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Citations for Bio-Rad :
1 - 22 of 22 citations for GSK3A siRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Control scrambled siRNA or TRAF6 siRNA (30 nM) were transfected using siLentFect (Bio-Rad).
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bioRxiv - Cell Biology 2019Quote: ... a total of 15 μg siRNA (5 μg from each siRNA) was transferred to a 4-mm cuvette (Bio-Rad) and 5-10×106 DCs were added in 200 μl OptiMEM and incubated for 3 min before being pulsed with an exponential decay pulse at 300 V ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 15 μg siRNA (5 μg from each siRNA) was transferred to a 4-mm cuvette (Bio-Rad) and 5-10×106 DCs were added in 200 μl OptiMEM and incubated for 3 min before being pulsed with an exponential decay pulse at 300 V ...
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bioRxiv - Immunology 2023Quote: ... A total of 15 μg siRNA (5 μg from each siRNA) was transferred to a 4-mm cuvette (Bio-Rad) and 5-10×106 DCs were added in 200 μl OptiMEM and incubated for 3 min before being pulsed with an exponential decay pulse at 300 V ...
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bioRxiv - Cell Biology 2023Quote: ... siRNAs were transfected using siLentfect (Bio-Rad Laboratories) according to the manufacturer’s instructions and cells were analyzed at least 72 h post-transfection ...
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bioRxiv - Cell Biology 2020Quote: All siRNA transfections were performed using siLentFect (Bio-Rad) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were transfected with siRNA 24 hrs later using SilentFect (Bio-Rad): 37.5 µL OptiMEM (Gibco ...
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bioRxiv - Genomics 2023Quote: ... 2 million cells were resuspended in 200μl OptiMEM and incubated for 3min with 2μg (7.5μL of a 20μM stock) siRNA in a 4mm cuvette (Bio-Rad) before being pulsed at 300V ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmid transfections and siRNA mediated knock downs were done along with cell seeding using Transfectin reagent (BioRad) and Dharmafect reagent (Horizon discovery ...
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bioRxiv - Cancer Biology 2023Quote: Plasmid DNA and siRNA oligonucleotides were transfected into U373 cells using electroporation with a GenePulser Xcell (Bio-Rad Laboratories). Electroporation was performed according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... F43E2.6 siRNA and miR-1 Ct values were assayed using a CF96 Real-Time PCR Detection System (Bio-Rad). Ct values were averaged across three technical replicates for each of 3 biological replicates.
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bioRxiv - Molecular Biology 2020Quote: ... approximately 250.000 cells were plated in a 6-well dish and transfected with a final concentration of 22.5 nM siRNA using siLentFect Lipid Reagent (Bio-Rad) accordingly to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: siRNAs-treated HeLa cells and Control KO and Cavin1 KO cells were directly lysed in 1x Laemmli (Bio-Rad Laboratories) with Benzonase nuclease (#70664 Millipore ...
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bioRxiv - Bioengineering 2024Quote: ... The modified siRNA was then separated from free Azido-PEG3-Maleimide by SEC using an ENrich SEC 70 column (BioRad) on a BioRad NGC Quest 10 Plus ...
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bioRxiv - Genomics 2020Quote: ... were seeded per well in 6-well plates (2 ml per well). 24 hrs later cells were transfected with siRNA (Supp. Table 9) using SilentFect (Bio-Rad) in biological triplicates ...
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bioRxiv - Developmental Biology 2022Quote: ... The suspended eggs and siRNA (5 μg/μl stock solution, in MilliQ) were transferred to a 4 mm cuvette (Bio-Rad) with a final volume of 200 μl and were gently mixed ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative reverse transcriptase PCR (qRT-PCR) was performed for siRNA-mediated Brca1 and Brca2 depletion on qPCR CFX 96 Thermocycler (Bio-Rad) using gene-specific primers (Table EV 3) ...
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bioRxiv - Cell Biology 2021Quote: ... Selected siRNAs were spotted onto a gridded MatTek dish (P35G-2-14-C-GRID) using a contact spotter (ChipWriter Pro-Bio-Rad Laboratories) resulting in a layout of 4 × 8 spots 40 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were allowed to adhere on plate/coverslips for 24 hours and then transfected with 20 nm of siRNA using siLentFect™ lipid reagent (Bio-Rad) for 24 hours.
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bioRxiv - Cell Biology 2023Quote: 5 μg DNA/106 cells or 100 nM siRNA /106 cells were transfected by electroporation (200 mV, 950 μF and 480 Ω; Bio-Rad). Expression was measured 24-48 h post-transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... Dharmacon, Cambridge, UK) or siRNAs targeting MYC (D-003282-14 & D-003282-35, Dharmacon) using siLentFect transfection reagent (1703362, Biorad, Hercules, CA, USA) (see Table S4 for siRNA sequences) ...
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bioRxiv - Microbiology 2024Quote: ... 10 μg of total protein for both NT and Rab siRNA-transfected samples were resolved in 4-20% gradient SDS-PAGE (Mini-PROTEAN Gel, Cat. 4561094, Bio-Rad Laboratories, Hercules, CA) and subsequently transferred to a PVDF membrane for immunoblotting (Immun-Blot PVDF Membrane ...