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Citations for Bio-Rad :
351 - 400 of 1595 citations for Erythromycin 90 95% Erythromycin A N N Dimethyl 13C2 ~90% 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Samples were boiled at 95 °C for 5 minutes and separated on precast 4-20% Criterion TGX gels (Bio-Rad). The competition of IA-rhodamine was analyzed by in-gel fluorescent signal using a ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Bioengineering 2023Quote: ... and the supernatant was incubated at 95°C for 10 minutes in the presence of Laemmli sample buffer (Bio-Rad). Proteins (20 μg per sample ...
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bioRxiv - Cell Biology 2023Quote: ... and boiled for 5 min at 95 C and loaded onto an or 4-20% TGX stain-free polyacrylamide gels (Biorad), followed by standard western blotting procedures ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were boiled at 95 °C for 5 min before being loaded onto a polyacrylamide gel (Cat# 4561094, Bio-Rad). Proteins were blotted onto a PVDF membrane (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... All samples collected were heated to 95°C for 5 minutes before resolving on a 12% mini-PROTEAN precast gel (BioRad). A Western blot using polyclonal EssR antiserum was conducted following the protocol outlined in the Western blot method section below ...
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bioRxiv - Cell Biology 2023Quote: ... and boiled for 5 min at 95 C and loaded onto an or 4-20% TGX stain-free polyacrylamide gels (Biorad), followed by standard western blotting procedures ...
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bioRxiv - Molecular Biology 2024Quote: ... 95 °C) and 1.25 µg sample was resolved on a 4-12% acrylamide Criterion XT Bis-Tris gel (Bio-Rad) in MOPS buffer at 100 V for 20 min and subsequently at 150V for 190 min on ice along with PageRuler™ Plus Prestained Protein Ladder (Thermo Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... using 45 cycles of 15 sec at 95°C and 30 sec at 60°C in a CFX96 cycler (Biorad). Data were normalized to the transcript encoding PP2A subunit A3 (At1g13320 ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.01% bromophenol blue) and denaturated 5 min at 95°C before protein separation by SDS–PAGE on 4-15% precast gels (Biorad).
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bioRxiv - Microbiology 2024Quote: ... the samples were boiled at 95°C for 10 minutes and resolved on a TGX stain-free gel (Bio-Rad) for SDS-PAGE analysis ...
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bioRxiv - Plant Biology 2024Quote: ... using 45 cycles of 15 s at 95°C and 30 s at 60°C in a CFX96 cycler (Biorad). Primers are listed in Additional file 1.
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bioRxiv - Molecular Biology 2024Quote: ... and heat denatured at 95°C for 10 min before loading onto a 4-15% Tris-glycine gel (Bio-Rad) and electrophoresed at 125 V ...
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bioRxiv - Physiology 2023Quote: ... Protein extracts were denatured at 95 °C for 10 min and separated on 10% SDS-PAGE gels (1610156, Bio-Rad) at 80 V for ∼40 min followed by 110 V for ∼70 min ...
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bioRxiv - Microbiology 2024Quote: Protein samples were incubated at 95°C for 10 minutes before being resolved on TGX Stain-free gels (Bio-Rad). The proteins were transferred onto 0.2 μm nitrocellulose membranes using Trans-Blot Turbo Transfer (Bio-Rad) ...
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bioRxiv - Plant Biology 2020Quote: ... containing 2 ml of anion exchange resin AGMP-1M (100-200 mesh, chloride form) (BIO-RAD, 1411841). The resin was first cleansed three times with 10 ml 18.2Ω water followed by 7 ml 0.5N HNO3 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... genomic DNA was extracted using 250 mL of 5% Chelex 100 resin (Bio-Rad Laboratories, Hercules, CA) and 3 mL of proteinase K (20 mg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant from the cell lysate was collected by centrifuging the cell lysate at 10,000 g for 10 min at 4°C and further diluted in lysis buffer to 8.0 ug/ul using a modified Bradford protein quantification assay (Bio-Rad), flash-frozen in liquid N2 ...
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bioRxiv - Physiology 2020Quote: ... equal amounts of protein (20 ug) were loaded in 4-12% - Criterion XT Bis-Tris protein gels (Bio-Rad, CA) and transferred to nitrocellulose membranes ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were boiled with SDS-PAGE sample loading buffer (6X SDS) and 30 ug of protein was loaded to each well of a 10% Tris-glycine SDS-PAGE gel (BioRad). Proteins were transferred to a PVDF membrane (Millipore) ...
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bioRxiv - Physiology 2023Quote: ... and left ventricle of wild-type C57 Bl6 mice was incubated with PC2 antibody (10 ug, Alomone laboratories) overnight complexed to magnetic protein G beads (Biorad). Following 3 rounds of washing ...
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bioRxiv - Cancer Biology 2023Quote: ... Western blots were loaded at 2.5 to 50 ug total protein per lane in 4-20% gradient pre-cast polyacrylamide gels (#3450033, Bio-Rad). Gels were also loaded with the Dual pre-stained protein standards (#1610374 ...
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bioRxiv - Microbiology 2020Quote: ... One or two micrograms of the concentrated viruses were heated at 95 °C for 5 min before being resolved on 4-20% SDS-PAGE (Bio-Rad) using the Novex™ Sharp Pre-stained Protein Standard (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants were mixed with SDS sample buffer containing DTT and incubated for 5 min at 95°C prior to fractionation by SDS-PAGE analysis on 4%–15% Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). Transfer to nitrocellulose membranes and probing for Western blot analysis was as described previously ...
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bioRxiv - Evolutionary Biology 2020Quote: Samples were heated at 95°C for 10 minutes before loading into 7.5% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) and running at 150V for approximately 90 minutes at 4°C in 1X tris-gylcine running buffer ...
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bioRxiv - Cell Biology 2020Quote: ... to pH 8.88 first for 5 min at 95 °C followed by 60 °C for 3 h on a T100 Thermal Cycler (Bio-Rad) with the heated lid set to 105 °C ...
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bioRxiv - Microbiology 2019Quote: ... Samples were boiled for 5 minutes at 95°C before separation on a gradient 4-20% SDS-PAGE gel (Bio-Rad). Samples were then transferred to nitrocellulose membrane using standard methods for semi-dry transfer (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μg of lysate was heat denatured for 3 minutes at 95°C for 3 minutes and resolved on a pre-cast 4-15 % SDS-PAGE gels (Bio-Rad) at 200V for 45 minutes in 1X running buffer (25mM Tris pH 8.3 ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... Protein was eluted from beads by heating for 5 min at 95 °C in 1X Laemmli Buffer (Bio-Rad, Hercules, CA). Retrieved protein was detected by standard immunoblotting techniques using the following antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were heated at a rate of 0.5 °C per minute from 25 °C to 95 °C and fluorescence signals were recorded with the CFX Connect Real-Time PCR Detection System (Bio-Rad). The melting temperatures were analyzed using the derivative plots of the melting curve.
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bioRxiv - Bioengineering 2021Quote: ... 15 µg of the extracted protein samples were boiled for 5 minutes at 95 °C in 4X laemlli buffer (BioRad; 1610747), run on mini-PROTEAN TGX precast gels (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... was added to each well and samples were heated from 25 to 95 °C in 1 °C steps of 1 min each in a CFX96 Touch™ Real-Time PCR Detection System (BioRad). Excitation/emission filters of 492 and 516 nm were used to monitor the fluorescence increase resulting from binding of the Sypro Orange to exposed hydrophobic regions of the unfolding MsSepFcore ...
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bioRxiv - Microbiology 2022Quote: ... SYPRO orange fluorescence data in relative fluorescence unit (RFU) was collected from 10 °C to 95 °C using CFX Connect Real-Time PCR Detection System (Bio-Rad). The temperature corresponding to the lowest point of the first derivative −d(RFU)/dT was determined to be the Tm.
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bioRxiv - Cancer Biology 2022Quote: ... then 40 cycles of 15 seconds at 95°C and 60 seconds at 60°C using CFX96 Touch Real-Time PCR Detection System (Bio-rad). A standard curve was derived from the serial dilutions of DNA template following concentrations ...
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bioRxiv - Biochemistry 2022Quote: ... SYPRO orange fluorescence data in relative fluorescence unit (RFU) was collected from 10 °C to 95 °C using CFX Connect Real-Time PCR Detection System (Bio-Rad). The temperature corresponding to the lowest point of the first derivative ...
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bioRxiv - Cell Biology 2022Quote: ... denatured in 2x Laemmli buffer (5 min at +95°C) and 20 µg were loaded per well of Mini-PROTEAN Precast Gel (Bio-Rad). The protein transfer was done with the Trans-Blot Turbo system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were incubated at 95 °C for 5 minutes prior to being resolved on a 4 – 15% Mini-PROTEAN TGX Precast Protein Gels (Bio-rad) and transferred onto a methanol activated Immobilon-P PVDF membrane (Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with beta-mercaptoethanol and denaturated at 95 °C for 5 min before loading on a 12 % PAA precast gel (#4561044, Bio-Rad). The gel was run at 70 V for 15 min and then 100 V for 1 h ...
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bioRxiv - Immunology 2020Quote: ... Samples were heated at 95°C for 3 minutes and run on a 8 – 16% or 4 – 20% TGX-Criterion gel (Bio-Rad). The separated proteins were transferred to PVDF membranes and blocked in PBS containing 0.2% fish skin gelatin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... Cell viability was assessed by Trypan blue staining (typically 85– 95% viable) and counted using an automatic cell counter (Bio-Rad). Cells are platedon 100 mg/mL poly-L-ornithine and 10 mg/ mL laminin coated plates at 50,000 cells/cm2 and incubated overnight ...
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bioRxiv - Immunology 2020Quote: ... Protein for immunoblotting was eluted from 25% of beads by heating at 95°C for 5 minutes in 2X Laemmli sample buffer (Bio-Rad). RNA was extracted from the remaining 75% of beads using TRIzol (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... The lysates were boiled for 5 min at 95°C in 1x XT sample buffer containing 1x XT reducing agent (both from Bio-Rad) and resolved on 4-12% Bis-Tris XT precast gels (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: Protein samples were denatured for 5 min at 95 °C and run on Mini or Midi TGX-Stain-free precast gels (Bio-rad) at 110V for 60 min (mini gels ...
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bioRxiv - Neuroscience 2019Quote: ... and then eluted by boiling in SDS lysis buffer for 5 minutes at 95°C and resolved by SDS-PAGE (Bio-rad) gel ...
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Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeabilitybioRxiv - Molecular Biology 2020Quote: ... The incubation was terminated by boiling the samples at 95°C for 5 minutes in 1X Laemmli sample buffer (Bio-Rad) supplemented with 10% β-mercaptoethanol (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... proteins were denaturized in SDS-PAGE protein loading buffer for 5 minutes at 95°C and resolved by SDS-PAGE on 4-12% bis-tris Criterion XT Precast gels (Bio-rad), transferred to a PVDF membrane and blocked for at least 1h in 1% Casein blocking buffer (Sigma) ...
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bioRxiv - Neuroscience 2019Quote: ... 30 μg of each protein sample was denatured and reduced by boiling at 95°C for 5 min in 10% (v/v) 2-mercaptoethanol-containing Laemmli sample buffer (Bio-Rad). Samples were resolved by SDS-PAGE with a precast Tris-glycine gel (Bio-Rad ...
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bioRxiv - Biochemistry 2019Quote: ... was transferred to a new tube with 50 μL 2 M HCl and boiled for 2 hours at 95°C in a C1000 thermocycler (Bio-Rad) to hydrolyze all glucans to glucose ...
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bioRxiv - Bioengineering 2020Quote: ... followed by 40 cycles of 95°C for 5 s and 60°C for 30 s (Bio-Rad CFX manager 3.1). Quantification of mRNA levels of different genes was performed using the 2−ΔΔct method ...
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bioRxiv - Microbiology 2021Quote: ... Expression and secretion samples were incubated at 95 °C for 10 minutes and then resolved on TGX Stain-free gel (Bio-Rad). Next ...
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bioRxiv - Genomics 2020Quote: ... heated at 95°C for 10 min and run on a Mini-protean TGX 4%-20% polyacrylamide gel (Biorad, 456-1095). The proteins were transferred onto PVDF membranes using the Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad ...