Labshake search
Citations for Bimake :
1 - 46 of 46 citations for 7 METHYLIMIDAZO 1 2 B 1 2 4 TRIAZINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... 2 μL of 1:10 diluted cDNA was added to 10 μL of 2× SYBR® Green Supermix (Bimake, Houston, Texas, USA) and the final concentration of each primer was 250 nM in 20 μL of total volume ...
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bioRxiv - Cancer Biology 2020Quote: In vitro cell proliferation was determined using WST-8 [water soluble tetrazolium-8 or 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulphophenyl)-2H-teterazolium] assay (Bimake; Munich, Germany). For this ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM tris(2-carboxyethyl)phosphine(TCEP) 10% (v/v) glycerol with additional EDTA-free Protease Inhibitor Cocktail (Bimake), and then incubated with agitation for 20 min at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 mM NaCl and 2 mM MgCl2 supplemented with EDTA-free protease inhibitor cocktail (Bimake) by dounce homogenization ...
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bioRxiv - Immunology 2020Quote: ... The cDNA was mixed with 2 × SYBR Green qPCR Master Mix (B21202, Bimake, Houston, TX, USA), and the real-time PCR reaction was performed on the 7500 Real-Time PCR System (ABI ...
Product cited:
Bimake 2x SYBR Green qPCR master mix performs qPCR with highest sensitivity, specIFicity and...Cat# B21202, SKU# B21202-5 mL, 5 mL, $137.00 Ask -
bioRxiv - Cancer Biology 2023Quote: ... and phosphatase inhibitor mixture mixes A and B (100X) (Bimake). The lysates were cleared by centrifugation at 13,000 rpm at 4°C in a microcentrifuge for 10 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time PCR was performed in triplicate or quadruplicate using 2 x SYBR Green qPCR Master Mix (Bimake.com) with specific primers and normalized to reference genes (Table 1).
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bioRxiv - Plant Biology 2021Quote: qRT-PCR was performed with 2X SYBR Green qRT-PCR Master Mix with Rox #2 (Bimake; Houston, TX, USA), as follows per well ...
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bioRxiv - Developmental Biology 2022Quote: ... Quantitative PCR was performed by using the Agilent Mx3000P qPCR system with the 2×SYBR Green qPCR Master Mix (Bimake). Relative mRNA expression was determined by the delta cycle time with human GAPDH as the internal control in data normalization ...
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bioRxiv - Neuroscience 2020Quote: ... Real-time polymerase chain reaction was carried out in duplicate using 2× SYBR® Green Supermix (Bimake, Houston, Texas, USA), and reactions were run on a CFX Connect Real-Time PCR Detection System (BioRad Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... Nphp1 cDNA fragments between exons 2 and 11 were PCR-amplified using Universe High-Fidelity Hot Start DNA polymerase (Bimake) and two primers (F1-mNPHP1-RT and R1-mNPHP1-RT ...
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bioRxiv - Developmental Biology 2023Quote: ... Then the cDNA was subjected to quantitative RT-PCR (qRT-PCR) using the SYBR green assay with 2× SYBR green qPCR master mix (Bimake). Thermal profile was 95 °C for 5 min and 40 cycles of 95 °C for 15 sec and 60 °C for 20 sec ...
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bioRxiv - Genomics 2023Quote: ... brought the volume of RIPA to 1 mL and supplemented it with 1× PIC (Bimake, B14001). Subsequent incubation with antibodies ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Cancer Biology 2021Quote: ... seeded in 96-well plates (2×103/well, five replicates) and cell viability was measured with Cell Counting Kit-8 (CCK-8) (Bimake.com, China). The CCK-8 test was repeated every 24 hrs for three days.
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bioRxiv - Microbiology 2023Quote: ... Infected cells were collected at 2 days post infection and washed three time with PBS supplemented with protease inhibitor cocktail (Bimake, B14001). The cell pellets were resuspended in lysis buffer (Beyotime ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Cancer Biology 2021Quote: ... 1 × Protease Inhibitor Cocktail (Bimake; Cat#: B14001)) on ice for 15 min ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Developmental Biology 2024Quote: ... and 1% phosphatase inhibitor cocktail (B15001, Bimake) at 4°C for 20 minutes ...
Product cited:
Phosphatase Inhibitor Cocktail for phosphorylation protection is provided in a 2 tube formula at...Cat# B15001, SKU# B15001-1 mL A + 1mL B, 1 mL A + 1mL B, $54.00 Ask -
bioRxiv - Developmental Biology 2024Quote: ... 1% EDTA-free protease inhibitor cocktail (B14001, Bimake) and 1% phosphatase inhibitor cocktail (B15001 ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Molecular Biology 2021Quote: ... protease inhibitor cocktail (Bimake, 1 mL/ 100 mL suspension). The cell pellets were dounced to homogeneity and subsequently added with 1mM dopamine (MCE Inhibitors ...
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bioRxiv - Cancer Biology 2021Quote: ... containing proteinase inhibitor an phosphatase inhibitor (1/100; Bimake) by sonication using Branson Sonifier 150 with a duty cycle at 25% ...
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bioRxiv - Biochemistry 2022Quote: ... protease inhibitor cocktail (Bimake, 1 mL/ 100 mL suspension). The protein complex was assembled on membrane by adding 100 μM rotigotine (TargetMol ...
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bioRxiv - Biochemistry 2022Quote: Cells were lysed in RIPA buffer (Thermo) containing protease and phosphatase inhibitor cocktails (Bimake B14001 and B15001-B). Protein concentration was quantified by BCA assay (Thermo Fisher) ...
Product cited:
Phosphatase Inhibitor Cocktail for phosphorylation protection is provided in a 2 tube formula at...Cat# B15001, SKU# B15001-1 mL A + 1mL B, 1 mL A + 1mL B, $54.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... 1% Triton X-100) supplemented with Protease Inhibitor Cocktail (Bimake). Homogenates were centrifuged at 20,000 xg for 15 min at 4 °C to remove insoluble materials ...
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bioRxiv - Neuroscience 2021Quote: ... 1% Triton X-100) supplemented with Protease Inhibitor Cocktail (Bimake). Homogenates were clarified by centrifugation at 20,000x g for 15 min at 4 °C and supernatants were collected ...
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bioRxiv - Cancer Biology 2020Quote: ... 1% Triton X-100) with EDTA-free protease inhibitors (Bimake) and phosphatase inhibitors (Bimake ...
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bioRxiv - Molecular Biology 2023Quote: ... 1% Triton X-100) supplemented with 1x protease inhibitor (Bimake.com) and SuperNuclease (Sino Biological) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1% Triton X-100) supplemented with Protease Inhibitor Cocktail (Bimake). Lysates were centrifuged at 20,000x g for 15 minutes at 4°C to precipitate insoluble materials.
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bioRxiv - Bioengineering 2020Quote: ... 1% Nonidet P-40) supplemented with protease inhibitor cocktail (Bimake B14001) for 1 hour on ice ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Molecular Biology 2021Quote: ... anti-TUBULIN rabbit antibody (Bimake, Houston, TX, USA, A5105, 1:1000), and then detected with sheep anti-mouse IgG-HRP (GE Healthcare ...
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bioRxiv - Genetics 2020Quote: ... 1% Nonidet P-40) supplemented with protease inhibitor cocktail (Bimake B14001) for 1 hour on a rotator at 4°C ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Molecular Biology 2021Quote: ... 1% SDS) supplemented with protease inhibitor cocktail (Bimake, Cat. No. B14002) and phosphatase inhibitor cocktail (Bimake ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14002, SKU# B14002-1 mL x 10, 1 mL x 10, $270.00 Ask -
bioRxiv - Genetics 2020Quote: ... 1% Nonidet P-40) supplemented with protease inhibitor cocktail (Bimake B14001) for 1 hour on a rotator at 4°C ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Plant Biology 2019Quote: ... 1% (v/v) Protease Inhibitor Cocktail (Bimake, added immediately prior to use) was added to the frozen powder (2 ml per g fresh weight ...
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bioRxiv - Cancer Biology 2022Quote: ... 1% NP-40 and 5% glycerol) supplemented with protease inhibitors (Bimake, Cat# B14012) and phosphatase inhibitors (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... 1% w/v deoxycholic acid (sodium salt)) supplemented with protease inhibitors (Bimake, Cat# B14001) followed by sonication ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Molecular Biology 2022Quote: ... 1 mM MnCl2 and 100 μM TCEP supplemented with EDTA-free protease inhibitor cocktail (Bimake) by dounce homogenization ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM MnCl2 and 100 μM TCEP supplemented with EDTA-free protease inhibitor cocktail (Bimake) by dounce homogenization ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1% Triton X-100) containing a cocktail of protease and phosphatase inhibitors (Bimake, Houston, TX), sonicated (3 × 45 s in a Sonic Dismembrator apparatus ...
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bioRxiv - Genomics 2019Quote: ... Cells were washed with cold PBS and incubated in 375 µL lysis buffer (10 mM Tris pH 7.5, 10 mM NaCl, 0.2% NP40, 1× protease inhibitors (Bimake), 37.5 U SUPERase.In RNase inhibitor (Invitrogene) ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were then washed twice with cold PBS and resuspended in SDS lysis buffer (50 mM Tris– HCl, pH8.1, 10 mM EDTA, 0.5% SDS, 1×Protease inhibitor cocktail (Bimake)) ...
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bioRxiv - Genomics 2021Quote: Total proteins were extracted using the RIPA lysis buffer (Beyotime Biotechnology, Shanghai, China) containing 1×complete proteinase inhibitor (Bimake), and protein concentration was defined using BCA protein assay kit (Beyotime) ...
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bioRxiv - Genomics 2023Quote: ... The pellet was resuspended in 550 μL of ice cold ChIP lysis buffer (50 mM Tris-HCl pH 8.0, 1% SDS, 10 mM EDTA) supplemented with 0.5× PIC (Bimake, B14001) and 0.5 mM PMSF ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Genomics 2023Quote: ... Permeabilized cells were resuspended in 550 μL of ice cold ChIP lysis buffer (50 mM Tris-HCl pH 8.0, 1% SDS, 10 mM EDTA) supplemented with 0.5× PIC (Bimake, B14001) and 0.5 mM PMSF ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Immunology 2021Quote: ... Spleens and graft tissues were homogenized in SDS free RIPA buffer of pH 8.0 (10mM Tris HCL, 1mM EDTA, 140mM NaCl and 1% Triton X-100) supplemented with protease inhibitor cocktail (Bimake.com, Houston, TX) using a micro-tube homogenizer (Bel-Art ...
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bioRxiv - Microbiology 2023Quote: ... Cells were pelleted at 13,000 g × 5 min and resuspended in lysis buffer (PBS buffer supplemented with 1 × Protease Inhibitor Cocktail (Bimake, Houston, TX, USA), 1mg/ml lysozyme) ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μL of 1:10 diluted cDNA was added to 10 μL of 2x SYBR Green qPCR Master Mix (Low Rox) (Bimake, Houston, TX, USA). The final concentration of each primer was 250 nM in 20 μL of total master mix volume ...