Biosciences, 70-0161-U500) + 100 μL of 5% BSA/PBS and incubated overnight at 4°C in the dark ...
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bioRxiv - Immunology 2021Quote: ... and SARS-CoV-2 naive DPB1*04:01 positive donors’ PBMCs were stained with 1 µL Ghost Dye Violet 510 Viability Dye (Tonbo Biosciences 13- 0870-T100) and 1 µL of DPB1*04:01-S167-180-tetramer ...
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bioRxiv - Immunology 2023Quote: ... were stimulated in a 96-well round-bottom plate with 5 µg/mL ancestral and variant SARS-CoV-2 RBD peptides and 1 µg/mL anti-CD28 (40-0281-M001; Tonbo Biosciences, San Diego, California, USA) at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were resuspended in FACS buffer (2% FBS, 2mM EDTA, PBS) and then incubated for 10min at 4C in Fc Block (Tonbo Biosciences cat # 70-0161-U500, 1:2000) and washed with FACS buffer ...