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Citations for Bio-Rad :
401 - 450 of 1595 citations for Erythromycin 90 95% Erythromycin A N N Dimethyl 13C2 ~90% 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... 6µg of Round or Rod-shaped CCMVTT-VLPs were mixed with 2x mercaptoethanol and heated at 95°C for 3 minutes and then loaded into Any kD Mini-PROTEAN TGX precast protein gels (BIO-RAD). Gel was run for 35min at 180V ...
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bioRxiv - Microbiology 2021Quote: ... boiled for 5 min at 95 °C and separated by discontinuous sodium dodecyl sulfate (SDS) 4-15% polyacrylamide gel electrophoresis (SDS-PAGE precast gels, Bio-Rad). The SDS-PAGE gels were stained in Coomassie Blue solution (Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... and 40 cycles of PCR (5 s at 95°C followed by 20 s at 60°C) in a CFX Opus 96 Real-Time PCR System (Bio-Rad). Results were analyzed by the Comparative CT Method (ΔΔCt Method) ...
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bioRxiv - Biochemistry 2022Quote: ... was measured over temperatures ranging from 10 °C to 95 °C using a CFX Connect Real-Time PCR Detection System (Bio-Rad). Melting temperature (Tm ...
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bioRxiv - Biophysics 2022Quote: ... After boiling the beads at 95°C for 5 min in 2X Laemmli sample buffer with 5% β-mercaptoethanol (Bio-Rad), the immunoprecipitated protein was resolved on 10% SDS-PAGE gels and then transferred onto 0.45 μm PVDF membrane (GE ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were heated in reducing Laemmli sample buffer at 95°C and resolved in SDS-PAGE protein gel and transferred to PVDF membrane (Bio-Rad). Membranes were blocked using 5% BSA (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Samples were boiled for 5 min at 95°C before separation on a gradient 4 to 20% sodium dodecyl sulfate (SDS)-polyacrylamide gel (Bio-Rad). Samples were then transferred to nitrocellulose membrane using standard methods for semidry transfer (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... samples were incubated at 95°C for 5 or 10 min and then resolved on TGX Stain-free gel (Bio-Rad). The proteins were transferred onto 0.2 μm nitrocellulose membranes using Trans-Blot Turbo Transfer (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... samples were incubated at 95°C for 5 or 10 min and then resolved on TGX Stain-free gel (Bio-Rad). The proteins were transferred onto 0.2 µm nitrocellulose membranes using Trans-Blot Turbo Transfer (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were heated at 95 °C for 5 min and loaded on 12% Mini-PROTEAN TGX precast protein gel (#4561043, Bio-Rad). The gel was run using 1x Tris/Glycine running buffer at 100 V for 90 min ...
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bioRxiv - Physiology 2022Quote: ... Lysates were diluted with 4X Laemmli buffer containing beta-mercaptoethanol and boiled for 10 minutes at 95°C and subjected to SDS-polyacrylamide gel electrophoresis 4-20% (Criterion TGX Stain-Free Protein Gel, Bio-Rad) gels ...
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bioRxiv - Microbiology 2022Quote: ... Samples were heated 10 min at 95°C before loading 10 µl aliquots onto precast mini- PROTEAN TGX gels (Bio-Rad). The polyacrylamide concentration was 7.5% for GFP-DamX constructs ...
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bioRxiv - Microbiology 2022Quote: ... Samples were heated for 10 min at 95°C before loading 10 µl onto a 10% polyacrylamide precast mini-PROTEAN TGX gel (Bio-Rad). After electrophoresis the gel was washed twice with distilled water ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were heated at 95°C for 10 min and loaded into a 4-15% gradient Tris-glycine gel (Biorad 4561084) immersed in Tris-glycine-SDS running buffer (Biorad 1610772) ...
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bioRxiv - Biochemistry 2022Quote: ... with a three-step protocol (95°C 15 sec, 60°C 30 sec, 68°C 60 sec) and iTaq Universal SYBR Green Supermix (Bio-Rad). Primer sequences are provided in Table 2 ...
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β-catenin programs a tissue-specific epigenetic vulnerability in aggressive adrenocortical carcinomabioRxiv - Cancer Biology 2022Quote: Samples were prepared by boiling at 95°C for 6 minutes in lysis buffer supplemented with 4x Laemmli Sample Buffer (Bio-Rad), with 355 mM 2-mercaptoethanol freshly added according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... was added to each well and the mixture was heated from 25 to 95°C in 1°C steps of 1 min each in a CFX96 Touch™ Real-Time PCR Detection System (BioRad). Excitation and emission filters of 492 and 516 nm respectively ...
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bioRxiv - Microbiology 2023Quote: ... boiled for 5 minutes at 95°C and loaded onto Mini-Protean TGX Stain Free Gels 4-20% (Bio-Rad, P4568096). The gels were transferred onto 0.45 μm nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 20 μg protein was denatured at 95 °C for 5 min and loaded on a 4-20% precast polyacrylamide gel (BioRad, 4568094) for gel electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were mixed with an equal volume of 2X Laemmli sample buffer and incubated for 5 min at 95°C prior to separation by SDS PAGE on Criterion TGX stain-free 4-15% gradient gels (Bio-Rad). Following SDS-PAGE ...
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bioRxiv - Neuroscience 2023Quote: ... with a melt-curve from 65 to 95 °C (in 0.5 °C increments per 5 s) using CFX384 Touch Real-Time PCR Detection System (Bio-Rad, 1845384). Quantification of mRNA was determined using the comparative cycles to threshold CT (ΔΔCT ...
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bioRxiv - Bioengineering 2023Quote: ... The mixture was then heated to 95 °C for 5 min and loaded into pre-cast polyacrylamide gels (Mini-PROTEAN TGX, Bio-Rad) for electrophoresis in TGS buffer (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... 20 μg of proteins of each sample or both input and eluates from the IPs were heated in Laemmli loading buffer at 95°C for 5 min and loaded on a SDS-polyacrylamide electrophoresis gel (Bio-Rad 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were boiled for 8–10 min at 95°C and loaded onto precast 12% Bis-Tris gels (Bio-Rad) with MES SDS running buffer (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: ... Oocyte and embryo samples were boiled at 95 °C for 5 min and separated by SDS-PAGE using Any kD precast Polyacrylamide Gels (Bio-Rad). Blotting was performed onto a nitrocellulose membrane (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... and 0.1 vol of DTT 1M was added and the mixture heated at 95°C for 5 min before loading onto Any kD Mini-PROTEAN TGX Stain-Free protein gel (4568124, Bio-Rad) and migration in 1x Tris-Glycine-SDS buffer at 200V ...
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bioRxiv - Microbiology 2023Quote: ... Samples (24 µg) were denatured by heating 5 minutes at 95°C and separated by Stain-Free SDS-PAGE gel (4568096, Bio-Rad). The gel was activated using one-minute setting (Bio-Rad Imager ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then boiled at 95°C for 5 minutes and 20 μL of the samples were loaded into a pre-made polyacrylamide gel (Bio-Rad) immersed in 1x Tris-Glycine-SDS Buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were boiled at 95 ℃ for 10 minutes and resolved by SDS-PAGE using 4-20 % or 10 % precast gels (BioRad, 4561093), depending on the molecular weight of the desired protein ...
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bioRxiv - Immunology 2023Quote: ... Samples normalized to equal amounts were denatured in Laemmli’s buffer at 95°C for 10 min and resolved by SDS-PAGE using Criterion TM TGX TM Midi protein gels (Bio-Rad). Resolved proteins were transferred onto 0.2μM PVDF membrane by the Criterion TM Blotter (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... About 15 μg of protein samples from lysates were boiled at 95°C for 5 min in Laemmli sample buffer (Bio-Rad) and resolved in 4-15% gradient SDS-PAGE gels (Mini-Protean TGX ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were denatured by 5 min incubation at 95°C before running them on a TGX 4–12% or 10% gel (Bio-Rad) and transferred onto nitrocellulose membrane for hybridization with specific antibodies.
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bioRxiv - Cell Biology 2023Quote: ... Proteins samples were denatured in SDS-sample buffer for 5 min at 95°C and loaded onto Mini-Protean TGXTM gels (BioRad Laboratories) in equal amounts and subjected to electrophoresis ...
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bioRxiv - Microbiology 2024Quote: ... Samples were boiled for 10 minutes at 95°C before separation on a gradient of 4-20% SDS-PAGE gels (Bio-Rad). Samples were then transferred to nitrocellulose membrane using standard methods for either semi-dry or wet transfer (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... Supernatants were mixed with reducing SDS sample buffer and incubated for 5 min at 95°C prior to fractionation by SDS-PAGE analysis on 4–15 % Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). Western Blot analysis was performed as described previously (15).
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bioRxiv - Molecular Biology 2023Quote: ... The proteins were eluted by heating the beads (95°C for 5 min.) in 70 μL of BME 4X Laemmli buffer (Biorad #1610747). Prior to loading on a 7.5% TGX SDS-PAGE gel (Biorad #5671024) ...
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bioRxiv - Microbiology 2024Quote: ... 20µg of protein samples were heated in 1X Laemmli buffer at 95°C for 5 min and loaded on pre-cast 4-20% SDS-polyacrylamide electrophoresis gel (Bio-Rad). For the RIP experiment ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were denatured at 95°C for 10 min and run on 4-20% Criterion TGX Precast Midi Protein gels (Bio-Rad). Resolved protein was transferred to nitrocellulose using Trans-Blot turbo transfer packs ...
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bioRxiv - Cancer Biology 2024Quote: ... 40 cycles of 95 °C for 5 s and 60 °C for 10 s using a CFX Connect Real-Time PCR System (Bio-Rad). The relative expression levels of mRNA were calculated using the 2−ΔΔCt method.
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bioRxiv - Biochemistry 2024Quote: Samples were incubated at 95°C for 10 minutes and loaded onto SDS-polyacrylamide gels (Mini-Protean TGX gels 4-20%, Bio-Rad). Following gel electrophoresis ...
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bioRxiv - Molecular Biology 2023Quote: ... containing 2-Mercaptoethanol at 95°C for 10 min and separated by electrophoresis in Any kDTM Mini-PROTEAN TGX precast gel (Bio-Rad) and transferred to a PVDF membrane (Bio-Rad) ...
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bioRxiv - Systems Biology 2020Quote: ... and used 500 ng - 1 ug RNA to make cDNA using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA). 0.1 of cDNA (i.e ...
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bioRxiv - Plant Biology 2021Quote: ... ∼1 ug of RNA from resulting extraction was used as template in standard cDNA synthesis reaction (BioRad iScript cDNA Synthesis Kit). 200 ng of resulting cDNA was used in qRT-PCR reactions to quantify POL expression levels (PowerUp SYBR Green 5x Master Mix-Thermo) ...
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bioRxiv - Molecular Biology 2020Quote: ... The quality and concentration of the RNA was assessed and first strand cDNA synthesised from 1 ug using the iScript Select cDNA Synthesis Kit (Bio-Rad) and a 1:1 mixture of Oligo (dT ...
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bioRxiv - Cancer Biology 2023Quote: ... 10-15 ug of protein lysates were run on 4– 20% Criterion™ TGX™ Precast Midi Protein Gel (Bio-Rad) and then transferred to polyvinylidene difluoride membranes ...
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bioRxiv - Neuroscience 2023Quote: ... Protein (10 ug) from each sample was treated with ß-mercaptoethanol and subsequently electrophoresed on precast 4%–10% gradient gels (Biorad), as previously described (Alcantara et al. ...
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bioRxiv - Neuroscience 2023Quote: ... 10 ug of protein from cortical samples and 45ug of muscle samples were diluted in 4x Laemmli sample buffer (Bio-Rad). P3 brain samples were already directly resuspended in 4x Laemmli sample buffer ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were sonicated and heated at 95°C for 5 minutes before their separation on a 4-15% gradient SDS PAGE gel (Biorad, 4-15%). Proteins were transferred from gel to nitrocellulose membrane using semi-dry method ...
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bioRxiv - Molecular Biology 2021Quote: 5 μg of cheRNA or total RNA for each condition were denatured at 95°C for 5 min and transferred to a Hybond-XL membrane (Amersham) in a Bio-Dot® Microfiltration Apparatus (Bio-Rad) following manufacturer’s intructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... and MgCl2 [12.5 mM]) and annealed by cooling the solution from 95 to 12 °C over 30 min in a thermal cycler (Bio-Rad, T100). Detailed descriptions for the assembly of individual gate complexes are described in the supplementary information ...